ROLAND V. RALLOS* Agriculture Research Section Philippine Nuclear Research Institute

Transcription

1 Influene of Potssium Soluilizing Bteri on Growth nd Rdioesium Aumultion of Brssi rp L. vr. perviridis grown in Cs-Contminted Fukushim Soils ROLAND V. RALLOS* Agriulture Reserh Setion Philippine Nuler Reserh Institute A study onduted under the supervision of Dr. T. Yokoym during the FNCA-MEXT Fellowship Progrm (Otoer 2013-Mrh 2014) Tokyo University of Agriulture nd Tehnology, Jpn Tehnil Workshop on Remedition of Rdiotive Contmintion in Agriulture" Otoer 2016 Vienn, Austri.

2 Agriulture in Fukushim These three prefetures were seriously dmged in the Gret Est Jpn Erthquke on 11, Mrh, ommeril frms in Fukushim re 70,520 2nd mong 47 prefetures in Jpn Yokoym, 2013

3 Limited griulturl knowledge to find the est wy to ope with the diffiult situtions on Mrh Deep plowing go.jp/index.html Removl of surfe soil The deep plowing nd removl of surfe soil re very effetive to redue ontmintion of rdiotive Cs from ontminted soils. Deep plowing: 50% redution of rdiotive Cs in surfe soil. Removl of surfe soil : 75% redution of rdiotive Cs from ontminted soil. Mny reserhers elonging to different universities me together to help solve the prolem. Yokoym, 2013

4 Development of ioremedition of rdiotive Cs from ontminted soils using plnt-miroe intertion - Growth enhnement (root growth & tivity), whih leds to mximum rdiotive Cs uptke to the plnt Yokoym, 2013 Plnt Growth Promoting Rhizoteri - Serh for potssium soluilizing teri, whih produe idi mterils nd those n open the inner-sphere of ly minerls nd relese Cs Ctions suh s N +, C 2+ K + neutrlize the negtive hrge Cs + fits to the hole nd re strongly ound (Delvux et l., 2001) Enles effetive removl of movle (moile) Cs from the soil!

5 Preliminry results in TUAT Brssi rp vr. perviridis (Komtsun) Whole lyer 5m surfe 5m ottom Removl rte Billus PGPR : 5% of Cs Azospirillum PGPR: 9% of Cs Non-inoulted, Bilus, Azospirillum (TUAT, 30 th Nov. 2011) (Slem et l. 2013) Trnsfer ftor = (Bq kg -1 plnt) / (Bq kg -1 kg soil) Yokoym, 2013

6 The Experiment Explortion of KSB nd its influene on the growth nd rdioesium umultion in Komtsun

7 Priniples mnipultions of the growth medi ioni onentrtions nd rtios (e.g. K + : Cs + ) inresing plnt iomss in the ontminted soils per unit re pgpr s n inrese plnt iomss nd uptke of metls nd rdionulides prtil pplition to mnge or if possile enhned phytotrnsfer in ontminted soils.

8 Potssium soluilizing teri (KSB) rhizospheri miroorgnism whih usully pplied s hep iofertilizer to soluilize the insolule potssium (K) to solule forms of K + min mehnism of KSB is idolysis, heltion, exhnge retions, omplexolysis nd prodution of orgni id to soluilize fixed K in minerls musovite, ortholse, iotite, feldspr, illite, nd mi

9 Smpling Sites

10 Physio-hemil properties of Soils Prmeters Soil 1 To Field (MIYANOIRI) Soil 2 Soyen Field (TAKANISHI) Soil 3 Apple Orhrd (TBK) Prent Mteril Alluvil Alluvil Volni Texturl lss Sndy ly lom Cly Sndy lom Snd (%) nd Silt (%) 6 21 nd Cly (%) nd ph Orgni Mtter (%) CEC (mol (+) kg -1 ) Exhngele K (mol kg -1 ) Exhngele Cs(Bq kg -1 ) 79 * 107 Cs tivity (Bq kg -1 ) * -elow detetion limit nd not determined To onvert K mol (+)/kg x 390 = mg/kg

