Received: 20 th Mar-2013 Revised: 28 th Mar-2013 Accepted: 29 th Mar-2013 Research article

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Received: 20 th Mar-2013 Revised: 28 th Mar-2013 Accepted: 29 th Mar-2013 Research article BREEDING OF NEW POTENTIAL CROSSBREED (Bombyx mori L.), MSO3 x APS45 For TROPICAL REGIONS S.V. Seshagiri, J.

1 Received: 20 th Mar-2013 Revised: 28 th Mar-2013 Accepted: 29 th Mar-2013 Research article BREEDING OF NEW POTENTIAL CROSSBREED (Bombyx mori L.), MSO3 x APS45 For TROPICAL REGIONS S.V. Seshagiri, J. Seetharamulu, H. Lakshmi, S. Sankar Naik* and P.J. Raju Andhra Pradesh State Sericulture Research and Development Institute, Kirikera , Hindupur, Andhra Pradesh, India. *Dept. of Sericulture, Sri Krishnadevaraya University, Anantapur , Andhra Pradesh, India. seshu_apssrdi@rediffmail.com ABSTRACT: Keeping the various objectives of the industry in view, silkworm breeders are contributed significantly for the development of new strains through inbreeding coupled with appropriate selection procedures. Continuous renewal and change of existing breeds/hybrids with superior varieties and their commercialization is the need of the hour to increase silk quality and quantity. In this direction an attempt has been made at Andhra Pradesh State Sericulture Research and Development Institute (APSSRDI), Hindupur, India and contributed for the development of potential cross breed, MSO3 x APS45. Twenty four hybrid combinations were prepared in Line x Tester method involving eight newly developed polyvoltine lines with the three testers. The relative merit of the hybrids over multiple traits was assessed adopting Evaluation Index method. Based on the evaluation methods and performance, the hybrid MSO3 x APS45 were adjudicated as promising and chosen for further laboratory evaluation during different seasons. The performance data obtained was further subjected for multiple trait evaluation index method and the ranks were adjudicated based on the index values. The hybrid MSO3 x APS45 performed well under diversified environmental conditions indicating their overall stability and superiority which could be exploited at field level for the benefit of the sericulture farmers. Key words: Silkworm, breeding, crossbreed, heterosis, percent improvement, conventional method. INTRODUCTION The recent trend of global silk production centered mainly in tropical countries. India is the second largest producer of silk in the world next to China and more than ninety percent of the silk produced mainly by cross breeds (polyvoltine x bivoltine). To face the global competitiveness in silk production there is a need to develop more productive breeds or hybrids with quantitative and qualitative merit. The silkworm, Bombyx mori L. is a lepidopteran economic insect known for the production of mulberry silk which is aptly named as the Queen of Natural Fibers and has been successfully retaining its esteemed position even in the face of growing competition from other man-made fibers. Enrichment of silkworm breeds has always been one of the important factors contributing to increase in productivity in sericulture. Continuous renewal and change of existing breeds/hybrids with new superior varieties and their commercialization is the need of the hour to increase silk quality and quantity. During the early and later part of 1960, several attempts made by Indian breeders to replace the indigenous polyvoltine races resulted in the isolation of improved races. However, these races were not exploited due to their non-acceptance in the field due to certain negative parameters. Of late, the approach of breeding new productive breeds by genetic manipulation through conventional breeding has emerged as one of the powerful tools in exploiting the commercial qualities. The systematic hybridization coupled with appropriate selection procedures have contributed to amalgamate the major economic traits of choice from selected breeds and to synthesize genotypes of desirable genetic constitution [24]. The attempts to evolve highly productive bivoltine races have met with little success [16]. Hence, prominent breeders and geneticists of Japan [14,26] stressed the importance of polyvoltine breeding in the tropical regions of India. The silkworm, Bombyx mori L. offers one of the very important insects of choice with large number of strains which is best exemplified for utilization of heterosis by crossing them in different combinations [4]. In fact, silkworm is the only exception crop where hybrids are invariably used [32]. International Journal of Plant, Animal and Environmental Sciences Page: 222

