Microbiology. Sampling, processing and cultivation

Transcription

1 Microbiology Sampling, processing and cultivation

2 Sicherheit Petri Dishes... Petri Dishes for Bacteriology Petri dishes from Sarstedt are made from highly transparent polystyrene and are heat-resistant to approximately 80 C for applications using hot agar. They are available in 35, 60, 92 and 150 mm diameter options. Their dimensional stability ensures optimal suitability for use in automated dish pourers as well as easy stacking. While versions with ventilation cams provide enhanced gas exchange, those without ventilation cams enable extended incubation times. Coloured dishes provide a variety of coding options. A Petri dish subdivided into two compartments is designed for parallel analyses under comparable conditions or the application of two different agar systems. In addition to round options, our range includes a square Petri dish for more efficient storage. Highly transparent polystyrene Stackable With and without ventilation cams Gamma irradiated options available Petri dishes for bacteriology From highly transparent polystyrene, heat-resistant to approx. 80 C ø/height Ventilation cams /bag /20 gamma-sterile with 100/ /16 without 480/ /16 with 480/ /16 gamma-sterile without 480/ /16 gamma-sterile with 480/ /15 gamma-sterile with 500/ /10 gamma-sterile with 500/20 Coloured Petri dishes with ventilation cams ø/height Colour /bag /16 red 480/ /16 yellow 480/ /16 blue 480/ /16 green 480/20 Petri dish, round, divided ø/height Ventilation cams /bag /16 with 480/20 Petri dish, square L x W x H 100 x 100 x 20 gamma-sterile Ventilation cams /bag without 160/4 2

3 Sicherheit DishRack... Air Sampler DishRack - The stacking solution for Petri dishes Convenient handling Stop knocking over stacks of dishes. Secure up to 88 dishes single-handedly with the DishRack. Each DishRack features retaining elements to accommodate 4 stacks of standard Ø 92/100 mm Petri dishes. Dishes can be removed from the rack at random, alleviating the laborious task of rearranging an entire stack. The DishRack is a valuable tool for multiuse at workstations, in incubators, or for sample storage. Durable and safe transport Elastic silicone strips securely hold dishes in the rack even if exposed to heavy shaking and movement during transport. The high-grade temperatureresistant plastic of the DishRack is largely resistant to most acidic and alkaline solutions. Organization The DishRack is perfectly suited to accommodate, transport, incubate and store dishes in logistic systems. DishRack facilitates and helps to organise individual work processes. Our range includes 5 different colours and exchangeable writing strips for customisation. For example, the green DishRack might be used to store all urine samples and the yellow to store stool cultures. Convenient handling Secure transport Organization Available in 2 sizes and 5 colours DishRack 50 for up to 52 plates Colour Height in cm neutral red yellow blue green 24 DishRack 80 for up to 88 plates Colour Height in cm neutral red yellow blue green 36 Air Sampler The GS 100 Air Sampler monitors ambient air quality for microbiological analyses in laboratories, hospitals and production facilities. The instrument operates according to the Anderson Air Sampler principle, aspirating ambient air through a jet plate while a micro processor controls and checks sampling. A thermal mass flow meter also precisely regulates the flow. While the air flow has been pre-set to 100 l/min, the inlet opening allows an aspiration speed of approximately 0.4 m/sec. Particles and microorganisms are transferred onto a standard Petri dish located underneath, without passing filters or nutrient solutions. Subsequently, the colonyforming units can grow directly on these dishes. Microbial count can then be carried out according to standard procedures. 100 l/min flow rate Autoclavable collection head Mains power pack or rechargeable battery Air Sampler Air Sampler GS 100, 230V Collection head GS Carrier case GS 100 3

