MARTIN COUNTY BOARD OF COUNTY COMMISSIONERS

Transcription

1 MARTIN COUNTY BOARD OF COUNTY COMMISSIONERS DOUG SMITH Commissioner, District 1 UTILITIES & SOLID WASTE DEPARTMENT PO Box 9000 Stuart, FL John E. Polley Director Phone (772) Fax (772) ED FIELDING Commissioner, District 2 HAROLD E. JENKINS II Commissioner, District 3 SARAH HEARD Commissioner, District 4 EDWARD V. CIAMPI Commissioner, District 5 TARYN KRYZDA, CPM County Administrator SARAH W. WOODS Acting County Attorney May 2, 2017 Department of Environmental Protection Domestic Wastewater Program DEP DW Shared Mailbox sed.wastewater@dep.state.fl.us Re: Martin County Utilities North WWTF, Facility # FLA Somatic coliphage monitoring report Dear DEP: Attached please see the report and supplemental documents on somatic coliphage monitoring of the Neutralizer biosolids treatment process prepared by BCR Environmental. Submittal of this report is a condition of permit FLA DW1P, Section VI, Schedules, #2. Coliphage monitoring was performed by BCR for one year subsequent to placing the new Neutralizer process into operation at the facility. If you have any questions, please contact William Thiel, Laboratory Manager, at (772) or by wthiel@martin.fl.us. Sincerely, Todd Leyland Treatment Plant Operations Administrator TL/wt/cb Enclosures C: Jeff Christian, Environmental Specialist, DEP, jeff.christian@dep.state.fl.us William Thiel, Laboratory Manager, wthiel@martin.fl.us Robert Plummer, Treatment Plant Operations Manager, rplummer@martin.fl.us Vena Noble, Chief Plant Operator, vnoble@martin.fl.us TELEPHONE WEB ADDRESS 1 of 39 usd2017l400.docx

2 MARTIN COUNTY BOARD OF COUNTY COMMISSIONERS DOUG SMITH Commissioner, District 1 UTILITIES & SOLID WASTE DEPARTMENT PO Box 9000 Stuart, FL John E. Polley Director Phone (772) Fax (772) ED FIELDING Commissioner, District 2 HAROLD E. JENKINS II Commissioner, District 3 SARAH HEARD Commissioner, District 4 EDWARD V. CIAMPI Commissioner, District 5 TARYN KRYZDA, CPM County Administrator SARAH W. WOODS Acting County Attorney May 2, 2017 Department of Environmental Protection Domestic Wastewater Program DEP DW Shared Mailbox sed.wastewater@dep.state.fl.us Re: Martin County Utilities North WWTF, Facility # FLA Somatic coliphage monitoring report Dear DEP: Attached please see the report and supplemental documents on somatic coliphage monitoring of the Neutralizer biosolids treatment process prepared by BCR Environmental. Submittal of this report is a condition of permit FLA DW1P, Section VI, Schedules, #2. Coliphage monitoring was performed by BCR for one year subsequent to placing the new Neutralizer process into operation at the facility. If you have any questions, please contact William Thiel, Laboratory Manager, at (772) or by wthiel@martin.fl.us. Sincerely, Todd Leyland Treatment Plant Operations Administrator TL/wt/cb Enclosures C: Jeff Christian, Environmental Specialist, DEP, jeff.christian@dep.state.fl.us William Thiel, Laboratory Manager, wthiel@martin.fl.us Robert Plummer, Treatment Plant Operations Manager, rplummer@martin.fl.us Vena Noble, Chief Plant Operator, vnoble@martin.fl.us TELEPHONE WEB ADDRESS 2 of 39 usd2017l400.docx

