2. TITLE OF PROPOSAL: Continuation of the Intensive Management of the State Variety Trials for Hard Red Spring Wheat.

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1 FOR ADMINISTRATIVE USE Program Area Code Proposal Code Minnesota Wheat Research and Promotion Council RESEARCH PROPOSAL GRANT APPLICATION 1. NAME AND ADDRESS OF ORGANIZATION TO WHICH AWARD SHOULD BE MADE Name: Address: Regents of the University of Minnesota Sponsored Projects Administration 200 Oak Street SE, Suite 450, Minneapolis, MN TITLE OF PROPOSAL: Continuation of the Intensive Management of the State Variety Trials for Hard Red Spring Wheat. 3. PRINCIPAL INVESTIGATOR(S) Jochum J. Wiersma PI# 2 Name: James A. Anderson 4. PI #1 BUSINESS ADDRESS Northwest Research & Outreach Center 2900 University Avenue Crookston, MN PI# 3 Name: 5. PROPOSED PROJECT DATES (calendar years) 1/1/ /31/2009 Note: Research Reports are Due November 15th of Each Year 6. TOTAL PROJECT COST $ PI #1 PHONE NO RESEARCH OBJECTIVES: (List objectives to be accomplished by research grant) The objectives of this research are to: 1) To continue testing of the state variety trials under both conventional and intensive production practices. 2) To report the results of both sets of trials in future editions of the Minnesota Variety Trials Bulletin Attach a 2-page detailed discussion of importance of the proposal to wheat profitability; how study complements previous research in area; procedures to be used; and competency of the research group in achieving research objectives. (Please keep the proposal concise, only 2 pages will be provided reviewers). Signature Of Principal Investigator Date Phone Number Signature Of Authorized Representative Title Date Address Of Authorized Representative Phone Number Kevin McKoskey, Branch Mgr., McNamara Bldg. Suite 450, 200 Oak St Minneapolis MN

2 Minnesota Wheat Research and Promotion Council RESEARCH PROJECT PROPOSAL (2-pages maximum) Project Title: Continuation of the Intensive Management of the State Variety Trials for Hard Red Spring Wheat. Importance: The last 5 years have clearly shown that significant rank changes occur for grain yield when the yield trials are treated with fungicides to prevent fungal pathogens affecting grain yield. These results are in line with the previously reported results in the literature (Guy et al. 1989; Jorgenson et al. 1994; Puppala et al. 1998, Wiersma and Motteberg 2005). This simple approach to test varieties under both the conventional and intensive management approach and to report both sets of yield data has been well received by growers. It is now an integral part of the State Variety Trials for Hard Red Spring Wheat (Table 1). We therefore propose to continue testing of the state variety trials under both management regimes. Background: The HRSW yield trials at the Research and Outreach Centers in Lamberton, Morris, Crookston, and Roseau have been expanded to include two identical, adjacent trials that differ in their pest management regime. The two management approaches are the standard system of conducting yield trials in which insects and fungal pathogens are not controlled and a second management approach that included the use of a fungicide at Feekes 5 in combination with fungicide treatments at Feekes 9 and as well as the use of an insecticide to control any insect pests. The two management approaches are labeled intensive and conventional. The previous years have shown that rank changes are common, and thus warrant publication as variety selection is often more than not based on the ranking of varieties. Relationship To Past Projects: This is a continuation of the grant titled Intensive Management of the State Variety Trials for Hard Red Spring Wheat, which was funded over the past 5 years. Procedures: We propose to continue the current yield trials at the Research and Outreach Centers in Lamberton, Morris, Crookston, and Roseau with two adjacent but separate yield trials with 3 replications. For the application at Feekes 5 Stratego (Bayer CropScience, Alexander Drive, Research Triangle Park, NC 27709, USA) - a mixture of 11.4% trifloxystrobin and 11.4% propiconazole will be used. Based on the recommendations for early season fungicide applications in the UK, one half of the labeled rate or 366 ml/ha of Stratego will be applied (Home Grown Cereal Authority, 2000). For the application at Feekes 9, the labeled rate of 292 ml/ha of Tilt (propiconazole) (Syngenta Crop Protection, Inc., P.O. Box 18300, Greensboro, NC 27419) will be used. For the fungicide application at Feekes 10.51, the labeled rate of 440 ml/ha of Prosaro (12.8% prothioconazole % tebuconazole) (Bayer CropScience, Alexander Drive, Research Triangle Park, NC 27709, USA) will be used. Fungicide applications will be made using a tractor mounted sprayer that is equipped with 8002 TwinJet nozzles (Spraying Systems Co., North Avenue, Wheaton, IL 60188, USA) delivering 150 L/ha at 275 kpa. Fungicides will be applied either in the morning or early evening to reduce drift. The timing of each application will be based on the growth stage of the individual plots. Aphids will be controlled with a labeled insecticide if the University of Minnesota threshold is exceeded. Plots will be harvested with a small plot combine. Harvested grain will be dried and cleaned with a Clipper office tester and cleaner (Seedburo Equipment Co., Chicago, IL 60607, USA). Grain yield and test weight will be expressed as bu/a and lbs/bu, respectively. Grain protein content will be determined on a 0.5 kg sub-sample by near infrared transmission using a Tecator Infratec 1229 Grain Analyzer (Foss North America, Inc., Eden Prairie, MN 55344, USA) following AACC method (American Association of Cereal Chemists 1995). Research Group: Jochum Wiersma, James Anderson, John Wiersma, Steve Quiring, George Nelson, James Cameron, Don Velleckson, Derek Crompton, and Gary Linkert. Regional Linkages To Other Research Activities: none Additional Sources of Funding: none References: American Association of Cereal Chemists Approved Methods of the AACC. American Association of Cereal Chemists, Inc. St. Paul, MN.

