Diamond V Beef Research & MOA

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1 Diamond V Beef Research & MOA

2 Optimize the Rumen Metabolites. Metabolites Rumen Bacteria Yeast Culture Fermentation Appetite Dry Matter Intake Fermentation Net Energy Microbial Protein Palatability Factors Maintenance Growth Immune Response

3 Fiber Digesting Bacteria OD, 600nm * 1.8* 1.9* % Original XP Filtrate 5% Original XP Filtrate 0.3* 0.5* F. succinogenes R. flavefaciens R. albus 0.8* *Different from at P<.05 Callaway and Martin J. Dairy Sci. 80:

4 Lactate-utilizing Bacteria % Original XP Filtrate 5% Original XP Filtrate 0.43* OD, 600nm * 0.17* 0.14* 0.10* 0.29* 0.10* 0.30* 0.0 Selenomonas ruminantium Megasphaera elsdenii *Different from at P < 0.05 Callaway and Martin J. Dairy Sci. 80: * HD4 H18 B159 T81

5 Lactate Utilizers 14 ruminally cannulated steers - Grazing wheat pasture Treatments: 0, 28, or 56 g XP Rumen fluid Bacterial populations (Pyrosequencing) Results: Increase abundant genera Ruminococcus, Succinoclasticum, Sporobacterium and Butyrivibrio Bacterial genera identified only in the SFP supplemented steers Minor species - functionally vital to enhance ruminal fermentation Lactate utilizers - Lactobacillus, Lactococcus, Megasphaera, Atopobium and Enterococcus Sulfate reducers - Fusibacter, Therminicola, Synechococcus, Desulfosporosinus Pitta et al J. Anim. Sci. 88 (E-Suppl. 2): 702.(BF404-a)

6 Lactate Utilizers Figure 5a/b: Venn diagrammatic representation of minor genera present in fiber and liquid fractions of 3 treatments across all days of sampling (underlined genera are lactate utilizing bacteria) Pitta et al J. Anim. Sci. 88 (E-Suppl. 2): 702.(BF404-a)

7 Keep the Rumen Healthy! Lactate, mm P < 0.03 Original XPC P < d after 2 d after 5 d after 12 ruminally fistulated heifers (277 kg) fed 80% fescue hay and 20 % alfalfa pellets for 3 weeks then a 90% concentrate diet for 2 weeks. or Original XPC. Moya et al J. Anim. Sci. 87: (BF016-j)

8 Keep the Rumen Healthy Height, cm Original XPC Strength, min Original XPC P < Foam Height 0 Foam Strength 12 ruminally fistulated heifers (277 kg) fed 80% fescue hay and 20% alfalfa pellets for 3 weeks then a 90% concentrate diet for 2 weeks. Moya et al J. Anim. Sci. 87: (BF016-j)

9 E. coli 8 Charolais heifers, ruminally cannulated, negative for O157 Treatments: 0 or 56 g XP (30 days pre-challenge to 33 d post challenge) Challenged with single dose (10 10 CFU) O157 directly in rumen Rectal-Anal juncture (RAJ) mucosal swabs (RAMS), rumen digesta Results: XP heifers carried fewer O157 for shorter duration in both rumen at RAJ mucosa Reduced survival of E. coli O157:H7 in the rumen and reduced the duration of bacterial colonization of the bovine RAJ mucosa Liou et al Prof. Anim. Sci. 25: (403-j)

10 E. coli Ruminal burdens 24 h post-challenge RAMS burdens 24 h post-challenge O157 Concentration, CFU/ml Original XP P = O157 Concentration, CFU/ml Original XP P = Frequency of E. coli O157:H7 enumerated in cultures of RAMS 120 O157 Positive (%) Original XP Days Post -Challenge Liou et al Prof. Anim. Sci. 25: (403-j)

11 Beef Product Portfolio Original Brands Non-GMO Formulas OMRI Formulas Organic Selenium DFM Brand Original XPC XPC LS Non-GMO XPC Green SelenoSource AF 2000 XP -DFM Original XP XP LS Non-GMO XP Green SelenoSource AF 2000 Original YC SelenoSource AF 600

