Research & Development in Hatchery Technology for Asian Seabass & other marine species

Transcription

1 Offshore Mariculture Conference May 2018, Singapore Research & Development in Hatchery Technology for Asian Seabass & other marine species Mr Lim Huan Sein Director, Aquaculture Technology Department, Agri-Food & Veterinary Authority of Singapore (AVA) Spearheading Marine Aquaculture in the Tropics 1

2 Content 1. Overview of Singapore Aquaculture 2. AVA s Marine Aquaculture Centre 3. R&D in Hatchery Technology 4. Genetic Improvement of Asian seabass 5. Technology Transfer to Industry 6. Moving Forward 2

3 Overview of SG Aquaculture Local Production of Food Fish: (~4,800 tonnes) 10% 3

4 Overview of SG Aquaculture Coastal fish farming Open net cage farming Land-based farming Floating closed containment system Singapore Aquaculture Technologies Open sea farming Pond-based farms Vertical RAS farm Apollo Aquaculture Group Barramundi Asia 4

5 Overview of SG Aquaculture AVA s Marine Aquaculture Centre set up to spearhead tropical aquaculture technology development for large scale hatcheries 5

6 AVA s Marine Aquaculture Centre Role of MAC Spearhead tropical aquaculture technology development Key R&D initiatives include: Selective Breeding Large-scale fry production technology Development of new species Aquaculture systems Research institutes & companies welcomed to conduct aquaculture research and commercial trials at MAC. Experimental tanks Broodstock tanks Tank systems at MAC Our Value Proposition: Good source of seawater Purpose-build aquaculture research facility Ease of access to biological materials (e.g. microalgae, rotifers, artemia, eggs/ larvae/ fingerlings of various marine species and shrimp) Bio-secured Availability of lab facilities Availability of teaching facilities (e.g. auditorium) Team of competent & experienced technical staff Visit us on Thursday! 6

7 R&D in Hatchery Technology at MAC Transformation of hatchery production Key components: Live feeds production Lack of consistent supply of quality fry is bottleneck to intensive aquaculture SG s constraints meant that prevalent outdoor pond system is not possible need to develop intensive indoor technology Technologies: Live Feed, Vaccination, Weaning, System design Led to 10X improvement in seabass fry survival and 100X intensification over outdoor pond system Culture protocols System design 7

8 R&D in Hatchery Technology at MAC Development of large-scale fry production technology (Asian seabass) 1. Optimisation of feeding protocol Shortening weaning period from 3 weeks to 1 week Reduced cannibalism Increase survival rate to 50% 2. Water quality management (flow-through) During weaning, water quality is maintained through: increasing water flow, reducing density use of purpose-built weaning tanks (for ease of operation) 8

9 R&D in Hatchery Technology at MAC Challenges of flow-through hatchery production Feeding regime for seabass larviculture Opportunistic (r-selective) bacteria like Vibrio spp. can overwhelm the culture system very quickly when there are excess nutrients in water! Removal of fish wastes & uneaten feeds from the culture tank: Toxic ammonia (NH 3 /NH 4 ) - <0.5ppm TAN Suspended solids (SS) linked to gill cover deformity High bacteria load disease outbreak 9

10 R&D in Hatchery Technology at MAC Disease outbreak in flow-through Big Belly Disease With intensification, hatchery productions of Asian seabass were severely affected by a novel disease called Big-Belly disease. Pathogen is an intracellular bacteria and very similar to Vibrio spp. Big Belly Disease* Size of fish affected: Fry (18-30 days) to 20g Clinical signs: Darkened body and swollen abdomen Very thin tail Aggregation of internal organs Severity: Up to 80% cumulative mortality within 1 week * Disease first described by Intervet/MSD 10

11 R&D in Hatchery Technology at MAC Overcoming BB with low salinity Survival rates of BB-affected fry: 30ppt flow-through vs 10ppt RAS Big-belly disease can be controlled by reducing the salinity from 30ppt to 10ppt Result: Cumulative mortality for 30ppt-FTS group reached 90% but the group in 10ppt- RAS remained below 10% 11

12 R&D in Hatchery Technology at MAC Growth of seabass fry is not affected by low salinity Effect of salinity on hatching rate & buoyancy of larvae Buoyancy test of newly hatched larvae 28-30ppt is required for Day

13 R&D in Hatchery Technology at MAC Results: Improved seabass larviculture protocol using low salinity RAS Salinity of >28ppt is necessary for early larval rearing 10ppt by DAH 30 Salinity adjustment to control Big Belly for seabass fry production If there is any clinical sign of BB, lower the salinity 5ppt to suppress disease manifestation 13

14 R&D in Hatchery Technology at MAC Microbial control strategy: Competitive Exclusion by K-selective bacteria Design of a Compact RAS for Hatchery Production Source: Kari Attramadal et al. RAS system: Matured bacterial content in water less likely to be perturbed by bacteria from inputs such as rotifer and feed Compact RAS with a footprint of 1m 2 can support a production of 120K seabass fry (total culture area of 9m 2 ) 14

15 R&D in Hatchery Technology at MAC Development of hatchery technology for other farmed species Overcoming shock syndrome and improving body coloration for Crimson Red Snapper Optimizing of culture and feeding protocols for Pompano and Cobia Pompano larvae Cobia larvae Pompano fingerlings Cobia Fingerlings 15

16 R&D in Hatchery Technology at MAC Continuous Improvement in live feeds production (rotifer) Outdoor pond culture Indoor batch culture RAS continuous culture Culture volume m m m 3 Density ind/ml ind/ml ind/ml Productivity (rotifer produced per m 3 per day) Man-hour to produce 1billion rotifer 10 mil 100 mil 200 mil 3.9 hr 8.7 hr 3.8 hr Transition from indoor batch culture method to RAS continuous culture method Improved productivity and reduced manpower needs 16

17 Genetics Improvement of Asian seabass Optimising inputs for local farms Developing superior broodstocks through selective breeding Partnered Temasek Life Sciences laboratory (TLL) to leverage on advanced molecular biotechnology to help select better fish Higher productivity for farms using superior seabass 17

18 Technology Transfer to Industry Local hatcheries Overseas Hatchery Coastal Fish Farms Setup of RAS-based hatchery to overcome disease issues Technical Consultancy to setup of marine fish hatchery in Indonesia Demonstration of productivity gains through the adoption of automatic fish grading system Assistance in rotifer culture at floating hatchery Technical assistance & supply of Seabass Fry in expansion of local land-based nursery Fish Supply Back to SG Demonstration of deployment of closed containment system 18

19 Moving Forward Marine hatchery production has progressed from pond-based to indoor RAS technology Further improvements in seeds, nutrition, health and systems can be expected in future Productivity Superior Inputs Genetically Improved Seeds Indoor RAS Animal nutrition & health solutions Indoor Tank Flow-thru Smart Farming Systems Pond-based Flow-thru Level of technology sophistication 19

20 THANK YOU 20