4 Sicherheit POS PTS POS 720 Petri Dish Organization System The POS 720 and PTS represent important milestones in automation of microbiological laboratories with average to high sample throughput. In a fully automated process, more than 700 Petri dishes are labelled, stacked in sets and transported to individual workstations per hour. Labelling errors in microbiology laboratories are reduced and processing procedures are made simple and consistent, improving quality and competitiveness. Labour saving and easy to operate Reliable provision of all required Petri dishes Consistent barcode labelling and clear alphanumeric printing Dishes tracked throughout the process Additional labels for special media and agar available at the workstation POS 720 Petri Dish Organization System POS 720/PTS Power Mains supply Environment Adm. ambient temperature Max. relative humidity Dimensions Width x depth x height Weight Accessories Label printer Labels Capacity Petri dishes Print Throughput EDP link 230 V ± 10% / Hz / 400 VA +15 C to +35 C 80%, non-condensing 1,700 mm x 1,100 mm x 1,800 mm (height with assembled signal system) 200 kg, without Petri dishes Thermo direct printer with label dispenser and automatic spooling of excess paper Adhesive labels on rolls Supply: 10,000 pcs. / roll Format: 78 mm x 10 mm Material: Thermo Premium Top (other s are available on request) Adhesive: Permanent (other adhesives are available on request) All mm options (compatibility list available on request) Barcode and text symbols, customised layout Approximately 720 dishes/h Network connection to laboratory LANRS232/V24 Culture media dishes are automatically Printer and applicator print and attach the labels to the dish base Sorted Labelled/printed Stacked by specimen type Transported to workstations Dish stacker creates one stack per specimen type Dish with barcode and customised layout WOUND 4

5 Sicherheit Automation... for Microbiology PTS Petri Dish Transfer System The Petri Dish Transfer System transports the dish stacks pre-sorted by the POS 720 to the individual workstations. The free-standing system provides customised solutions and is adjustable in height within a defined range. Laboratory tables and benches can be conveniently positioned close to the PTS. A separating station with an integral scanner regulates the distance between the dish stacks. Exit points on the track ensure that stacks are correctly assigned to the relevant workstations. Dish stacks that cannot be identified by the scanner are transferred to an exit for error resolution. Layout example for PTS with three workstations (AP 1 AP 3) and exit for unidentifiable dishes PTS Petri Dish Transfer System PTS Power Mains supply AP 2 AP 1 AP V ± 10% / Hz / 322 VA Unidentified dishes Fehler Signal lamp for status control and safe entry to POS 720 Environment Adm. ambient temperature Max. relative humidity Dimensions Width x depth x height Weight +15 C to +35 C 80%, non-condensing Dependant upon customised design Dependant upon customised design Turntable with 15 magazines for 40 dishes each Separating station with scanner Feed track adaptable to any room layout Dish stack on its way to the workstation 5

6 Inoculation Loops Swabs Inoculation Loops, Needles and Spreaders Sarstedt s inoculation loops, needles and spreaders for single use are easy to handle, provide enhanced safety, minimise the risk of cross contamination, and save time. Heat sterilising in a Bunsen burner flame is no longer necessary between two inoculation processes, eliminating the spread of pathogenic aerosols. Flexible inoculation loops facilitate smears or inoculation in liquid media. 1 and 10 µl loops are colour-coded for easy identification. Sarstedt s inoculation needle is ideal for the transfer of individual colonies while the inoculation spreader serves to spread larger volumes across culture media. Time-saving, particularly for extensive test series Easy to use Superior safety Gamma-irradiated Gamma-irradiated inoculation loops, needles and spreaders from polystyrene Colour µl loop blue 10/peel pack, 1,000/case µl loop blue 48/peel pack, 1,920/case µl loop white 10/peel pack, 1,000/case µl loop white 48/peel pack, 1,920/case needle orange 10/peel pack, 1,000/case needle orange 50/peel pack, 2,000/case inoculation spreader blue 1/bag, 500/case inoculation spreader blue 4/peel pack, 500/case Swabs Sarstedt s swabs are ideal for the collection and transport of bacteriological, serological and cytological specimens. They are intended for use on intact skin and in natural body openings. The swabs are also suitable for health care monitoring in the food industry as well as for sampling from a variety of surfaces. In addition to short and long swab options, the range includes plastic or aluminium stems as well as versions with and without a transport medium. For extended transport distances or the transport of fastidious micro organisms we suggest using swabs with a transport medium. The addition of carbon to the medium of certain options serves to neutralise bacterial toxins and other inhibiting substances. Neutral swabs, sterile ø/tube length Stem /length Swab /101 plastic/85 cotton 500/bag, 500/case /155 plastic/135 viscose 100/bag, 1,000/case /155 aluminium/145 viscose 100/bag, 1,000/case 6

