Plant Pathology & IDM in Laos

Transcription

1 Plant Pathology & IDM in Laos Dr Kylie Ireland, Ms Sophia Callaghan & Professor Lester Burgess This presentation has been developed by Scope Global, a delivery partner for the AVID program.

2 Experience of a lifetime Video Agriculturally diverse Champasak The Big 6 plant diseases of Champasak Key projects Take home messages

3 Experience of a lifetime VIDEO

4 Champasak, an Agricultural Powerhouse Vegetables Head cabbage Chinese cabbage Tomato Eggplant Chinese vegetables (bok choy, choy sum, gai lan, mustard greens) China Myanmar Vietnam Laos Thailand Cambodia Champasak Province Lettuce Ginger Cucumber Squash Sweet peas Beans Coriander Maize Fruit Banana Durian Rambutan Pineapple Passionfruit Papaya Citrus Coffee

5 Big 6 Vegetable Diseases Rhizoctonia collar and root rot Bacterial wilt Root knot nematode Phytophthora blight of tomato Phytophthora root rot Sclerotinia head and stem rot

6 RHIZOCTONIA COLLAR AND ROOT ROT Fungus: Rhizoctonia solani 20-50% seedling loss Whole field stunting and yield loss

7 BACTERIAL WILT Bacteria: Ralstonia solanacearum

8 ROOT KNOT NEMATODE Nematode: Meloidogyne sp.

9 PHYTOPHTHORA BLIGHT OF TOMATO Oomycete: Phytophthora infestans

10 PHYTOPHTHORA ROOT ROT Oomycete: Phytophthora capsici

11 SCLEROTINIA HEAD AND STEM ROT Fungus: Sclerotinia sclerotiorum First discovery March 2014, by chance & good training! Found in field January 2015 Bad produce basket Laboratory 4 days later

12 Key Projects: Farmers First! Laboratory diagnostic training Ongoing, underpins all other activities Durian dieback management Cabbage survey Ah hoc & ongoing, check out Lester s poster! Vegetable IDM training Crawford Fund & APS Office of International Programs Scientific support Isolation, deposition & write up

13 1. Collect sample from the field ເກ ບຕ ວຢ າງພ ດຈາກພາກສະໜາມ

14 2. Isolation and identification in the lab. ນາມາແຍກແລະວ ໃຈຢ ໃນຫ ອງວ ໃຈ

15 3. Pathogenicity test ເຮ ດທ ດສອບຄວາມເປ ນພະຍາດຄ ນ 1. INOCULATE 2. OBSERVE & RECORD SYMPTOMS Day 1 Day 6 Day CONFIRM PATHOGENICITY 3. ISOLATE

16 4. Preservation of culture ເກ ບຮ ກສາຕ ວຢ າງ Herbarium sheets Culture collection Deposit in International culture collection

17 Durian Dieback Complex Education Smallholders District & provincial staff Greenhouse Helvetas DAP Grant Phosphite injection Dieback complex Phytophthora Mango stem borer

18 Cabbage Survey From 2009 Reduced yields Found Black rot Rhizoctonia Sclerotinia Absent Club root Phoma lingam Check out Lester s poster

19 Vegetable IDM Training: Train the Trainer

20 Vegetable IDM Training: Train the Trainer

21 Other projects Crawford Fund workshops Vegetable diseases Len Tesoriero Bacterial diseases Ric Cother Bakanae of rice survey Fusarium fujikuroi Alessandro Infantino & Virgilio Balmas Food safety survey Biomin Citrus health HLB & vector Entomology volunteers Savannakhet laboratory Vientiane policy support

22 The work continues Sophia Callaghan & Adam Fusarium wilt on watermelons Fusarium oxysporum niveum race(s)? Grafting onto bottle gourd Phytophthora Path testing P. capsici and P. drecsleri on chilli Sequencing - Treena Burgess and Diane White at Murdoch Soilless media for seedling production Affordable & accessible Coffee waste product, burnt rice hulls, sand, coconut fibre and cow manure

23 How can you help? Volunteer! Plant Biosecurity Policy, Vientiane Applications close 21st September! Support team list Deposit/sequence isolates And help with the write up! Donate Consumables, books, microscopes. Contact myself or Lester

24 Take home messages Relationships matter Language matters Open heart & open mind Basic technical skills + sustainable methods + commitment = success! Farmer s first!

25 Thanks - ຂອບໃຈ