12 Feeding Guidelines 1 Stage Original YC Original XP Original XPC XP DFM Creep Fed Calves 62.5 lb/ton (31.2 kg/mt) 25 lb/ton (12.5 kg/mt) 6.25 lb/ton (3.1 kg/mt) 25 lb/ton (12.5 kg/mt) Starting/Receiving Cattle 5.0 oz (140 gm)/hd 2.0 oz (56 gm)/hd 0.50 oz (14 gm)/hd 2.0 oz (56 gm)/hd Stocker Cattle 2.5 oz (70 gm)/hd 1.0 oz (28 gm)/hd 0.25 oz (7 gm)/hd 1.0 oz (28 gm)/hd Grower/Finisher Cattle 2.5 oz (70 gm)/hd 1.0 oz (28 gm)/hd 0.25 oz (7 gm)/hd 1.0 oz (28 gm)/hd Breeding Animals 2.5 oz (70 gm)/hd 1.0 oz (28 gm)/hd 0.25 oz (7 gm)/hd 1.0 oz (28 gm)/hd Show Feeds 5.0 oz (140 gm)/hd 2.0 oz (56 gm)/hd 0.50 oz (14 gm)/hd 2.0 oz (56 gm)/hd 1 Values above represent rates per day Plus applicable Green products

13 Diamond V The Rumen Optimizer Starting Cattle Info

14 Intake of Shipping-Stressed Calves Original YC Dry Matter Intake, lb/day Week 1 Week 2 Week 3 Week 4 Week 1 Week 2 Week 3 Week 4 Steers Heifers 23 steers and 18 heifers (400 lb) were subjected to shipping stress, allocated to 3 pens and fed 28 d. Treatments shown include and 2% Original YC. Phillips and VonTungeln USDA, El Reno, Oklahoma. Nutr. Rep. Int. 32:

15 Steers Challenged With IBRV 24 Dry Matter Intake, lb/d ** Original XP * * ** ** * P < 0.10 ** P < 0.05 ** * Days Post-Inoculated wtih IBR Virus 30 crossbred steers acclimated to diet for 14 d, then challenged with IBRV (intranasally). or Original XP. Cole et al J. Anim. Sci. 70: (BF104-j)

16 Receiving Cattle: Stress Percent Treated as Sick Total Number Sick Days 50 P < P < 0.05 % Sick Number Days Forage then 72% Concentrate 72% Concentrate 0 Forage then 72% Concentrate 72% Concentrate Original XP Original XP 112 male calves (crossbred), 166 kg BW, 56 d, Transport stress 30 hours Treatments: 2 x 2 72% concentrate receiving diet or Forage based diet for 7 days, then 72% concentrate 0 or 28 g XP (for 28 days) Diets: Concentrate: 54% flaked corn, 14% alfalfa hay, 14% sudangrass hay, 8% can molasses, 2% fishmeal, 4% CSM, and 2% yellow grease fat Forage diet: 46.5% alfalfa hay, 46.5% sudangrass hay, and 7 % cane molasses Zinn et al J. Anim. Sci. 77 (Suppl. 1): 113.

17 ADG of Low-Risk Calves 5.0 Original XPC 28-Day ADG (lb/hd/day) Overall ADG Response: lb/hd/day (P < 0.01) 0.0 Group 1 Group 2 Group 3 Group 4 Group 5 74 low-risk calves were weaned and allocated to 10 pens. Diet consisted of coarse-textured, corn-based starter containing AS-700. or Original XPC. Belknap et al J. Anim. Sci. 85 (Suppl. 1):551.

18 Intake of Low-Risk Calves 25 DM Intake (lb/hd/d) Original XPC Day 74 low-risk calves were weaned and allocated to 10 pens. Diet consisted of coarse-textured, corn-based starter containing AS-700. or Original XPC. Belknap et al J. Anim. Sci. 85 (Suppl. 1):551.