7 Swabs Swabs with Amies transport medium, sterile Transport tube and separate swab, individually wrapped in convenient peel pack, sterile Transport medium in accordance with DIN 58942, Part 4 TP-AL Suited for aerobic and anaerobic bacteria ø/tube length Stem / length Swab /155 plastic/145 viscose 500/case /155 aluminium/140 viscose 500/case Swab with Amies transport medium plus charcoal, sterile ø/tube length Stem / length Swab /155 plastic/145 viscose 500/case /155 aluminium/140 viscose 500/case Twin Swab To identify an infection caused by methicillin-resistant Staphylococcus aureus, a throat, nose and/or skin smear is followed by two diagnostic methods: molecular-biological analysis using PCR and a microbiological analysis. The Twin Swab allows the use of both diagnostic methods in one product, eliminating the need for a second smear and the risk of sample mix-up. The Stuart medium ensures stabilisation of the sample during transport. Ideal for double diagnoses Designed for throat, nose and skin smears Stuart medium for stabilisation during transport Individually wrapped, sterile Forensic Swab The new Forensic swab from Sarstedt is suited for the collection of both reference samples and evidence of DNA traces at crime scenes. The ventilation membrane in the tube base enables self-drying of the swab within the tube. Drying of the swab outside the tube as in standard systems becomes unnecessary, thereby excluding the risk of mix-up and contamination. The EtO sterilisation method specially developed by Sarstedt to comply with the requirements of forensic analysis, including most stringent purity conditions in the production process, ensures the absence of DNA contamination. DNA-free EtO-sterilised Self-drying within the tube Individually wrapped, sterile Twin swab ø/tube length Stem / length Swab Transport medium /155 plastic/135 viscose Stuart medium, liquid 50/bag 500/case Forensic swab /102 polystyrene/93 viscose 1/paper-plastic pack 100/inner box 500/case 7

8 Sicherheit Faeces... Faeces Diagnostics Faeces tubes from Sarstedt enable a clean and easy collection of faeces samples. Various sizes and sampling scoops are available, including an option to collect a defined faeces volume of 1 ml, approx. 1 g. The range includes sterile, labelled or opaque options. On request, faeces tubes with customised label are available at a minimum ordering quantity of 15,000 pcs. Screw cap faeces tubes ø/tube length Tube /107 PP sterile /107 PP non-sterile /101 PP sterile /101 PP non-sterile /76 PP sterile /76 PP non-sterile /76 PP /76 PP /76 PP, white sterile, labelled non-sterile, labelled non-sterile, labelled Faeces tube with flat base and push cap ø/tube length Tube /75 PS sterile /75 PS non-sterile 500 Screw cap tube for the collection of a defined faeces sample This faeces tube enables a clean and simple separation of a defined faeces sample of 1 ml, approx. 1g. The spoon has a capacity of 1 ml and any excess is removed with an enclosed spatula. The spoon is fixed to the cap and sits halfway down the tube when capped. This construction enables centrifugation and separation of a supernatant without disturbance upon opening. ø/tube length Tube /101 PP Faeces tube incl. spatula 1, ml and 70 ml Faeces containers The 25 and 70 ml tubes are made from a durable white polypropylene. The brown screw cap includes an integral sampling scoop. While containers are primarily used for the collection of faeces samples for pathology, they are also very convenient for the collection of grain and soil samples. ø/length Container Volume in ml /54 PP /55 PP 70 White, with brown cap and label White, with brown cap and label

