C125-E008I RF-5301PC. Shimadzu Spectrofluorophotometer

Transcription

1 C125-E008I RF-5301PC Shimadzu Spectrofluorophotometer

2 World s Highest Level Performance and Windows TM Software High-Sensitivity (S/N Ratio Over 150) The essence of fluorescence analysis is sensitivity. The high-throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital circuit to provide the highest level S/N ratio attainable. High-Speed Scanning High-speed scanning up to 5,500nm/min allows you to measure a spectrum in seconds. And since monochromator slewing is about 20,000nm/min, setting of two or more wavelengths can be performed quickly and easily. Versatile Sample Compartment Size The sample compartment measuring 140mm wide, 170mm deep and 140mm high, enables use of micro cells, high-sensitivity cells, LC flow cells, etc. for a wide range of applications. Built-In Performance Checks When the instrument is switched ON, operating conditions of the spectrophotometer are automatically verified. Separately, noise level (S/N ratio) and light source (Xenon lamp) usage are built-in features to help maintain the instrument in its optimum condition, providing absolute confidence in the quality of the data. 2

3 Open Up a New Spectrum of Fluorescence Analysis. Windows-Based Workstation A PC directly controls the instrument for data acquisition and processing. The Windows TM friendly operating environment allows you to perform measurement, data processing, editing and recording in one continuous operation with a click of the mouse. Using the Copy Graph function, measurement data or spectra may be easily transferred to word processing or spreadsheet software for preparation of documents or additional calculations. From Routine Analysis to Research Employing the Highest Level of Sensitivity in the World! Features of Fluorescence Spectrometry Tens to thousands of times higher sensitivity than absorption spectrometry High selectivity (combining excitation and fluorescence wavelengths allows measuring even mixtures) Life Sciences Amino acids, peptides, proteins, fats, sugars, vitamins, coenzymes, amines, etc. Qualitative/quantitative analysis Enzyme reaction research Research of reaction mechanisms through analysis of substrates, coenzymes, and proteins (tryptophan and tyrosine residue) Antigen-antibody reactions Research of protein-protein, protein-nucleic acid, and protein-sugar interactions Analysis of intracellular ions (such as Ca 2+ ) using a fluorescence probe (such as Fura-2) Analysis of neurotransmitters (such as catecholamines) Research of nucleic acid hybridization Research of biomembranes Food Products Amino acids, proteins, fats, carbohydrates, vitamins, coenzymes, antibiotics, food additives (such as flavorings, seasonings, and antioxidants), cooking oil (such as vegetable oils and animal oils), and pesticides (carbamate, organophosphorus, and 1- naphthylacetic acid) Food ingredient analysis and quantitative analysis Inspections for residual pesticides Chemicals / Environment Organic, inorganic, and polymer compounds (such as fibers, plastics, and rubbers) Trace analysis Materials science research Photochemical reaction research Polymer research Optical materials research Assessment of atmospheric, water, and soil pollution Such as the analysis of polycyclic aromatic hydrocarbon compounds Crude oil and coal analysis 3

4 Windows-Based Software Provides Powerful Support Windows-Based Control 3 Measurement Modes SpeedBox TM Copy and Paste Wide Selection of Data Processing Functions Enables comprehensive management, from measurements to data analysis and report creation/output. Online help is provided as well. Select from 3 measurement modes according to the sample Spectrum Measurement Mode To measure spectra, simply click the [Start] button. Displays up to 10 spectra in the same window and performs calculations interactively. Freely zoom in or out using the mouse. Shimadzu's proprietary radar function allows viewing all spectra with a single click. Quantitative Measurement Mode Automatically creates calibration curves from measurement of standard samples. To quantitate unknown samples, simply click the [Read] button. PopUpScan TM allows confirming spectra while quantitative measurements are still in progress. Quantitation method: Peak height Calibration curves: 1st to 3rd order equations Mean value calculation function Time Course Measurement Mode High sensitivity, a stable baseline, and a 20-bit high-resolution A/D converter ensure reliable detection of small changes in samples. The activity value calculation function supports even enzyme research. 4

5 for Sample Analysis and Data Processing One-Click Operation SpeedBox TM Create buttons for frequently used functions, to execute the functions with a single click. Operators can freely customize button shapes, locations, and other features. Compatible with Commercial Software Applications Copy & Paste Data can be copied and pasted to Windows-based applications, such as word processing and spreadsheet software, which allows creating sophisticated reports. The ASCII conversion function allows transferring data to spreadsheet software. Simple and Powerful Extensive Data Processing Functions Data processing functions below can be used to correct data, extract hidden information, and so on, from the spectra obtained. Four basic arithmetic operations Smoothing Area calculation Differentiation Peak detection Activity value calculation All functions can be operated easily and visually using the mouse. 5