19 Performance of Low-Risk Calves XPC P < Initial BW, lb NS Final BW, lb DMI, lb/d ADG, lb/d F/G, lb/lb NS 74 low-risk calves were weaned and allocated to 10 pens. Diet consisted of coarse-textured, corn-based starter containing AS-700. or Original XPC. Belknap et al J. Anim. Sci. 85 (Suppl. 1):551.

20 Diamond V The Rumen Optimizer Finishing Cattle Info

21 Iowa Field Trial 380 steers (348 kg) Allocated to two pens in hoop barn 161 d Treatments: Diet: 14 g XPC in a pellet mixed into a TMR 24% whole shelled corn, 26% high moisture corn, 21% earlage, 8% wet corn gluten feed, 5% modified wet distillers grains, 14% hay, 2% balancer

22 Iowa Field Trial Original XPC DMI, kg/hd/d A Reimplant (day 57) B C D Days on Feed Cause of stress A = Delayed reaction to processing or Diet B = Replace bedding C = Replace bedding D = Heat stress Overall DMI = kg XPC = kg Iowa Field study

23 Iowa Field Trial ADG, kg Feed: Gain, kg/kg HCW, kg Dressing Percentage, % Iowa Field study Original XPC 62.0

24 Nebraska Field Study 2214 steers (260 kg), 14 pens 117 d Treatments: 56 g XP for 28 d, then 28 g thereafter Diet: 63% DRC, 12% corn silage, 11% wet DGS, 6% sunflower meal, 5% liquid supplement, and 3% alfalfa hay on DM basis Monensin and Tylosin Western Feedlot study

25 Nebraska Field Study 1.6 Original XP 7.1 Original XP ADG, kg Intake, kg Feed:Gain, kg Original XP Starch Digestibility, % Original XP Western Feedlot study

26 Feedlot Studies Summary of feedlot studies 25 feedlot trials 5278 heifers, steers, and bulls Average days on feed = 92 d Locations: Diet: US (21), Canada (2), Mexico (1), UK (1) Majority TMR Treatments: Yeast Culture (YC, XP, or XPC) Iowa Field study

27 Daily Gain (kg) Feed Efficiency Trial No. Diamond V Yeast Culture Difference BF BF BF BF104a BF104b BF BF BF BF004a BF004b BF005a BF005b BF BF008a BF008b BF BF BF BF BF013a BF013b BF BF BF BF Average Trial No. Diamond V Yeast Culture Difference BF BF BF BF104a BF104b BF BF BF BF004a BF004b BF005a BF005b BF BF008a BF008b BF BF BF BF BF013a 4, BF013b BF BF BF BF

28 Performance Average Daily Gain Feed Efficiency kg Feed:Gain Yeast Culture Yeast Culture Summary of 25 Trials Representing a total of 5278 animals

29 Diamond V The Rumen Optimizer Grazing Cattle Info

30 Ohio Pasture Study 2 Experiments (Exp.) Exp. 1: 20 Angus x Hereford cows Exp. 2: 40 cows and their calves Treatments: Mineral Mineral containing 25% XP Diet: Grazing pasture grass (80% Kentucky Fescue) Kobs and Boyles Masters Thesis.

31 Ohio Pasture Study 200 ab P < 0.01 Mineral Mineral + Original XP Intake, g/hd/day a b Total Mineral Mix Mineral Only Average from December through August Kobs and Boyles Ohio State Univ.

32 Ohio Pasture Study 60 Organic Matter Digestibility, % P = P = December June August Overall Original XP 70 NDF Digestibility, % P = P = December June August Overall Kobs and Boyles Masters Thesis. Original XP

33 Ohio Pasture Study Milk Production (Day 120), kg P = P = P = Year 1 Year 2 Overall Original XP 195 Weaning Weight, kg Year 1 Year 2 Overall Kobs and Boyles Masters Thesis. Original XP

34 Calf Weaning Weight 200 Original XP kg pairs provided with 2:1 mineral mix with or without 20% Original XP. Eastern Producer Field Study

35 Daily Gain Of Grazing Calves kg/hd/day Original XP crossbred steers (347 kg) were grazed fescue pasture for 99 days. Provided 2:1 mineral mix with or without 25% Original XP. Eastern Producer Field Study

36 Questions?