2 The systematic rearing of F1 hybrids was undertaken for the first time in Japan after establishing the superiority of hybrids [27]. The final results in silkworm breeding are judged by the superiority on the commercial traits of the parental races that appear in F1. The advantages of rearing F1 hybrids are that they exhibit shorter larval duration, lower mortality, higher cocoon and shell weight and longer filament length [10]. A new era came into existence with the exploitation of heterosis which has increased the productivity of silk contributing to the development and expansion of sericulture industry. The combining ability analysis is a tool, widely used to determine the magnitude of heterosis and to identify perspective parents or hybrids for commercial exploitation of the hybrid vigor in agriculture, animal husbandry and poultry [23, 11]. An extensive study on the combining ability of silkworm was made to identify the superior and promising hybrid combinations for commercial exploitation in polyvoltine x bivoltine hybrids [2, 29, 18, 19, 22]. However, there is further scope in the improvement of genetic traits and identification of superior hybrids through estimation of heterosis by combining ability studies. Very limited number of polyvoltine cross breeds are available with all desired traits, which are not sufficient to meet the demand. At this juncture, there is a need for the development of quantitatively and qualitatively superior polyvoltine breeds and hybrids with high genetic plasticity to cater various climatic conditions. MATERIALS AND METHODS Two polyvoltine silkworm breeds viz., APMG4 and APMG16 maintained at Andhra Pradesh State Sericulture Research and Development Institute (APSSRDI), Hindupur constitute the parental material for the development of the breed MSO3. The breeding plan was initiated with the crossing of APMG4 and APMG16. The resultant line MSO3 was developed with qualitatively and quantitatively superior traits and subjected for hybrid testing by employing the Line x Tester analysis method along with other 7 newly evolved breeds with productively superior bivoltine breeds such as APS12, APS45 and APS8. The data pertaining to eight economic traits viz., fecundity, cocoon yield per 10,000 larvae by number, cocoon yield per 10,000 larvae by weight (kg), survival rate (%), cocoon weight (g), cocoon shell weight (g), cocoon shell ratio (%) and filament length (m) were analyzed for various hybrid combinations and the superiority of new polyvoltine hybrid MSO3 x APS45 was established when compared with other newly hybrid combinations. The merit of the each of the hybrid combinations was analyzed through multiple trait evaluation index method [13] as detailed below. Multiple Trait Evaluation Index Method Evaluation index over multiple traits was calculated as per the following formula which is suggested by Mano et al., A - B Evaluation Index = x C Where, A = Value obtained for a trait in a race B = Overall mean value of a particular trait of all the races C = Standard deviation of a trait of all the breeds 10 = Standard unit 50 = Fixed value Breeding and Selection Methods Emphasis was laid to select the cocoons of intermediate size and no selection pressure was applied during F1 F3 to retain gene pool responsible for different economic traits. Cellular rearing was carried out from F4 generation with minimum five replications. At the time of brushing, emphasis was given to high fecundity and good hatching percentage at every generation. The selected cocoons were subjected for sex separation to assess the cocoon, shell weight and cocoon shell ratio. This method of individual selection was followed to facilitate the fixation of desired economic traits at a faster rate. To select the parents at every generation, selection pressure was applied at every stage of development viz., egg, larva, pupa / cocoon and moth. International Journal of Plant, Animal and Environmental Sciences Page: 223