3 4/27/2017 Laura Boczek EPA 26 West Martin Luther King Drive Cincinnati OH Dear Laura, On November 11 th 2010, BCR Environmental submitted an equivalent PFRP process proposal and was approved by the PEC as a process that was equivalent to a PFRP process in accordance with 40 CFR (a)(8). In order for the Neutralizer to be operating as an equivalent PFRP process, the following conditions must be met: The Neutralizer process is to be used to treat waste activated sludge with a total solids concentration less than or equal to 4% Process Operations must be at a temperature greater than or equal to 15 C (59 F) Following addition of stage 1 Oxidant (chlorine dioxide) ORP is required to be maintained at a value greater than or equal to +100 mv ORP with a contact time at +100 mv ORP 1 hour Stage 1 treatment is followed by Stage 2 treatment which is defined as Nitrite dose = 1500 mg/l sodium nitrite (NaNO 2) with ph during NaNO 2 contact time to 2.3s.u. ORP during stage 2 must be > +100 mv and nitrite contact time at specified ph and ORP is at least 6 hours A limitation on the evaluation of the process was claimed by the EPA because the three facilities that conducted Neutralizer testing were done within the same county (Clay). Therefore, new installations of a Neutralizer process must demonstrate pathogen reduction by verifying somatic coliphage reduction when operating as above. Sampling and analysis of somatic phage must be conducted for a period of at least one year in accordance to the sampling method described at 40 CFR (a). This approval letter is attached for your reference. BCR performed this testing over a period of 12 months at North Jenson WWTP (3100 NW Hillman Drive, Jensen Beach, FL 34957) and all analyses resulted in non-detect or greater than a 3-log reduction (greater than 99.9% reduction) for somatic phage using ASTM 4994, the test results are summarized below: 3 of 39

4 North Jensen Sample Date Raw Treated 10/21/ /9/ /6/ /12/ /11/ BCR also performed five (5) somatic phage tests at Tropical Farms WWTP (8595 SW Kansas Avenue, Stuart, FL 34997) over a period of 12 months in The table below summarizes these results: Sample Date Tropical Farms Raw (PFU/dry gram) Treated (PFU/dry gram) 10/21/ /5/ /6/ /12/ /11/ All samples were taken in accordance with sampling procedures detailed in the Neutralizer Process QAPP, which was previously approved by the PEC. All lab reports are also attached for your review. As BCR Environmental has met the requirements of this monitoring condition as specified in the National Equivalency approval letter dated Nov 16, 2010, we are requesting removal of the somatic phage sampling and analysis provision for the Neutralizer process at these facilities. Sincerely, Fred P Mussari 4 of 39

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8 Biological Consulting Services of North Florida, Inc. October 26, 2015 Dr. Fred P. Mussari BCR Environmental 3740 St. Johns Bluff Rd. S. Ste 21 Jacksonville FL Client ID: TF-Raw, TF-Treated, NJ-Raw, NJ-Treated BCS ID: , , , Dear Dr. Fred P. Mussari, We have completed the analysis of the submitted samples as outlined below. Project Name: MCUA Tropical Farms Analysis Method Fecal Coliforms MPN EPA 1681; SM 9221E; BCS SOP B-4 Coliphage BCS SOP V-11 Following, you will find our report on the results of the analysis coducted on the referenced samples. Should you have any questions, please do not hesitate to contact me. Sincerely, George Lukasik, Ph.D. Laboratory Director Page 1 of 6 Final Report BCS ID , , , of 39

9 Client: BCR Environmental Analysis: Coliphage Project Name: MCUA Tropical Farms Client Sample ID: TF-Raw BCS Sample ID: Sample Description: Biosolids liquid Sampling Date: October 21, :16 Percent Solids: 4.6 Date Received: October 23, :37 Receipt Temperature: 7.9 deg C Ice: No Amount Sampled: 50 g Analyst: David Sekora Analysis Start: October 23, :35 Analysis Stop Date: October 24, :00 Primary Value: 3,632 PFU/mL sample analyzed Secondary Value: 1,580 PFU /dry gram Qualifier: Analysis Notes: None Client: Analysis: Project Name: BCR Environmental Fecal Coliforms MPN MCUA Tropical Farms Client Sample ID: TF-Treated BCS Sample ID: Sampling Date: October 22, :55 Amount Sampled: 198 Analysis Start: October 23, :23 Primary Value: 0.02 Secondary Value: 0.4 Qualifier: Analysis Notes: ml Analysis Stop Date: October 24, :30 Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Description: Biosolids liquid Percent Solids: 4.3 Date Received: October 23, :37 Receipt Temperature: 7.9 deg C Analyst: Monique Bessette Ice: No U Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 2 of 6 Final Report BCS ID , , , of 39

