Role of Metalized Reflective Mulch with Nutritional and Insecticidal Treatments in Mitigation of HLB in New Citrus Plantings

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1 Role of Metalized Reflective Mulch with Nutritional and Insecticidal Treatments in Mitigation of HLB in New Citrus Plantings Mini-Field Day, A Duda & Sons Old Nursery 19 June 2015 Phil Stansly, Scott Croxton & Joby Sherrod w/funding from CRDF Table 1. Summary of cultural practices used for polyethylene mulch, insecticide, and foliar nutrition evaluation in citrus production with drip irrigation. Field Summary Location Duda Grove LaBelle, FL Experimental Design Split plot Randomized Complete Block Design: Main plots: (1) foliar nutrition alone, (2) insecticide alone, (3) both, (4) neither Subplots: with and without metalized mulch. Irrigation 2, 2gal/hr BowSmith Drippers per tree + flood for freeze protection Tree Spacing 9 ft in row 23 between rows = 208 trees/acre Area 10 acres Planting Date July 3-4, 2012 Scion rootstock Hamilin orange on Carrizo citromelo Rows North-South

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3 Table 2. Insecticide applications from planting to present. Insecticide Program Date Product Active Ingredient Rate Method Areas Treated July 9, 2012 Nuprid Imidacloprid fl oz/tree Drench Insecticide Plots October 11, 2012 Verimark Cyantraniliprole fl oz/tree Drench Insecticide Plots October 112, 2012 Intrepid Methoxyfenozide 12 fl oz/ac Drench Entire Field January 30, 2013 Platinum Thiamethoxam oz/tree Drench Insecticide Plots March 7, 2013 Nuprid Imidacloprid fl oz/tree Drench Insecticide Plots March 7, 2013 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field May 7, 2013 Verimark Cyantraniliprole fl oz/tree Drench Insecticide Plots July 4, 2013 Agri-mek Abamectin 10 fl oz/ac Spray Entire Field August 1, 2013 Nuprid Imidacloprid 0.05 fl oz/tree Drench Insecticide Plots September 15, 2013 Closer Sulfloxaflor 5.25 fl oz/ac Spray Insecticide Plots September 14, 2013 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field October 8, 2013 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots January 14, 2014 Danitol Fenpropathrim 16 fl oz/ac Spray Entire Field February 3, 2014 Platinum Thiamethoxam oz/tree Drench Insecticide Plots March 5, 2014 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots March 6, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Drench Entire Field May 1, 2014 Nuprid Imidacloprid 0.1 fl oz/tree Drench Insecticide Plots June 3, 2014 Agri-mek Abamectin 10 fl oz/ac Spray Entire Field June 25, 2014 Verimark Cyantraniliprole fl oz/tree Drench Insecticide Plots June 24, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field July 30, 2014 Belay Clothianidin 0.04 fl oz/tree Drench Insecticide Plots August 7, 2014 Agri-mek Abamectin 20 fl oz/ac Spray Entire Field September 3, 2014 Movento Spirotetramat 16 fl oz/ac Spray Insecticide Plots September 9, 2014 Intrepid Methoxyfenozide 12 fl oz/ac Spray Entire Field October 15, 2014 Delegate Spinetoram 6 oz/ac Spray Insecticide Plots January 8, 2015 Baythroid Beta-cyfluthrin 3.2 fl oz/ac Spray Entire Field January 27, 2015 Portal Fenpyroximate 32 fl oz/ac Spray Insecticide Plots March 24, 2015 AdmirePro Imidacloprid 14 fl oz/ac Drench Insecticide Plots May 1, 2015 Apta Tolfenpyrad 27 fl oz/ac Spray Insecticide Plots

4 Table 3. Foliar nutrition applications from planting to present. Foliar Nutrition Program Date Product Rate/Acre July 12, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal August 7, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal September 10, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal October 2, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal November 1, 2012 Fortress + N-Sure 1/2 lb + 1/2 gal February 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal March 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal April 2, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal May 7, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal June 3, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal July 3, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal August 1, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal September 10, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal October 8, 2013 Fortress + N-Sure 1/2 lb + 1/2 gal February 4, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal March 5, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal April 14, 2014 Soar Citrus Mix + N-Sure 1/2 gal + 1/2 gal May 1, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal June 4, 2014 Soar Citrus Mix + N-Sure 1/2 gal + 1/2 gal July 9, 2014 Griffin Green 1 gal August 5, 2014 Fortress + N-Sure 1/2 lb + 1/2 gal September 3, 2014 Griffin Green 1 gal October 15, 2014 Tricite lb November 10, 2014 Tricite lb January 20, 2015 Tricite lb February 26, 2015 Tricite lb March 10, 2015 Fortress + N-Sure 1/2 lb + 1/2 gal April 7, 2015 Griffin Green 1 gal May 26, gal

5 Table 4. Mean (±SEM) canopy and soil temperatures recorded over a 24 month period every 20 min at center canopy and at 5.08 cm soil depth. Low 0.1% a High 0.1% b All Observations Hours over 38 0 C Mulch Canopy 2.16± ± ± No Mulch Canopy 5.68± ± ± Mulch Soil 9.78± ± ± No Mulch Soil 8.19± ± ± a Mean temperature for the lowest 0.1% of the recorded samples. b Mean temperature for the highest 0.1% of the recorded samples. Table 5. Yield and juice quality analysis of Fruit harvested 9 December Total Yeild (lbs) 20 Fruit Weight (lbs) 20 Fruit Juice (lbs) Control Control+PRM Foliar Nutrition Foliar Nutrition+PRM Insecticide Insecticide+PRM Insecticide+Foliar Nutrition Insecticide+Foliar Nutrition+PRM Acid Brix With Insecticide Without Insecticide With Foliar Nutrition Without Foliar Nutrition With PRM Without PRM

6 Figure 1. ACP sticky card captures recorded every two weeks from July 2012 to present.

7 Figure 2. ACP infested flush recorded every two weeks from July 2012 to present.

8 Figure 3. Trunk area measurements taken every 6 months from July 2012 to present.

9 Figure 4. HLB leaf samples taken every 6 months from July 2012 to present.