Growth regulation in winter cereals using Medax Top

Transcription

1 Faculty of Agricultural Sciences, Department of Integrated Pest Management Growth regulation in winter cereals using Medax Top - Sprayed when the crop was at growth stage BBCH Crop: winter cereals Trial no.: 25-1, 55-1, -1, (efficacy) Carried out for: BASF A/S Ref. Jørgen Larsen c/o BASF Services Europe GmbH Entity Berlin Germany Created by: Herbicid Trial group, Research Centre Flakkebjerg University of Aarhus Faculty of Agricultural Sciences Department of Integrated Pest Management Research Centre Flakkebjerg DK-42 Slagelse Date: 11 December 2 Flakkebjerg Research Centre Flakkebjerg Flakkebjerg 1 Tel: DK-42 Slagelse Fax:

2 Faculty of Agricultural Sciences, Department of Integrated Pest Management Title: Trial number: Growth regulation in winter cereals using Medax Top. Sprayed when the crop was at growth stage BBCH. 25-1, 55-1, -1, (efficacy) Number of pages: 81 Trial leader: Research assistant: Karen Eberhardt Henriksen Anders Almskou-Dahlgaard, Steen Sørensen, Verner Lindberg, Poul Hansen, Henrik Jespersen and Karen Jensen. Trial period: September 28 - September 2. Performance criterion: Publication: Preformed according to GEP (Good Efficacy Practice) (Appendix 4: GEP certificate). The use of the results can only take place after the General Provisions for commissioned assignments. September 1, 1997 (Appendix 3). Trial leader I, the undersigned hereby declare that this work was performed under my directions and in accordance with The Principles of Good Experimental Practice. The study was conducted according to the procedures herein described and this report represents a true and accurate record of the results obtained. Date: Karen Eberhardt Henriksen Research Centre Flakkebjerg Flakkebjerg 2 Tel: DK-42 Slagelse Fax:

3 Faculty of Agricultural Sciences, Department of Integrated Pest Management Contents: SUMMARY:...5 GENERAL EVALUATION OF MEDAX TOP CROP BBCH...5 HIGHT REDUCTION IN WINTER WHEAT...9 HIGHT REDUCTION IN TRITICALE...1 HIGHT REDUCTION IN WINTER BARLEY...11 TRIAL LOCATION OF TRIAL:...12 PLAN WITH ACTIVE INGREDIENT GENERAL EVALUATION:...14 DESCRIPTION OF THE TRIAL...15 VISUAL ASSESSMENTS...17 RAW DATA...2 TRIAL LOCATION OF TRIAL:...25 PLAN WITH ACTIVE INGREDIENT GENERAL EVALUATION:...27 DESCRIPTION OF THE TRIAL...28 VISUAL ASSESSMENTS...3 RAW DATA...34 TRIAL LOCATION OF TRIAL:...39 PLAN WITH ACTIVE INGREDIENT....4 GENERAL EVALUATION:...41 DESCRIPTION OF THE TRIAL...42 VISUAL ASSESSMENTS...44 RAW DATA...47 TRIAL LOCATION OF TRIAL:...5 PLAN WITH ACTIVE INGREDIENT GENERAL EVALUATION:...52 DESCRIPTION OF THE TRIAL...53 VISUAL ASSESSMENTS...55 RAW DATA...59 Research Centre Flakkebjerg Flakkebjerg 3 Tel: DK-42 Slagelse Fax:

4 Faculty of Agricultural Sciences, Department of Integrated Pest Management APPENDIX A...64 VISUAL ASSESSMENTS PHYTOTOXICITY OF MEDAX TOP USED IN WINTER CEREALS CROP BBCH...65 INCREASED YIELD IN WINTER CEREALS USING MEDAX TOP...71 APPENDIX B READING GUIDE THE CLIMATE IN FLAKKEBJERG EXTRACT FROM»GENERAL PROVISIONS FOR COMMISSIONED ASSIGNMENTS«SEPTEMBER GEP CERTIFICATE Research Centre Flakkebjerg Flakkebjerg 4 Tel: DK-42 Slagelse Fax:

5 Faculty of Agricultural Sciences, Department of Integrated Pest Management Summary: General evaluation of Medax Top crop BBCH Trials, comparing Medax Top (5 g/l prohexadione + 3 g/l mepiquat) with Moddus M (25 g/l trinexapac ethyl) and with Terpal (155 g/l ethephon + 35 g/l mepiquat-chlorid), were carried out in Denmark in 2. 1 efficacy trial in winter barley (25) 1 efficacy trial in winter rye (55) 1 efficacy trial in winter triticale () 1 efficacy trial in winter wheat (245) Winter barley The efficacy trial (25) was sprayed 1 May 2 at crop growth stage Two dosages (½ N, 1/1 N) of the products were tested and compared. Results winter barley: Height reduction - trial Rreduction % ½ N 1/1 N Medax Top Moddus M Terpal Dose There was no significant difference between the reduction of height neither related to products nor to doses. Research Centre Flakkebjerg Flakkebjerg 5 Tel: DK-42 Slagelse Fax:

6 Faculty of Agricultural Sciences, Department of Integrated Pest Management Winter rye The efficacy trial (55) was sprayed 27 April 2 at crop growth stage 38. Two dosages (½ N, 1/1 N) of the products were tested and compared. Results winter rye: Height reduction - trial Reduction % Medax Top Moddus M Terpal ½ N Dose 1/1 N The treatment with 1.5 l/ha Medax Top (1/1 N) resulted in significant crop height reduction compared to any of the other treatments. The treatment with.75 l/ha Medax Top (½ N) resulted in significant crop height reduction compared to the treatments with (½ N) Moddus M and (½ N) Terpal. Research Centre Flakkebjerg Flakkebjerg 6 Tel: DK-42 Slagelse Fax:

7 Faculty of Agricultural Sciences, Department of Integrated Pest Management Winter tricicale The efficacy trial () was sprayed 11 May 2 at crop growth stage Two dosages (½ N, 1/1 N) of the products were tested and compared. Results winter triticale: Height reduction - trial Reduction % ½ N Dose 1/1 N Medax Top Moddus M Terpal The treatment with.75 l/ha Medax Top (½ N) resulted in significant crop height reduction compared to the treatments with (½ N) Moddus M and (½ N) Terpal. The treatment with 1.5 l/ha Medax Top (1/1 N) resulted in significant crop height reduction compared to the treatments with (1/1 N) Moddus M and (1/1 N) Terpal. Research Centre Flakkebjerg Flakkebjerg 7 Tel: DK-42 Slagelse Fax:

8 Faculty of Agricultural Sciences, Department of Integrated Pest Management Winter wheat The efficacy trial (245) was sprayed 13 May 2 at crop growth stage Two dosages (½ N, 1/1 N) of the products were tested and compared. Results winter wheat: Height reduction - trial Reduction % 8 6 Medax Top Moddus M Terpal 4 2 ½ N Dose 1/1 N The treatment with.75 l/ha Medax Top (½ N) resulted in significant crop height reduction compared to the treatments with (½ N) Moddus M and (½ N) Terpal. The treatment with 1.5 l/ha Medax Top (1/1 N) resulted in significant crop height reduction compared to the treatments with (1/1 N) Moddus M and (1/1 N) Terpal. Research Centre Flakkebjerg Flakkebjerg 8 Tel: DK-42 Slagelse Fax:

9 Faculty of Agricultural Sciences, Department of Integrated Pest Management Hight reduction in winter wheat Medax Top compared to Moddus M and Terpal in Winter wheat. Hight reduction BBCH 59 BBCH 65 BBCH 75 Terpal 1,5 l/ha Terpal,75 l/ha Moddus,5 l/ha Treatments Moddus,25 l/ha Medax top 1,5 l/ha Medax top,75 lha Karakter for high reduction in % Research Centre Flakkebjerg Flakkebjerg 9 Tel: DK-42 Slagelse Fax:

10 Faculty of Agricultural Sciences, Department of Integrated Pest Management Hight reduction in Triticale Medax Top compared to Moddus M and Terpal in Triticale. Hight reduction BBCH 65 BBCH 75 Terpal 1,5 l/ha Terpal,75 l/ha Moddus M,5 l/ha Treatments Moddus M,25 l/ha Medax top 1,5 l/ha Medax top,75 l/ha Hight reduction in % Research Centre Flakkebjerg Flakkebjerg 1 Tel: DK-42 Slagelse Fax:

11 Faculty of Agricultural Sciences, Department of Integrated Pest Management Hight reduction in winter barley Medax Top compared to Moddus M and Terpal in Winter Rye High reduction BBCH 59 BBCH 65 Terpal 1,5 l/ha Terpal,75 l/ha Moddus M,5 l/ha Treatment Moddus M,25 l/ha Medax Top 1,5 l/ha Medax Top,75 l/ha High reduction in % Research Centre Flakkebjerg Flakkebjerg 11 Tel: DK-42 Slagelse Fax:

12 Faculty of Agricultural Sciences, Department of Integrated Pest Management Trial 25-1 Location of Trial: 25-1 Fuglebjerg Research Centre Flakkebjerg Flakkebjerg 12 Tel: DK-42 Slagelse Fax:

