CRISPR Impact on Plant Breeding

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1 Agriculture Division of DowDuPont CRISPR Impact on Plant Breeding Urban Ag Academy Dr. Kevin Diehl Global Seed Regulatory Platform Director Corteva Agriscience, Agriculture Division of DowDuPont July 20, 2018

2 Our Purpose To enrich the lives of those who produce and those who consume, ensuring progress for generations to come Agriculture Division of DowDuPont 2

3 Agriculture Division s Key Technology Platforms Agriculture Division s Key Technology Platforms Key Technology Platforms forms BREEDING CRISPR BIOTECH CROP PROTECTION SEED APPLIED TECHNOLOGY DIGITAL SOLUTIONS AGRONOMIC SOLUTIONS Agriculture Division of DowDuPont

4 CRISPR-Cas are custom-designed molecular scissors to introduce a break at the target DNA sequence Cas9 nuclease (DNA cutting enzyme) Guide RNA is designed to bring Cas9 enzyme to the targeted DNA sequence Cas9 enzyme binds to the targeted DNA sequence and makes the DNA double strand break Guide RNA homologous to the target DNA sequence DNA break is repaired through endogenous cellular mechanism CRISPR-Cas applications Agriculture Division of DowDuPont 4

5 How Can We Use CRISPR-Cas? DELETE The plant does not have desired output. The plant does have desired output. EDIT The plant has tolerance to drought. The plant has high tolerance to drought. SEARCH/REPLACE The plant is susceptible to disease. The plant is resistant to disease. Agriculture Division of DowDuPont 5

6 Plant Breeding Develops Product Diversity Agriculture Division of DowDuPont 6

7 Deploying CRISPR-Cas Advanced Plant Breeding Broadly Deploying CRISPR-Cas Advanced Plant Breeding Broadly DISEASE RESISTANCE YIELD & YIELD STABILITY DROUGHT TOLERANCE OUTPUT TRAITS MATURITY CORN SOY CANOLA RICE WHEAT SUNFLOWER Products, benefits and concepts described herein will not be offered for sale or distribution until completion of field testing and applicable regulatory reviews. Agriculture Division of DowDuPont

8 CRISPR-Cas Waxy Corn Starch High amylopectin starch corn Phenotype due to mutation in Wx1 gene: starch synthase catalyzing amylose biosynthesis In commercial cultivation since mid-1940 s Variety of uses in food and paper-making industries Agriculture Division of DowDuPont 8

9 Examples of Traits Developed by CRISPR-Cas Gene Editing RICE WHEAT Corn: Reduced phytate Waxy starch Disease resistance Yield components improvement Fragrant High amylose CAMELINA Powdery mildew resistance Low gluten Virus resistance Canker resistance Soybean: Improved oil quality Delayed flowering Improved oil quality Powdery mildew resistant Partenocarpic (seedless) High GABA Longer shelf life Agriculture Division of DowDuPont 9

10 Solutions Around the Corner? Cacao Innovative Genomics Institute The fungus will always be there. But if the plants can [become] resistant, we don t have to spray so much. Rong Di, a plant pathologist and molecular biologist, Rutgers University, USA Agriculture Division of DowDuPont 10

11 Solutions Around the Corner? geneticliteracyproject.org Citrus greening has slashed US orange production in half over the past decade, and threatens to destroy the US$3.3-billion industry entirely. Source: Agriculture Division of DowDuPont 11

12 Corteva TM Agriscience Collaborations through Open Innovation CIMMYT Targeting Maize Lethal Necrosis Disease Danforth Center Targeting Cassava Improvement ICRISAT Targeting food security crops such as sorghum and millet Agriculture Division of DowDuPont 12

13 What Enables Innovation? Science Technology Technical capabilities Intellectual property Collaborations Policy Regulatory frameworks Social license Societal value Trust Who benefits? Transparency Agriculture Division of DowDuPont 13