TABLE OF CONTENTS. Abbreviations List of figures List of Tables Acknowledgements Abstract..1-2

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1 TABLE OF CONTENTS Abbreviations List of figures List of Tables Acknowledgements Abstract..1-2 Chapter 1: Introduction and Review of literature Introduction: Wheat Economic importance and taxonomy of the wheat Wheat cultivation in India 1.2 Water stress 1.3. Drought tolerance Drought Mechanism of drought tolerance Drought escape Dehydration avoidance Dehydration tolerance A conceptual model for drought tolerance 1.4 Identification of the physiological traits for use as a selection criterion 1.5 Carbon Isotope Discrimination Sample selection criterion Carbon isotope discrimination (High or low) Optimal yield conditions 1.6 Surrogates 1.7 Ash content: Determination of ash content Choice of environment and type of sample 1.8 Canopy temperature depression (CTD) Factors affecting expression of CTD Combining selection for CTD and leaf conductance 1.9 Water Use Efficiency (WUE) 1.10 Molecular mapping in plants

2 Molecular marker technologies for genetic mapping 1.11 Progress in wheat molecular genetics Use of molecular markers for mapping and gene identification 1.12 Mapping populations 1.13 Mapping QTLs in wheat 1.14 Genesis and structure of the thesis Chapter 2: Utilization of carbon isotope discrimination and ash content to select wheat under water stress condition Introduction 2.2 Material and Methods: Selection of wheat varieties and procurement of seeds Experimental Trials Climatic conditions Observations Agronomical traits Physiological traits 2.3 Statistical analysis 2.4 Results 2.5 Discussion Effect of water treatment on grain yield, and m a Relationship between grain yield and morphological and phenological traits Relationship between grain yield, and m a under RSMS Relationship between grain yield, and m a under PAWS Relationship between grain yield, and ma under WW conditions Differences between aestivum and durum wheat cultivars Chapter 3: Relationship between carbon isotope discrimination, ash Content, canopy emperature depression and grain yield in wheat under water stress condition PART A: DURUM WHEAT 3.1A Introduction

3 3.2 A Materials and Methods 3.2.1A Selection of wheat varieties and procurement of seed 3.2.2A Experimental Trials 3.2.3A Climatic conditions: A Observations 3.3A Statistical analysis 3.4A Results 3.4.1A Effect of water regime on grain yield, carbon isotope discrimination and ash content 3.4.2A Relationship between grain yield, carbon isotope discrimination, canopy temperature depression and ash content under residual soil moisture stress condition: 3.4.3A Relationship between grain yield, carbon isotope discrimination, canopy temperature depression and ash content under post anthesis water stress condition 3.4.4A Relationship between grain yield, carbon isotope discrimination, canopy temperature depression and ash content under well watered condition 3.5A Discussion: 3.5.1A Effect of water treatment on Grain yield, and m a 3.5.2A Relationship between GY,, CTD and m a under residual soil moisture stress 3.5.3A Relationship between GY,, CTD and m a under post anthesis water stress (PAWS) condition 3.5.4A Relationship between GY,, CTD and m a under well watered (WW) conditions PART B: AESTIVUM WHEAT 3.1 B: Selection of wheat varieties and procurement of seed 3.2 B Experimental conditions 3.3 B Measurements 3.4 B Statistical analysis 3.5 B Results B Effect of water regime on grain yield, ash content and CID

4 3.5.2 B Relationship between grain yield, canopy temperature depression (CTD) and ash content under PAWS condition B Relationship between grain yield, CTD and m a under well watered condition 3.6 B Discussion B Relationship between grain yield, morphological and phenological traits B Relationship between grain yield, CTD, and m a under PAWS conditions B Relationship between grain yield, canopy temperature depression, carbon isotope discrimination and ash content under WW conditions Chapter 4: Analysis of the relationship between carbon isotope discrimination, ash content, grain yield and water use efficiency components in durum wheat Introduction 4.2 Materials and methods: Plant material Experimental conditions Observations Estimation of soil water balance and water use efficiency components 4.3 Statistical analysis 4.4 Results 4.5 Discussion Relationships between grain yield, and m a under different water regimes Variation of WUE components with water regimes Relationship between, m a and water use efficiency components across environments Chapter 5: Identification of QTLs for carbon isotope discrimination, ash content and canopy temperature depression Introduction 5.2 Material and methods: Plant material:

5 5.2.2 Experimental conditions: Phenotypic analysis 5.3 Measurements: Carbon isotope Discrimination (CID): Ash content 5.4 Statistical analysis: 5.5 QTL analysis 5.6 Results Identification of QTLs for Carbon Isotope Discrimination (CID), ash content and Canopy Temperature Depression (CTD): QTL for ash content in WW and PAWS condition QTL for CID in WW condition QTL for CTD in WW condition 5.7 Discussion Chapter 6: Summary and Conclusion Important research findings Identification of suitable physiological traits which can be useful in wheat breeding under water stress environment Analysis of the relationship between carbon isotope discrimination, ash content, grain yield and water use efficiency components in durum wheat Identification of QTLs for carbon isotope discrimination, ash content and canopy temperature depression Bibliography