13 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Trt Treatment Form Form Rate Other Other Growth Appl Comment No. Type Name Conc Type Rate Unit Rate Rate Unit Stage Code 1 1 HERB Medax Top 35 SC,75 L/ha 263 G A/HA Crop A -mepiquat 3,64 -prohexadion 5,17 2 HERB Medax Top 35 SC 1,5 L/ha 525 G A/HA Crop A -mepiquat 3 1,29 -prohexadion 5,214 3 HERB Moddus M 25 EC,25 L/ha 62,5 G A/HA Crop A -trinexapac-ethyl 25,25 4 HERB Moddus M 25 EC,5 L/ha 125 G A/HA Crop A -trinexapac-ethyl 25,5 5 HERB Terpal 46 FL,75 L/ha 345 G A/HA Crop A -ethephon 155,253 -mepiquat-chlorid 35,497 6 HERB Terpal 46 FL 1,5 L/ha 69 G A/HA Crop A -ethephon 155,55 -mepiquat-chlorid 35,99 7 CHK Untreated Check 1/2 N 1/1 N 1/2 N 1/1 N 1/2 N 1/1 N Replications: 4, Untreated treatments: 7, Conduct under GLP/GEP: Yes (GEP with audit trail), Design: Factorial, Factors: 2, (Herbicide, Rate of Treatment), Treatment units: Treated 'Plot' size, Dry Form. Unit: %, Treated 'Plot' size Width: 2,5 meters, Treated 'Plot' size Length: 5 meters, Application volume: 15 L/ha, Mix size: 2 litres, Format definitions: G-All7.def, G-All7.frm 13

14 Faculty of Agricultural Sciences, Department of Integrated Pest Management General Evaluation: The trial was established as an efficacy trial (growth regulation) in winter barley with the objective of testing Medax Top at different doses. The treatment with Medax Top was carried out when the winter barley was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 5% at a half dose and by 7% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. At the time of full earing a delay in growth was recorded at half and quarter doses compared with untreated. On 9 July it was recorded that the treatment had resulted in a reduction in the breaking of the ears compared with untreated. The treatment with Moddus M was carried out when the winter barley was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment there was no reduction in the height of the straw at a half dose compared with untreated. At a normal dose the straw height was increased by 5% compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. No delay in growth was recorded at the time of full earing at half and quarter doses compared with untreated. The treatment with Terpal was carried out when the winter barley was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment there was no reduction in the height of the straw at a half dose compared with untreated. At a normal dose the straw height had been reduced by 2% compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. No delay in growth was recorded at the time of full earing at half and quarter doses compared with untreated. There was a significant difference in the breaking of the ears at one of the treatments. At the measuring of the straw height 3 and 6 weeks after treatment there was a significant difference between 2 treatments but none compared with untreated. Research Centre Flakkebjerg Flakkebjerg 14 Tel: DK-42 Slagelse Fax:

15 (25-1) Site Description Page 1 of 2 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF General Trial Information Study Director: Karen Eberhardt Henriksen Title: Research leader Investigator: Karen Eberhardt Henriksen Title: Research leader Discipline: P growth regulator (plant) Trial Status: F one-year/final Initiation Date: City: Slagelse State/Prov.: Sjælland Postal Code: 42 Country: DEN Denmark Trial Location Conducted Under GEP: X Guideline Description 1. EPPO Growth Regulation in Cerials Personnel Study Director: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Investigator: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Cooperator: Hans Nicolaisen Address 1: Storgårdsvej 5 Phone No.: City: Fuglebjerg State/Prov: Sjælland Mobile No.: Postal Code: 425 Country: DEN Denmark Cooperator/Landowner Crop Description Crop 1: HORVW Hordeum vulgare (winter) Barley Winter Variety: Pelican Description: CONVENTIONAL BBCH Scale: BCER Planting Date: Planting Method: DRILLE drilled Rate, Unit: 167 KG/HA Depth, Unit: 5 cm Perennial Age, Unit: 1 YEAR Row Spacing, Unit: 12,5 cm Seed Bed: MEDIUM medium Site and Design Plot Width, Unit: 2,5 m Site Type: FIELD field Plot Length, Unit: 5 m Experimental Unit: 28 PLOT plot Plot Area, Unit: 12,5 m2 Tillage Type: CONTIL conventional-till Replications: 4 Study Design: FACTOR Factorial % Slope:, Untreated Arrangement: INCLUDED single control randomized in each block Previous Crops Year 1. Barley Spring 28 Soil Description Description Name: Textureanalyze % Sand: 77,8 % OM: 2,2 Texture: GRSL gravelly sandy 15 loam

16 (25-1) Site Description Page 2 of 2 % Silt: 8,8 ph: 6,8 Soil Name: 5 % Clay: 11,3 CEC: 7,3 Fert. Level: G good Soil Drainage: G Analyzed By: AGROLAB GmbH Application Description Application Date: Time of Day: 1-11 Application Method: A SPRAY Application Timing: Application Placement: BROFOL Applied By: Air Temperature, Unit: SCS % Relative Humidity: 5 14 C Wind Velocity, Unit: 2 MS Wind Direction: Dew Presence (Y/N): Soil Moisture: % Cloud Cover: SE N no VD/VD/M Crop Stage At Each Application Crop 1 Code, BBCH Scale: Stage Scale Used: A HORVW BCER BBCH Stage Majority, Percent: 37 5 Stage Maximum, Percent: 38 5 Application Equipment Appl. Equipment: Equipment Type: Operating Pressure, Unit: 2.6 Nozzle Type: Nozzle Size: Nozzle Spacing, Unit: Nozzles/Row: 5 A Sprayer A SPLOVO Hardi Boom ID: V/1 Boom Length, Unit: Boom Height, Unit: Ground Speed, Unit: Carrier: ATM LD CM 2,5 M 5 CM 4,5 KPH Selfprop. Spray Volume, Unit: 15 L/HA Mix Size, Unit: 2 Liters Tank Mix (Y/N): N no 16

17 (25-1) AOV Means Table Page 1 of 3 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Pest Code Crop Code HORVW HORVW HORVW HORVW HORVW Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type PHYGEN EARING LODING HEIGHT PHYGEN Rating Unit PERCENT BBCH PERCENT CM PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH Trt-Eval Interval 24 DA-A 24 DA-A 24 DA-A 24 DA-A 45 DA-A Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop a 58 a a 74 ab a 2 Medax Top 1,5 L/ha Crop a 57 b a 7 b a 3 Moddus M,25 L/ha Crop a 59 a a 8 ab a 4 Moddus M,5 L/ha Crop a 59 a a 84 a a 5 Terpal,75 L/ha Crop a 59 a a 78 ab a 6 Terpal 1,5 L/ha Crop a 59 a a 78 ab a 7 Untreated Check a 59 a a 79 ab a LSD (P=.5),,5, 7,5, CV,,59, 6,5, Replicate F,,3, 1,734, Replicate Prob(F) 1,,825 1,,1959 1, Treatment F, 14,6, 3,369, Treatment Prob(F) 1,,1 1,,21 1, Means followed by same letter do not significantly differ (P=.5, Student-Newman-Keuls) 17

18 (25-1) AOV Means Table Page 2 of 3 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) HORVW HORVW HORVW HORVW HORVW PLANT C PLANT C PLANT C EAR C STRAW C LODING HEIGHT LODING WILTIN WILTIN PERCENT CM PERCENT PERCENT PERCENT BBCH BBCH BBCH BBCH BBCH 45 DA-A 45 DA-A 69 DA-A 69 DA-A 69 DA-A a 81 a a 2 a a a 79 a a 1 b a a 85 a a 2 a a a 89 a a 2 a a a 85 a a 2 a a a 83 a a 2 a a a 85 a a 2 a a, 6,6,,7,, 5,34, 32,7,,,924, 7,125, 1,,4494 1,,24 1,, 2,254, 4,5, 1,,849 1,,59 1, 18

19 (25-1) AOV Means Table Page 3 of 3 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code HORVW, BCER, Hordeum vulgare (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury EARING = Earing/Skridning HEIGHT = height WILTIN = wilting Rating Unit PERCENT = percent BBCH = -99 BBCH scale CM = centimeter Crop Stage Majority 61 = Beginning of flowering: first anthers visible 83 = Early dough Crop Stage Scale BBCH = BBCH uniform plant stages 19

20 (25-1) Assessment Data Summary Page 1 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code HORVW HORVW HORVW HORVW HORVW HORVW HORVW HORVW HORVW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Crop Name Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C EAR C Rating Date Rating Type PHYGEN EARING LODING HEIGHT PHYGEN LODING HEIGHT LODING WILTIN Rating Unit PERCENT BBCH PERCENT CM PERCENT PERCENT CM PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI VLI VLI VLI SCS SCS SCS VLI VLI Days After First/Last Applic Trt-Eval Interval 24 DA-A 24 DA-A 24 DA-A 24 DA-A 45 DA-A 45 DA-A 45 DA-A 69 DA-A 69 DA-A Plant-Eval Interval 246 DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = Medax Top 1,5 L/ha Crop Mean = Moddus M,25 L/ha Crop Mean = Moddus M,5 L/ha Crop Mean = Terpal,75 L/ha Crop Mean = Terpal 1,5 L/ha Crop Mean =

21 (25-1) Assessment Data Summary Page 2 of 5 Crop Code HORVW HORVW HORVW HORVW HORVW HORVW HORVW HORVW HORVW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Hordeum vulgare (winter) Crop Name Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Winter barley Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C EAR C Rating Date Rating Type PHYGEN EARING LODING HEIGHT PHYGEN LODING HEIGHT LODING WILTIN Rating Unit PERCENT BBCH PERCENT CM PERCENT PERCENT CM PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI VLI VLI VLI SCS SCS SCS VLI VLI Days After First/Last Applic Trt-Eval Interval 24 DA-A 24 DA-A 24 DA-A 24 DA-A 45 DA-A 45 DA-A 45 DA-A 69 DA-A 69 DA-A Plant-Eval Interval 246 DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 7 Untreated Check Mean =