9 Sicherheit Urine Transport... System Urine Diagnostics Sarstedt s range of urine collection products includes two systems: The Urine Monovette works using the classical aspiration principle where urine is collected by placing the suction tip into a urine sample and withdrawing the piston (Fig. a). The V-Monovette Urine is a vacuum system for enclosed urine transfer. This system provides enhanced hygiene and convenient handling for patients and users. It is not necessary to open a urine cup or container to fill the tube so that contamination is practically excluded (Fig. b). Both systems are available with a boric acid preparation to stabilise the microorganisms contained in the urine for up to 48 hours at room temperature. Please refer to our brochures Urine Analysis and V-Monovette Urine with additional products for urine analysis. Urine culture * * Accessories Urine-Monovette, printed, with stabiliser, ind. wrapped, sterile V-Monovette Urine with stabiliser, round base V-Monovette Urine with stabiliser, round base (Fig. b) Tube with screw cap (red) assembled, with paper label, with boric acid, PP Cup with assembled screw cap, sterile, with safety label Cup with integral transfer device, assembled lid, sterile, with safety label Volume in ml ø/length (Fig. a) 10 15/ / / / Volume in ml ø/length / /73 * Available 3. quarter /bag 200/case 5/bag 200/case Transport System Our range includes a complete packaging system of mailing containers/ tubes and boxes for the transport of urine and faeces samples. Tested by the Federal Institute for Material Research and Testing (BAM) in Germany, the system has been approved to comply with the requirements of P650 Instructions for Class UN 3373 of the ADR, RID, ICAO and IATA. These instructions require a transport packaging consisting of 3 components: the primary receptacle, the secondary packaging and a protective outer packaging. As established by IATA, Sarstedt s primary receptacles (faeces tubes, Urine Monovette ) are designed to withstand, without leakage, an internal pressure difference of at least 95 kpa (0.95 bar) for the safe and optimal transportation of diagnostic specimens in accordance with relevant requirements. Please refer to our brochure No Transport and Mailing Systems for detailed information. 9

10 Sicherheit Disposal Bags... Disposal Bags Sarstedt s disposal bags are ideal for the containment and disposal of contaminated laboratory and hospital consumables. A robust 50 µm thick polypropylene bag with a durable, wide base seam ensures a good resistance to tear and puncturing. However, in view of the inherent injury risk, sharp or pointed objects must never be placed into the bags. Sarstedt s disposal bags are suited for hot steam sterilisation in the autoclave from 121 C (1) to approx. 134 C (2). For a complete steam sterilisation, the disposal bags must always be open during autoclaving. The range includes a variety of sizes and colours as well as options with Bio Hazard print. Durable (50 µm) for improved safety Reduction of waste volume Suitable for autoclaving at 121 C (1) to approximately 134 C (2) Table rack for disposal bags Steel rack coated with epoxy resin 1 rack including dispenser box with bags ( ) Disposal bags Opening dimension x length (mm) Bag colour Bag print Print neutral yellow yes no red blue * 200 x 300 (1) 100/1, x 500 (2) 50/ x 500 (1) 50/ x 500 (1) 50/ x 500 (2) 50/ x 780 (2) 50/ x 780 (1) 50/ x 780 (1) 50/ x 780 (2) 50/ x 780 (2) 50/ x 780 (1) 50/ x 780 (1) 50/ x 780 (2) 50/ x (2) 50/ x (2) 50/ x (2) 50/150 *Disposal bag for table rack in convenient dispenser box 10