10 Client: Analysis: Project Name: BCR Environmental Coliphage MCUA Tropical Farms Client Sample ID: TF-Treated BCS Sample ID: Sampling Date: October 22, :55 Amount Sampled: 50 Analysis Start: October 23, :35 Primary Value: 2.00 Secondary Value: 0.9 Qualifier: Analysis Notes: g PFU/mL sample analyzed PFU /dry gram Sample Description: Biosolids liquid Percent Solids: 4.3 Date Received: October 23, :37 Receipt Temperature: 7.9 deg C Analyst: David Sekora Ice: No Analysis Stop Date: October 24, :00 U None; Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Client: BCR Environmental Analysis: Coliphage Project Name: MCUA North Jensen Client Sample ID: NJ-Raw BCS Sample ID: Sample Description: Biosolids liquid Sampling Date: October 21, :50 Percent Solids: 3.5 Date Received: October 23, :00 Receipt Temperature: 7.9 deg C Ice: No Amount Sampled: 50 g Analyst: David Sekora Analysis Start: October 23, :35 Analysis Stop Date: October 24, :00 Primary Value: 14,688 PFU/mL sample analyzed Secondary Value: 8,440 PFU /dry gram Qualifier: Analysis Notes: None Page 3 of 6 Final Report BCS ID , , , of 39

11 Client: Analysis: Project Name: BCR Environmental Fecal Coliforms MPN MCUA North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: October 22, :25 Amount Sampled: 183 Analysis Start: October 23, :20 Primary Value: 0.02 Secondary Value: 0.4 Qualifier: Analysis Notes: ml Analysis Stop Date: October 24, :30 Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Description: Biosolids liquid Percent Solids: 4.6 Date Received: October 23, :00 Receipt Temperature: 7.9 deg C Analyst: Monique Bessette Ice: No U Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Client: Analysis: Project Name: BCR Environmental Coliphage MCUA North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: October 22, :25 Amount Sampled: 50 Analysis Start: October 23, :35 Primary Value: 2.00 Secondary Value: 0.9 Qualifier: Analysis Notes: g PFU/mL sample analyzed PFU /dry gram Sample Description: Biosolids liquid Percent Solids: 4.6 Date Received: October 23, :00 Receipt Temperature: 7.9 deg C Analyst: David Sekora Ice: No Analysis Stop Date: October 24, :00 U None; Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 4 of 6 Final Report BCS ID , , , of 39

12 Client: Project: BCR Environmental MCUA Tropical Farms Method Detection Limit: 1 microorganism/ unit weight or volume analyzed Practical Quantitation Limit: 1 microorganism/ unit weight or volume analyzed Report Notes: None to report I certify that I have personally examined and am familiar with the information submitted herein. Based on my inquiry of those individuals immediately responsible for obtaining the information, I believe the submitted information is true, accurate, and complete. The above results relate only the collected and referenced sample(s) and to their condition at the time of process. The analysis results are not indicative or representative of a process. Sampling and field data information was submitted by the client. The analysis and presented results in this report meet the requirements of The NELAC Institute (TNI), ISO 17025, and The State of Florida Department of Public Health s Laboratory Certification Program, as applicable unless otherwise noted. Signature of Laboratory Director/Authorized Rep. Date: October 26, 2015 Page 5 of 6 Final Report BCS ID , , , of 39

13 DATA QUALIFIER CODES SYMBOL MEANING B Results based on counts outside the acceptable range. D Measurement was made in the field. The reported value is between the laboratory method detection limit and the laboratory practical I quantitation limit. J1 The sample matrix interfered with the ability to make any accurate determination. J2 No Quality Control criteria exist for the component. J3 The data are questionable because of improper laboratory or field protocols. L Off scale high. Actual value is known to be greater than value given. Presumptive evidence for the presence of material. There is an indication that the analyte is presen N but the confirmation requirement was not met. O Sampled, but analysis not performed. Q Sample held beyond the accepted holding time. Indicates that the compound was analyzed for but not detected. The specified component was not U detected. The reported value is the method detection limit. V Analyte was detected in both sample and associated method blank. Data may not be accurate. Y The laboratory analysis was from an improperly preserved sample. The data may not be accurate. Too many colonies present (TNTC); the numeric value given represents the upper end of the value t Z can be determined based on the volume. Data are rejected and should not be used. QC data for analyte did not meet acceptance criteria.? Presence or absence of analyte could not be confirmed. ** Not reported due to interference.! Data deviate from historically established concentration range. # BCS Lab specific qualifier. See laboratory analysis notes. Page 6 of 6 Final Report BCS ID , , , of 39

