Biopesticide test of neem seed (Azadirachta indica A. Juss.) extract and MaviNPV virus for the control of main insects pest of cowpea in Niger

Transcription

1 Biopesticide test of neem seed (Azdircht indic A. Juss.) extrct nd MviNPV virus for the control of min insects pest of cowpe in Niger Oussein ABDOULAYE ZAKARI, Irhim BAOUA, Seyni BOUREIMA, Mnuele TAMÒ, Sdou MAHAMANE, Brry Roert PITTENDRIGH Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

2 Introduction Cowpe Vign unguicult Wlp is the min food legume world-wide (Singh et l.. 2); Niger is the 2 nd lrgest cowpe producing country with n verge production of 1.7million tons over n re of 55 h (MA, 215); However Yield remin still low in the min production res nd does not exceed 297 to 332 kg / h (MA, 215). Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

3 Environment Diseses Cowpe Climtic conditions Weeds Insects (Singh et l. (1979) ;Rchi (1985) Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

4 Pests Very expensive; Environmentl nd helth Hzrds. Pesticides Bio pesticides Effective; Environmentl friendly; Aville loclly (Silv-Agyo 213) Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

5 Methods Ojective: This study ws conducted to test the effectiveness of neem seed extrct nd the MviNPV virus for the control of cowpe pests. Experimentl tril: Fisher lock design 6 tretments ; 8 repetitions. The experimentl plots re 3m 2 ; nd The cowpe vriety used ws IT9K with density of 75 x 35 cm Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

6 Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

7 The tretments: 1. Neem seeds queous extrct ( 12.5kg/h) 2. Neem oil (1,6l/h) 3. MviNPV virus (115ml/h) Neem seeds 4. TopBio (2l/h) + MviNPV virus 5. Pesticide : Conquest C 88 EC ( 5ml/h; Acetmiprid 16g /l,cypermethrin 72g/l) 6. Control ( 4 pplictions) Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

8 Dt collected o Pests infesttions ; o Pests dmge on cowpe s flowers nd pods ; o Seeds s qulity evlution through determining dmged seeds y LPB,PSB nd others pests per tretment; o Yield per tretments fter hrvest. Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

9 Dt collection Mrked stnd Flowers nd pods collection Plstic vils Dissection nd lrve counting Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

10 . RESULTS

11 figure 1: Pest proportion oserved on cowpe crop in Mrdi sttion Pesticide Neem seeds extrct TopBio + MviMNPV virus Men numer of individul/tretment Neem oil MviMNPV virus Control LPB PSB M. sjostedti Cowpe growing seson c Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

12 Figure 2: Proportion of Flowers nd pods dmged y LPB nd PSB Pesticide Neem oil Neem seeds extrct MviMNPV virus TopBio + MviMNPV virus Control Proportion dmged cowpe orgn / seson 1. LPB Flowers Pods c PSB Cowpe growing seson c c c c Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

13 Pest dmges on cowpe pods Dmged pods y M.vitrt Dmged pod y C.tomentosicollis Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

14 Figure 3: Proportion of seeds dmged y LPB,PSB nd others pests Pesticide Neem oil Neem seeds extrct MviMNPV virus TopBio + MviMNPV virus Control Helthy seeds 1 5 c c cd d 1 5 c cd d LPB dmged seeds 4 2 c 4 2 c PSB dmged seeds Others pests dmged seeds Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

15 Dmged seeds Helthy seeds PSB dmged seeds LPB dmged seeds Others pests dmged seeds Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

16 Tle 1: Yield kg / h per tretment Tretement Pesticide 143± ± ±77 Neem seeds extrct 1241±35 1± ±15 Neem oil 542±12 c 842± ±76 MviNPV virus 724±58 c 791± ±62 TopBio + MviNPV virus 574±28 c 691± ±7 Control 154±42 c 2±72 c 11.16±5 c ANOVA F=8.16; P<.1 F=11.4; P=.3 F=16.35; P=.1 Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

17 Conclusion The study found tht the use of iopesticides llows n increse in yield of 323%; The neem seeds extrct t dose of 5% provides n verge cowpe yield of 985 kg / h ; n increse in yield of 679% ; The use of neem seed extrct ville in the country is to promote on-frm; Phytosnitry tretment must strt erly to ensure etter crop protection. Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

18 Acknowledgements Feed the Future Innovtion L for Collortive Reserch on Grin Legumes

19 Feed the Future Innovtion L for Collortive Reserch on Grin Legumes