3 Development of Breed MSO3 The breeds APMG4 (females) and APMG16 (males) were used as initial parents for this plan. The F 1 progenies were raised by brushing composite layings and mass rearing was carried up to F 3 followed by cellular rearings. The cocoons were categorized into two types i.e., medium and elongated oval based on cocoon shape and breeding was continued by selecting medium oval type of cocoon. The population which showed uniformity in cocoon shape and consistent in the economic characters by F 12 generation was considered as fixed and it was named as MSO3. Fig. 1. Silkworm Larvae and cocoons of MSO3 Characteristics and Generation wise rearing performance of MSO3 The newly evolved polyvoltine silkworm line MSO3 which is characterized by bluish white larvae, plain and spinning green medium oval cocoons with medium grains. The generation wise rearing performance of the breed is as follows. Table 1. Generation wise rearing performance of MSO3 Generation Yield /10,000 Survival Cocoon Filament Larvae (No.) Rate (%) Weight (g) Weight (g) Ratio (%) Length (m) No. Wt.(kg) F F F F F F F F F F F F Average S.D CV (%) Characteristics of APS 45 The potential bivoltine breed APS45 was utilized for the development of the hybrid and the larvae of the breed is characterized by plain and bluish white in colour, cocoons are white and oval in shape with medium grains. The total larval period is days (Fig.3). International Journal of Plant, Animal and Environmental Sciences Page: 224

4 APMG4 X APMG16 F 1 F 2 Medium & Elongated oval F 3 F 4 F 5 F 6 F 7 F 8 F 9 F 10 F 11 F 12 MSO3 = Mass selected = Cellular selected = Cellular non-selected Fig.2. Breeding plan of MSO3 International Journal of Plant, Animal and Environmental Sciences Page: 225

5 Fig. 3. Larvae and cocoons of APS45 Hybrid evaluation The newly developed line MSO3 along with other seven evolved polyvoltine breeds viz., MSO1, MSO2, MSO4, MSO5, MSO6, MSO7 and MSO8 as lines and productively superior bivoltine breeds such as APS12, APS45 and APS8 as testers were subjected for hybrid evaluation study following the Line x Tester analysis method. A total of twenty four new hybrid combinations were prepared and reared in replications by following the standard rearing techniques as per the procedure suggested by [10]. The data pertaining the major silk contributing traits. viz., fecundity, cocoon yield per 10,000 larvae by number, cocoon yield per 10,000 larvae by weight (kg), survival rate (%), cocoon weight (g), cocoon shell weight (g), cocoon shell ratio (%) and filament length (m) were recorded and the superiority of the hybrid MSO3 x APS45 was established with the average evaluation index value Characteristics of MSO3 x APS45 The larvae are bluish white in colour and cocoons are greenish yellow with medium grains. The larval period varied between days. The hybrid (Fig. 4) showed overall merit for quantitative and qualitative traits compared to the control hybrids viz., APM1 x APS8 and PM x CSR2. Fig.4. Silkworm larvae and cocoons of MSO3 x APS45 (Evolved hybrid value Control hybrid value) Percent improvement= X 100 Control hybrid value F1 Mid Parent Value (MPV) Heterosis % (MPH) = X 100 Mid Parent Value RESULTS The rearing performance during breeding of the line MSO3 is presented in Table 1. Perusal of the data revealed that the fecundity recorded an overall mean of 498 and among the generations it was varied between 540 (F 2 ) and 477 (F 11 ). Further, the trait that showed variations in early (F 2 F 5 ) and middle generations (F 6 F 10 ) followed by consistency in the later generations (F 11 to F 12 ) which indicates the fixation of the line for the trait. With regard to cocoon yield by number, it was varied between 9167 (F 11 ) to 9500 (F 4 ) with an average of 9311, SD of 95 and CV of 1.02 %. International Journal of Plant, Animal and Environmental Sciences Page: 226

