Agricultural enterprise «Il GIRASOLE»

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1 Linea di Prodotti ECOENZIMI Trattamenti Enzimatici per l agricoltura, la Zootecnia e la Sanificazione Agricultural enterprise «Il Girasole» Agricultural enterprise «Il GIRASOLE» Enzyme treatment in agriculture

2 Linea di Prodotti ECOENZIMI In recent years, many corn farms of the Piemonte region have been affected by rootworm (Diabrotica virgifera), with significant crop losses, see the library is not permitted for the contamination level reached after the traditional treatment for pest control. 2 Corn rootworm Parasite recently emerged in Europe grub of the corn borer, the major pest of this crop in France Different action is proposed by., with the use of products based on ECOENZIMI, for: Enhanced root system Accelerate the germination Increase immunity Increase in reaction to pests Phasing out of synthetic products In this context, the enterprise "Il Girasole, which uses corn PIONEER an area of 25 hectares in the town of Trino (Vercelli, Italy), has mandated. to conduct the tests ECOENZIMI treatment on soil, stubble and leaf on the totality of his crop of corn.

3 Linea di Prodotti ECOENZIMI Agricultural enterprise: 3 Signor. Marello GUIDO 80, via Pontestura CAMINO - Alessandria - Italia P. IVA FC MRLGDU74H06B885K Tel: guido.marello@infinito.it The purpose of this report is to: Summarize the characteristics of products and their effects Introduce the principles of treatment Show the sequence of tests Analyze the results and show their comparative terms 1 - ENZYMATIC CHARACTERISTICS OF MIXTURES USED: CONCIAZYM, IAGROENZYM e STOPPIEZYM are natural products of biotechnology and biostimulating plant metabolism 1.1 CONCIAZYM Enzyme injection for the treatment of seeds for conventional and organic farming Enzyme mixture of natural ingredients designed for the treatment of cereal seeds during planting and regeneration of roots. The product increases the germination of seedlings with the rapid development of the root system, improving the resistance to cold and disease. The enzymes degrade the organic matter in soil, making it immediately available nutrients. The seeds so treated more easily resist the attacks of fungi, bacteria and insects. His compositions, characteristics and conditions of use are described in the datasheet. 1.2 IAGROENZYM Mixture of natural enzymes destined for the agricultural sector for the foliar treatment. The product performs the action of mineralization of the elements available in the soil by increasing the microbial population. Enzymes stimulate microbial activity, are biocatalysts and improve the nutrition of plants. The product increases the resistance to biotic agents (bacteria, insects, fungi, etc..) Agents and abiotic (drought, wind, frost, salinity, etc..). His compositions, characteristics and conditions of use are described in the data sheet.

4 Linea di Prodotti ECOENZIMI 1.3 STOPPIEZYM 4 Mixture of natural enzymes destined for the agricultural sector for the decomposition, after the threshing of wheat stubble, crop residues (corn, rice, wheat, barley, etc.).. The process facilitates the rapid transformation of stubble into stabilized humus, releasing nutrients to the microbial flora of the soil and plants. The rapid decay of stubble promotes the work of the soil and prevents insects that overwinter in crop residues. His compositions, characteristics and conditions of use are described in the data sheet. 2 - PRESENTATION OF TEST The company "Il Girasole", has provided about 25 hectares for maize production, using products ECOENZIMI. The trials were held together using agronomic techniques with low environmental impact: Treatment of stubble (STOPPIEZYM) or tillage to green manure. seed treatment with the mixture of enzyme (CONCIAZYM) Two foliar treatments with use of enzyme mixture (IAGROENZYM) with nitrogen fertilizers

5 Linea di Prodotti ECOENZIMI 1. The green manure is an ancient technique used by farmers to increase the biological fertility of soil and characterized the first phase of treatment (November 2008). The land was sown with vetch (villosa Vicia), white mustard (Sinapis alba), barley and treated with 2 kg of solution STOPPIEZYM diluted in water per hectare. 2. Seed treatment was carried out directly in the planter with a dose of g CONCIAZYM every 25,000 seeds This process has allowed a faster germination, development of a better root system deeper, resulting in very effective protection against pests fungi, insects and bacteria. The two foliar treatments began to appear in the 5 / 6 leaf of maize (May 2009) using IAGROENZYM of 2kg per hectare, with about 30 units of nitrogen foliar fertilizer per hectare as against the traditional use of units of nitrogen per hectare used in previous years. The treatments were performed on the surface of 25 hectares. The phases of intervention have been well planned and carried out according to the procedures in force in accordance with the timetable below: Period Application Products Method November 2008 Stubble STOPPIEZYM Spraying April 2009 Seeding CONCIAZYM Tanning May 2009 Foliar treatment IAGROENZYM Spraying June 2009 Foliar treatment IAGROENZYM Spraying 3 - CONDUCT TEST Treatment of stubble Treatment in the stubble (STOPPIEZYM) was conducted in November 2008 after harvest to facilitate the decomposition of the larvae and eggs of western corn rootworm. Plowing the vetch, clover, rape seed (legumes in general) before planting corn. So treated, the soil is enriched in its content of nitrogen, biomass and micro-organisms whose function is to nurture and make the soft and porous soil. 3.2 Seed treatment Seed treatment was performed in April 2009 in the dose of g CONCIAZYM out of 25,000 seeds, using a pneumatic drill.

