N-Fix Technology New delivery system for Nitrogen. Peter Blezard CEO/Founder

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1 N-Fix Technology New delivery system for Nitrogen Peter Blezard CEO/Founder 1

2 Nitrogen Pollution Issues Dead zones Water contamination Greenhouse gases

3 Agriculture WILL Change 3

4 Climate Smart Agriculture Climate smart agriculture needs natural nitrogen fixation - farming practices must be more adaptive to environmental pressures N-Fix is an environmentally friendly seed coating which increases crop yields - contributes to healthier food 4

5 Our Vision The nitrogen industry is worth approx. $110bn annually. We're going to revolutionise this industry. We're going to change the way that people grow food. What we have created will affect every single person on the planet now AND our future generations. We have created the single most important breakthrough in agriculture in the last 100 years. Our science will change the world. 58

6 Azotic Technologies Disruptive Technology Sustainable nitrogen to transform agriculture A global solution to improve crop performance and help reduce nitrogen pollution Reduces Nitrogen fertiliser need thereby drastically reducing farming costs Will improve the quality of water, soil and the atmosphere Rice & corn field trials have shown positive yield increases Azotic is an intellectual company with a commercial road map a BioScience/Life Science - Cleantech sector company, focusing on social farming and the agronomic future 6

7 Nitrogen-fixing Bacteria Intra-cellular colonisation of a nitrogen fixing bacteria - an unprecedented opportunity! A published patent and a subsequent 2006 scientific publication described the impact of the genomic and enzymatic traits of a unique strain of Gluconacetobacter diazotrophicus (Gd) Gd is a dose dependent delivery system, it uses the root meristematic cell target sites and its potential generic use for global crops Provenance of approach and technology based on: Ø Father of Green Revolution Nobel Peace Prize - Norman Borlaug Ø Founding Father of Modern Plant Biotechnology - Prof Ted Cocking FRS

8 N-Fix Technology Is the only proven technology that enables all crop species to fix nitrogen directly from air Naturally occurring, Nitrogen fixing bacteria from sugarcane - takes up and uses Nitrogen from the air An extraordinary endophytic, diazotophic, acid tolerant bacteria promotes Indole Acetic Acid (IAA) which promotes plant growth In intracellular state the bacteria forms a symbiosome within the plant cell Gd is a harmless bacteria, contains no toxins and is neither genetic modification (GM) nor bio-engineering Applied to the cells of plants via the seed - provides every cell in the plant with the ability to fix Nitrogen Enables naturally sustainable farming without nitrogen pollution 8

9 N-Fix - How it works 9

10 N-Fix Pathway Azotic IP Patent Benefit Preloaded seeds with Nitrogen the power behind crop nutrition and health

11 Azotic IP Protection & valuable scale-up know how Azotic IP Patents Knowhow Ø Ø Ø Ø Ø Ø Description Azotic has been exclusively licensed for certain patents owned by the University of Nottingham. These relate to the activity of GD in plants and the mode-of-action with regard to the entry Azotic has also filed and been awarded a number of subsequent patents These relate to follow-on discoveries of GD or niche market applications, such as the method of inoculation Azotic has significant, non-patent, know-how that contributes to the success of the company Among this, is information obtained through research (what was tried and did not work, lab setup and manufacturing processes), technical trade secrets, and invention disclosures not-yet-filed in patent applications Azotic has gathered a significant amount of data on the bacteria and possible supporting agents which would need a considerable time to replicate Patent Region Expiry UoN intercellular & method of inoculation Terribacillus beneficial agent Wound mature crops & novel formulations Specific strain distinct genetic sequence Diagnostic test identifying specific strain and farmer tool Granted- Europe, USA Canada (pending) PCT Europe, Canada, USA PCT Numerous countries PCT Numerous countries PCT Europe, Canada, USA August 2022 July 2035 July 2035 July 2036 July 2036 The company has investigated any patents that might restrict freedom-to-operate Though there are several patents that relate to the application of GD for agricultural uses, they do not see any infringement in their freedom-to-operate 11

12 Azotic Products Portfolio of Products New delivery systems for agriculture: Liquid Coating In Seed Spray 12

13 Product Development Powder formulations Powdered seed treatments with dried Gd for on-seed application Foliar spray Bioinformatics analysis to guide formulation development Re-annotating the genome sequence Constructing metabolic network from re-annotated genome This disruptive technology is the most important agricultural breakthrough in the last 100 years - It will revolutionise agriculture 13

14 Investment: Technology Value Proposition Unique Gd Strain Biomass Production Process & Tech Transfer Package Formulated Product Concentrated, Effective & Stable Enforceable IP Barriers to entry Grower/Field trial data Business case: Input cost vs yield improvement 14 14

15 Field Trials Maize Maize Maize Maize Rice Corn Rice Corn Maize 12

16 Field Trial Results Corn trial carried out in Dana, Iowa showed that N-Fix-treated plots increased yield by 50% (2.7 t/ha) when no fertiliser N was applied and 15% (1.6 t/ha) when the full recommend fertiliser N was applied. Soybean trial in Dana, Iowa showed highly significantly yield increases in N-Fix treatment compared to control at two N levels - 29% (840 kg/ha) at 0 N and 8.4% (300 kg/ha) at 100 N (2017). Four spring wheat trials in the UK, Germany and the US showed that overall N-Fix increased yield by 7% (460 kg/ha) and also demonstrated a potential to N-fertiliser savings of up to 61% with no reduction in yield. In three Vietnamese rice trials the overall response was a mean average 20% (825 kg/ha) yield increase across all the field trials (2017). Two rice trials were carried in Thailand and two in the Philippines. Overall on the N-Fix treated plots yields were significantly higher than the untreated at every fertiliser N level tested - the N-Fix treated plots yielded 17% (645 kg/ha) more than the untreated, with a 1 t/ha (29%) increase being seen at 50% of the recommended fertiliser N (2018). 16

17 Thank You Peter Blezard CEO Azotic Technologies Ltd ltd t:+ 44 (0) Azotic is the future in sustainable nitrogen 17