14 Biological Consulting Services of North Florida, Inc. February 12, 2016 Dr. Fred P. Mussari BCR Environmental 3740 St. Johns Bluff Rd. S. Ste 21 Jacksonville FL Client ID: NJ-Raw, NJ-Treated BCS ID: , Dear Dr. Fred P. Mussari, We have completed the analysis of the submitted samples as outlined below. Project Name: North Jensen Analysis Method Fecal Coliforms MPN EPA 1681; SM 9221E; BCS SOP B-4 Coliphage BCS SOP V-11 Following, you will find our report on the results of the analysis conducted on the referenced samples. Should you have any questions, please do not hesitate to contact me. Sincerely, George Lukasik, Ph.D. Laboratory Director Page 1 of 5 Final Report BCS ID , of 39

15 Client: BCR Environmental Analysis: Coliphage Project Name: North Jensen Client Sample ID: NJ-Raw BCS Sample ID: Sample Description: Biosolids liquid Sampling Date: February 09, :30 Percent Solids: 5.3 Date Received: February 11, :00 Receipt Temperature: 4.3 deg C Ice: Yes Amount Sampled: 50.7 g Analyst: David Sekora, M.S. Analysis Start: February 11, :06 Analysis Stop Date: February 12, :30 Primary Value: 15,341 PFU/mL sample analyzed Secondary Value: 5,710 PFU /dry gram Qualifier: Analysis Notes: None Client: Analysis: Project Name: BCR Environmental Fecal Coliforms MPN North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: February 10, :00 Amount Sampled: 98.8 ml Analysis Start: February 11, :31 Primary Value: 0.18 Secondary Value: 4.9 Qualifier: Analysis Notes: Analysis Stop Date: February 12, :38 Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Description: Biosolids liquid Percent Solids: 3.7 Date Received: February 11, :00 Receipt Temperature: 4.3 deg C Analyst: Jennifer Price Ice: Yes U None; Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 2 of 5 Final Report BCS ID , of 39

16 Client: Analysis: Project Name: BCR Environmental Coliphage North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: February 10, :00 Amount Sampled: g Analysis Start: February 11, :06 Primary Value: 3.5 Secondary Value: 2.0 Qualifier: Analysis Notes: PFU/mL sample analyzed PFU /dry gram Sample Description: Biosolids liquid Percent Solids: 3.7 Date Received: February 11, :00 Receipt Temperature: 4.3 deg C Analyst: David Sekora, M.S. Ice: Yes Analysis Stop Date: February 12, :30 U None; Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 3 of 5 Final Report BCS ID , of 39

17 Client: Project: BCR Environmental North Jensen Method Detection Limit: 1 microorganism/ unit weight or volume analyzed Practical Quantitation Limit: 1 microorganism/ unit weight or volume analyzed Report Notes: None to report I certify that I have personally examined and am familiar with the information submitted herein. The results presented pertain only to the samples analyzed associated identifier number(s). Based on my inquiry of the individuals immediately responsible for obtaining the information, I believe the submitted data are true, accurate, and complete. Sampling field data information was obtained from the submitted sampling documents. The analysis was authorized commissioned by the client. The data provided is strictly representative of the analysis conducted on the material/samples/articles provided by the client (or client s representative) it s (their) condition at the time of test. The sample(s) were analyzed in accordance with the method described for each analyte, however due to the inherent limitation(s) of method(s), microorganisms may avoid detection. Thus, BCS Laboratories offers no express or implied warranties concerning the quality, safety, and/or purity of any sample, batch, source, or the process they are derived from. Positive, negative, and blank controls were performed as outlined in the method and as per Good Laboratory Practices. The analysis results presented in this report meet the requirements of The NELAC Institute (TNI), ISO 17025, The State of Florida Department of Public Health s Laboratory Certification Program, as applicable unless otherwise noted. Signature of Laboratory Director/Authorized Rep. Date: February 12, 2016 Page 4 of 5 Final Report BCS ID , of 39

18 DATA QUALIFIER CODES SYMBOL MEANING B Results based on counts outside the acceptable range. D Measurement was made in the field. The reported value is between the laboratory method detection limit and the laboratory practical I quantitation limit. J1 The sample matrix interfered with the ability to make any accurate determination. J2 No Quality Control criteria exist for the component. J3 The data are questionable because of improper laboratory or field protocols. L Off scale high. Actual value is known to be greater than value given. Presumptive evidence for the presence of material. There is an indication that the analyte is N present, but the confirmation requirement was not met. O Sampled, but analysis not performed. Q Sample held beyond the accepted holding time. Indicates that the compound was analyzed for but not detected. The specified component was U not detected. The reported value is the method detection limit. V Analyte was detected in both sample and associated method blank. Data may not be accurate. Y The laboratory analysis was from an improperly preserved sample. The data may not be Too many colonies present (TNTC); the numeric value given represents the upper end of the Z value that can be determined based on the volume. Data are rejected and should not be used. QC data for analyte did not meet acceptance criteria.? Presence or absence of analyte could not be confirmed. ** Not reported due to interference.! Data deviate from historically established concentration range. # BCS Lab specific qualifier. See laboratory analysis notes. Page 5 of 5 Final Report BCS ID , of 39

