Improving legume cultivation through breeding and management: LEGATO

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1 Improving legume cultivation through breeding and management: LEGATO LEGumes for the Agriculture of TOmorrow (LEGATO) 17 academic partners 10 industrial (SME or larger) or associations (6 breeding, 2 molecular biology, 2 food processing) INRA Transfert for assistance with project management IAMZ-CIHEAM training/dissemination Budget breakdown Total budget ca. 8M 5M EU subsidy, of which: 770K SME Projected start date: 1 january 2014

2 WP8 R.Thompson Project management WP2 D.Rubiales Biotic stress resistances WP5 E.S.Jensen Cropping system management WP3 C.Salon Optimizing responses to abiotic stresses WP1 P. Annichiarico Genetics and new breeding tools and material FP7 LEGATO Scientific coordination: G. Duc WP4 C.VazPatto Defining traits adapted to consumers expectations in foods WP6 R. Ward stakeholder interface: targets and evaluation WP7 D. Gabina Outreach

3 WP1 Genetics: New Breeding tools and material -regulating yield (eg., mfn genes) -marker development and testing - Development of allele-specific markers - Assessment of genetic gain from marker-assisted selection in pea -high density marker collections: - CSSL introgression lines - high density sequence based genetic map (RAD markers on JI281 x Caméor)

4 WP2: Novel sources of resistance to pests and diseases M. pinodes

5 WP3 Optimizing plant adaptation to abiotic stress Optimizing roots for N-uptake during drought Drought tolerance in pea and lupin (genetic and physiological basis) Increasing plant performance under combined drought and heat stress through favourable alleles

6 Nutritional quality Ex: Chromatographic profiles of phenolic compounds in two legume seed samples (green and red) detected by HPLC-DAD. Compounds detected by LC-MS (1-Catechin; 2-Caffeic Acid; 3-Quercetin-3-O-Rutinoside; 4- Kaempferol-3-O-glucoside) WP4 Defining traits adapted to consumers expectations Chromatographic profiles obtained with HPLC-DAD (violet) and HPLC-ED (red) for one legume sample (red dot indicate the compound, Catechin, with ED signal, revealing possible antioxidant activity) Organoleptic and end-user quality Consumers preferences Viscosity cp Graphical Analysis Results Newport Scientific Pty Ltd Time mins Temp 'C Ex: Rapid Visco Analyser curves (rheological properties) from legume flours of whole seeds before soaking (blue) and of cotyledons obtained from dehulled seeds after soaking (red) corresponding to different pasting behavior Ex: Consumer sensorial analysis of innovative legume based food products such as fortified cereal breads Ex: Spectrocopic ATR/FT-IR screening models Breeding tools for quality

7 WP5: Grain legumes cropping system management Institut za ratarstvo i povrtarstvo (IFVCNS)

8 WP5 Objectives 1. To prototype and assess the sustainability and potential adoption of novel cropping systems with grain legumes (GL) grown as sole crops (SC), as variety mixtures (VM) or intercrops (IC). 2. To determine the effect of novel diversified GL designs (VM and IC) on production, yield stability, symbiotic performance and impact on biotic stresses (diseases and weeds) in crop rotation sequences with GL and the underlying mechanisms 3. To develop methods for rapid determination of the requirement for inoculation with Rhizobium leguminosarum biovar viceae (Rlv) in pea and faba bean in European agro-pedoclimatic environments, including strain selection for inoculation.

9 Four WP5 tasks Task 5.1: Design and multi-criteria sustainability assessment of GL-based systems Task leader: INRA Grignon Task 5.3: Preventing biotic stress (disease, pest and weeds) by crop diversification Task leader: CSIC Task 5.2: Agronomic performance of diversified GL crops Task leader: SLU Task 5.4: Inoculation requirement of pea and faba bean and selection of strains Task leader: INRA Montpellier

10 WP6 Stakeholder interface for target orientation and practical evaluations Europe-wide trials of material identified in research WPs Economic feasibility evaluation Additional sites planned for field trials: RAGT, Jouffray Drillaud, Agri-Obtentions, Wherry, Limagrain, APSOV Sementi, Fertiprado, Jogeva PBI, (Estonia), Nafref Thermi (Greece)

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