Progress in Development and MAS of FHB Resistant Wheat Cultivars and Germplasm at Virginia Tech

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1 Progress in Development and MAS of FHB Resistant Wheat Cultivars and Germplasm at Virginia Tech Jianli Chen, Jody Fanelli, Carl Griffey, Joe Paling, M.A. Saghai Maroof, and Gina BrownGuedira Virginia Polytechnic Institute and State University, Blacksburg, VA 0

2 Objectives Incorporate, characterize, and combine scab resistance genes from newlydeveloped scab resistant and/or scab tolerant SRW wheat lines in development and deployment of commercially viable wheat cultivars via haplotype selection and breeding. Accelerate release of scab resistant cultivars and/or germplam using molecular marker assisted breeding in the characterization and selection for FHB resistance.

3 Summary of Progress High yielding, FHB resistant SRW wheat cultivars/germplasm have been developed using a combination of topcross, doubled haploid, backcross and marker assisted breeding methods (Table 8). Potential cultivar/germplasm release in 007 and 008 (Table ). Markerassisted assisted evaluation of three FHB QTL located on wheat chromosomes 3BS, AS, and 3AS conducted in parental lines, 0 threeway F progeny, 83 BCF progeny, and wheat lines in the 00 VT FHB Advance and Preliminary Tests. High throughput genotyping conducted at VT MAS lab and in collaboration with USDAARS ARS Genotyping Centers. Promising varieties and germplasm and valuable information on QTL and markers have resulted from support obtained from the U.S. Wheat and Barley Scab Initiative.

4 Table. Potential Cultivar/Germplasm Release at VT Wheat Breeding and Genetics Program Line Breeding Method Type I Incidence (Field %) Type III DON (Field) Type II Severity (GH) QTL Markers VA00W370 Topcross BSBarc33, Barc33, STS3B VA0W73 Topcross ASBarc0 VA0W38 Topcross ASwmc; 3BSBarc33, Barc33, STS3B; ASBarc7, Barc0 VA0W33 33 Topcross BSBarc33, Barc33, STS3B; ASBarc0 VA0W7 Doubled haploid 030 3ASwmc; 3BSBarc33, Barc33, STS3B VA0W7 7 Doubled haploid ASBarc7, Barc0 VA0W Backcross ASWmc; 3BS Gwm33 VA0W Backcross. 3BSBarc33 Barc33

5 Table. Performance of VA0W370 and VA0W73 in 00 Uniform Southern Soft Red Winter Wheat Nursery: 3 Sites Genotype Grain Yield Bu/A Rank Test Weight Lb/Bu Rank PM 0 LR % YR % SEPT 0 HF % 0 B VA0W GA3E GA3E AGS GA3E VA0W B USG VA0W MEAN

6 Table 3. FHB Resistance Breeding Stocks Developed and Evaluated by the Virginia Tech Wheat Breeding Program in 00. Breeding Stocks Populations Headrows Observation Preliminary Germplasm , ,

7 Table. Molecular Markers Used in the Current Study 3BS gwm33 barc33 STS3B STS3B gwm3 cfd07 Liu et al., 00; Chen et al., 00 AS barc7 barc8 barc00 gwm barc8 Buerstmayr et al., 00; Chen et al., 00 3AS gwm00 gwm7 barc0 wmc8 wmc Shen et al., 003; Somers, et al., 00; Chen et al., 00

8 Table. Haplotypes of the Three QTL in Known FHB Resistance Sources. Line QTL3AS QTL 3BS QTL AS Wmc Gwm33 Barc33 STS3B Gwm3 Barc7 Barc0 Sumai 3 W Futai8 Ning780 Ning0 Nobeoka Bozu Wngshuibai Frontana

9 Table. FHB Resistant Lines Developed by Different Breeding Methods, Two Year Performance Line Grain Yield (0) (0) Test Weight (0) (0) FHB Index (0) (0) (0) LR (0) Coker PionR Tribute 88 0 VA00W VA0W VA0W VA0W38 8 VA0W VA0W VA0W Grand Mean

10 VA0W370 Wmc QTL3AS QTLAS QTL3BS Line Barc0 Barc7 Gwm3 STS3B Barc33 Gwm33 VA0W VA0W3 VA0W33 VA0W38 VA0W30 VA0W73 VA00W38 Tribute PionR Coker 83 Table 7. MarkerAssisted Evaluation for Newly Developed FHB Resistant Line

11 Table 8. FHB Resistant Lines Developed by Different Breeding Methods, Two Year Performance. Grain Yield Test Weight FHB Index LR Line (0) (0) (0) (0) (0) (0) (0) (0) VA0W7 7 0 Renwood VA0W Pion VA0W VA0W Roane VA0W VA0W VA0W Grand Mean

12 Wmc QTL3AS Barc0 Barc7 Gwm3 STS3B Barc33 Gwm33 QTLAS QTL3BS Line VA0W VA0W7 VA0W3 Roane VA0W7 VA0W7 Pion8 VA0W Renwood30 VA0W7 Table. MarkerAssisted Evaluation for NewlyDeveloped FHB Resistant Line.

13 Table 0. FHB Resistant Lines Developed by Different Breeding Methods, 00 Performance, Warsaw Location VA0W Grand Mean VAW VA0W VA0W OH VA0W VA0W VA0W VA0W Tribute PionR Coker83 GH Severity Field DON ppm Field Severity % Field Incidence % Index PM 0 LR 0 Test Weight (Lb/Bu) Grain Yield (Bu/A) LINE

14 Table 0. FHB Resistant Lines Developed by Different Breeding Methods, 00 Performance, Warsaw Location VA0W73 GH Severity Field DON ppm Field Severity % Field Incidence % Index PM 0 LR % Test Weight (Lb/Bu) Grain Yield (Bu/A) LINE Grand Mean VA0W Pion VA0W VA0W Ernie VA0W VA0W VA0W VA0W Roane VA0W Renwood30

15 Acknowledgements Virginia Small Grain Board Virginia Agricultural Council USDAARS, ARS, U.S. Wheat & Barley Scab Initiative