6 The line MSO3 at F 1 was found with kg and kg at F 12 with an overall mean of kg for the trait cocoon yield per 10,000 larvae by weight and the maximum variability of the trait is evidenced from higher CV of 5.48 %. Over the twelve generations, the trait was ranged to the maximum of % (F 6 ) and to the minimum of % (F 2 ) and minimum variability of the trait is evidenced from lesser CV of 0.95 % for the trait survival rate. However for the various traits studied showed consistency in the later generations that suggests the fixation of the trait. Hybrid Testing Mean rearing performance data of the 24 new hybrid combinations and Evaluation index values (EI) is presented in Table 2 and the hybrid MSO3 x APS45 showed economic merit for the traits studied. The Percent improvement in economic characters in comparison to control hybrids and Hybrid vigour of MSO3 x APS45 is presented in the Table 3. Table 2. Mean Performance of new hybrids Sl. Yield /10,000 Pupatio Cocoon Filament EI No Combination larvae (No.) n (%) weight (g) weight (g) ratio(%) length(m) value. No. Wt.(kg) 1 MSO1 x APS MSO1 x APS MSO1 x APS MSO2 x APS MSO2 x APS MSO2 x APS MSO3 x APS MSO3 x APS MSO3 x APS MSO4 x APS MSO4 x APS MSO4 x APS MSO5 x APS MSO5 x APS MSO5 x APS MSO6 x APS MSO6 x APS MSO6 x APS MSO7 x APS MSO7 x APS MSO7 x APS MSO8 xa PS MSO8 x APS MSO8 xaps Average SD CV(%) Combination Table 3. Percent improvement in economic characters of MSO3 x APS45 Yield /10,000 larvae (No.) No. Wt. (kg) Pupation (%) Cocoon weight (g) weight (g) ratio (%) Filament length(m) MSO3 x APS PM x CSR2 (Control) Percent improvement International Journal of Plant, Animal and Environmental Sciences Page: 227

7 Table 4. Hybrid vigour in MSO3 x APS45 Combination Yield /10,000 Cocoon Filament larvae Pupation weight weight length (No.) (%) ratio(%) No. Wt. (kg) (g) (g) (m) MSO APS Average MSO3 x APS MPH Season Table 5. Seasonal performance of MSO3 x APS45 Yield / 10,000 Larvae No. Wt. (Kg) Survival Rate (%) Cocoon Weight (g) Weight (g) Ratio (%) Monsoon Post monsoon Pre monsoon Average The hybrid MSO3 x APS45 (69.37) with highest EI over multiple traits excelled over other combinations. Further the combination recorded higher percent improvement over its control for all the 8 traits studied with highest (14.95%) improvement was recorded for the trait shell weight. The hybrid also exhibited positive heterosis to a varying degree (Table 4) for different traits ranged to a maximum of 26.44% (Yield/10000 larvae by wt. (kg)). The performance of the hybrid in various seasons is presented in Table 5. DISCUSSION The silkworm breeding is the most important example where heterosis is being exploited commercially to the maximum extent. The conventional breeding methods adopted in silkworm breeding over the years have resulted in the larger number of inbred lines with distinct genotypic and phenotypic differences. Various systematic procedures have enabled the breeders to identify the best combiners and their combining ability in specific hybrid combinations. In view of this, the ultimate result in silkworm breeding is judged by the excellency of characters of the parental strains that appear in F 1. During the course of evaluation, it is essential to understand the nature of allelic differences between the pure lines as well as their phenotypic manifestation in heterozygous condition [31]. Moreover, the evaluation of general combining ability of the pure lines and the specific combining ability of the hybrids will be of utmost use in interpreting the genetic basis of phenotypic manifestation of most of the characters [5,8]. The superiority of the hybrids over parental strains is undoubtedly due to variable magnitude of heterosis for the quantitative characters in silkworm and the results of present study are corroborating the findings of [6]. It has been pointed out by [28] that the F1 hybrids in silkworm, Bombyx mori L. in several aspects are superior to their pure line parents and the present results are in support with the findings of [9, 32]. To achieve desired goals, cross breeding is widely used in commercial animal production as a means of exploiting heterosis [1, 20]. Several attempts were made in India by eminent silkworm breeders to identify suitable hybrid combinations nevertheless there is an enormous requirement for development of productively and qualitatively superior hybrids [3]. For this, it is necessary to test large number of hybrid combinations to identify the pure races which can be utilized for ultimate identification of the superior hybrid combinations. In view of the above, the present investigation was made to identify the superior hybrid combinations by utilizing the eight newly evolved polyvoltine lines and three productively superior bivoltine breeds as testers by employing Line x Tester method. International Journal of Plant, Animal and Environmental Sciences Page: 228