22 (25-1) Assessment Data Summary Page 3 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth HORVW BCER Hordeum vulgare (winter) Winter barley STRAW C -7-2 WILTIN PERCENT BBCH 3 VLI DA-A 291 DP-1 No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = 2 Medax Top 1,5 L/ha Crop Mean = 3 Moddus M,25 L/ha Crop Mean = 4 Moddus M,5 L/ha Crop Mean = 5 Terpal,75 L/ha Crop Mean = 6 Terpal 1,5 L/ha Crop Mean = 22

23 (25-1) Assessment Data Summary Page 4 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth HORVW BCER Hordeum vulgare (winter) Winter barley STRAW C -7-2 WILTIN PERCENT BBCH 3 VLI DA-A 291 DP-1 No. Name Rate Unit Stage Plot 7 Untreated Check Mean = 23

24 (25-1) Assessment Data Summary Page 5 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: 25-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code HORVW, BCER, Hordeum vulgare (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury EARING = Earing/Skridning HEIGHT = height WILTIN = wilting Rating Unit PERCENT = percent BBCH = -99 BBCH scale CM = centimeter Crop Stage Majority 61 = Beginning of flowering: first anthers visible 83 = Early dough Crop Stage Scale BBCH = BBCH uniform plant stages Assessed By VLI = Verner Lindberg Plant-Eval Interval 246 DP-1 = DP-1 = DP-1 = Footnote 1: Den 25. maj 2: Ved bedømmelse af gennemskridning er vækststadie bedømt udfra akses plasering i forhold til fanebladet. Ikke byggens faktiske udviklingsstadie. Erik og Verner Footnote 2: ; Steen Footnote 3: Den 9. juli 2: Verner 24

25 Faculty of Agricultural Sciences, Department of Integrated Pest Management Trial 55-1 Location of Trial: 55-1 Slagelse Research Centre Flakkebjerg Flakkebjerg 25 Tel: DK-42 Slagelse Fax:

26 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Trt Treatment Form Form Rate Other Other Growth Appl Comment No. Type Name Conc Type Rate Unit Rate Rate Unit Stage Code 1 1 HERB Medax Top 35 SC,75 L/ha 263 G A/HA Crop A -mepiquat 3,64 -prohexadion 5,17 2 HERB Medax Top 35 SC 1,5 L/ha 525 G A/HA Crop A -mepiquat 3 1,29 -prohexadion 5,214 3 HERB Moddus M 25 EC,25 L/ha 62,5 G A/HA Crop A -trinexapac-ethyl 25,25 4 HERB Moddus M 25 EC,5 L/ha 125 G A/HA Crop A -trinexapac-ethyl 25,5 5 HERB Terpal 46 FL,75 L/ha 345 G A/HA Crop A -ethephon 155,253 -mepiquat-chlorid 35,497 6 HERB Terpal 46 FL 1,5 L/ha 69 G A/HA Crop A -ethephon 155,55 -mepiquat-chlorid 35,99 7 CHK Untreated Check 1/2 N 1/1 N 1/2 N 1/1 N 1/2 N 1/1 N Replications: 4, Untreated treatments: 7, Conduct under GLP/GEP: Yes (GEP with audit trail), Design: Factorial, Factors: 2, (Herbicide, Rate of Treatment), Treatment units: Treated 'Plot' size, Dry Form. Unit: %, Treated 'Plot' size Width: 2,5 meters, Treated 'Plot' size Length: 5 meters, Application volume: 15 L/ha, Mix size: 2 litres, Format definitions: G-All7.def, G-All7.frm 26

27 Faculty of Agricultural Sciences, Department of Integrated Pest Management General Evaluation: The trial was established as an efficacy trial (growth regulation) in winter rye with the objective of testing Medax Top at different doses. The treatment with Medax Top was carried out when the winter rye was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 12% at a half dose and by 21% at a normal dose compared with untreated. Lodging was assessed several times. At harvest, the score in untreated was 13, which is equivalent of approx. 5% of the straws leaning. At a half dose the score was 8, which is equivalent of approx. 3% of the straws leaning. At a normal dose the score was 4, which is equivalent of approx. 15% of the straws leaning. At the time of full earing in untreated, growth stage 59, a delay in growth was recorded at half and normal doses. At a half dose the winter rye was at growth stage 57 and at a normal dose the winter rye was at growth stage 55. At harvest, a reduction in straw breaking was recorded at half and normal doses compared with untreated. The treatment with Moddus M was carried out when the winter rye was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 3% at a half dose and by 12% at a normal dose compared with untreated. Lodging was assessed several times. At harvest, the score in untreated was 13, which is equivalent of approx. 5% of the straws leaning. At a half dose the score was 1, which is equivalent of approx. 4% of the straws leaning. At a normal dose the score was 8, which is equivalent of approx. 3% of the straws leaning. At the time of full earing in untreated, growth stage 59, a delay in growth was recorded at half and normal doses. At a half dose the winter rye was at growth stage 58 and at a normal dose the winter rye was at growth stage 57. At harvest, a reduction in straw breaking was recorded at a normal dose compared with untreated. The treatment with Terpal was carried out when the winter rye was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 3% at half and normal doses compared with untreated. Lodging was assessed several times. At harvest, the score in untreated was 13, which is equivalent of approx. 5% of the straws leaning. At a half dose the score was 11, which is equivalent of approx. 45% of the straws leaning. At a normal dose the score was 1, which is equivalent of approx. 4% of the straws leaning. At the time of full earing in untreated, growth stage 59, a delay in growth (growth stage 58) was recorded at a normal dose. At harvest, a reduction in straw breaking was recorded at a normal dose compared with untreated. At the measuring of the straw height 3 and 6 weeks after treatment there was a significant difference between several treatments and compared with untreated. At the assessment of lodging 14 days before harvest there was a significant difference between several treatments and compared with untreated, but there was no difference at harvest. There was no significant difference in breaking between treatments and compared with untreated. Research Centre Flakkebjerg Flakkebjerg 27 Tel: DK-42 Slagelse Fax:

28 (55-1) Site Description Page 1 of 2 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF General Trial Information Study Director: Karen Eberhardt Henriksen Title: Research leader Investigator: Karen Eberhardt Henriksen Title: Research leader Discipline: P growth regulator (plant) Trial Status: F one-year/final Initiation Date: City: Slagelse State/Prov.: Sjælland Postal Code: 42 Country: DEN Denmark Trial Location Conducted Under GEP: X Guideline Description 1. EPPO Growth Regulation in cerials Personnel Study Director: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Investigator: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Cooperator: Steen Jørgensen Johannesdal Address 1: Johannesdalvej 15 Phone No.: City: Slagelse State/Prov: Sjælland Mobile No.: Postal Code: 42 Country: DEN Denmark Cooperator/Landowner Crop Description Crop 1: SECCW Secale cereale (winter) Rye Winter Variety: Rasant + Carotop Description: CONVENTIONAL BBCH Scale: BCER Planting Date: Planting Method: DRILLE drilled Rate, Unit: 95 KG/HA Depth, Unit: 5 cm Perennial Age, Unit: 1 YEAR Row Spacing, Unit: 12 cm Seed Bed: FINE fine Site and Design Plot Width, Unit: 2,5 m Site Type: FIELD field Plot Length, Unit: 5 m Experimental Unit: 28 PLOT plot Plot Area, Unit: 12,5 m2 Tillage Type: CONTIL conventional-till Replications: 4 Study Design: FACTOR Factorial % Slope:, Untreated Arrangement: INCLUDED single control randomized in each block Previous Crops Year 1. Barley Spring 28 Soil Description Description Name: Textureanalyze % Sand: 8,2 % OM: 1,9 Texture: COSL coarse sandy loam % Silt: 8,1 ph: 6,3 Soil Name: 3 28

29 (55-1) Site Description Page 2 of 2 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF % Clay: 9,8 CEC: 5,8 Fert. Level: G good Soil Drainage: G Analyzed By: AGROLAB GmbH Application Description A Application Date: Time of Day: - 1 Application Method: SPRAY Application Timing: 38 Application Placement: BROFOL Applied By: SCS/VLI Air Temperature, Unit: 15 C % Relative Humidity: 67 Wind Velocity, Unit: 2 MS Wind Direction: SE Dew Presence (Y/N): N no Soil Moisture: VD/VD/D % Cloud Cover: 5 Crop Stage At Each Application A Crop 1 Code, BBCH Scale: SECCW BCER Stage Scale Used: BBCH Stage Majority, Percent: 38 1 Application Equipment A Appl. Equipment: Sprayer A Equipment Type: SPLOVO Operating Pressure, Unit: 2.6 ATM Nozzle Type: Hardi Nozzle Size: LD15-11 Nozzle Spacing, Unit: 5 CM Nozzles/Row: 5 Boom ID: V/1 Boom Length, Unit: 2,5 M Boom Height, Unit: 5 CM Ground Speed, Unit: 4,5 KPH Carrier: Selfprop. Spray Volume, Unit: 15 L/HA Mix Size, Unit: 2 Liters Tank Mix (Y/N): N no 29