11 Sicherheit MegaBlock... Rack Series 20 MegaBlock The 96 well MegaBlock meets all important requirements for processing sample volumes from 1.2 to 2.2 ml in automated systems or for storing large quantities of backup samples Tape/mat covers are included in the range. Products are manufactured under stringent clean room conditions and are protected against contamination. MegaBlock 1.2/2.2 ml, PP Each well 100% leak-proof tested Ideal for backup storage of pharmaceutical substances Ideal for DNA isolation Solvent-resistant to DMSO Suited for enzyme assays and tissue culture applications Round bottom wells with alphanumeric coordination system Ideal for long-term sample storage Autoclavable* MegaBlock 1.2 ml, transparent, PS Each well 100% leak-proof tested Ideal for long-term storage of blood samples Pierceable mat cover Transparent for superior visual control Round bottom wells with alphanumeric coordination system MegaBlock Volume/Material round wells 1.2 ml PS transparent round wells 1.2 ml PP square wells 2.2 ml PP 32 Mats and sealing tapes for MegaBlock Mat cover for 1.2 ml MegaBlock, pierceable, compatible with and Mat cover for 2.2 ml MegaBlock, compatible with pcs./inner box 10 pcs./bag pcs./inner box 10 pcs./bag Aluminium tape, opaque 100 tapes/inner box Acetate tape, transparent 100 tapes/inner box Rack System for Faeces and Urine Samples Four different racks to accommodate a range of diameters from 17.2 to 26 mm are available for spacesaving and systematic backup storage of faeces and urine samples. The high-grade, break-resistant is durable and can be autoclaved at 121 C*. Racks can be easily disassembled for cleaning. A version with side pocket for documents is also available. A cover compatible with this rack protects open tubes against contamination. * Please refer to our general catalogue for information on additional rack series. Rack Series 20 Aperture diameter Outer dimensions Application (examples) top centre bottom WxHxL x 60 x 327 Tubes up to 25 mm Ø x 60 x 327 Tubes up to 21 mm Ø x 55 x 257 Tubes up to 17 mm Ø, all S-Monovettes x 55 x Cover in brown, translucent 62 x 72 x 256 Rack with side-pocket for documents, tubes to 17 mm Ø, all S-Monovettes Compatible with rack ; the cover protects open tubes against contamination * Important notes on autoclaving: Products made from PP can be autoclaved up to 121 C without significantly impairing their mechanical properties. Users are to test for themselves if autoclaving may have any effect on other characteristic product features so as to influence the individual application concerned. 11

12 You may also be interested in the following subjects... Urine Analysis POS 720/PTS Urine Monovette Urine Monovette with stabilizer UriSet 24 Urine Containers Urine Cups Automation in Microbiology : : V-Monovette Urine! W NE The vacuum system for enclosed urine transfer : Transport and Mailing Systems PCR & Molecular Biology Cell and Tissue Culture For transportation of diagnostic samples and blood products Certified High-Quality Products for PCR, Molecular Biology and Research Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling sterile non-pyrogenic DNA-free RNase-free ATP-free The complete product range for mailing and transport requirements Liquid Handling Note: The products shown in this brochure may not be available in all countries! Safe and compliant with transportation guidelines 1 : : CryoPure Storage System... Energizing your Cryopreservation : Screw cap micro tubes Forensic-Swab in a transport tube with ventilation membrane For transport, storage and sample preparation Maximum purity for optimal safety in preanalytics Efficient and reliable cryopreservation Reliable quality and versatility The new Forensic-Swab from Sarstedt is suited for the collection of both reference samples and evidence of DNA traces at crime scenes. The ventilation membrane in the tube base enables self-drying of the swab within the tube. Drying of the swab outside the tube as in standard systems becomes unnecessary, thereby excluding the risk of mix-up and contamination. The EtO sterilisation method specially developed by Sarstedt to comply with the requirements of forensic analysis, including most stringent purity conditions in the production process, ensures the absence of DNA contamination. DNA-free EtO sterilised Self-drying within the tube Individually wrapped, sterile Technical modifications reserved. Ordering Information : SARSTEDT AG & Co. P.O. Box D Nümbrecht Phone Fax info@sarstedt.com : Tube length Stem Swab / 102 Polystyrene / 93 Viscose 1 piece / paper-plastic 100 pcs. / inner box 500 pcs. / outer box : ! W NE