Principal Investigator Dr. S.R. VOLETI

Transcription

1 ANNUAL PERSONAL PROGRESS REPORT On NATIONAL INITIATIVE ON CLIMATE RESILIENT AGRICULTURE ( ) Principal Investigator Dr. S.R. VOLETI Co PIs: Dr. N. SHOBHA RANI Dr. V.P BHADANA Dr. L.V. SUBBA RAO PRESENTED BY Kumaraswamy Manthri Senior Research Fellow, Plant Breeding Section, Directorate of Rice Research.

2 Introduction : OBJECTIVES : Rice is the world s second-most produced staple crop and it has been estimated that 90 % of the rice crop grown mainly in Asian Countries. Global warming is a serious peril to the rice production. High temperatures ( C) during anthesis stage of flowering induce spikelet sterility which in turn decreases the rice yield. Currently, very few donors lines for heat stress tolerance are identified from the rice germplasm collection present at the IRRI Gene bank. The rice variety N22 is the only donor so far identified which is showing promising tolerance to heat stress. To screen wide range of rice genotypes (IRHTN 2009 and CPAU) for heat stress tolerance and understand its effect on various agro-morphological characters. To identify suitable heat tolerant donors to be used for developing high yielding heat tolerant varieties. To develop breeding populations using identified donors to develop high yielding heat tolerant varieties confined with other desirable traits that is plant type etc,

3 Summary of Work done during Rabi 2012 Screening of Heat Tolerant Lines during Rabi 2012 As per the proposed work plan under NICRA a diverse set of genotypes comprising IRHTN 2009, CPAU was screened for heat tolerance and other attributes during Rabi To ensure the exposure of the maximum lines to high temperature > 40 0 C at reproductive stage sowing was done in one staggers List of the plant materials screened at Ramachandrapuram ICRISAT farm during Rabi 2012 S.NO Particulars No of Genotypes 2 4 IRHTN 2009(1sets) 33 CPAU (1set) 30 Total 63 List of observations recorded. 1. Days to 50% flowering 2. Plant height (CM) 3. Number of Productive tillers 4. Spikelet fertility 5. Panicle length 6. Number of grains per panicle 7. Percent of chalkiness in grain (%) 8. Test weight (Gms)

4 Objectives achieved Observations recorded and spikelet fertility Summarized below table S.NO Materia l No of plants observed at the temperature highest temperature during Rabi 2012 A (>90%) B (>75% ) C (>60%) D (<60%) E (<40%) 1 IRHTN CPAU Total accessions Note: A Highly tolerant, B Tolerant, C Slightly tolerant, D- Susceptible, E Highly susceptible Spikelet fertility of IRHTN 2009 and CPAU genotypes at 40 0 C during Rabi 2012 IRHTN 2009 Genotypes in IRHTN 2009 recorded more than 90% fertility at 40 0 C were IRHTN ( N 22), IRHTN 110 (GIZA 176), IRHTN 128 (DULAR (ACC 32561)), IRHTN 129 (IR 20), IRHTN 131 (IR 1552), IRHTN 133 (CR ) CPAU The considerable variability was observed among all 30 genotypes under heat screening mainly focused for spikelet fertility at high temperatures (>40 o C) the genotypes CPAU 3, CPAU 9, CPAU 10, CPAU 11, CPAU 14, CPAU 16, CPAU 18, CPAU 22 and CPAU 28 showed >90% spikelet fertility and CPAU 7 showed low spikelet fertility < 70%

5 Graph 2: Depicting Spikelet fertility at day wise temperatures of CPAU during Rabi /1/2012 4/10/2012 4/10/2012 4/18/2012 4/10/2012 4/18/2012 4/23/2012 5/1/2012 4/20/2012 4/18/2012 CPAU 3 CPAU 9 CPAU 10 CPAU 11 CPAU 14 CPAU 16 CPAU 18 CPAU 22 CPAU 28 CPAU 7 TEMP SP FRT Graph 1: Depicting Spikelet fertility at day wise temperatures of IRHTN 2009 during Rabi /17/2012 4/14/2012 4/3/2012 4/30/2012 4/30/2012 TEMP SP FRT IRHTN N 22 IRHTN GIZA 176 IRHTN DULAR (ACC 32561) IRHTN IR 20 IRHTN CR