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20 Biological Consulting Services of North Florida, Inc. April 11, 2016 Casey Cochran Nuterra Management 3740 St. Johns Bluff Rd South Suite 21 Jacksonville FL Client ID: NJ-Raw, NJ-Treated BCS ID: , Dear Casey Cochran, We have completed the analysis of the submitted samples as outlined below. Project Name: North Jensen Analysis Method Fecal Coliforms MPN EPA 1681; SM 9221E; BCS SOP B-4 (ISO17025 and TNI Accredited) Coliphage BCS SOP V-11 (ISO17025 and TNI Accredited) Following, you will find our report on the results of the analysis conducted on the referenced samples. Should you have any questions, please do not hesitate to contact me. Sincerely, George Lukasik, Ph.D. Laboratory Director Page 1 of 5 Final Report BCS ID , of 39

21 Client: Nuterra Management Analysis: Coliphage Project Name: North Jensen Client Sample ID: NJ-Raw BCS Sample ID: Sample Description: Biosolids Sampling Date: April 06, :00 Percent Solids: 4.2 Date Received: April 08, :25 Receipt Temperature: 5.2 deg C Ice: Yes Amount Sampled: g Analyst: David Sekora, M.S. Analysis Start: April 08, :00 Analysis Stop Date: April 09, :45 Primary Value: 4,109 PFU/mL sample analyzed Secondary Value: 3,870 PFU /dry gram Qualifier: Analysis Notes: None Client: Analysis: Project Name: Nuterra Management Fecal Coliforms MPN North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: April 07, :00 Amount Sampled: Analysis Start: April 08, :48 Primary Value: 1.8 Secondary Value: 46.2 Qualifier: Analysis Notes: ml Analysis Stop Date: April 09, :03 Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Description: Biosolids Percent Solids: 3.9 Date Received: April 08, :25 Receipt Temperature: 5.2 deg C Analyst: Jennifer Price Ice: Yes U Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 2 of 5 Final Report BCS ID , of 39

22 Client: Analysis: Project Name: Nuterra Management Coliphage North Jensen Client Sample ID: NJ-Treated BCS Sample ID: Sampling Date: April 07, :00 Amount Sampled: Analysis Start: April 08, :00 Primary Value: 7.0 Secondary Value: 3.7 Qualifier: Analysis Notes: g PFU/mL sample analyzed PFU /dry gram Sample Description: Biosolids Percent Solids: 3.7 Date Received: April 08, :25 Receipt Temperature: 5.2 deg C Analyst: David Sekora, M.S. Ice: Yes Analysis Stop Date: April 09, :45 U None; Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 3 of 5 Final Report BCS ID , of 39

23 Client: Project: Nuterra Management North Jensen Method Detection Limit: 1 microorganism/ unit weight or volume analyzed Practical Quantitation Limit: 1 microorganism/ unit weight or volume analyzed Report Notes: None to report I certify that I have personally examined and am familiar with the information submitted herein. The results presented pertain only to the samples analyzed associated identifier number(s). Based on my inquiry of the individuals immediately responsible for obtaining the information, I believe the submitted data are true, accurate, and complete. Sampling field data information was obtained from the submitted sampling documents. The analysis was authorized commissioned by the client. The data provided is strictly representative of the analysis conducted on the material/samples/articles provided by the client (or client s representative) it s (their) condition at the time of test. The sample(s) were analyzed in accordance with the method described for each analyte, however due to the inherent limitation(s) of method(s), microorganisms may avoid detection. Thus, BCS Laboratories offers no express or implied warranties concerning the quality, safety, and/or purity of any sample, batch, source, or the process they are derived from. Positive, negative, and blank controls were performed as outlined in the method and as per Good Laboratory Practices. The analysis results presented in this report meet the requirements of The NELAC Institute (TNI), ISO 17025, The State of Florida Department of Public Health s Laboratory Certification Program, as applicable unless otherwise noted. Signature of Laboratory Director/Authorized Rep. Date: April 11, 2016 Page 4 of 5 Final Report BCS ID , of 39