11 Methodology Isoltion of KSB

12 Chrteriztion of KSB Moleulr hrteriztion 16s RNA gene sequening Quntittive K soluiliztion

13 Chrteristi of 5 seleted KSB isoltes Bteril Isoltes D/d (rtio) K Soluiliztion (μgml -1 ) Isoltes Gener Billus megterium Pseudomons putid frederiksergensis Burkholderi sie Pseudomons mndelii

14 Pot Experiment Preprtion of soil, smple gmm steriliztion Initil soil nlysis Potting KSB Inoultion Inution nd equilirtion

15 Pot Experiment Germintion test Plnting Cre nd mintenne Hrvesting Wshing Weighing Grinding Rdiometri nlysis Plnt utordiogrphy* Sttistil nlysis

16 Clultions Dey orretion Trnsfer ftor in oth shoots nd roots Trnslotion rtio (TR)

17 Results Effets of KSB isoltes on shoot nd root yield Soil Isoltes Shoot (g) Root (g) Totl Biomss (g) Inrese (%) Control 0.67 ± ± ± ± Soil ± ± ± ± ± ± ± ± Soil 2 Control 0.57 ± ± ± ± ± ± ± ± ± ± ± ± Soil 3 Control 0.93 ± ± ± ± ± ± ± ± ± ± ± ±

18 Control Control Control Plnt iomss (g pot-1) Results Soil 1 Soil 2 Soil 3 Effets of KSB isoltes on totl iomss

19 Results Intertion effets etween soil nd teril inoulnts on shoot nd root dry weight

20 Results Plnt visul symptoms of possile rdiotoxiity Cs interferene in K metolism in plnts

21 Rdioesium umultion in shoot nd root

22 Results Intertion effets etween soil nd teril inoulnts on root nd shoot Cs

23 Control Control Control Trnsfer ftor d d d e 0 Soil 1 Soil 2 Soil 3 Trnsfer ftors in shoot

24 Control Control Control Trnsfer ftor Soil 1 Soil 2 Soil 3 Trnsfer ftors in root

25 Control Control Control Trnslotion rtio Soil 1 Soil 2 Soil 3 Trnslotion rtio

26 Results Intertion effets etween soil nd teril inoulnts on Cs trnslotion rtio (TR)

27 Control Control Control Shoots K (mg pot-1) Soil 1 Soil 2 Soil 3 K plnt uptke (shoots)

28 Control Control Control Root K (mg pot-1) d 0 Soil 1 Soil 2 Soil 3 K plnt uptke (roots)

29 Plnt iomss shoot+root (g pot-1) Plnt K uptke (mg pot-1) Reltionship etween plnt potssium uptke nd totl iomss

30 Shoot trnsfer ftor (TFs) Soil exhngele K (mol kg-1) Reltionship etween soil exhngele K nd shoot trnsfer ftor

31 Root trnsfer ftor (TFs) K (mol/kg) Reltionship etween soil exhngele K nd root trnsfer ftor

32 Conlusions KSB inoultion offers enefiil effets on plnt growth nd inresed the overll plnt iomss prodution KSB inoultion signifintly inresed the rdioesium umultion, with muh greter mgnitude in roots thn in shoots KSB inoultion my e essentil in mnging nd mnipulting rdioesium trnsfer from soils to plnts workers interested in mnging soil-to-plnt trnsfer of rdioesium might onsider the enefits of KSB on plnt growth promotion nd rdioesium umultion

33 Reommendtions supplementry reserh is needed on plnts grown under tul field onditions in order to mke resonle preditions on the influene KSB inoultion on plnt growth nd rdioesium umultion espeilly if the tehnology will e used to inrese the effiieny of miroe-ssisted phytoextrtion.

34 Aknowledgements Ministry of Edution, Culture, Sports, Siene nd Tehnology (MEXT) Nuler Sfety Regultion Authority (NSRA) of Jpn Tokyo University of Agriulture nd Tehnology (TUAT)