MICROBIOLOGY. Course Description: A study of microorganisms and the major areas of bacteriology: medical, industrial,

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1 Fall 2002 Course Syllabus BIOL 420 MICROBIOLOGY Instructor: Jay Brewster, Ph.D. Office (RAC120): Home: Office Hours: Tues., Thurs. 8-9 AM Lecture: KSC225 MTR 10-10:50 PM Laboratory: KSC330 MT 12-1:50 PM Teaching Assistants: TBA Course Description: A study of microorganisms and the major areas of bacteriology: medical, industrial, food, agricultural, and sanitation. Course Objectives: The central objective of this course is to instill within the students: 1) an understanding of the origin of microbiology and how it has broadened into a wide array of specialized career fields. 2) a knowledge of the metabolic and structural diversity microbes display and how that diversity benefits their survival in varied environments. Recent advances in technology that have benefited the study of microbial cell biology will be discussed. 3) a knowledge of the taxonomy of microbiology and a recognition of why classification is critical to diagnostics and epidemiology. Human disease as a result of viral, fungal, and bacterial infection will be identified. 4) a foundational understanding of immunology, the cellular and humoral response, and the differentiation of stem cells into mature leukocytes. 5) a competence with laboratory techniques used in the isolation, culture, characterization, and classification of microbiological specimens. 6) a recognition of the political, humanitarian, and ethical considerations faced by wealthy nations in light of worldwide disease. Students will discuss their perspective and opinions regarding world health issues. Required Textbooks: Prescott, L., Harley, J.P., and D. Klein, Microbiology (Fifth Edition), McGraw-Hill, Benson, H., Microbiological Applications (8th Edition), McGraw-Hill, 2002.

2 Course Policies: Attendance in both the lecture and laboratory sections is required. Excused absences must be presented before class to be considered. Three unexcused absences from lecture will result in a reduction in your final grade by one letter. One unexcused absence from laboratory will result in a reduction of the laboratory component of your final grade by one letter. No credit or make-up will be offered for students missing class with no prior discussion with the instructor. No late work will be accepted for credit, but may be handed in for evaluation. Reaction and Review Papers: Two reaction papers are required from the assigned reading list. Each reaction paper will include an introduction (background, relevance of subject material), a summary of the article (main topic, important points), and a discussion of your reaction to the material. These will be no more than 2 pages in length (single-spaced). Reaction paper due dates: TBA One review paper is required from each student covering a topic in microbiology. Each student must get approval for the chosen topic from the instructor (before October 30). Review papers will be a comprehensive evaluation of the chosen topic using information from relevant books, current journal articles, and current review articles. Students will be expected to research the topic carefully and perform literature searches through library or internet sources. Internet resources should be used cautiously as web-sites are not considered a reliable source of information. Use these resources for general information, and as a guide to peer-reviewed resources. Review papers that display a lack of support from scientific journals will be marked down. The review paper will be 8-12 pages in length (double-spaced). All sources will be referenced in the text and listed in an alphabetical bibliography. The review papers will be turned in before November 18.

3 Laboratory: Laboratory sessions will begin promptly at the specified time. Each laboratory session will begin with a quiz over the previous week s laboratory material and any introductory for that week s exercise. Students arriving late will not be allowed to take the quiz. Safety in the microbiology laboratory is a primary concern. Students will wear laboratory coats at all times and gloves when directed. No food or drink is allowed in the laboratory area. Each student is to maintain a bound notebook for recording laboratory experiments. The write-up for each weeks exercises will include; 1) an introduction (written before class), 2) notes from lecture preceding laboratory exercise (given at the start of the lab session), 2) a materials and methods section providing a detailed description of supplies, and the protocols that were employed, 3) a results section, describing the progression of the experiment and the data that resulted (sketches, descriptions, and raw data will all be included here), 4) and a discussion/conclusion section. Notebooks will be graded for content, organization, and formatting (date and signature on each page). Periodic checks of notebooks (unannounced) should be anticipated. Warning: Natural Science s laboratories contain and certain class experiments or procedures will expose you to chemicals known to the state of California to cause cancer, birth defects, and other reproductive harm at levels which require a warning. For more information, contact your instructor or the Office of Regulatory Affairs at extension Points Available: Exams (3 X 100) 300 Final Exam 200 Reaction Papers (2 X 25) 50 Oral Presentation 20 Review Paper 80 Group Project 50 Laboratory Quizzes 120 Bacterial Unknown ID (2 x 25) 50 Laboratory Notebook and Manual 100 Total Possible ~ 970 Grading Scale: Percentage Letter Grade 94% A 90% A- 87% B+ 83% B 80% B- 77% C+ 73% C 70% C- 67% D+ 63% D 60% D- etc.

4 Web Resources: Medline/Pubmed (literature searches): National Center for Biotechnology Information (NCBI): Centers for Disease Control and Prevention (CDC): National Institutes of Health (NIH): American Society for Microbiology: Landmarks of Microbiology (ASM): Society for General Microbiology: HIV/AIDS links - University of Washington (Mullins): Bugs in the News! : Cells Alive! (great links): Stalking the Mysterious Microbe (for children): Microbe World (for the general public):

5 BIOL Fall 2002 Schedule Week of: Topic: Text Readings: August History, Relevance of Microbiology Lecture: Chapter 1,2 Laboratory: Microscopy Exercise 1-5 (Meet only on Tuesday) September 2 Labor Day (no class, no lab on Monday) 3-6 Prokaryotic cell structure and function Lecture: Chapter 3 Laboratory: Survey of Microorganisms Exercise 5-7, 9 (Meet only on Tuesday) 9-13 Eukaryotic cell structure and function Lecture: Chapter 4 Laboratory: Bact. Morphology Exercise Microbial Nutrition, Growth and Control Lecture: Chapters 5,6,7 Laboratory: Staining Techniques Exercise Microbial Metabolism Lecture: Chapters 8,9,10 26 Exam I Laboratory: Pure Culture, Hand Scrubbing Exercise 21, 44 October Sept. 30- Oct 4 Microbiology of Food Industry Lecture: Chapter 41 Laboratory: Anaerobes, Quantification Exercise 22, Microbial Genetics Lecture: Chapters Laboratory: Environmental Stress Exercise Viruses and Prions Lecture: Chapters 16, 17 Laboratory: Bacteriophages Exercise 28, 30

6 21-25 Microbial Taxonomy Lecture: Chapter Exam II Laboratory: Disinfectants Exercise 40, Nov. 1 Gram Negative Bacteria Lecture: Chapter Laboratory: Unknowns Staph, Strep Exercise 78, 79 November Gram Positive Bacteria Lecture: Chapter 23, 24 Laboratory: Unknowns Staph and Strep Exercise 78, Fungi and Molds Lecture: Chapter 25 Laboratory: Unknowns Gram - and Urinary Exercise 80, Bacteria, Viruses and Human Disease Lecture: Chapter Exam III Laboratory: Unknowns Gram and Urinary Exercise 80, 81 (27-29 THANKSGIVING BREAK) December Dec. 2-6 Bacteria, Viruses and Human Disease Lecture: Chapter Dead Week Lab: Trip to Sepulveda Veterans Administration Medical Center Dr. John Garcia (medical microbiologist) 9-12 FINALS Final Exam - Tuesday, December 10, 10:30 A.M. - 1:00 PM