biomedia Animal Media Microbiological Media Plant Media > accelerate: cellcultureresearch

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2 biomedia 8 Animal Media Microbiological Media Plant Media > accelerate: cellcultureresearch

3 biomedia Animal Animal Cell Culture Media biomedia 122 DMEM Dulbecco s Modified Eagle s Mediuma modification of Basal Medium Eagle (BME). Original formulation of DMEM contained 1000mg/L of glucose, but since has been raised in some modifications to 4500mg/L. Hams F12 nutrient mixture (1:1) was created to determine the media requirements for individual cell types. Hams F12 (1:1) with HEPES Contains: LGlutamine, Pyridoxine Hydrochloride & 15mM HEPES buffer. # # $ 8.15 $ # L powder $ 6.42 # L powder $ # L powder $ Hams F12 (1:1) See above for info Without HEPES # $ 7.91 # $ # L powder $ 5.91 # L powder $ # L powder $ Hams F12 (1:1) See above for info Without Phenol Red # $ 8.13 # $ High Glucose with LGlutamine # $ 8.60 with 25mM HEPES buffer # $ Contains Phenol Red # L powder $ 7.86 # L powder $ # L powder $ High Glucose with LGlutamine (Modification 1) Does not include Sodium Pyruvate, MyoInositol # # $ 8.15 $ High Glucose with LGlutamine (Modification 2) See above for info. Without Phenol Red # # $ # # $ # L powder $ 7.86 # L powder $ # L powder $ High Glucose with LGlutamine (Modification 3) # # $ Does not include # # $ Sodium Pyruvate or # L powder $ 6.49 Sodium Biocarbonate With Pyridoxine Hydrochloride # L powder $ # L powder $ High Glucose with LGlutamine (Modification 4) See above for info Without Sodium Phophate # $ 8.15 # $ High Glucose with LGlutamine (Modification 5) & # # $ mg/L Sodium Pyruvate # # $ With Sodium Pyruvate # L powder $ 6.62 # L powder $ # L powder $

4 High Glucose without LGlutamine (Modification 6) & 25mM HEPES buffer # # $ 8.49 $ High Glucose without LGlutamine (Modification 7) Does not contain Sodium Pyruvate Phenol Red # # $ 8.00 $ High Glucose w/o LGlutamine See above for info With Pyridoxine Hydrochloride # $ 8.04 # $ # L powder $ 6.43 # L powder $ # L powder $ High Glucose without LGlutamine (Modification 1) Hydrochloride & Sodium Pyruvate supplement # # $ 8.10 $ Low Glucose with LGlutamine & 25mM HEPES # # $ 8.61 $ Low Glucose with LGlutamine (Modification 1) & 110mg/L Sodium Pyruvate # # $ 8.15 $ # L powder $ 5.57 # L powder $ # L powder $ Low Glucose without LGlutamine (Modification 2), 25mM HEPES buffer & 110mg/L Sodium Pyruvate # # $ 8.51 $ Low Glucose without LGlutamine without Phenol Red & 110mg/L Sodium Pyruvate # # $ 8.01 $ Low Glucose w/o LGlutamine w/sodium Pyruvate See above for info With Sodium Pyruvate # $ 8.04 # $ No Glucose with LGlutamine Does not contain Sodium Pyruvate or # # # L powder $ 8.04 $ $ 5.81 Sodium Bicarbonate # L powder $ # L powder $ Iscove s with LGlutamine Contains: 25mM HEPES Has been shown to support murine B lymphocytes, # # $ 8.73 $ hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes & a variety of hybrid cells. Iscove s with LGlutamine without Phenol Red Contains: 25mM HEPES Does not contain # $ 8.68 Sodium Bicarbonate or # $ Phenol Red # L powder $ 5.36 # L powder $ # L powder $ biomedia Animal Cell Culture Media 123