8 The parental lines possessing high general combining ability (gca) are preferred for population development and for initiation of pedigree breeding as it is heritable which can be fixed. The wider variability observed for important economic traits in the hybrids can be attributed to the genetic diversity present among the newly developed lines utilized in hybrids. The magnitude of specific combining ability (sca) is an important aspect to be evaluated in the hybrids to estimate their superiority. In the present investigation the sca effects varied considerably among F 1 hybrids. The identification of superior hybrids based on sca effects are in agreement with the works of [7, 12, 25]. The exploitation of heterosis is an important step towards achieving desirable economic effects from the hybrids. The combination MSO3 x APS45 recorded highly significant desirable mid parent and better parent heterosis and the present findings are in agreement with [15]. The hybrids derived in the present study showed positive heterosis for most of the traits and overdominance for only some traits which corroborate the findings of [16,21] which is ascribed to the allelic interaction. The multiple trait evaluation of the twenty four hybrid combinations revealed that, eight combinations which recorded average cumulative index values above 50 possess economic merit. Among them, the combination MSO3 x APS45 ranked first followed by MSO1 x APS12. These observations confirm the established fact as observed by [17, 30] that superiority of one or a couple of characters may not reflect the overall merit of the hybrid. Since the comprehensive merit of the hybrid over a range of traits depends on relative superiority of many individual traits, selection needs to be based on multiple traits contributing to overall silk output. Based on the performance, heterosis, overdominance and evaluation index, the hybrid MSO3 x APS45 is adjudicated as most promising for commercial exploitation. REFERENCES [1] Bowman, J.C Selection for heterosis. Anim. Breed. Abst. 27: [2] Chouhan, T.P.S., Lakshmanan, V. and Rajalakshmi, E Combining ability studies in bivoltine silkworm, Bombyx mori L. Indian J. Seric. 39: [3] Dandin, S. B., Ravindra Singh, Ragavendra Rao, D., Basavaraja, H. K. and Kariappa, B. K Studies on the isolation of promising multivoltine breed ND7 and its F1 hybrid performance with productive bivoltine races. Indian J. Seric., 46(1): [4] Datta, R.K. and Nagaraju, J Genetic engineering and tropical sericulture. Indian Silk 26(3): [5] Falconer, D.S Introduction to quantitative genetics. Oliver and Boyd, Edinburgh, U.K. [6] Gamo, T Recent concepts and trend in silkworm breeding. Farming Japan 10: [7] Goel, A.K Breeding for the development of inbred lines and their heterotic effects on economic traits of silkworm, (Bombyx mori L.), Ph.D. thesis, University of Mysore, Mysore. [8] Griffing, B. and Zsiros, Z Heterosis associated with genotype environment interactions. Genetics 68: [9] Harada, C On the heterosis of quantitative characters in silkworm. Bull. Seric. Expt. stn. 17, [10] Krishnaswami, S., Narasimhann, M.N., Suryanarayana, S.K. and Kumararaj, S Silkworm rearing In: Manual on Sericulture Vol. 2, UN Food and Agriculture Organization, Rome, [11] Kurian, A. and Peter, K.V Line x Tester analysis for yield and processing characteristics in tomato. J. Trop. Agric.33: [12] Malik, G.N., Masoodi, M.A., Kamili, A.S. and Sofi, A.M Studies on heterosis in some bivoltine silkworm (Bombyx mori L.) crosses. J. Seric. 6(1&2): [13] Mano, Y., Nirmal Kumar, S., Basavaraja, H.K., Mal Reddy, N. and Datta, R.K A new method to select promising silkworm breeds/combinations. Indian Silk 31(10): 53. [14] Murakami, A Ecogenetical studies on tropical mulberry silkworm Bombyx mori. Proceedings of the Intl. Cong. on Trop. Seri. Pract. Bangalore, India. pp [15] Nanjundaswamy, L Studies on the combining ability in silkworm, Bombyx mori L. Ph.D Thesis, University of Mysore, Mysore, India. [16] Raju, P.J Studies on the hybridization and synthesis of new breeds of silkworm, Bombyx mori L. Ph.D. Thesis, University of Mysore, Mysore. [17] Ramesh Babu, M., Chandrashekharaiah, Lakshmi, H. and Prasad, J Multiple trait evaluation of bivoltine hybrids of silkworm (Bombyx mori L.). Int. J. Indust. Entomol. 5(1): International Journal of Plant, Animal and Environmental Sciences Page: 229