3 Beuerlein, J.E., Oplinger, E.S., and Reicosky, D Yield and agronomic characteristics of soft red winter wheat as influenced by management. J. Prod. Agric. 4: Guy, S.O., Oplinger, E.S., Wiersma, D.W., and Grau, C.R Agronomic and economic response of winter wheat to foliar fungicides. J. Prod. Agric. 2: Home Grown Cereal Authority (HGCA) Wheat disease management guide. HGCA, Caledonia House, London, UK. Jorgenson, L.N., Secher, B.J.M., and Welling, B Resistance in cereal cultivars and the need for fungicide treatments. Annals of Applied Biology 40: Puppala, V., Herman, T.J., Bockus, W.W., and Loughin, T.M Quality response of twelve hard red winter wheat cultivars to foliar fungicides across four locations in central Kansas. Cereal Chemistry 75: Wiersma, J.J., and C.D. Motteberg. Evaluation of five fungicide application timings for control of leaf-spot diseases and fusarium head blight in hard red spring wheat Can. J. Plant Pathol. 27: Appendix Table 1 Grain yield and ranking of individual cultivars across locations in multiple year comparisons ( ). North South State year 3 year year 3 year year 3 year Variety Conv Int Conv Int Conv Int Conv Int Conv Int Conv Int Conv Int Conv Int Conv Int Rush Ulen Kelby Briggs Trooper Samson Traverse Banton Glenn Oklee Granger Oxen Norwell Steele ND Freyr Howard Kuntz Alsen Knudson FBC Dylan Ada Blade RB Bigg Red Faller HatTrick Cromwell Granite Fireball Marshall Vantage Hotshot Polaris Bakker Gold Mean LSD (0.05) Env

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5 Minnesota Wheat Research and Promotion Council RESEARCH PROJECT PROPOSAL BUDGET ORGANIZATION AND ADDRESS Name: Address: Regents of the University of Minnesota Sponsored Projects Administration 450 McNamara Alumni Center, 200 Oak Street SE Minneapolis, MN Principal Investigator(s) / Project Directors(s) Funds Requested For Year 1 Year 2 Year 3 (2009) (2010) (2011) A. Salaries and Wages $ $ $ 1. Co-principal Investigator(s) 2. Senior Associates 3. Research Associates - Post Doctorate 4. Other Professionals 5. Graduate Students 6. Prebaccalaureate Students 7. Secretarial - Clerical 8. Technical, Shop and Other B. Fringe Benefits C. Nonexpendable Equipment (Planting and harvesting equipment use) $ D. Materials and Supplies $ E. Travel $500.- F. Publication Costs G. Computer Costs H. All Other Direct Costs (Attach supporting data) See Attached I. TOTAL AMOUNT OF THIS REQUEST (per year) $ $ $

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