6 Linea di Prodotti ECOENZIMI Foliar treatment Two foliar treatments were made from 5 / 6 leaf with 2 kg per hectare of IAGROENZYM in solution of water vaporized with about 30 units of nitrogen fertilizer per hectare (70% less than conventional dose between 75 to 130 units of fertilizer nitrogen per hectare) TEST RESULTS 4.1 Adjacent zone to the field operations of the company "Il Girasole" This land belonging to "Il Girasole" is divided by a country road just 3 m wide. Like the latter, a significant number of farms in the area were contaminated Rootworm and other pests like corn borer and soot. The collection of this type of plot not treated with the enzyme mixture has not been authorized.

7 Linea di Prodotti ECOENZIMI 4.2 Farm "Il Girasole The farm "Il Girasole", showed that treatment with products ECOENZIMI there were no evidence of contamination by rootworm and other pests. 7 It 'very interesting to note and emphasize that enzyme treatment of maize crops in the field of the farm "Il Girasole" enabled: inducing an auto immune defense of crops To avoid contamination of the environment and adjacent crops To prevent the contamination of adjacent crops to propagate within. Some statistics to conclude this chapter: The farm "Il Girasole" has had a collection with a yield of: 180 quintals per hectare on average over the 25 hectares covered. 220 quintals per hectare on a parcel of 5 acres. ear of an average weight between 480 and 580 grams ear of an average length between 25 and 28 centimeters

8 Linea di Prodotti ECOENZIMI CONCLUSIONS The harvest took place in late September 2009 and the results of regional and farm the plot "The Sunflower" can be summarized as follows: 5.1 Regional companies: nearby farms (3m): harvest prohibited parasitic contamination Regional companies: quintals per hectare without enzyme treatment 5.2 Farm "Il Girasole" Area covered: 25 hectares Average Yield per hectare in 2009: 180 kilos per hectare. Peak Performance in 2009: 220 kilos per hectare on a particle of the company

9 Linea di Prodotti ECOENZIMI average return in : tons per hectare without enzyme treatment Beyond these figures, which represent a clear and positive response to the expectations, these findings become the assumptions of reaction and behavior of enzyme products, in an experimental reality and production. 9 The same results were obtained in other types of agricultural crops (vines, vegetables, trees, potatoes, etc.....) and animal husbandry, with products and enzyme assays specifically designed for. but back to our "Corn" farm "Il Girasole" The results achieved, the product quality and quantity of the crop this year 2009, were very interesting and encouraging. The fact is all the more remarkable given the low environmental impact of operations, and the significant reduction in the amount of chemical fertilizers used. In the foregoing, do not forget to remember that the treatments were carried out in poor weather conditions of rain and the presence in the region of strong attacks rootworm (Diabrotica virgifera). Definitely a technique of soil tillage (plowed) and fertilizer residues preceding helped to achieve results above presented, but we are sure that everything is not dictated by the case: the correct use of enzyme products and farming techniques have given their significant contribution to the success of treatment. In 2009, the average production of 180 quintals per hectare of the farm "The Sunflower" (almost 2.5 times the average for the region) has been obtained by the use of mixtures of enzymes that are: Increase of microbial flora of soil by seed treatment (CONCIAZYM) The rapid germination and root system fortified (CONCIAZYM) It has promoted the development of corn plants with a high germination

10 Linea di Prodotti ECOENZIMI It has given to the plants mature a high immune to attacks of insects and parasitic fungi due to foliar treatments (IAGROENZYM) It has allowed the total development of plants to unfavorable weather conditions and criticisms of has reduced by 70% the use of nitrogen fertilizers (35 units instead of 130 units normally used) All areas adjacent to the farm "Il Girasole" and the entire Piedmont Region have been affected in 2009, the broad and deep rootworm (Diabrotica virgifera). The farm "Il Girasole" and other areas have undergone enzyme treatment, no statistically significant reported losses related to parasitic infections. The above is the period of experimental implementation, whatever the results, and established a program to use a standards-based enzyme mixtures, whose objectives are proposed below Objectives for the agricultural year and subsequent years. As part of the planned measures on farm "Il Girasole" and interventions from other companies to provide in crops of maize: Stabilize the output on the basis of the experimental results and reduce until the death of nitrogen fertilizers Treat the earth with the technique of enzyme STOPPIENZYM plowed with the mixture of corn stubble and the same fertilized with the technique of using green manure HAIRY (Vicia villosa) white mustard (Senapis Alba), barley and spray with the product STOPPIENZYM Practicing the technique of "false sowing", which is the preparation of the soil as if it were actually sown, followed by a mechanical intervention to remove the contaminated plants advance of planting time to encourage flowering before the appearance of any adult insects of Diabrotica in roots of maize (Diabrotica virgifera) treatments that aim to develop the root system (CONCIAZYM) and foliar treatments to feed the plants (IAGROENZYM) and increase their resistance to attack from parasites Refer to the bibliography of each term comparison of the physical characteristics of enzymes and their properties with the experimental results of this note.