30 (55-1) AOV Means Table Page 1 of 4 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Pest Code Crop Code SECCW SECCW SECCW SECCW SECCW Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type EARING LODING HEIGHT HEIGHT PHYGEN Rating Unit BBCH PERCENT CM CM PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH Trt-Eval Interval 18 DA-A 18 DA-A 18 DA-A 37 DA-A 37 DA-A Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop c a 93 b 131 b a 2 Medax Top 1,5 L/ha Crop d a 8 c 117 c a 3 Moddus M,25 L/ha Crop b a 14 a 144 a a 4 Moddus M,5 L/ha Crop c a 96 b 13 b a 5 Terpal,75 L/ha Crop ab a 1 a 144 a a 6 Terpal 1,5 L/ha Crop b a 16 a 143 a a 7 Untreated Check 59 a a 1 a 148 a a LSD (P=.5),6, 4,6 5,6, CV,69, 3,1 2,74, Replicate F,825, 5,671 1,682, Replicate Prob(F),4971 1,,65,3 1, Treatment F 48,, 46,655 35,2, Treatment Prob(F),1 1,,,1 1, Means followed by same letter do not significantly differ (P=.5, Student-Newman-Keuls) 3

31 (55-1) AOV Means Table Page 2 of 4 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) SECCW SECCW SECCW SECCW SECCW PLANT C PLANT C PLANT C PLANT STRAW LODING LODING LODING PHYGEN WILTIN PERCENT PERCENT PERCENT PERCENT PERCENT BBCH BBCH BBCH BBCH BBCH 37 DA-A 58 DA-A 8 DA-A 94 DA-A 94 DA-A a a 6 cd a 2 a a a 1 e a 2 a a a 11 ab a 3 a a a 4 de a 2 a a a 9 bc a 4 a a a 9 bc a 2 a a a 14 a a 3 a,, 3,5, 1,4,, 3,7, 38,87,, 4,875, 6,427 1, 1,,118 1,,38,, 13,286, 3,165 1, 1,,1 1,,267 31

32 (55-1) AOV Means Table Page 3 of 4 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) SECCW SECCW EAR PLANT WILTIN LOGDING PERCENT PERCENT BBCH BBCH 94 DA-A 94 DA-A a 8 ab a 4 b a 1 ab a 8 ab a 11 ab a 1 ab a 13 a, 5,6, 41,89, 3,83 1,,278, 2,447 1,,659 32

33 (55-1) AOV Means Table Page 4 of 4 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code SECCW, BCER, Secale cereale (winter), = IE Part Rated PLANT = plant STRAW = straw EAR = ear C = Crop is Part Rated Rating Type EARING = Earing/Skridning HEIGHT = height PHYGEN = phytotoxicity - general / injury WILTIN = wilting Rating Unit BBCH = BBCH plant stages PERCENT = percent CM = centimeter Crop Stage Majority 59 = End of heading: inflorescence fully emerged 77 = Late milk 83 = Early dough 89 = Fully ripe: grain hard, difficult to divide with thumbnail Crop Stage Scale BBCH = BBCH uniform plant stages 33

34 (55-1) Assessment Data Summary Page 1 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Crop Name Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT STRAW Rating Date Rating Type PHYGEN LODING HEIGHT HEIGHT PHYGEN LODING LODING LODING PHYGEN WILTIN Rating Unit PERCENT PERCENT Cm CM PERCENT PERCENT PERCENT PERCENT PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By SCS SCS SCS VLI/HEJE VLI/HEJE VLI/HEJE SCS VLI HEJE / SCS HEJE / SCS Days After First/Last Applic Trt-Eval Interval 18 DA-A 18 DA-A 18 DA-A 37 DA-A 37 DA-A 37 DA-A 58 DA-A 8 DA-A 94 DA-A 94 DA-A Plant-Eval Interval 237 DP DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = Medax Top 1,5 L/ha Crop Mean = Moddus M,25 L/ha Crop Mean = Moddus M,5 L/ha Crop Mean = Terpal,75 L/ha Crop Mean = Terpal 1,5 L/ha Crop Mean =

35 (55-1) Assessment Data Summary Page 2 of 5 Crop Code SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW SECCW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Secale cereale (winter) Crop Name Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Winter rye Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT STRAW Rating Date Rating Type PHYGEN LODING HEIGHT HEIGHT PHYGEN LODING LODING LODING PHYGEN WILTIN Rating Unit PERCENT PERCENT Cm CM PERCENT PERCENT PERCENT PERCENT PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By SCS SCS SCS VLI/HEJE VLI/HEJE VLI/HEJE SCS VLI HEJE / SCS HEJE / SCS Days After First/Last Applic Trt-Eval Interval 18 DA-A 18 DA-A 18 DA-A 37 DA-A 37 DA-A 37 DA-A 58 DA-A 8 DA-A 94 DA-A 94 DA-A Plant-Eval Interval 237 DP DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 7 Untreated Check Mean =

36 (55-1) Assessment Data Summary Page 3 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth SECCW SECCW BCER BCER Secale cereale (winter) Secale cereale (winter) Winter rye Winter rye EAR PLANT WILTIN LOGDING PERCENT PERCENT BBCH BBCH 5 5 HEJE / SCS HEJE / SCS DA-A 94 DA-A 313 DP DP-1 No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = 2 Medax Top 1,5 L/ha Crop Mean = 3 Moddus M,25 L/ha Crop Mean = 4 Moddus M,5 L/ha Crop Mean = 5 Terpal,75 L/ha Crop Mean = 6 Terpal 1,5 L/ha Crop Mean = 1 36

37 (55-1) Assessment Data Summary Page 4 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth SECCW SECCW BCER BCER Secale cereale (winter) Secale cereale (winter) Winter rye Winter rye EAR PLANT WILTIN LOGDING PERCENT PERCENT BBCH BBCH 5 5 HEJE / SCS HEJE / SCS DA-A 94 DA-A 313 DP DP-1 No. Name Rate Unit Stage Plot 7 Untreated Check Mean = 13 37

38 (55-1) Assessment Data Summary Page 5 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: 55-1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code SECCW, BCER, Secale cereale (winter), = IE Part Rated PLANT = plant STRAW = straw EAR = ear C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury HEIGHT = height WILTIN = wilting Rating Unit PERCENT = percent Cm = centimeter Crop Stage Majority 59 = End of heading: inflorescence fully emerged 77 = Late milk 83 = Early dough 89 = Fully ripe: grain hard, difficult to divide with thumbnail Crop Stage Scale BBCH = BBCH uniform plant stages Assessed By VLI/HEJE = Verner LIndberg/ Henrik Jespersen VLI = Verner Lindberg HEJE / SCS = Henrik Jespersen / Steen C. Sørensen Plant-Eval Interval 237 DP-1 = DP-1 = DP-1 = DP-1 = DP-1 = Footnote 1: ; Erik, Steen Footnote 2: 1/6 Verner og Henrik Footnote 3: ; Verner, Steen. Footnote 4: Den 16. juli 2: Verner Footnote 5: 3/7-2 Steen, Henrik 38

39 Faculty of Agricultural Sciences, Department of Integrated Pest Management Trial -1 Location of Trial: -1 Korsør Research Centre Flakkebjerg Flakkebjerg 39 Tel: DK-42 Slagelse Fax:

40 Growth regulation in winter cereals - Efficcacy Trial ID: -1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Trt Treatment Form Form Rate Other Other Growth Appl Comment No. Type Name Conc Type Rate Unit Rate Rate Unit Stage Code 1 1 HERB Medax Top 35 SC,75 L/ha 263 G A/HA Crop A -mepiquat 3,64 -prohexadion 5,17 2 HERB Medax Top 35 SC 1,5 L/ha 525 G A/HA Crop A -mepiquat 3 1,29 -prohexadion 5,214 3 HERB Moddus M 25 EC,25 L/ha 62,5 G A/HA Crop A -trinexapac-ethyl 25,25 4 HERB Moddus M 25 EC,5 L/ha 125 G A/HA Crop A -trinexapac-ethyl 25,5 5 HERB Terpal 46 FL,75 L/ha 345 G A/HA Crop A -ethephon 155,253 -mepiquat-chlorid 35,497 6 HERB Terpal 46 FL 1,5 L/ha 69 G A/HA Crop A -ethephon 155,55 -mepiquat-chlorid 35,99 7 CHK Untreated Check 1/2 N 1/1 N 1/2 N 1/1 N 1/2 N 1/1 N Replications: 4, Untreated treatments: 7, Conduct under GLP/GEP: Yes (GEP with audit trail), Design: Factorial, Factors: 2, (Herbicide, Rate of Treatment), Treatment units: Treated 'Plot' size, Dry Form. Unit: %, Treated 'Plot' size Width: 2,5 meters, Treated 'Plot' size Length: 5 meters, Application volume: 15 L/ha, Mix size: 2 litres, Format definitions: G-All7.def, G-All7.frm 4

41 Faculty of Agricultural Sciences, Department of Integrated Pest Management General Evaluation: The trial was established as an efficacy trial (growth regulation) in triticale with the objective of testing Medax Top at different doses. The treatment with Medax Top was carried out when the triticale was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 22% at a half dose and by 32% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. Neither was any breaking of straws or ears recorded at half and normal doses. The treatment with Moddus M was carried out when the triticale was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 8% at a half and normal doses. Lodging was assessed several times, but no lodging was recorded at normal and half doses. Neither was any breaking of straws or ears recorded at half and normal doses. The treatment with Terpal was carried out when the triticale was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 11% at a half dose and by 18% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. Neither was any breaking of straws or ears recorded at half and normal doses. At the measuring of the straw height 3 and 6 weeks after treatment there was a significant difference between several treatments and compared with untreated. Research Centre Flakkebjerg Flakkebjerg 41 Tel: DK-42 Slagelse Fax:

42 (-1) Site Description Page 1 of 2 Growth regulation in winter cereals - Efficcacy Trial ID: -1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF General Trial Information Study Director: Karen Eberhardt Henriksen Title: Research leader Investigator: Karen Eberhardt Henriksen Title: Research leader Discipline: P growth regulator (plant) Trial Status: F one-year/final Initiation Date: City: Slagelse State/Prov.: Sjælland Postal Code: 42 Country: DEN Denmark Trial Location Conducted Under GEP: X Guideline Description 1. EPPO Growth Regulation in cerials Personnel Study Director: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Investigator: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Cooperator: Michael Lundgård Address 1: Agervej 73 Tjæreby Phone No.: City: Korsør State/Prov: Sjælland Mobile No.: Postal Code: 422 Country: DEN Denmark Cooperator/Landowner Crop Description Crop 1: TTLWI Triticale (winter) Triticale Winter Variety: Algato Description: CONVENTIONAL BBCH Scale: BCER Planting Date: Planting Method: DRILLE drilled Rate, Unit: 13 KG/HA Depth, Unit: 5 cm Perennial Age, Unit: 1 YEAR Row Spacing, Unit: 12,5 cm Seed Bed: FINE fine Site and Design Plot Width, Unit: 2,5 m Site Type: FIELD field Plot Length, Unit: 5 m Experimental Unit: 28 PLOT plot Plot Area, Unit: 12,5 m2 Tillage Type: CONTIL conventional-till Replications: 4 Study Design: FACTOR Factorial % Slope:, Untreated Arrangement: INCLUDED single control randomized in each block Previous Crops Year 1. Barley Spring 28 Soil Description Description Name: Textureanalyze % Sand: 8,8 % OM: 3, Texture: COSL coarse sandy 42 loam

43 (-1) Site Description Page 2 of 2 % Silt: 7,5 ph: 5,2 Soil Name: 3 % Clay: 8,7 CEC: 4,7 Fert. Level: G good Soil Drainage: G Analyzed By: AGROLAB GmbH Application Description Application Date: Time of Day: Application Method: A SPRAY Application Timing: Application Placement: BROFOL Applied By: Air Temperature, Unit: SCS % Relative Humidity: C Wind Velocity, Unit: 5 MS Wind Direction: Dew Presence (Y/N): Soil Moisture: % Cloud Cover: NW N no VD/D/M Crop Stage At Each Application Crop 1 Code, BBCH Scale: Stage Scale Used: A TTLWI BCER BBCH Stage Majority, Percent: 38 6 Stage Maximum, Percent: 43 4 Application Equipment Appl. Equipment: Equipment Type: Operating Pressure, Unit: 2.6 Nozzle Type: Nozzle Size: Nozzle Spacing, Unit: Nozzles/Row: 5 A Sprayer A SPLOVO Hardi Boom ID: V/1 Boom Length, Unit: Boom Height, Unit: Ground Speed, Unit: Carrier: ATM LD CM 2,5 M 5 CM 4,5 KPH Selfprop. Spray Volume, Unit: 15 L/HA Mix Size, Unit: 2 Liters Tank Mix (Y/N): N no 43

44 (-1) AOV Means Table Page 1 of 3 Growth regulation in winter cereals - Efficcacy Trial ID: -1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Pest Code Crop Code TTLWI TTLWI TTLWI TTLWI TTLWI Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type PHYGEN LODING HEIGHT PHYGEN LODING Rating Unit PERCENT PERCENT CM PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH Trt-Eval Interval 23 DA-A 23 DA-A 23 DA-A 44 DA-A 44 DA-A Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop a a 75 c a a 2 Medax Top 1,5 L/ha Crop a a 65 d a a 3 Moddus M,25 L/ha Crop a a 93 ab a a 4 Moddus M,5 L/ha Crop a a 9 ab a a 5 Terpal,75 L/ha Crop a a 87 b a a 6 Terpal 1,5 L/ha Crop a a 79 c a a 7 Untreated Check a a 95 a a a LSD (P=.5),, 5,4,, CV,, 4,36,, Replicate F,, 1,885,, Replicate Prob(F) 1, 1,,1682 1, 1, Treatment F,, 36,556,, Treatment Prob(F) 1, 1,,1 1, 1, Means followed by same letter do not significantly differ (P=.5, Student-Newman-Keuls) 44

45 (-1) AOV Means Table Page 2 of 3 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) TTLWI TTLWI TTLWI TTLWI PLANT C PLANT C EAR C STRAW C HEIGHT LODING WILTIN WILTIN CM PERCENT PERCENT PERCENT BBCH BBCH BBCH BBCH 44 DA-A 66 DA-A 74 DA-A 74 DA-A 8 d a a a 7 e a a a 95 ab a a a 95 ab a a a 92 bc a a a 85 cd a a a 13 a a a a 7,5,,, 5,74,,,,756,,,,5332 1, 1, 1, 18,759,,,,1 1, 1, 1, 45

46 (-1) AOV Means Table Page 3 of 3 Growth regulation in winter cereals - Efficcacy Trial ID: -1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code TTLWI, BCER, Triticale (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury HEIGHT = height WILTIN = wilting Rating Unit PERCENT = percent CM = centimeter Crop Stage Majority 65 = Full flowering: 5% of anthers mature 83 = Early dough 87 = Hard dough: grain content solid. Fingernail impression held Crop Stage Scale BBCH = BBCH uniform plant stages 46

47 (-1) Assessment Data Summary Page 1 of 3 Growth regulation in winter cereals - Efficcacy Trial ID: -1 Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Crop Name Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C EAR C STRAW C Rating Date Rating Type PHYGEN LODING HEIGHT PHYGEN LODING HEIGHT LODING WILTIN WILTIN Rating Unit PERCENT PERCENT CM PERCENT PERCENT CM PERCENT PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI/HEJE VLI/HEJE VLI/HEJE SCS SCS SCS VLI HEJE / SCS HEJE / SCS Days After First/Last Applic Trt-Eval Interval 23 DA-A 23 DA-A 23 DA-A 44 DA-A 44 DA-A 44 DA-A 66 DA-A 74 DA-A 74 DA-A Plant-Eval Interval 245 DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = Medax Top 1,5 L/ha Crop Mean = Moddus M,25 L/ha Crop Mean = Moddus M,5 L/ha Crop Mean = Terpal,75 L/ha Crop Mean = Terpal 1,5 L/ha Crop Mean =

48 (-1) Assessment Data Summary Page 2 of 3 Crop Code TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI TTLWI BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Triticale (winter) Crop Name Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Winter triticale Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C EAR C STRAW C Rating Date Rating Type PHYGEN LODING HEIGHT PHYGEN LODING HEIGHT LODING WILTIN WILTIN Rating Unit PERCENT PERCENT CM PERCENT PERCENT CM PERCENT PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI/HEJE VLI/HEJE VLI/HEJE SCS SCS SCS VLI HEJE / SCS HEJE / SCS Days After First/Last Applic Trt-Eval Interval 23 DA-A 23 DA-A 23 DA-A 44 DA-A 44 DA-A 44 DA-A 66 DA-A 74 DA-A 74 DA-A Plant-Eval Interval 245 DP DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 7 Untreated Check Mean = Crop Code TTLWI, BCER, Triticale (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury HEIGHT = height WILTIN = wilting Rating Unit PERCENT = percent CM = centimeter Crop Stage Majority 65 = Full flowering: 5% of anthers mature 83 = Early dough 87 = Hard dough: grain content solid. Fingernail impression held Crop Stage Scale BBCH = BBCH uniform plant stages Assessed By VLI/HEJE = Verner LIndberg/ Henrik Jespersen VLI = Verner Lindberg HEJE / SCS = Henrik Jespersen / Steen C. Sørensen Plant-Eval Interval 245 DP-1 = DP-1 = DP-1 =

49 (-1) Assessment Data Summary Page 3 of DP-1 = Footnote 1: 1/6- Verner og Henrik Footnote 2: ; Verner, Steen Footnote 3: Den 16. juli 2: Verner Footnote 4: 24/7- Steen og Henrik 49

50 Faculty of Agricultural Sciences, Department of Integrated Pest Management Trial Location of Trial: Sandved Research Centre Flakkebjerg Flakkebjerg 5 Tel: DK-42 Slagelse Fax:

51 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Trt Treatment Form Form Rate Other Other Growth Appl Comment No. Type Name Conc Type Rate Unit Rate Rate Unit Stage Code 1 1 HERB Medax Top 35 SC,75 L/ha 263 G A/HA Crop A -mepiquat 3,64 -prohexadion 5,17 2 HERB Medax Top 35 SC 1,5 L/ha 525 G A/HA Crop A -mepiquat 3 1,29 -prohexadion 5,214 3 HERB Moddus M 25 EC,25 L/ha 62,5 G A/HA Crop A -trinexapac-ethyl 25,25 4 HERB Moddus M 25 EC,5 L/ha 125 G A/HA Crop A -trinexapac-ethyl 25,5 5 HERB Terpal 46 FL,75 L/ha 345 G A/HA Crop A -ethephon 155,253 -mepiquat-chlorid 35,497 6 HERB Terpal 46 FL 1,5 L/ha 69 G A/HA Crop A -ethephon 155,55 -mepiquat-chlorid 35,99 7 CHK Untreated Check 1/2 N 1/1 N 1/2 N 1/1 N 1/2 N 1/1 N Replications: 4, Untreated treatments: 7, Conduct under GLP/GEP: Yes (GEP with audit trail), Design: Factorial, Factors: 2, (Herbicide, Rate of Treatment), Treatment units: Treated 'Plot' size, Dry Form. Unit: %, Treated 'Plot' size Width: 2,5 meters, Treated 'Plot' size Length: 5 meters, Application volume: 15 L/ha, Mix size: 2 litres, Format definitions: G-All7.def, G-All7.frm 51