24 DATA QUALIFIER CODES SYMBOL MEANING B Results based on counts outside the acceptable range. D Measurement was made in the field. The reported value is between the laboratory method detection limit and the laboratory I practical quantitation limit. J1 The sample matrix interfered with the ability to make any accurate determination. J2 No Quality Control criteria exist for the component. J3 The data are questionable because of improper laboratory or field protocols. L Off scale high. Actual value is known to be greater than value given. Presumptive evidence for the presence of material. There is an indication that the analyte is N present, but the confirmation requirement was not met. O Sampled, but analysis not performed. Q Sample held beyond the accepted holding time. Indicates that the compound was analyzed for but not detected. The specified component was U not detected. The reported value is the method detection limit. V Analyte was detected in both sample and associated method blank. Data may not be accurate. Y The laboratory analysis was from an improperly preserved sample. Data may not be accurate. Too many colonies present (TNTC); the numeric value given represents the upper end of the Z value that can be determined based on the volume. Data are rejected and should not be used. QC data for analyte did not meet acceptance criteria.? Presence or absence of analyte could not be confirmed. ** Not reported due to interference.! Data deviate from historically established concentration range. # BCS Lab specific qualifier. See laboratory analysis notes. ^ BCS is not currently accredited for this analyte. Page 5 of 5 Final Report BCS ID , of 39

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26 Biological Consulting Services of North Florida, Inc. July 15, 2016 Casey Cochran Nuterra Management 3740 St. Johns Bluff Rd South Suite 21 Jacksonville FL Client ID: North Jensen/Raw, North Jensen/Treated BCS ID: , Dear Casey Cochran, We have completed the analysis of the submitted samples as outlined below. Project Name: North Jensen Analysis Method Fecal Coliforms MPN EPA 1681; SM 9221E; BCS SOP B-4 (ISO17025 and TNI Accredited) Coliphage (male specific & somatic) Total Coliphage BCS SOP V-11 (ISO17025 and TNI Accredited) Following, you will find our report on the results of the analysis conducted on the referenced samples. Should you have any questions, please do not hesitate to contact me. Sincerely, Bonnie Mull, MPH Laboratory Operations Manager Page 1 of 6 Final Report BCS ID , of 39

27 Client: Nuterra Management Analysis: Coliphage (male specific & somatic) Project Name: North Jensen Client Sample ID: North Jensen/Raw Sample Date/Time: July 12, :00 BCS Sample ID: Sample Description: Biosolids Date Received: July 14, :50 Percent Solids: 3.9 Volume Analyzed: g Receipt Temperature: 2.1 deg C Ice: Yes Analysis Start: July 14, :11 Analysis Stop Date: July 15, :30 Analyst: David Sekora, M.S. Qualifier: None Primary Value: 1,731 PFU/mL sample analyzed Secondary Value: 893 PFU /dry gram Analysis Notes: None Client: Analysis: Project Name: Nuterra Management Fecal Coliforms MPN North Jensen Client Sample ID: North Jensen/Treated BCS Sample ID: Date Received: July 14, :50 Volume Analyzed: ml Analysis Start: July 14, :14 Analyst: Monique Bessette, MPH Primary Value: 0.02 Secondary Value: 0.5 Analysis Stop Date: July 15, :50 Qualifier: U Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Date/Time: July 13, :00 Sample Description: Biosolids Percent Solids: 3.3 Receipt Temperature: 2.1 deg C Ice: Yes Analysis Notes: Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 2 of 6 Final Report BCS ID , of 39

28 Client: Analysis: Project Name: Nuterra Management North Jensen Volume Analyzed: Analysis Start: July 14, :11 Primary Value: 16.9 Secondary Value: 10.1 Coliphage (male specific & somatic) Client Sample ID: North Jensen/Treated BCS Sample ID: Date Received: July 14, :50 Analyst: g David Sekora, M.S. PFU/mL sample analyzed PFU /dry gram Sample Date/Time: July 13, :00 Sample Description: Biosolids Percent Solids: 3.3 Receipt Temperature: Analysis Stop Date: July 15, :30 Qualifier: U 2.1 deg C Ice: Yes Analysis Notes: Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units Page 3 of 6 Final Report BCS ID , of 39

