Using Resynchronization Programs

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1 Using Resynchronization Programs WITH ESTRUS DETECTION & ECONOMIC EVALUATION Todd Bilby, Ph.D. Associate Director, Ruminant Technical Services Merck Animal Health The Fatty Acid Forum sponsored by

2 Achieving High Fertility Through Multiple Programs Reproduction programs of award winners at Dairy Cattle Reproduction Council Award Level 100% HD HD/ 100% Platinum ( ) Gold Silver Bronze Total Platinum award winners from ranged in pregnancy rate 29-40%!

3 Presynchronization Protocols Presynch/Ovsynch Ovsynch PGF PGF GnRH PGF GnRH

4 Presynchronization Protocols Presynch/Ovsynch Ovsynch PGF PGF GnRH PGF GnRH 14d 51 11d 72 73

5 Presynchronization Protocols Presynch/Ovsynch Ovsynch PGF PGF GnRH PGF GnRH 14d 51 11d 72 73

6 Presynchronization Protocols Presynch/Ovsynch Ovsynch PGF PGF GnRH PGF GnRH 14d 51 83% Heats: 2 6 d after PGF

7 Presynchronization Protocols Presynch/Ovsynch Ovsynch PGF PGF GnRH PGF GnRH 14d EC Day

8 Presynchronization Protocols Double Ovsynch GnRH Ovsynch PGF GnRH GnRH Ovsynch PGF GnRH

9 Presynchronization Protocols Double Ovsynch GnRH Ovsynch PGF GnRH GnRH Ovsynch PGF GnRH Sun Sun Tue Tue Tue Thu Fri

10 Presynchronization Protocols Double Ovsynch GnRH Ovsynch PGF GnRH GnRH Ovsynch PGF GnRH EC Day 7

11 Presynchronization Protocols Double Ovsynch G6G PGF GnRH GnRH Ovsynch PGF GnRH Sun Tue Mon Mon Wed Thu EC Day 6

12 Presynchronization Protocols Double Ovsynch G6G PGF GnRH GnRH Ovsynch PGF GnRH Sun Tue Mon Mon Wed Thu Presynch increases fertility 5 7% units EC Day 6

13 Resynchronization What role does estrus detection play in resynchronization of dairy cows?

14 Timing of Initiation of Resynch Timing of initiation of resynch according to the phase of the estrus cycle. Day of Estrus Day GnRH Day 29 Day 5 Day 6 Day 7 Day 8 Day 9

15 Pattern of Return to Estrus of Lactating Dairy Cows Cows Days After AI

16 Pattern of Return to Estrus of Lactating Dairy Cows 1400 Cows Proportion re-inseminated 20 24d after AI (DC305 data) 537 lactating cows (80 lb/d) = 58% 1,016 lactating cows (90 lb/d) = 53% 3,379 lactating cows (82 lb/d) = 40% 4,971 lactating cows (84 lb/d) = 55% 5,778 lactating cows (70 lb/d) = 52% 8,856 lactating cows (81 lb/d) = 55% 52% Days After AI

17 Different Presynch Protocols Before Resynch Ovsynch GnRH 7 d Ovsynch Ovsynch 7 d Ovsynch PGF 12 d Ovsynch Presynch increased fertility 5 7 % units but some programs increase the inter AI breeding interval 7 13 d Preg Check Dewey et al., 2010; Silva et. al., 2007; Giordano et al., 2012

18 Fertility with GnRH and PGF No difference on fertility whether GnRH or PGF was used as Presynch Overall P/AI, 66 d PGF 11 d Ovsynch 30% PGF 7 d Ovsynch 27% GnRH 7 d Ovsynch 26% NPD d 32 Bruno et al., 2014

19 GnRH Reduced Number of Cows Detected in Estrus % Estrus Detected/AI Timed AI GGPG P7GPG P11GPG Bruno et al. (2014)

20 GnRH Reduced Number of Cows Detected in Estrus % 71% Estrus Detected/AI Timed AI GGPG P7GPG P11GPG Bruno et al. (2014)

21 Intervals Between AI With PGF Non-inseminated (%) Interval Between AI PG 7 d = 36 d PG 11 d = 39 d GnRH = 42 d Interval between study enrollment and AI (days) PG 11 d GnRH PG 7 d

22 Pattern of Return to Estrus of Lactating Dairy Cows 1400 Cows Proportion re-inseminated 20 24d after AI (DC305 data) 537 lactating cows (80 lb/d) = 58% 1,016 lactating cows (90 lb/d) = 53% 3,379 lactating cows (82 lb/d) = 40% 4,971 lactating cows (84 lb/d) = 55% 5,778 lactating cows (70 lb/d) = 52% 8,856 lactating cows (81 lb/d) = 55% 52% Days After AI

23 Pattern of Return to Estrus of Lactating Dairy Cows Cows Days After AI

24 Pattern of Return to Estrus of Lactating Dairy Cows Cows GNRH Days After AI

25 Pattern of Return to Estrus of Lactating Dairy Cows Cows GNRH Days After AI

26 Simulation of 10 Reproductive Programs Estimate the impact of reproductive programs on the net return per cow per year ($/cow/year). Two milk prices were evaluated: RP RP Compliance to ED ED Accuracy 1 ED ED ED ED /ED /ED /ED /ED 95 60

27 Impact of Reproductive Program on Pregnancy Rate and Return per Cow per Year 400 Milk: $15/cwt 30 $/cow/year d cycle pregnancy rate, % Characteristics of the Reproductive Programs Compliance ED ED accuracy Pregnancy rate, %

28 Reproductive Programs Compared A. 100% Presynch Ovsynch Ovsynch VWP B. Combined = Estrus Detection + ED + AI ED + AI Presynch Ovsynch Ovsynch VWP Giordano et al. (2012)

29 100% vs. Varying Levels of Estrus Detection/ 35 25% CR 30% CR 35% CR 25 Net Value ($/cow/y) % 40% 50% 60% 70% 80% Reproductive Program

30 100% vs. Varying Levels of Estrus Detection/ 35 25% CR 30% CR 35% CR 25 Net Value ($/cow/y) to -17 $/cow/yr % 40% 50% 60% 70% 80% Reproductive Program

31 100% vs. Varying Levels of Estrus Detection/ 35 25% CR 30% CR 35% CR 25 Net Value ($/cow/y) to +10 $/cow/yr % 40% 50% 60% 70% 80% Reproductive Program

32 100% vs. Varying Levels of Estrus Detection/ 35 25% CR 30% CR 35% CR 25 Net Value ($/cow/y) to +32 $/cow/yr % 40% 50% 60% 70% 80% Reproductive Program

33 Conclusions 1. Presynchronization before Ovsynch improves fertility Whether its first AI or Resynch! 2. GnRH/hCG will reduce estrus and PGF will promote. Don t use protocols that will reduce estrus if your trying to detect estrus! Estrus Fertility is normally 5 10% units better than 3. Initiating Synch with a GnRH/hCG before d 25 will greatly reduce estrus and not improve fertility 4.Shortening your Resynch interval is good but not at the expense of reducing fertility 5. Estrus detection is important but must have accurate detection for good conception rates 6. protocols are an insurance tool

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