6 Fast and Smart Monochromator and High Expandability S/N Ratio over 150:1* Max. Scan Speed of 5,500 nm/min Wavelength Slewing Speed of 20,000 nm/min Search Function Self-Diagnostic Function High-sensitivity and high-speed scanning provides quick and easy measurements. The search and self-diagnostic functions allow performing measurements without time-consuming processes. * Signal-to-noise ratio is from a Raman peak for distilled water with an excitation wavelength of 350 nm and bandwidth of 5 nm. High Sensitivity S/N Ratio over 150:1 A bright optical system featuring a blazed holographic grating and digital signal processing enable ultra trace analysis at signal-to-noise ratios over 150:1. Note: S/N ratio is measured for the Raman line of distilled water Excitation wavelength Bandwidth 0-98 % response 350nm 5nm 2.0sec Note: For a bandwidth of 10 nm, the S/N ratio is equivalent to over 600:1. Detects Optimal Excitation and Fluorescence Wavelengths Search Function Simply click the [Search] button. Automatically detects optimal excitation and fluorescence wavelengths. Automatically determines fluorescence from Rayleigh scattering, Raman scattering, 2nd-order light, etc. Makes time-consuming wavelength settings easy. Enables High-Speed Measurements Max. Scan Speed of 5,500 nm/min An advanced direct wavelength drive mechanism provides spectra with minimal distortion, even at high speeds. A maximum scan speed of 5,500 nm/min enables high-speed spectra monitoring. Fast 20,000 nm/min wavelength slewing speed improves measurement efficiency. Automatic Instrument Performance Management Self-Diagnostic Function Automatically checks the operating status of the drive system when power is switched ON. Excitation slit Excitation monochromator Fluorescence slit The software automatically performs time-consuming instrument management and performance checks, making it possible to use the instrument in an optimal state at all times. Manages lamp usage hours. Automatically measures and calculates S/N ratios. Fluorescence monochromator Zero setting 6

7 Provide Powerful Support for Measurement and Analysis Large Sample Compartment High Degree of Expandability Systems can be tailored to samples and measurement methods. Easy to Use, Large Size Large Sample Compartment A large sample compartment provides plenty of space, improving ease of use. A wide variety of optional equipment can be attached. Various types of cell holders Various types of flow cell units Polarizer attachment Allows Configuring a Variety of Systems High Degree of Expandability Outputs analog signals of fluorescence intensity. Can be connected to a Chromatopac integrator. Enables chromatographic measurements. Note: Analog signals are output at a full scale of 1 V. Systems can be configured to samples and measurement methods. 7

8 Wide Variety of Optional Accessories Provides Powerful Support for Measurement and Analysis Intracellular Calcium Measurement System This measurement system uses a fluorescence probe to measure realtime changes in concentrations of Ca 2+, H +, and other intracellular messengers. Measures up to 4 wavelengths, allowing measurements using multiple fluorescence probes. High-speed switching between wavelengths enables switching from 340 nm to 380 nm in a minimum 0.5 seconds, using a Fura-2 probe. Displays real-time changes in fluorescence intensity (maximum of 4) and ratios (maximum of 4) during measurements. Support for quantitation using single-wavelength quantitation, two-wavelength quantitation, and calibration curves enables measurement using Fluo-3, Fura-2, BCECF, and other fluorescence probes. Compatible with Window XP Professional (SP2) and Vista Business. System Contents Part Name RF-5301PC Spectrofluorophotometer Intracellular Ca 2+ Measurement Software (Super Ion Probe) Constant-Temperature Cell Holder with Stirrer Constant-Temperature Water Circulator Syringe Adapter Computer and printer Part Number