29 Client: Project: Nuterra Management North Jensen Method Detection Limit: 1 microorganism/ unit weight or volume analyzed Practical Quantitation Limit: 1 microorganism/ unit weight or volume analyzed Report Notes: Sample(s) were received well preserved and in excellent condition. Sample(s) were analyzed following receipt as per the described analytical methodology. End of report notes. Page 4 of 6 Final Report BCS ID , of 39

30 I certify that I have examined I am familiar with the information submitted herein. The results pertain only to analyzed sample(s) associated identifier #(s). Based on my inquiry of the individuals responsible for the analysis, I believe the data to be true, accurate, complete. Sampling field data was obtained from submitted documents. The analysis was authorized commissioned by the client. The resulting data are representative of the analysis conducted on the material/samples/articles provided by the client (or client s representative) it s/their condition at the time of analysis. The sample(s) were analyzed in accordance with the appropriate method, however due to the inherent limitations of the methods, microorganisms may avoid detection. BCS Laboratories offers no express or implied warranties concerning the quality, safety, and/or purity of any sample, batch, source, or the process they are derived from. Quality assurance controls were performed as outlined in the method and as per Good Laboratory Practices. The analysis results presented in this report meet the requirements of The NELAC Institute (TNI), ISO 17025, and The FL Public Health s Laboratory Certification Program, as applicable unless otherwise noted. Signature of Laboratory Director/Authorized Rep. Date: July 15, 2016 Page 5 of 6 Final Report BCS ID , of 39

31 DATA QUALIFIER CODES SYMBOL MEANING B Results based on counts outside the acceptable range. D Measurement was made in the field. The reported value is between the laboratory method detection limit and the laboratory practical I quantitation limit. J1 The sample matrix interfered with the ability to make any accurate determination. J2 No Quality Control criteria exist for the component. J3 The data are questionable because of improper laboratory or field protocols. L Off scale high. Actual value is known to be greater than value given. Presumptive evidence for the presence of material. There is an indication that the analyte is present, N but the confirmation requirement was not met. O Sampled, but analysis not performed. Q Sample held beyond the accepted holding time. Indicates that the compound was analyzed for but not detected. The specified component was not U detected. The reported value is the method detection limit. V Analyte was detected in both sample and associated method blank. Data may not be accurate. Y The laboratory analysis was from an improperly preserved sample. The data may not be accurate. Too many colonies present (TNTC); the numeric value given represents the upper end of the value Z that can be determined based on the volume. Data are rejected and should not be used. QC data for analyte did not meet acceptance criteria.? Presence or absence of analyte could not be confirmed. ** Not reported due to interference. ^ BCS is not currently accredited for this analyte. # BCS Lab specific qualifier. See laboratory analysis notes. Page 6 of 6 Final Report BCS ID , of 39

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33 Biological Consulting Services of North Florida, Inc. October 17, 2016 Casey Cochran Nuterra Management 3740 St. Johns Bluff Rd South Suite 21 Jacksonville FL Client ID: NJ Raw, NJ Treated BCS ID: , Dear Casey Cochran, We have completed the analysis of the submitted samples as outlined below. Project Name: North Jensen Analysis Method Fecal Coliforms MPN EPA 1681; SM 9221E; BCS SOP B-4 (ISO17025 and TNI Accredited) Coliphage (male specific & somatic) Total Coliphage BCS SOP V-11 (ISO17025 and TNI Accredited) Following, you will find our report on the results of the analysis conducted on the referenced samples. Should you have any questions, please do not hesitate to contact me. Sincerely, Bonnie Mull, MPH Laboratory Operations Manager Page 1 of 6 Final Report BCS ID , of 39

