Information Exchange Working Group. June 2009 June 2010

Transcription

1 Information Exchange Working Group June 2009 June 2010

2 ISTA Workshop on Variety Testing - an introduction, using protein electrophoresis & Polymerase Chain Reaction (PCR) for GMO Detection April 6 th 10 th, 2010 Bangalore, India

3 WE WOULD LIKE TO THANK THE FOLLOWING ORGANIZATIONS FOR THEIR SPONSERSHIP

4 Orgenizers & Lecturers: Dr. G. V. Jagadish Indo-American Hybrid Seeds Pvt Bangalore, India Prof. Dr. Norbert Leist Mr. Rainer Knoblauch Dr. Benjamin Kaufman Dr. Kirk Remund

5 DAY 3 THURSDAY Theory Short review of the day before, given by participants Theory Evaluation of results, IEF, MDH. Final discussion on variety testing, Knoblauch, Leist; until Saturday training in method development at the hosting laboratory Primer to Introduction to GMO testing & work-shop GMO overview - Kaufman Testing DAY 4 FRIDAY COFFEE BREAK Theory Short review of the day before, given by participants Theory Generalities and statistical GMO testing plans using qualitative assays - Remund Theory From Seed to DNA: DNA Extractions - Kaufman Theory Introduction to SeedCalc Remund Theory From Seed to DNA: DNA quantification and normalization LUNCH Kaufman Theory Statistical GMO testing plans COFFEE using quantitative BREAK assays -Remund Practice SeedCalc Class Exercises Remund Practice DNA Isolation - Kaufman COFFEE BREAK LUNCH Theory Getting a %GM Estimate and associated DAY 5 SATURDAY confidence limits - Remund Practice DNA visualization, quantification and normalization - Kaufman Practice SeedCalc Class Exercises - Remund Theory Short review of the day before, given by participants FINISH COFFEE BREAK Theory PCR for GMO testing - Kaufman Theory Introduction Practice to PCR - Kaufman Qualitative PCR set up - Kaufman FINISH COFFEE BREAK Theory Real-time PCR for GMO quantification - Kaufman Practice Real-time PCR set-up - Kaufman LUNCH Practice PCR result visualization & and documentation - Kaufman Theory ISTA and the GMO Task Force Activities to determine genetically modified seeds - Kaufman COFFEE BREAK Theory Review of results and WS conclusion Remund, Kaufman FINISH

6 20 participants 8 Government agencies, 7 Academic Research Inst.s, 5 Industry From: India, Pakistan, Kenya & Thailand

7 Biotechnology Trait Detection Workshop June 8-12, 2010 Oberschleißheim, Germany Organized by: Bavarian State Office for Health and Food Safety International Seed Testing Association GMO Task Force

8 WE WOULD LIKE TO THANK THE FOLLOWING ORGANIZATIONS FOR THEIR SPONSERSHIP:

9 Organizers & Lecturers: Mr. Ulrich Busch Bavarian Health and Food Safety Authority Oberschleißheim, Germany Mr. Benjamin (Beni) Kaufman BioDiagnostics Inc. River Falls, WI, USA Ms. Cheryl Dollard, MSc. Ottawa Plant Laboratory Canadian Food Inspection Agency Ottawa, Ontario, Canada Mr. Bruno Zaccomer Monsanto Peyrehorade, France Mr. Jean-Louis Laffont Pioneer Génétique Aussonne, France

10 19 Participants 9 Government agencies, 2 Academic Research Inst.s, 8 Industry Australia, Austria, Brazil, Bulgaria, Chile, France, India, Iraq, Ireland, Italy, Poland, Saudi-Arabia, Serbia, Spain

11 Mean rating to questions Statistics progress (after-before) Lab practices progress (after-before) My expectations were met overall Lecturers knowledge Lecturers enthousiasm Lecturers preparation Organization Materials were effective and helped me to content relevant to my job Statistics knowledge after Lab practices knowledge after Statistics knowledge before Lab practices knowledge before Rating 1 Poor 2 Marginal 3 Fair 4 Good 5 Very good 6 Excellent nc No comment blk No response

12 Suggestions for changes for improvment by categories Propose different levels 5 Improve lab practice and organization 4 More result discussion and stat analysis of it 3 Suggestions on detail content points 3 More lab 1 Other suggestions 2

13 GMO Testing Glossary Thank you Charlotte Leonhardt For calling for entries and collecting terms Mailing lists: 1 st Global GMO meeting participants ISTA Dear colleague, The ISTA GMO Task Force was established in 2001 to facilitate and harmonize the testing for adventitious presence of genetically modified seed. One permanent task of the group is information exchange through ISTA s webpage and workshops. As part of this effort, we are currently in the process of preparing a glossary of GMO related terms for the online information platform ( The glossary should contain definitions of key terminology and commonly used acronyms and should facilitate a common understanding. Would you please provide us with a list of GMO related terms that you consider to be of significance and would be interested to see in this glossary. Your contribution is important to obtain a comprehensive list. We thank you in advance for taking the time to respond. Please send your replies to Charlotte Leonhardt [Charlotte.leonhardt@ages.at] preferably before 28 Feb, Cordially Yours,

14 ~350 Terms TERMS A accuracy Adventitious presence Adventitious seed Closeness of agreement between a test result and the accepted reference value SOURCE Sotiris Kosmas (GR); Sofia Tahar Pina (CHL) Pina (CHL) Agrobacterium tumefaciens Agronomic traits Alleles Amino acid Soil bacterium naturally able to transfer parts of its genetic material to plant cells. It has thus been used as a tool for genetically engineering plants Alleles are the different variant forms of a gene at a particular position (locus) on a chromosome Building blocks of proteins, Amino acids can bind to one another in chains in a virtually infinite number of combinations to form large protein molecules Silmar (BR); GMO compass; diolog gentechnik, Pina (CHL) Pina (CHL) Silmar (BR) Silmar (BR), Pina (CHL) Wild-type Y YAC ( Yeast artificial chromosome ) Z Zygote ΔCΤ method ΔΔCT method The type or form of an organism or gene that occurs most frequently in nature. Often refers to how organisms or genes are found naturally, that is, in the wild, before researchers induced mutations Haldemann (PCRlinks.com) Constructed from yeast DNA, it is a vector used to clone large DNA fragments The diploid cell formed by the fusion of two haploid gametes during fertilization in eukaryotic organisms with sexual reproduction Haldemann (PCRlinks.com); Silmar (BR) Silmar (BR) Sotiris Kosmas (GR) Sotiris Kosmas (GR)

15 Thank You! ISTA GMO Task Force - Info Exchange WG