NORDIC PUBLIC- PRIVATE- PARTNERSHIP IN PRE- BREEDING FOR DISEASES RESISTANCE IN BARLEY. Ahmed Jahoor

Transcription

1 NORDIC PUBLIC- PRIVATE- PARTNERSHIP IN PRE- BREEDING FOR DISEASES RESISTANCE IN BARLEY Ahmed Jahoor

2 Remarks on different interest between breeders and scientists Basic Research vs. Applied research Competition vs. Cooperation Individual vs. Integrated projects Publication vs. Application Model plant vs. Crop plants Simple traits vs. Complex traits Gene sequence vs. Gene action Short term vs. Long term projects

3 WHERE?

4 PPP = public private partnership on pre-breeding in plants Nordic collaboration in pre-breeding Public Private Partnership Involving Nordic plant breeders Nordic agricultural universities NordGen Co-funded by the 5 Nordic ministries of Agriculture

5 Nordic PPP- consortium for barley Jens Due Jensen, Jihad Orabi, Ahmed Jahoor: Nordic Seed, Denmark Therese Christerson, Stine Tuvesson, Bo Gertsson: Lantmännen Lantbruk, Sweden (only in the 1. phase) Gunter Backes: University of Copenhagen and University of Kassel (only 1. phase) Lars Reitan, Muath Alsheikh, Stein Bergersen: Graminor, Norway Reino Aikasalo, Mika Isolahti, Hanna Haikka, Outi Manninen, Merja Veteläinen: Boreal Plant Breeding, Finland Marja Jalli: MTT Agrifood Research Finland, Finland (from 2. phase) Lene Krusell, Rasmus L. Hjortshøj, Birger Eriksen: Sejet Plant Breeding, Denmark Therese Begtsson, Inger Åhman: Swedish University of Agricultural Sciences Magnus Göransson, Áslaug Helgadóttir: Agricultural University of Iceland, Iceland Coordinator: Ahmed Jahoor

6 EXAMPLES OF POOR TRANSFERABILITY OF LINKED MARKER IN WHEAT Disease Marker Reference Eyespot Pch1_orw1 Leonard et al Eyespot Pch1_orw6 Leonard et al Eyespot Xcfd175 Meyer et al Orange wheat blossom midge WM1 Thomas et al Hieght Rht-B1a Ellis el al. 2002,

7 The project work packages and material 1. Database development and association mapping 2. Disease resistance 3. Climatic changes 4. Preparing for the future Each breeders provided 30 breeding lines

8 Genotyping with single neclutide polymorphism markers (snps) DNA was extracted from all 180 lines (from single seedling) and 2000ng sent to TraitGenetics for genotyping with 9K-SNP iselect Illumina platform (7864 SNP markers) A total of 6996 SNP markeres were passed (89%) 6208 polymorphic SNP markers (89%) Only one line (PPP_0033) was failed among 180 PPP lines

9 Pairwise modified-roger's distances between breeders mrogers BOREAL Graminor LBHI LSW Nordic Seeds Sejet BOREAL 0.00 Graminor LBHI LSW Nordic Seeds Sejet

10 PPP project material at korpa, iceland September 2012 field visit Jónatan, Áslaug, Lars, Jahoor Juni 2012 PPP meeting March 2013 September 2012

11 Summary of disease phenotyping activities Disease/toxin Powdery Mildew Bipolaris Fusarium 1 1 Net Blotch Net Form Net Blotch Spot Form Nematode 1 1 Ramularia Scald Leaf Rust 2 DON 3 NIV Disease/toxin Loca9on Year Powdery Mildew Nordic Seed 2014 Powdery Mildew Lantmännen Svalöv 2014 Powdery Mildew Graminor 2014 Bipolaris Boreal 2014 Fusarium Graminor 2014 Net Blotch Net Form Boreal 2014 Net Blotch Net Form Nordic Seed 2014 Net Blotch Net Form Graminor 2014 Net Blotch Spot Form Boreal 2014 DON Graminor A- DON Graminor A- DON Graminor 2012 NIV Graminor 2012 Ramularia Lantmännen LaberweinPng 2014 Scald Boreal (Jokoinen) 2014 Scald Nordic Seed 2014 Leaf Rust Nordic Seed 2014 Leaf Rust Lantmännen Svalöv 2014 Plus cleistogamy screening with 2 KASP markers, SLU, 2014

12 Genome wide association scan for powdery mildew infection in two locations Correlation between powdery mildew two disease assessments Pm.Lm.12 Pm.Ns.12 Pm.Lm Pm.Ns upper right: pearson r, lower left: error probability Several SNPs with significantly high association to ppowdery mildew resistance were found on chromosome 4H

