Food Safety in Japan

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1 Food Safety in Japan Tadahiro Nagata Incorporated Administrative Agency National Food Research Institute Tsukuba, Ibaraki, Japan

2 Food Safety in Japan Current situation in Japan National Food Research Institute Outline Microbial Sensor Development Acrylamide Analysis Rice Breed Identification MAFF project for Food Safety

3 Consumer Concerns Safe? Milk products cause food poisoning affecting 14,780 people BSE 70% drop in demand for beef. The minister of agriculture admitted to grossly misleading reports about the handling of BSE infected cattle.

4 A Drastic Change for Securing Food Safety and Consumer Confidence Food Safety Basic Law Enactment on 23 May, 2003 Basic principle Protection of people s life and health Securing of safety in the food supply chain Appropriate response to scientific knowledge and international development

5 Functional separation of risk assessment and risk management Food Safety Commission Establishment on 1 July, 2003 Risk Assessment Body Subsidiary body of the Cabinet Office

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7 Introduction of NFRI

8 History of NFRI 1934 Established as Rice Utilization Laboratory in Ministry of Agriculture and Forestry 1947 Renamed as Foods Research Institute 1972 Renamed as National Food Research Institute 1979 Moved from Tokyo to Tsukuba 2001 NFRI became an incorporated administrative agency (IAA) IAA under the supervision of MAFF

9 Organization in Research Field Food Function Division (5 labs) Food Safety and Quality Division (5 labs +2 team) Food Material Division (5 labs +1 team) Food Engineering Division (5 labs +1 team) Applied Microbiology Division (4 labs) Biological Function Division (4 labs ) Analytical Science Division (4 labs+1 team)

10 Food Safety & Credibility Research Ensuring Food Safety Microbial Sensor Development Timely Analytical Data Acrylamide Analysis Ensuring the Labeling System Rice Breed Identification

11 Food Poisoning by Microbes in Japan Total outbreaks by microbes Salmonella spp. Staphylococcus aureus Clostridium botulinum Vibrio Pathogenic E. coli E.coli O157:H7 Other pathogenic E. coli Clostridium perfringens Bacillus cereus Yersinia enterocolitica Campylobacter jejuni/coli Nagvibrio Cholera SRSV(Norwalk-like virus) , , , ,

12 Microbial Food Safety from Farm to Fork Production GAP Food Processing GMP SSOP HACCP High risk point! Store GHP Inappropriate temp. control! Distribution Low temperature control refrigeration freezing Minimally processed and Ready-to-eat foods Home GHP

13 Strategy for microbial sensor development Reqirements: Safety, Simplicity, Low cost, Easy detection Temperature (Analyte) Temp. Input Principles: Fermentation activity Glucose EtOH + CO2 Output Commercial Baker s yeast (High freeze tolerance) Sensor (a multilayer laminate film bag) Components (Safe: Food additives) Yeast cells, Glucose, Glycerol, Yeast extract Gas Production Visual detection

14 The time history exposed to the inappropriate temperature can be deduced by the amount of gas produced in the microbial sensor. 500 Gas generation (CO2) 12 Gas prodution (μl) Temperature ( ) Time (hr) 2

15 This is not food. Sensor making machine Please refrain from eating and drinking when the amount of bubble gets over the blue line. Sensor (working model)

16 Sensor cutting and attaching machine Sensor (Control) Gas production

17 Food Safety in Japan Current situation in Japan National Food Research Institute Outline Microbial Sensor Development Acrylamide Analysis Rice Breed Identification MAFF project for Food Safety

18 Simple History of Acrylamide problems Apr. 24, 2002 Publication of the Swedish study May 17 Publication of the British study Jun Joint FAO/WHO consultation, Geneva Aug. 14 Recommendation of Action Value; 1ppm Sep. 20 FDA Draft Action Plan for Acrylamide in Food Oct. 31 Publication of the Data in Japan by NFRI and NIHS (Natl Inst. Health Science)

19 Preparation procedure for LC-MS/MS and GC-MS

20 Comparison of Values by LC-MS/MS and GC-MS

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22 Food Safety in Japan Current situation in Japan National Food Research Institute Outline Microbial Sensor Development Acrylamide Analysis Rice Breed Identification MAFF project for Food Safety

23 Consumer Concerns Safe? Milk products cause food poisoning affecting 14,780 people BSE 70% drop in demand for beef. The minister of agriculture admitted to grossly misleading reports about the handling of BSE infected cattle. False labeling; breed, place-of-origin,,, Mislabeled; GMO food products GMO-containing foods as non-gmo foods Reliable?

24 食品表示に関する消費者の意識調査 結果報告 速報版 Recent Consumer Attitudes Regarding 速報版 の公表について Food Labelling 平成 14 年 June 6 月 日内閣府 Recent Change Confidence in labelling GMO or Non-GMO Place-of-origin, organic? newly concerned previously concerned unconcerned

25 Rice Breed Identification Sample DNA Extraction PCR Gel Electrophoresis

26 Koshi-hikari Posi-Kit & Nega-Kit ポジキット ネガキットによる混米の検出例 A M M Posi-Kit B M M Nega-Kit 1 4 C M M Nega-Kit 2 5 Koshi-hikari A : ポジキット 100 B : ネガキット (1) 50 C : ネガキット 25 (2) 0 % Mutsu-homare 1: むつほまれ混米 % 2:10% :25% :50% % 5:75% 6:100%

27 For Credibility MAFF project for Food Safety 3.1 million Euros (2005) Traceability, Verification of labeling Ubiquitous technique, DNA analysis (Breed?), Organic? Cultivation? Previously frozen? For Safety Risk analysis Microbe, Mycotoxin, Acrylamide, Heavy metal,,, Rick communication, Laboratory quality assurance Method validation, Proficiency testing,, Integrated Research Program for Functionality and Safety of Food Toward an Establishment of Healthy Diet

28 Thank you Acknowledgements Dr. Shinichi Kawamoto, Dr.Ken ichi Ohtsubo