Ultrasensitive Detection of Nucleic Acids and Visualization of DNA Through >165,000bp with a New PFGE Capillary Electrophoresis System

Transcription

1 Ultrasensitive Detection of Nucleic Acids and Visualization of DNA Through >165,000bp with a New PFGE Capillary Electrophoresis System Steve Siembieda, MS MBA VP Commercialization February 13, 2016

2 Fragment Analyzer Premier automation instrument Parallel capillary electrophoresis platform Developed to address key bottlenecks Unique design features to improve lab workflow

3 Unique Design Features The Fragment Analyzer Uses 12, 48 or 96 capillary arrays Switches between multiple gel matrices Holds up to three 96-well sample plates Runs the widest range of applications Is a flexible and dependable platform Processes data with a powerful analytical software PROSize 2.0

4 Unique Design Features Over 20 Reagent Kits Qualitative kits for PCR amplicon, DNA fragments, CRISPR gene editing Quantitative kits NGS library QC Genomic DNA and large fragments RNA and small RNA

5 Fragment Analyzer Products

6 Large Fragment Analysis DNF-464 Kit PacBio library sizing Normalization is required Recommended concentration is 1 ng/µl

7 Large Fragment Analysis DNF-464 Kit PacBio library sizing Normalization is required Recommended concentration is 1 ng/µl

8 Fragment Analyzer Femto PULSE

9 Fragment Analyzer Femto PULSE Built on the Fragment Analyzer frame Award winning parallel capillary array design Sensitivity to 1 fg/µl Separation of DNA through 165,000bp Nondenaturing gels

10 Fragment Analyzer Femto PULSE Addressing the changes in genomics discovery Push boundaries - enable discovery Single cell analysis Low quantity NGS library preparations Exosomes, small RNA, cfdna evaluation QC genomic DNA and large DNA fragments BAC clone sizing Foreign DNA in vaccines Biobanking Fresh, frozen and FFPE isolates Eliminate performance based qualitative tests

11 The Next Level of Separation Separate large DNA fragments Only automated pulse field parallel parallel CE electrophoresis instrument available One hour separation times Automated analysis

12 The Next Level of Separation Separate large DNA fragments Only automated pulse field parallel parallel CE electrophoresis instrument available One hour separation times Automated analysis

13 The Next Level of Detection Increased sensitivity detect fg/µl levels Optimized optics Increased power density on the detection window Better algorithm for data processing Modified gel chemistry Boundary breaking detection levels

14 ,600 17,700 21,100 23,450 41,770 50, ,650 Separation Through 165,000bp

15 Intact and Sheared gdna Sample provided

16 BAC Analysis 20 times faster than conventional PFGE methods

17 Detection Down to 1 fg/µl Serial dilution 1 fg/µl 300 bp fragment 30 fg/µl to 1 fg/µl Constant field conditions 1 fg/µl 2 fg/µl 4 fg/µl 7.5 fg/µl 15 fg/µl 30 fg/µl

18 Single Cell gdna Analysis Visualize a single cell of DNA Serial dilution from 250pg to 4 pg Non destructive method

19 Single Cell gdna Analysis Visualize a single cell of DNA Serial dilution from 250pg to 4 pg 32pg 16pg 8pg 4pg Non destructive method

20 Smear Detection Qualify low quantity libraries Serial dilution of a NGS library 100 fg/µl Serial diluted from 800 fg/µl to 100 fg/µl. Constant field electrophoresis

21 Ion Library Equalizer Kit Bead based solution to capture 100pM of library preparation Represents about 15pg/µL smear Not detectable on conventional instruments Visualize directly on Femto PULSE Why qualify these captures?

22 Ion Library Equalizer Kit Bead based solution to capture 100pM of library preparation Represents about 15pg/µL smear Not detectable on conventional instruments Visualize directly on Femto PULSE Why qualify these captures?

23 Ion Library Equalizer Kit Bead based solution to capture 100pM of library preparation Represents about 15pg/µL smear Not detectable on conventional instruments Visualize directly on Femto PULSE Why qualify these captures?

24 Iso-Seq RNA Library Preparation PacBio IsoSeq library preparation Produces 4 separate libraries 4 different transcript size ranges One RNA extraction

25 Breaking Barriers Femtogram level sensitivity Large DNA fragments qualification Rethink your analysis Cordova 5