13 Validation of nematode resistance markers PPP name Nem- r1 Nem- r2 Marker 1 Marker 2 PPP_ A A PPP_ A A PPP_ A A PPP_ A A PPP_ C A PPP_ C A PPP_ C A PPP_ C A PPP_ A G PPP_ A G PPP_ A G PPP_ A G PPP_ C G PPP_ C G PPP_ C G PPP_ C G

14 Genome wide association scans for scald disease

15 Acri and Rrs2 markers Rrs2 T C Acri Digested 21 2 Not digested On the barley physical map Rrs2 marker is around 7Kb and Acri on around 16 Kb

16 Fusarium markers on 5H The best markers on 5H is on the long arm in an interval of 5 cm ( cm) One QTL with quantitative resistance (qrfg-5h.13) is located close to this region (Dahleen et al. 2003)

17 Summary of agronomic traits phenotyping activities # Development Height Straw Yield 1 Heading date_51_iceland_2012 Height 1_Iceland_2012 Lodging_Iceland_2012 TGW (g) LBHI 2012, BLUP 2 Heading date_51_iceland_2013 Height 1_Iceland_2013 Lodging_Iceland_2013 TGW (g) LBHI 2013, BLUP 3 Heading date_sejet_2012 Height 1_Sejet_2012 Lodging_Iceland_2014 Dry ma_er grain (%) at harvest LBHI 2012, BLUP 4 Heading date_sejet_2013 Height 1_Sejet_2013 Lodging at ripening_graminor_2014 Dry ma_er grain (%) at harvest LBHI 2013, BLUP 5 Heading date_lantmännen Bjertop_2012 Height 2_Iceland_2012 Lodging_Sejet_2014 Grain dry yield (t/ha) LBHI 2012, BLUP 6 Heading date_lantmännen Bjertop_2013 Height 2_Iceland_2013 Awn breaking_iceland_2012 Volume weight (g/l) LBHI 2012, BLUP 7 Heading date_lantmännen Svalöf _2012 Height 2_Sejet_2012 Awn breaking_iceland_2013 Volume weight (g/l) LBHI 2013, BLUP 8 Heading date_lantmännen Svalöf _2013 Height 2_Sejet_2013 Ear Breaking_Sejet_2012 Dry weight (g) Graminor Heading date_lantmännen Svalöf _2014 Height 3_Iceland_2012 Ear Breaking_Sejet_2013 Yield kg/da 15% Graminor Heading date_lantmännen LaberweinPng _2012 Height 3_Iceland_2013 Necking_Iceland_ Heading date_lantmännen LaberweinPng _2013 Height 3_Sejet_2012 Necking_Iceland_ Heading date_lantmännen LaberweinPng _2014 Height 3_Sejet_2013 Straw Breaking_Iceland_ Heading date_nordic Seed_2012 Height 4_Iceland_2013 Straw Breaking_Iceland_ Heading date_nordic Seed_2013 Straw Length_Iceland_2012 Straw Breaking_Iceland_ Heading date_boreal_2013 Straw Length_Sejet_2012 Straw Breaking 1_Sejet_ Heading date_iceland_2012 Straw Length_Iceland_2013 Straw Breaking 1_Sejet_ Heading date_51_iceland_2013 Straw Length_Sejet_2013 Straw Breaking 2_Sejet_ Maturity_59_Iceland_2012 Straw Length_Lantmännen Bjertop_2012 Straw Breaking 2_Sejet_ Maturity_87_Iceland_2012 Straw Length_Lantmännen Svalöf _2012 Straw Breaking 3_Sejet_ Maturity_87_Sejet_2012 Straw Length_Lantmännen LaberweinPng _2014 Straw Breaking 3_Sejet_ Maturity_87_Sejet_2013 Straw Length_Nordic Seed_2013 Straw Breaking 4_Sejet_ Maturity_87_Boreal_2013 Straw Length_Graminor_2013 Straw Breaking 4_Sejet_ Heat sum_87_iceland_2012 Straw Length_Graminor_2014 Sha_ering_Iceland_ Heading date_graminor_ Days from sowing to ripening_ Graminor_ Days from sowing to heading_graminor_ Days from sowing to heading_graminor_ Days of Ripening from sowing_ Graminor_ Ripening date of Sep_Graminor_ Days of Ripening from new year_graminor_ dg aks- gm_graminor_2013 Straw height before harvest (ground to below axe), Iceland_2014 Sha_ering_Iceland_2013 Grain yield (g/plot) Graminor 2014

18 Development of MAGIC populations PPP activities 4

19 Summary Nordic PPP established and runs projects Corporation among breeding companies Corporation between public private partners Development and management of database in Validation of DNA markers Association mapping for disease resistance Association mapping for climate changes Preparing for future