5 Fetal Calf Bovine Serum Please inquire for availability & specifications. Heat Inactivated # ml $ F10 with LGlutamine Does not contain Sodium Bicarbonate # $ 7.70 # $ Animal Cell Culture Media biomedia Standard Grade # ml $ Premium Grade # ml $ Grace s Media When supplemented with the proper components, this medium has been shown to support a variety of insect cells, including several lepidopteran as well as some dipterans. With LGlutamine # $ # $ Without LMethionine # $ # $ Supplement (Hink s) # $ # $ # L powder $ # L powder $ F10 with LGlutamine Contains: 25mM HEPES buffer F12 with LAlanine Contains: Dipeptide LAlanine; LGlutamine substituted on a molar equivalent basis for LGlutamine F12 with LGlutamine Does not contain Sodium Bicarbonate F12 with LGlutamine Without Phenol Red # L powder $ 5.91 # L powder $ # $ 8.19 # $ # $ 8.16 # $ # $ 7.81 # $ # L powder $ 5.90 # L powder $ # $ 7.82 Ham s Nutrient Mix Mixtures F10 & F12 were both originally developed to support the growth of a variety of cells, particularly the Chinese Hamster Ovary (CHO) cells, with or without serum supplementation. Ham s F10 has been used in the culture of a wide variety of cells of human & animal origin. Ham s F12 is preferred for the culture of rodent cells & cloning medium for myeloma & hybridoma cells. F12 Kaighn s Modification Contains: LGlutamine > accelerate: cellculture # $ # $ 8.16 # $ for protocols visit

6 Human Serum Human plasma is collected from healthy male donors at FDA licensed facilities located within the U.S. Individual units are converted to serum, pooled, filtered, bottled & cell culture tested for mycoplasma, endotoxin & sterility. Individual units are tested & found negative for all required viral markers via FDA approved methods (HBsAg, HIV1, HIV2, HCV, HIV1Ag or Hiv1 NAT, ALT, & syphilis). Normal # ml $ Heat Inactivated # ml $ At 56 C for 30min IPL41 Insect Medium Designed to support the growth & maintenance of # $ cell lines descending from # $ lepidopteran species & the # L powder $ propagation of viruses within these cells, & for large scale # L powder $ production of baculovirus. Contains: LGlutamine & Calcium Chloride # L powder $ Does not contain Tryptose Phosphate Broth With LGlutamine # $ 8.25 # $ # L powder $ 6.68 # L powder $ # L powder $ Without LGlutamine # $ 8.24 # $ Mccoy s 5a Media # L powder $ 7.75 # L powder $ # L powder $ For use in work with human myeloblasts. Does not contain Sodium Bicarbonate With LGlutamine # $ 8.73 # $ # L powder $ 7.36 # L powder $ # L powder $ biomedia Animal Cell Culture Leibovitz s l15 Medium Formulated to support the growth & proliferation of several cell lines in CO 2 free systems without a sodium bicarbonate supplement. Buffered by its salts, free base amino acids & galactose (substitutes for glucose). Can be used under conditions free of gaseous exchange with the atmosphere. Without LGlutamine # $ 8.75 # $ # L powder $ 7.35 # L powder $ # L powder $ > accelerate: animalcellresearch 125

7 Animal Cell Culture biomedia MCDB 131 Medium Developed for the culture of specific cell types without a serum supplement. # # $ 7.97 $ Supplemented with growth factors, hormones, trace elements or low levels of dialyzed fetal bovine serum protein (FBSP). Medium 199 For growth of explanted tissue. Long term cultivation of cells requires a serum supplement. Used in virology, vaccine production, & the culture of many nontransformed cells. With Earle s Salts and LGlutamine # $ 9.95 # L powder $ 5.83 # L powder $ With Earle s Salts and HEPES Contains: LGlutamine & 25mM HEPES buffer # $ 9.95 # $ With Hank s Salt and LGlutamine # $ 8.16 # $ With Hank s Salt and HEPES Contains: LGlutamine & 25mM HEPES buffer # $ 8.20 # $ Microplate Replicator # well $ biomedia Microbiological Media bioworld formulates a large selection of microbiological media and can custom produce other formulations. For a full line of microbiomedia and media ingredients, please visit & Agar Bacteriological Grade CAS: Melting Point: 8488ºC Gel Point (1.5%): 3438ºC Gel Strength: g/cm 2 Pass through 60 mesh:> 90% LB Broth, Lennox Formulated for the propagation and maintenance of E. coli for molecular biology. Contains: Bacto Tryptone, Yeast Extract, Sodium Chloride ph Medium salt concentration Replica Platters & Valvet Squares To replicate bacterial or yeast colonies on a plate # g $ # g $ # g $ # kg $ # kg $ # mm $ # mm $ # well $ Stainless steal prongs See Valvet & Valvet Squares (page 127) for protocols visit