34 Client: Nuterra Management Analysis: Coliphage (male specific & somatic) Project Name: North Jensen Client Sample ID: NJ Raw Sample Date/Time: October 11, :30 BCS Sample ID: Sample Description: Biosolids Date Received: October 13, :40 Amount Submitted:125 ml % Solids: 4.0 Amount Analyzed: g Receipt Temperature: 7.5 deg C Preserved: Yes Analysis Start: October 13, :30 Analysis Stop Date: October 15, :45 Analyst: David Sekora, M.S. Qualifier: None Primary Value: 866 PFU/mL sample analyzed Secondary Value: 846 PFU /dry gram Analysis Notes: None Client: Analysis: Project Name: Nuterra Management Fecal Coliforms MPN North Jensen Client Sample ID: NJ Treated BCS Sample ID: Date Received: October 13, :40 Amount Analyzed: Analysis Start: October 13, :15 Analyst: ml Melanie O'Rourke, B.S. Primary Value: 0.18 Secondary Value: 5.0 Analysis Stop Date: October 14, :15 Qualifier: Fecal Coliforms MPN/mL sample analyzed Fecal coliforms/dry gram Sample Date/Time: October 12, :00 Sample Description: Biosolids Amount Submitted:125 ml Receipt Temperature: Analysis Notes: Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units U 7.5 deg C % Solids: 3.6 Preserved: Yes Page 2 of 6 Final Report BCS ID , of 39

35 Client: Analysis: Project Name: Nuterra Management North Jensen Client Sample ID: NJ Treated BCS Sample ID: Amount Analyzed: Analysis Start: October 13, :30 Primary Value: 4.2 Secondary Value: 4.6 Coliphage (male specific & somatic) Date Received: October 13, :40 Analyst: g David Sekora, M.S. PFU/mL sample analyzed PFU /dry gram Sample Date/Time: October 12, :00 Sample Description: Biosolids Amount Submitted:125 ml Receipt Temperature: Analysis Stop Date: October 15, :45 Qualifier: Analysis Notes: Undetected: Analyte was not detected in the sample analyzed; Value represents the method's detection limit for the amount of sample analyzed as per the method's standard reporting units U 7.5 deg C % Solids: 3.6 Preserved: Yes Page 3 of 6 Final Report BCS ID , of 39

36 Client: Project: Nuterra Management North Jensen Method Detection Limit: 1 microorganism/ unit weight or volume analyzed Practical Quantitation Limit: 1 microorganism/ unit weight or volume analyzed Report Notes: Sample(s) were received well preserved and in excellent condition. Sample(s) were analyzed following receipt as per the described analytical methodology. End of report notes. Page 4 of 6 Final Report BCS ID , of 39

37 I certify that I have examined I am familiar with the information submitted herein. The results pertain only to analyzed sample(s) associated identifier #(s). Based on my inquiry of the individuals responsible for the analysis, I believe the data to be true, accurate, complete. Sampling field data was obtained from submitted documents. The analysis was authorized commissioned by the client. The resulting data are representative of the analysis conducted on the material/samples/articles provided by the client (or client s representative) it s/their condition at the time of analysis. The sample(s) were analyzed in accordance with the appropriate method, however due to the inherent limitations of the methods, microorganisms may avoid detection. BCS Laboratories offers no express or implied warranties concerning the quality, safety, and/or purity of any sample, batch, source, or the process they are derived from. Quality assurance controls were performed as outlined in the method and as per Good Laboratory Practices. The analysis results presented in this report meet the requirements of The NELAC Institute (TNI), ISO 17025, and The FL Public Health s Laboratory Certification Program, as applicable unless otherwise noted. Signature of Laboratory Director/Authorized Rep. Date: October 17, 2016 Page 5 of 6 Final Report BCS ID , of 39

38 DATA QUALIFIER CODES SYMBOL MEANING B Results based on counts outside the acceptable range. D Measurement was made in the field. The reported value is between the laboratory method detection limit and the laboratory practical I quantitation limit. J1 The sample matrix interfered with the ability to make any accurate determination. J2 No Quality Control criteria exist for the component. J3 The data are questionable because of improper laboratory or field protocols. L Off scale high. Actual value is known to be greater than value given. Presumptive evidence for the presence of material. There is an indication that the analyte is present, N but the confirmation requirement was not met. O Sampled, but analysis not performed. Q Sample held beyond the accepted holding time. Indicates that the compound was analyzed for but not detected. The specified component was not U detected. The reported value is the method detection limit. V Analyte was detected in both sample and associated method blank. Data may not be accurate. Y The laboratory analysis was from an improperly preserved sample. The data may not be accurate. Too many colonies present (TNTC); the numeric value given represents the upper end of the value Z that can be determined based on the volume. Data are rejected and should not be used. QC data for analyte did not meet acceptance criteria.? Presence or absence of analyte could not be confirmed. ** Not reported due to interference. ^ BCS is not currently accredited for this analyte. # BCS Lab specific qualifier. See laboratory analysis notes. Page 6 of 6 Final Report BCS ID , of 39

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