6 Breeding material during Rabi 2012 Table3:Following material were sown at Ramachandrapuram,ICRISAT farm during the kharif 2012 session and selected SPS given below table S.No. Generation No of SPS 1 F F F3 231 Breeding material Selections duringkarif2012 Following material were sown at Ramachandrapuram, ICRISAT farm during the kharif 2012 session and selected SPS given below table S.No. Generation No of SPS 1 F F F4 593

7 Crossing programme during Kharif 2012 During Khari 2012 fallowing several crosses were made Based on Rabi 2012 screening and also literature reports few donors were identified and following crosses were attempteda Total of 42 crosses were made during Kharif 2012 to generate breeding material for heat tolerance the details are given below; S. No. Cross combinations 1 IRHTN IRHTN EC-455 IRHTN IRHTN IRHTN HTL-12 IRHTN EC-438 CPAU-9 6 IRHTN IRHTN HTL-7 IRHTN IRHTN IRHTN IRHTN IRHTN IRHTN IRHTN HTL-6 IRHTN IRHTN EC IRHTN EC CPAU-9 IRHTN HTL-6 IRHTN HTL-6 IRHTN IRHTN IRHTN IRHTN IRHTN IRHTN IRHTN IRHTN Rp-Bio HTL-12 MTU IRHTN IR HTL-12 Rp-Bio EC-441 Swarana 25 IRHTN Sampada 26 VANDANA IRHTN VANDANA EC IRHTN CPAU 7 30 EC 451 IRHTN EC 455 IRHTN HTL 6 IRHTN EC 450 IRHTN IRHTN HTL 6 35 IRHTN EC IRHTN IRHTN IRHTN IRHTN Sugandhamati Swarana 39 EC 436 EC SUGANDAMATI EC EC 450 EC SUGANDAMATI IRHTN

8 Wild rice sowing and planting and leaf collection at ICRISAT farm Sowing and planting and leaf collection A total of 59 wild rice accessions belonging to eleven species and 228 wild rice belonging to four species were obtained from Punjab Agricultural University (PAU), Ludhiana and IGAU Raipur. Other activities Helped in departmental breeding activities nearly 24 DUS characterization taken of nearly 550 basmati entries.

9 Work Plan Rabi and Kharif ( ) S. No Material No of sets planted No of genotypes in 1 set 1 International Rice Heat Tolerant Nursery (IRHTN) IRHTN 2012 IRHTN 2011 IRHTN 2010 IRHTN sets 3 sets 3 sets 3 sets 2 EC & NERICAs 3 sets 67 3 CPAU 3 sets 30 4 HTL 3 sets 15 Total 301 The selected material sown at three staggered sowing and transplanting between each staggered sowing will be differed by at least days. Main screening and selection criteria would be based on spikelet fertility as well as yield components. The selected genotypes from yield &performance would further analyzed at molecular level using SSR markers particular to heat tolerance

10 SL NO QTL NAME ON CHOROSOME MARKER 1 Qhr1 1 RG381-RZ19 2 qhr3-1 3 RZ892-RG100 3 qhr4-3 4 RG214-RG143 4 qhr8-1 8 RG978-RG1 5 qhr RZ400-RG118 6 qhr RG118-Adh 7 qht3 3 RM570-RM148 8 qht9a 9 RM108-RM242 9 qht12 12 MRG0103- RM qht2 2 RM183-RM qht3 3 RM570-RM qht8 8 RM547- MRG qht9b 9 RM242-RM qhts-2 2 RM qhts-3 3 RM157b qhts-5 5 RM RM RM SSPf4 4 RM5687-RM SPf10 10 RM6132- RM6100 Poster presentation: HEAT QTL REPOTED QTL AND ON CHOMOSOME AND ITS PRIMERS SL NO 21 QTL NAME ON MARKER CHOROSOME SSPc4 4 RM5687-RM471 SSPc10 10 RM RM qtl_2.3 2 RG qtl_4.1 4 C qtl_1.1 1 B1065E10 26 qtl_2.2 2 C qtl_8.2 8 G qtl_1.1 1 B1065E10 29 qtl_8.1 8 RIM2A 30 qtl_ C16 31 qtl_1.1 1 B1065E10 32 qtl_3.4 3 R qtl_8.3 8 RG qtl_ C16 35 qtl_1.1 1 B1065E10 36 qtl_ G qtl_ C16 38 qtl_1.1 1 B1065E10 39 qtl_ C16 40 qtl_ G1465 IDENTIFICATION OF DONORS FOR HEAT TOLERANT IN RICE

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