52 Faculty of Agricultural Sciences, Department of Integrated Pest Management General Evaluation: The trial was established as an efficacy trial (growth regulation) in winter wheat with the objective of testing Medax Top at different doses. The treatment with Medax Top was carried out when the winter wheat was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 7% at a half dose and by 13% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. At earing in untreated, growth stage 55, a delay in growth (growth stage 53) was recorded at a normal dose. No breaking of straws or ears was recorded at half and normal doses. The treatment with Moddus M was carried out when the winter wheat was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 1% at a half dose and 2% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. At earing in untreated, growth stage 55, no delay in growth was recorded at half and normal doses. Nor was any breaking of straws or ears recorded at half or normal doses. The treatment with Terpal was carried out when the winter wheat was at growth stage Measuring of the straw height was carried out 3 and 6 weeks after treatment. 6 weeks after treatment the height of the straw had been reduced by 3% at a half dose and 6% at a normal dose compared with untreated. Lodging was assessed several times, but no lodging was recorded at normal and half doses. At earing in untreated, growth stage 55, a delay in growth was recorded at a normal dose at which time the winter wheat was at growth stage 54 in normal dose treatments. No breaking of straws or ears was recorded at half and normal doses compared with untreated. At the measuring of the straw height 3 and 6 weeks after treatment there was a significant difference between several treatments and compared with untreated. Research Centre Flakkebjerg Flakkebjerg 52 Tel: DK-42 Slagelse Fax:

53 (245-1) Site Description Page 1 of 2 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF General Trial Information Study Director: Karen Eberhardt Henriksen Title: Research leader Investigator: Karen Eberhardt Henriksen Title: Research leader Discipline: P growth regulator (plant) Trial Status: F one-year/final Initiation Date: City: Slagelse State/Prov.: Sjælland Postal Code: 42 Country: DEN Denmark Trial Location Conducted Under GEP: X Guideline Description 1. EPPO Growth Regulation in Cerials Personnel Study Director: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Investigator: Karen Eberhardt Henriksen Title: Research leader Affiliation: Address: Forsøgsvej 1 Location: Slagelse Postal Code: 42 karene.henriksen@agrsci.dk Phone No.: Cooperator: Steen Sørensen Madegården I/S Address 1: Hindholmvej 24 Cooperator/Landowner Phone No.: City: Sandved State/Prov: Sjælland Mobile No.: Postal Code: 4262 Country: DEN Denmark Crop Description Crop 1: TRZAW Triticum aestivum (winter) Wheat Winter Variety: Ambition Description: CONVENTIONAL BBCH Scale: BCER Planting Date: Planting Method: DRILLE drilled Rate, Unit: 18 KG/HA Depth, Unit: 5 cm Perennial Age, Unit: 1 YEAR Row Spacing, Unit: 12,5 cm Seed Bed: MEDIUM medium Site and Design Plot Width, Unit: 2,5 m Site Type: FIELD field Plot Length, Unit: 5 m Experimental Unit: 28 PLOT plot Plot Area, Unit: 12,5 m2 Tillage Type: CONTIL conventional-till Replications: 4 Study Design: FACTOR Factorial % Slope:, Untreated Arrangement: INCLUDED single control randomized in each block Previous Crops Year 1. Common Corn Salad 28 Soil Description Description Name: Textureanalyze % Sand: 7,8 % OM: 2,4 Texture: GRSL gravelly sandy 53 loam

54 (245-1) Site Description Page 2 of 2 % Silt: 12,2 ph: 6,9 Soil Name: 5 % Clay: 14,6 CEC: 6,4 Fert. Level: G good Soil Drainage: G Analyzed By: AGROLAB GmbH Application Description Application Date: Time of Day: Application Method: A SPRAY Application Timing: Application Placement: BROFOL Applied By: Air Temperature, Unit: SCS % Relative Humidity: C Wind Velocity, Unit: 3 M/S Wind Direction: Dew Presence (Y/N): Soil Moisture: NE N no % Cloud Cover: 95 VD/D/M Crop Stage At Each Application Crop 1 Code, BBCH Scale: Stage Scale Used: A TRZAW BCER BBCH Stage Majority, Percent: 37 5 Stage Maximum, Percent: 38 5 Application Equipment Appl. Equipment: Equipment Type: Operating Pressure, Unit: 2.6 Nozzle Type: Nozzle Size: Nozzle Spacing, Unit: Nozzles/Row: 5 A Sprayer A SPLOVO Hardi Boom ID: V/1 Boom Length, Unit: Boom Height, Unit: Ground Speed, Unit: Carrier: ATM LD CM 2,5 M 5 CM 4,5 KPH Selfprop. Spray Volume, Unit: 15 L/HA Mix Size, Unit: 2 Liters Tank Mix (Y/N): N no 54

55 (245-1) AOV Means Table Page 1 of 4 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Pest Code Crop Code TRZAW TRZAW TRZAW TRZAW TRZAW Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type PHYGEN EARING PHYGEN LODGING HEIGHT Rating Unit PERCENT BBCH PERCENT PERCENT CM Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH Trt-Eval Interval 2 DA-A 2 DA-A 29 DA-A 29 DA-A 29 DA-A Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop a 55 a a a 8 b 2 Medax Top 1,5 L/ha Crop a 53 c a a 73 c 3 Moddus M,25 L/ha Crop a 55 a a a 85 a 4 Moddus M,5 L/ha Crop a 55 a a a 85 a 5 Terpal,75 L/ha Crop a 55 a a a 84 a 6 Terpal 1,5 L/ha Crop a 54 b a a 79 b 7 Untreated Check a 55 a a a 85 a LSD (P=.5),,4,, 2,2 CV,,48,, 1,78 Replicate F, 1,412,, 1,289 Replicate Prob(F) 1,,2719 1, 1,,386 Treatment F, 33,,, 4,362 Treatment Prob(F) 1,,1 1, 1,,1 Means followed by same letter do not significantly differ (P=.5, Student-Newman-Keuls) 55

56 (245-1) AOV Means Table Page 2 of 4 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) TRZAW TRZAW TRZAW TRZAW TRZAW PLANT C PLANT C PLANT C PLANT C EAR C HEIGHT PHYGEN LODGIN LOGDING WILTIN CM PERCENT PERCENT PERCENT PERCENT BBCH BBCH BBCH BBCH BBCH 51 DA-A 51 DA-A 51 DA-A 72 DA-A 72 DA-A 85 d a a a a 79 e a a a a 9 a a a a a 89 ab a a a a 88 bc a a a a 86 cd a a a a 91 a a a a a 2,,,,, 1,52,,,, 8,34,,,,,13 1, 1, 1, 1, 42,223,,,,,1 1, 1, 1, 1, 56

57 (245-1) AOV Means Table Page 3 of 4 Pest Code Crop Code Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Trt-Eval Interval Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage 1 Medax Top,75 L/ha Crop Medax Top 1,5 L/ha Crop Moddus M,25 L/ha Crop Moddus M,5 L/ha Crop Terpal,75 L/ha Crop Terpal 1,5 L/ha Crop Untreated Check LSD (P=.5) CV Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) TRZAW STRAW C WILTIN PERCENT 1 86 BBCH 72 DA-A a a a a a a a,,, 1,, 1, 57

58 (245-1) AOV Means Table Page 4 of 4 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code TRZAW, BCER, Triticum aestivum (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury EARING = Earing/Skridning HEIGHT = height LODGIN = lodging WILTIN = wilting Rating Unit PERCENT = percent BBCH = -99 BBCH scale CM = centimeter Crop Stage Majority 55 = Middle of heading: half of inflorescence emerged 65 = Full flowering: 5% of anthers mature 73 = Early milk Crop Stage Scale BBCH = BBCH uniform plant stages 58

59 (245-1) Assessment Data Summary Page 1 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Crop Name Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type PHYGEN EARING PHYGEN LODGING HEIGHT HEIGHT PHYGEN LODGIN Rating Unit PERCENT BBCH PERCENT PERCENT CM CM PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI VLI VLI VLI VLI VLI VLI VLI Days After First/Last Applic Trt-Eval Interval 2 DA-A 2 DA-A 29 DA-A 29 DA-A 29 DA-A 51 DA-A 51 DA-A 51 DA-A Plant-Eval Interval 262 DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = Medax Top 1,5 L/ha Crop Mean = Moddus M,25 L/ha Crop Mean = Moddus M,5 L/ha Crop Mean = Terpal,75 L/ha Crop Mean = Terpal 1,5 L/ha Crop Mean =

60 (245-1) Assessment Data Summary Page 2 of 5 Crop Code TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW TRZAW BBCH Scale BCER BCER BCER BCER BCER BCER BCER BCER Crop Scientific Name Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Crop Name Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Winter wheat Part Rated PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C PLANT C Rating Date Rating Type PHYGEN EARING PHYGEN LODGING HEIGHT HEIGHT PHYGEN LODGIN Rating Unit PERCENT BBCH PERCENT PERCENT CM CM PERCENT PERCENT Number of Subsamples Crop Stage Majority Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Footnote Number Assessed By VLI VLI VLI VLI VLI VLI VLI VLI Days After First/Last Applic Trt-Eval Interval 2 DA-A 2 DA-A 29 DA-A 29 DA-A 29 DA-A 51 DA-A 51 DA-A 51 DA-A Plant-Eval Interval 262 DP DP DP DP DP DP DP DP-1 Number of Decimals Trt Treatment Rate Growth No. Name Rate Unit Stage Plot 7 Untreated Check Mean =