8 LB Broth, Miller Formulated for the propagation and maintenance of E. coli for molecular biology. Contains: Bacto Trytone, Bacto Yeast Extract, Sodium Chloride ph Higher salt concentration Super Broth An extremely rich medium for obtaining high yields of bacteria and lambdabacteriophage in liquid lysates. Yeast Extract # g $ # kg $ # kg $ # g $ Water soluble portion of # g $ autolyzed yeast and source of # g $ vitamin B complex A high vitamin content provides excellent growth factors for most # kg $ microorganisms. Usually added at a concentration of 35g/L Yeast Nitrogen Base For classification of yeast based on carbon assimilation. Carbon source: Dextrose Nitogen source: Ammonium Sulfate Missing essential amino acid Histidine Histidine Dropout Missing Histidine Leucine Dropout Missing Leucine Tryptophane Dropout Missing Trytophane Uracil Dropout Missing Uracil # L $ # L $ # L $ # L $ UniLawn Inoculation Turntables 2 in 1 100mm, 150mm UniLawn 100mm Velvet Squares 10/pk 6/pk # mm $ # mm $ # x 6 $ # x 9 $ biomedia Microbiology Media Yeast Nitrogen Base without Carbon Source Ammonium sulfate is the sole nitrogen source. Requires the addition of a carbon source. # g $ Velveteen Squares 10/pk 6/pk # x 6 $ # x 9 $ For In vitro lab use only Not for human use or consumption

9 Plant Tissue & Cell Culture Media biomedia biomedia Plant Tissue & Cell Culture Media BioWorld provides a complete line of plant tissue culture media for transformation, regeneration and propagation of plants at very competitive prices. For the full line of plant tissue culture media, please visit Custom formulations are available. MS Medium, Modification 1 plus Vitamins Contains: microelements, 1/2 macroelements and vitamins Sucrose, cytokines and auxins # x 1L $ # x 10L $ # x 25L $ MS Medium, Modification 2 plus Vitamins Contains: microelemints, 3/4 macro elements and vitamins Sucrose, cytokines and auxins For all plant tissue culture and plant transformation reagents visit # x 1L $ # x 10L $ # x 25L $ MS Medium, Modification 3 plus Vitamins Contains: microelements, macro elements with 1/2 concentration of NH 4, NO 3 and KNO 3, and vitamins Sucrose, cytokines and auxins. # x 1L $ # x 10L $ # x 25L $ MS Medium with Van Der Salm Modification Contains: micro elements and macroelements with FeNaEDTA replaced by FeEDDHA Sucrose, cytokines, vitamins and auxins MS Medium with Finer and Nagasawa Modification Contains: microelements, macro elements with 1/2 concentration of NH 4 NO 3 and 1.6 x concentration of KNO 3 Sucrose, cytokines, vitamins and auxins # x 1L $ # x 10L $ # x 25L $ # x 1L $ # x 10L $ # x 25L $ transformation regeneration propagation

10 MS Medium plus Gamborg s B5 Vitamins Contains: Macro and Micro # x1L $ elements, vitamins Sucrose, cytokines and auxins # x10L $ # x25L $ MS Medium plus MES Buffer Contains: Macro and Micro # x1L $ elements, vitamins Sucrose, cytokines and auxins # x10L $ MES is non toxic for plant tissue and cells. # x25L $ MES is an excellent buffer for plant media because of a high buffer capacity and its ph range of MS Medium plus Nitsch Vitamins Contains: Macro and Micro # x1L $ elements, vitamins Sucrose, cytokines and auxins # x10L $ # x25L $ MS Medium with Shoot Multiplication Medium B New Contains: micro elements and # x1L $ macro elements with FeNaEDTA replaced by FeEDDHA # x10L $ Sucrose, cytokines and auxins # x25L $ BASTA (Glufosinateammonium) New Selective herbicide for plant transformation studies using nucleatide sequence of the # ml $ phosphinothricin Nacetyltransferase gene from Streptomyces virido chromogenes, Bar gene. Acts by inhibiting glutamine snthetase and thus blocks all nitrogen assimilation into the plant. 10% solution. Also known as gluphosinate FungiGone New FuniGone is bioworld s proprietary # ml $ reagent formulation This product will drastically reduce the growth of fungi in your tissue # ml $ culture applications, without affecting the plant tissue Supplied as 100X concentrate In vitro plant tissue culturetested. biomedia Plant Tissue and Cell Culture Media plantmedia.com 129 For In vitro lab use only Not for human use or consumption