9 Options NTT-2200P Constant- Temperature Water Circulator P/N Constant-Temperature Single Cell Holder with Stirrer P/N (100V) P/N (120V) P/N (220/240V) High Sensitivity Cell Holder P/N Circulates temperature-controlled water to a constant-temperature cell holder. -Temperature range: Ambient + 5 C to + 80 C -Temperature control precision: ±0.05 C~ -Max. pumping rate: 27/31 L/min, 9.5/13m (50/60Hz) -External circulation nozzle: 10.5mm OD (both outlet and return) -Tank capacity: About 10L (9L during use) -Safety features: Detection of over-temperature of Upper or Lower limits, Detection of heater wire malfunction, Protection of heating too little circulating water, Detection of sensor malfunction, Independent over heat protection, Over current circuit protector -Standard accessories: Lid with handles, Rubber hose (4m, 9mm ID), Hose clamps (4pc.), Instruction manual -Dimensions: 270W x 560H x 400D (mm) -Power requirements: 100VAC, 1250VA, with 1.7m power cord and grounded plug Note: Piping to Constant-Temperature Cell Holder (P/N xx) is possible by the hose (9mm ID). Keeps the sample at the desired constant temperature during measurement by circulating temperature-controlled water (5-70 C) around the sample cell. The stirring option has variable speed control and the minimum sample volume is 2mL. Effective for fluorometry of suspended cell samples. Note: Does not include the Temperature Controlled Water Bath. Not applicable to samples having a specific gravity greatly different from that of the solvent. Note: The hose joint is applied to the 6-9mmID tube. Constant-Temperature Four Cell Holder P/N Keeps four sample cells at the desired constant temperature via connection to a water bath. Note: Requires the Temperature Controlled Water Bath. This cell holder enhances the sensitivity two to three times or more. This is recommended for low concentrations. The excitation and emission beams are repeatedly reflected through the sample to increase the excitation efficiency. Solid (Powder) Sample Holder P/N Provides a means to examine the fluorescence of a solid sample. The angle of the holder is designed to limit the reflected excitation beam from the emission monochromator. Standard accessories include a sample holder, quartz plate, powder sample plate, spacers and a sample fixing bar. Cutoff filters are included to reduce scattering further (UV-31, UV-35, UV-39, UV-Y43, UV-Y45, UV-Y47). Rectangular Flow Cell, 120μL P/N Used for fluorescence monitoring of effluents in column chromatography. Made of fused silica. Requires adapter (P/N ). 9

10 Options Polarizer Attachment (for UV/Vis region) P/N Polarizer Attachment (for Vis region) P/N Quartz Cell Polished on Four Sides P/N Non-Fluorescent Cell P/N Flow Cell Unit (120μL) for LC Application P/N Measurement of fluorescence polarization provides information on the size, fluidity and environments of fluorescent molecules. Permits reliable measurement of polarization characteristics over a wide wavelength range from 240nm to 800nm. Micro Cell Holder with Micro Cell P/N Provides a 10mm square nonfluorescent cell, polished on four sides. This cell is recommended for trace analysis particularly when low UV wavelengths are used for excitation. Does not fluoresce when excitation wavelengths from 240 to 260nm are used. Flow-Thru Cell Unit for HPLC (12μL) P/N The square micro-flow cell has an inner volume of 120μL and is made of silica to ensure minimum light scattering. The inlet and outlet tubes are both made of vinyl resin and are 0.5mm I.D. and 2mm O.D. R Photomultiplier P/N Permits measurement of samples as small as 400μL in volume. The cell is installed in an adapter of the same dimensions as an ordinary 10mm square cell, so it can be placed in the standard cell holder. With this attachment, the RF-5301PC is used as a highly sensitive spectral fluorescence monitor in HPLC. Free selection of the excitation and emission wavelengths permits selective detection. The mobile phase stream can be stopped for spectral measurement, so that chromatographic peaks can be qualitatively determined with higher reliability. The flow-thru cell is a square type with an inner volume as small as 12μL and is made of silica, ensuring minimized light scattering. Extends the measuring wavelength range from 750 to 900nm. Filter Set P/N Includes 8 filters: two broad-band interference filters U-340 and B-390 for isolation of excitation wavelengths of and nm, respectively. Six cutoff filters: UV-29, L-41, Y-46, Y-50, O-56 and R-60 with approximately 50%T at 300, 420, 460, 500, 560 and 600nm, respectively. 10

11 8mm dia. Test Tube Holder P/N Bottom Rise Sample Stage P/N Sipper Unit 5300 P/N Holds a test tube, 8mm outer diameter and 45mm-100mm long. The minimum sample volume required is 400μL. Raises the sample cell position in order to reduce the minimum sample volume to 1.5mL. Aspirates solution samples using a peristaltic pump driven by a stepping motor, to be measured successively. Minimum sample volume is 2mL, linked operation with the ASC-5 Auto Sampler possible. 12mm dia. Test Tube Holder P/N Spectra Correction Accessory P/N Used to correct excitation and emission spectra in the wavelength region from 250nm to 600nm, using rhodamine solution as the reference (quantum counter). ASC-5 Auto Sample Changer P/N xx Holds a test tube, 12mm outer diameter and 60mm-100mm long. Builds an automatic multi sample spectrophotometry system in combination with a sipper unit. Up to 100 test tubes are set together on the rack. The aspirating nozzle is programmable to move X, Y and Z (vertical) direction. 11

12 JQA-0376 Founded in 1875, Shimadzu Corporation, a leader in the development of advanced technologies, has a distinguished history of innovation built on the foundation of contributing to society through science and technology. We maintain a global network of sales, service, technical support and applications centers on six continents, and have established long-term relationships with a host of highly trained distributors located in over 100 countries. For information about Shimadzu, and to contact your local office, please visit our Web site at SHIMADZU CORPORATION. International Marketing Division 3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo , Japan Phone: 81(3) Fax. 81(3) URL The contents of this brochure are subject to change without notice. Printed in Japan ANS