9 [18] Rao, C.G.P., Chandrashekharaiah, Ibrahim Basha, K., Seshagiri, S.V., Ramesh, C. and Nagaraju, H Identification of superior polyvoltine hybrids (Poly x bivoltine) of silkworm, Bombyx mori L. Int. J. Indust. Entomon. 8, [19] Ravindra Singh, Sharma, S.D., Raghavendra Rao, D., Chandrashekaran, K., Basavaraja, H.K. and Kariappa, B.K Line x Tester and heterosis analysis in silkworm, Bombyx mori L. Indian J. Seric. 44(1): [20] Sang, J.H Hybrid vigour and animal production. Anim. Breed. Abst: 24(1): 1-6. [21] Satenahalli, S.B., Govindan, R. and Goud, J.V Heterosis studies for some quantitative traits in silkworm, Bombyx mori L. Indian J. Seric. 28(1): [22] Seshagiri, S.V., Ramesha, C. and Rao, C.G.P Genetic manifestation of hybrid vigor in cross breeds of mulberry silkworm, Bombyx mori L. Int. J. Zoological Research. ISSN (Academic Journals Inc. alert.com). [23] Sprague, G.F. and Tatum, L.A General vs. specific combining ability in single crosses of corn. J. Amer. Soc. Agron. 34: [24] Sreerama Reddy, G., Raju, P.J. and Maribhasetty, V.G Heterosis and its application in silkworm, Bombyx mori L. Indian Sec. Life. Sci. Manas pub. Kanpur, Inida. pp [25] Sudhakara Rao, P., Datta. R.K., Basavaraja, H.K., Vijayakumari, K. M. and Rekha, M Evaluation of combining ability for certain quantitative traits through diallel crosses in the silkworm, Bombyx mori L. Indian. J. Seric. 44(1): [26] Tazima, Y Report on sericulture industry in India. Central Silk Board, Bombay, India. pp [27] Toyama, K Sericulture in Siam. Dainihon Sanshi Keinko. pp [28] Toyama, K Breeding methods of silkworm. Sangyo Shimpo. 158: [29] Vasileva, Y., Petkov, N., Petkov, Z., Matef, A., Marghitas, L. and Dinita, G Combining ability evaluation of perspective Bulgarian and Romanian silkworm, (Bombyx mori L.) races. Bulgarian J. Agric. Sci. 10(2): [30] Vidyunmala, S., Narasimha Murthy, B. and Sivarami Reddy, N Evaluation of new mulberry silkworm (Bombyx mori L.) hybrids (multivoltine x bivoltine) through multiple trait evaluation index. J. Ent. Res. 22(1): [31] Whaley, W.G Physiology of gene action in hybrids. In: Heterosis Ed. By J. W. Gowen Hofner Publ. NY. [32] Yokoyama, T Silkworm selection and hybridization. In: Genetics in relation to insect management. Working Papers. The Rockfeller Foundation Management. pp International Journal of Plant, Animal and Environmental Sciences Page: 230

IDENTIFICATION OF BIVOLTINE BREEDS AND HYBRIDS OF THE MULBERRY SILKWORM, BOMBYX MORI L. Ravindra Singh* and D. Gangopadhyay

203 IDENTIFICATION OF BIVOLTINE BREEDS AND HYBRIDS OF THE MULBERRY SILKWORM, BOMBYX MORI L. Ravindra Singh* and D. Gangopadhyay *Silkworm Seed Technology Laboratory, NSSO, Kodathi, Bangalore - 560 035,