Genotype and Growth Stage Effects on Heat Stress Susceptibility in Wheat

Transcription

1 Genotype and Growth Stage Effects on Heat Stress Susceptibility in Wheat Henry M Barber Monogram 2016 h.m.barber@pgr.reading.ac.uk

2 Heat Stress Grain set failure in field grown UK wheat (but modified environment) associated with days above 30 o C, during the five days before anthesis Ferris R et al Effect of high temperature stress at anthesis on grain yield and biomass of field-grown crops of wheat. Annals of Botany 82,

3 Grain yield relative to maximum 6 June Effect of 3- day pot transfers Savannah Alghabari et al (2014) Effect of Rht alleles on the tolerance of wheat grain set to high temperature and drought stress during booting and anthesis. Journal of Agronomy and Crop Science 200, Temperature at flowering ( o C)

4 Susceptible Timings Experiment in Rice: SATAKE, T. & YOSHIDA, H High Temperature-Induced Sterility in Indica Rices at Flowering. Japan Journal of Crop Science, 47, CRAUFURD, P. Q., VADEZ, V., JAGADISH, S. V. K., PRASAD, P. V. V. & ZAMAN-ALLAH, M Crop Science experiments designed to inform crop modeling. Agricultural and Forest Meteorology, 170, 8-18.

5 DH Population Savannah (Rht-D1b + 1BL/1RS) x Renesansa (Ppd-D1a x Rht8) 62 lines genotyped at JIC

6 Savannah (Rht-D1b + 1BL/1RS) x Renesansa (Ppd-D1a x Rht8) BARBER, H. M., GOODING, M. J. & SEMENOV, M. A Improving modelling of wheat responses to high temperature stress under climate change. European Society of Agronomy 13th Congress. Debrecen, Hungary: European Society of Agronomy.

7 Project Overview Year 1: Heat stress trial on DH parents focussing on timing of stress Smaller Experiment on Paragon NILs Year 2: Heat Stress Trial on all DH population Most susceptible GS s Assess for tolerance to HS

8 Identifying the susceptible timings Spikelet weight data, Grain yield data to be published. Stress Treatment Control Treatment Published in: BARBER, H. M., CARNEY, J., ALGHABARI, F. & GOODING, M. J Decimal growth stages for precision wheat production in changing environments? Annals of Applied Biology, 166,

9 Comparing the two Parents Significant effects on grain number Some grain size compensation at booting, especially from Savannah Parents most susceptible at different stages Clear differences in response at anthesis Double Gaussian data relative to GS65 to be published.

10 Was it Rht8 Effects? 5 timings in experiment Significant effects on grain number at booting and early flowering Temperature x timing effects No significant effect of Rht8 on response Paragon NILs comparison comparing rht8, described in: KOWALSKI, A. M., et al Agronomic assessment of the wheat semi-dwarfing gene Rht8 in contrasting nitrogen treatments and water regimes. Field Crops Research.

11 Comparing the whole population 3 timings in experiment: Early booting, Mid Booting, onset of anthesis Significant variation in fertility response to high temperature between genotypes Interactions with GS within each timing Allele effects at anthesis of Ppd-D1 and Rht-D1 QTL analysis to be conducted Results published in near future

12 Key Points Double dip response seen Very discrete differences in HS response due to GS This response influenced by genotype Both timings need stress when comparing different genotypes Grain Size compensation variable due to genotype and environment. Grain Number rather than yield needs to be studied No evidence Rht8 increases tolerance to heat stress Ppd-D1 and Rht-D1 influence fertility response at anthesis

13 Future Work Why are these effects around anthesis but not at meiosis? Why does Ppd-D1 and Rht-D1 cause differences in response? Large variation in response in DH population Find out why unexplained variation occurred- QTL? Crop Modelling?

14 Thanks for Listening! Acknowledgements: Mike Gooding, Martin Lukac, Mikhail Semenov BBSRC and the University of Reading Caroline Hadley, Liam Doherty, Richard Casebow, Laurence Hansen Richard Kino, Christina Clarke, Sam Leigh, Jacob Bishop, Pete Mark, Peter Jackson, Mike Garratt