61 (245-1) Assessment Data Summary Page 3 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth TRZAW TRZAW TRZAW BCER BCER BCER Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Winter wheat Winter wheat Winter wheat PLANT C EAR C STRAW C LOGDING WILTIN WILTIN PERCENT PERCENT PERCENT BBCH BBCH BBCH HEJE HEJE HEJE DA-A 72 DA-A 72 DA-A 314 DP DP DP-1 No. Name Rate Unit Stage Plot 1 Medax Top,75 L/ha Crop Mean = 2 Medax Top 1,5 L/ha Crop Mean = 3 Moddus M,25 L/ha Crop Mean = 4 Moddus M,5 L/ha Crop Mean = 5 Terpal,75 L/ha Crop Mean = 6 Terpal 1,5 L/ha Crop Mean = 61

62 (245-1) Assessment Data Summary Page 4 of 5 Crop Code BBCH Scale Crop Scientific Name Crop Name Part Rated Rating Date Rating Type Rating Unit Number of Subsamples Crop Stage Majority Crop Stage Scale Footnote Number Assessed By Days After First/Last Applic. Trt-Eval Interval Plant-Eval Interval Number of Decimals Trt Treatment Rate Growth TRZAW TRZAW TRZAW BCER BCER BCER Triticum aestivum (winter) Triticum aestivum (winter) Triticum aestivum (winter) Winter wheat Winter wheat Winter wheat PLANT C EAR C STRAW C LOGDING WILTIN WILTIN PERCENT PERCENT PERCENT BBCH BBCH BBCH HEJE HEJE HEJE DA-A 72 DA-A 72 DA-A 314 DP DP DP-1 No. Name Rate Unit Stage Plot 7 Untreated Check Mean = 62

63 (245-1) Assessment Data Summary Page 5 of 5 Growth regulation in winter cereals - Efficcacy Trial ID: Protocol ID: 245 Location: DK Study Director: Karen Eberhardt Henriksen Project ID: R-86 Investigator: Karen Eberhardt Henriksen Sponsor Contact: BASF Crop Code TRZAW, BCER, Triticum aestivum (winter), = IE Part Rated PLANT = plant EAR = ear STRAW = straw C = Crop is Part Rated Rating Type PHYGEN = phytotoxicity - general / injury EARING = Earing/Skridning HEIGHT = height LODGIN = lodging WILTIN = wilting Rating Unit PERCENT = percent BBCH = -99 BBCH scale CM = centimeter Crop Stage Scale BBCH = BBCH uniform plant stages Assessed By VLI = Verner Lindberg HEJE = Henrik Jespersen Plant-Eval Interval 262 DP-1 = DP-1 = DP-1 = DP-1 = Footnote 1: Den 2. juni 2: Verner Footnote 2: Den 11. juni 2: Steen og Verner Footnote 3: Den 3 juli 2: Steen og Verner Footnote 4: 24/7- Henrik 63

64 Faculty of Agricultural Sciences, Department of Integrated Pest Management Appendix A. The following pages in appendix A, is printed from our database of accumulated data. Research Centre Flakkebjerg Flakkebjerg 64 Tel: DK-42 Slagelse Fax:

65 Faculty of Agricultural Sciences, Department of Integrated Pest Management 615 Medax Top (,375=afg_st.37-39) Medax Top,375 L/ha. 1. Spraying Crop BBCH Barley, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Regrowth, New stemes (%) Height reduction (%) Grain golden Phytotoxicity - General injury (%) Loading (=no loading, 1=all loading) Grain golden 3 weeks after Grain golden Wilting of spike (%) 3 weeks after Grain golden Wilting of stem (%) 3 weeks after Grain golden Wheat, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Height reduction (%) Earing completed Phytotoxicity - General injury (%) Earing completed Loading (=no loading, 1=all loading) Earing completed Grain golden Wilting of spike (%) Wilting of stem (%) 6151 Medax Top (,75=afg_st.37-39) Medax Top,75 L/ha. 1. Spraying Crop BBCH Barley, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) at Earing Earing completed Regrowth, New stemes (%) november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 1 of 6

66 2 Height reduction (%) Earing completed Grain golden Phytotoxicity - General injury (%) Earing completed 3 3 Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) Grain golden 3 weeks after Grain golden Wilting of stem (%) Grain golden weeks after Grain golden Wheat, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) Earing completed Regrowth, New stemes (%) 2 Height reduction (%) Earing completed Grain golden Phytotoxicity - General injury (%) Earing completed Grain golden 4 4 Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) 3 weeks after Grain golden 3 3 Wilting of stem (%) 3 weeks after Grain golden 3 3 Rye, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) at Earing november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 2 of 6

67 Height reduction (%) Earing completed Phytotoxicity - General injury (%) Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) Wilting of stem (%) 1 2 Triticale, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Height reduction (%) Grain golden Phytotoxicity - General injury (%) Earing completed Loading (=no loading, 1=all loading) Earing completed Grain golden Wilting of spike (%) 3 weeks after Grain golden Wilting of stem (%) 3 weeks after Grain golden 6152 Medax Top (1,5=afg_st.37-39) Medax Top 1,5 L/ha. 1. Spraying Crop BBCH Barley, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) at Earing Earing completed Regrowth, New stemes (%) 2 Height reduction (%) Earing completed Grain golden Phytotoxicity - General injury (%) Earing completed 3 3 Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 3 of 6

68 Wilting of spike (%) Grain golden 3 weeks after Grain golden Wilting of stem (%) Grain golden weeks after Grain golden Wheat, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) Earing completed Regrowth, New stemes (%) 2 Height reduction (%) Earing completed Grain golden Phytotoxicity - General injury (%) Earing completed Grain golden Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) 3 weeks after Grain golden 3 3 Wilting of stem (%) 3 weeks after Grain golden 3 3 Rye, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) at Earing 1-4 Height reduction (%) Earing completed Phytotoxicity - General injury (%) Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 4 of 6

69 Wilting of stem (%) 1 2 Triticale, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Height reduction (%) Grain golden Phytotoxicity - General injury (%) Earing completed Loading (=no loading, 1=all loading) Earing completed Grain golden Wilting of spike (%) 3 weeks after Grain golden Wilting of stem (%) 3 weeks after Grain golden 6153 Medax Top (3,=afg_st.37-39) Medax Top 3 L/ha. 1. Spraying Crop BBCH Barley, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) Earing completed 1-3 Regrowth, New stemes (%) Height reduction (%) Earing completed Phytotoxicity - General injury (%) Earing completed 2 Loading (=no loading, 1=all loading) Earing completed Grain golden Wilting of spike (%) Grain golden Wilting of stem (%) Grain golden Wheat, Winter Phytotoxicity - Visual Assessment time Undersowed crop: Number obs. Average Standard Dev. Crop stage deviation (from untreated) Earing completed 1-2 Regrowth, New stemes (%) Height reduction (%) Earing completed november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 5 of 6

70 Phytotoxicity - General injury (%) Earing completed Loading (=no loading, 1=all loading) Earing completed Grain golden 3 weeks after Grain golden Wilting of spike (%) 3 weeks after Grain golden Wilting of stem (%) 3 weeks after Grain golden november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 6 of 6

71 Faculty of Agricultural Sciences, Department of Integrated Pest Management 615 Medax Top (,375=afg_st.37-39) Medax Top,375 L/ha. 1. Spraying Crop BBCH 6151 Medax Top (,75=afg_st.37-39) Medax Top,75 L/ha. 1. Spraying Crop BBCH 6152 Barley, Winter Surplus. Grain (hkg/ha 15%) Wheat, Winter Yield compared to Untreated Number obs. Average Standard dev. Untreated 2 2,4 3 2(85)23 Yield compared to Untreated Number obs. Average Standard dev. Untreated Surplus. Grain (hkg/ha 15%) 2-1,5 2(114)2 Medax Top (1,5=afg_st.37-39) Medax Top 1,5 L/ha. 1. Spraying Crop BBCH 6153 Barley, Winter Surplus. Grain (hkg/ha 15%) Wheat, Winter Yield compared to Untreated Number obs. Average Standard dev. Untreated 2 -,15 1 2(85)23 Yield compared to Untreated Number obs. Average Standard dev. Untreated Surplus. Grain (hkg/ha 15%) 2-3,4 2(114)2 Medax Top (3,=afg_st.37-39) Medax Top 3 L/ha. 1. Spraying Crop BBCH Barley, Winter Surplus. Grain (hkg/ha 15%) Wheat, Winter Surplus. Grain (hkg/ha 15%) Yield compared to Untreated Number obs. Average Standard dev. Untreated 2 3,2 7 2(85)23 Yield compared to Untreated Number obs. Average Standard dev. Untreated 2-7,45 3 2(114) november 2 Research Centre Flakkebjerg, DK-42 Slagelse Phone: Page 1 of 1

72 Faculty of Agricultural Sciences, Department of Integrated Pest Management Appendix B. 1. Reading Guide Guide Lines EPPO Guidelines are used as basis for the trial work at the Faculty of Agricultural Sciences, Department of Integrated Pest Management (Effect of Pesticide). At a number of situations the EPPO Guidelines give a possibility to choose between several methods. Therefore, it is the methods used by the Faculty of Agricultural Sciences, Department of Integrated Pest Management (Effect of Pesticide) that are described in the following. Trial Design All trials have been carried out as field trials at farmers in Zealand and Jutland. They are designed as randomised bloc design with 2 factors, herbicides and doses, with untreated as comparison treatment. Three different designs are normally used: 1. Selectivity trial with normal dose and double dose, respectively (1/1 and 2/1). 2. Efficacy trial, 3 doses with quarter, half and normal dose, respectively (1/4, 1/2 and 1/1). 3. Efficacy trial, 2 doses with half and normal dose, respectively (1/2 and 1/1). Growth regulation trials are normally with half, normal and double dose, respectively (1/2, 1/1 and 2/1). Desiccation trials are normally with half and normal dose, respectively (1/2 and 1/1). Dosage of surfactant and oil follows the water volume and not the dosage. Therefore, the quantity of surfactant and oil is the same irrespective of herbicide dosage. Trial descriptions Estimations of climatic and soil characteristics at the time of treatment: Cloud cover: Soil moisture: Clear sun Misty sun Slightly clouded sun Slightly clouded Overcast Very dry Dry Moist Wet Very wet Research Centre Flakkebjerg Flakkebjerg 72 Tel: DK-42 Slagelse Fax:

73 Faculty of Agricultural Sciences, Department of Integrated Pest Management Water balance of the plants: Wet Slightly damp Dry, turgid Dry Marked by drought Visual Assessments and Scales At the estimation of stage of development of crop and weed, the BBCH scales are used if these are elaborated. In 25 the BBCH scales are used for cereals, oil seed rape, beets, maize, peas and potatoes. For other crops and for weeds, the BBCH common scales are used. Scorings on crop: Non specified: Scale (percentage): ARM Code Crop damage (phytotoxicity) - 1. untreated = PHYGEN Specified: Leaf/ear/stem deformity - 1. untreated = PHYDEF (Part of total foliage/ear/stem with abnormal shape) Leaf colour - 1. untreated = - Bleaching PHYBLE - Chlorosis PHYCHL (Part of total foliage with diverging colour) Leaf necroses - 1. untreated = PHYNEC (Part of total foliage dead) Mortality - 1. untreated = MORTAL (Part of plants missing) Lodged corn - 1. LODGIN (All parcels) Research Centre Flakkebjerg Flakkebjerg 73 Tel: DK-42 Slagelse Fax:

74 Faculty of Agricultural Sciences, Department of Integrated Pest Management Evaluation of the effects on weeds All visual assessment of effect on weeds, are carried out in following way. The untreated plots are estimated for coverage by using scale -1 %. The effect is seen as effect in relation to biomass in untreated plots by using scale -1 %. Coverage in untreated plots 1 % Effect in treated plots 1 % The calculations of effect to the individual weed species are carried out by a registration of numbers and fresh weight of each species after three counts of.25 m² (or.1 m²) in all plots. The results are stated as average of 3 counts and 4 replications and as percent effect compared to the untreated plots. Weed species, which are estimated as too sparse or unequally represented, are summarised DICSP. All analyses of variance are made on original data. Research Centre Flakkebjerg Flakkebjerg 74 Tel: DK-42 Slagelse Fax:

75 Faculty of Agricultural Sciences, Department of Integrated Pest Management 2. The Climate in Flakkebjerg For the country in general the climate during the growing season (Sept. 28-Aug. 2) was mild and sunny. The average temperature was 1,2 C above normal and the global radiation was 12% above normal. The precipitation was 3% less than normal and the evaporation was 16% higher than normal. At Flakkebjerg the autumn (September November) was sunny and the average temperature was normal. September was exceptional dry but the rainfall during October November was a bit higher than normal. The first frost came late (1. November). The weather did not affect the sowing of winter crops. The winter (December February) was cold and dry. Especially January was very dry with only 5% of the normal precipitation. The spring (March May) was warm and sunny and in April the rainfall was only 6mm. The evaporation was much higher than normal, and some crops were affected by the dry and sunny weather. The summer (June August) was warm and sunny. June was wet but it was owing to heavy rainfall (66 mm) on the June. August was very dry (16 mm). The harvest started 2 weeks before normal. Due to the rain in May and June the yields were normal. The automatic weather station at Flakkebjerg is located 12 km from the West Zealand coast. The climate at Flakkebjerg is representative of the area in which most of our trials are situated. The normal climate is given as an average of thirty years ( ). Research Centre Flakkebjerg Flakkebjerg 75 Tel: DK-42 Slagelse Fax:

76 Faculty of Agricultural Sciences, Department of Integrated Pest Management Temperature Year Temperature Normal Global radiation Year Global radiation Normal 2, 18, 16, 14, 12, 1, 8, 6, 4, 2,, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- 7, 6, 5, 4, 3, 2, 1,, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- Precipitation Year Precipitation Normal Water balance Year Water balance Normal 9, 6, 8, 4, 7, 2, 6,, 5, 4, 3, 2, 1,, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- -2, -4, -6, -8, -1, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- Figure 1: Climate data from Research Centre Flakkebjerg for growing season September 8- August. The temperature is in C, the global radiation is measured in MJ/m 2, the precipitation in mm and the water balance is the difference between precipitation and potential evaporation. Research Centre Flakkebjerg Flakkebjerg 76 Tel: DK-42 Slagelse Fax:

77 Faculty of Agricultural Sciences, Department of Integrated Pest Management Temperature - Jyndevad 28/2 Global Radiation - Jyndevad 28/2 18, 7, 16, 6, Grader C 14, 12, 1, 8, MJ/m2 5, 4, 3, 6, 4, 2, 2, 1,, sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug-, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- Precipitation - Jyndevad 28/2 Water balance - Jyndevad 28/2 2 2, , 14 1, mm 12 1 mm 5, 8, 6 4-5, 2 sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug- -1, sep- 8 okt- 8 nov- 8 dec- 8 jan- feb- mar- apr- maj- jun- jul- aug- Figure 2: Climate data from the automatic weather station located at Jyndevad and representative of our trials in Southern Jutland. Research Centre Flakkebjerg Flakkebjerg 77 Tel: DK-42 Slagelse Fax:

78 Faculty of Agricultural Sciences, Department of Integrated Pest Management Temperature - Bygholm 28/2 Global Radiation - Bygholm 28/ Grader C MJ/m sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug- sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug- Precipitation - Bygholm 28/2 Water balance - Bygholm 28/2 8 6, 7 4, 6 2, 5, mm sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug- mm -2, -4, -6, -8, -1, sep-8 okt-8 nov-8 dec-8 jan- feb- mar- apr- maj- jun- jul- aug- Figure 3: Climate data from the automatic weather station located at Bygholm and representative of our trials in the central part of Eastern Jutland. Research Centre Flakkebjerg Flakkebjerg 78 Tel: DK-42 Slagelse Fax:

79 Faculty of Agricultural Sciences, Department of Integrated Pest Management April May June July August April - August Figure 5: Water balance in the growing season 2. Research Centre Flakkebjerg Flakkebjerg 79 Tel: DK-42 Slagelse Fax:

80 Faculty of Agricultural Sciences, Department of Integrated Pest Management 3. Extract from»general provisions for commissioned assignments«september Liability 7.1.The Faculty of Agricultural Sciences is liable towards the giver of the assignment in accordance with the rules of liability for damages in Danish law, with the limitations resulting from clauses , cf. also clause The work, reports, opinions and instructions supplied by the Faculty of Agricultural Sciences have taken place and been made on the basis of the knowledge and technique that the Faculty of Agricultural Sciences has at its disposal at the time of the implementation of the work. The Faculty of Agricultural Sciences shall be without any liability whatsoever if a later development should prove the knowledge and technique of the Faculty of Agricultural Sciences to have been defective or incorrect. This same applies to opinions of which it has been stated that they are based on an estimated judgement or evaluation. 7.3.If the assignment consists in the preparation of edp-programmes, or if calculations, results or other statements are based on the application of edp, the Faculty of Agricultural Sciences shall be without liability for any damage that may be a consequence of errors in the developed or applied programmes. 7.4.The Faculty of Agricultural Sciences shall be without liability for any damage that may occur in connection with any type of use which is outside the assignment or outside the purpose in connection with which the services of the Faculty of Agricultural Sciences were rendered In relation to a third party, the giver of the assignment shall have the full liability for any damage that may be caused by products manufactured by the giver of the assignment in applying the results of the assignment. The Faculty of Agricultural Sciences shall have the liability for any damage solely to the extent that the giver of the assignment can substantiate that the damage caused can be traced back to a negligent act on the part of the Faculty of Agricultural Sciences. If staff employed by the giver of the assignment assist at the performance of an assignment, the Faculty of Agricultural Sciences shall be without any liability whatsoever for faults or deficiencies which could be traced back to work carried out by such staff. 7.6.The liability of the Faculty of Agricultural Sciences can - unless something else has explicitly been agreed upon - never exceed DKK 5, for each occurrence. A number of occurrences involving a loss shall be regarded as one single occurrence if the cause can be traced back to the same fault or deficiency. The Faculty of Agricultural Sciences will never be liable for loss of production, consequential loss, loss of profits or any other indirect loss. The Faculty of Agricultural Sciences cannot be held responsible for damage that has not been notified in writing within five years after the Faculty of Agricultural Sciences has rendered the service on which the liability is based. The general rules of Danish law on limitation shall apply irrespective of whether objections have been made to the Faculty of Agricultural Sciences To the extent that a liability extending beyond the limits stipulated in clauses should be imposed on the Faculty of Agricultural Sciences in relation to a third party, the giver of the assignment shall be under an obligation to indemnify the Faculty of Agricultural Sciences for this. Research Centre Flakkebjerg Flakkebjerg 8 Tel: DK-42 Slagelse Fax:

81 Faculty of Agricultural Sciences, Department of Integrated Pest Management 4. GEP Certificate. Research Centre Flakkebjerg Flakkebjerg 81 Tel: DK-42 Slagelse Fax: