CONTENTS. Introduction to Sequenom Overview MassARRAY System. Publications xR Sequenom Bioscience. All rights reserved.

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1 CONTENTS Introduction to Sequenom Overview MassARRAY System iplex Biochemistry Publications

2 Sequenom GmbH - brief history Founded in 1994 as Sequenom GmbH in Hamburg (DE) By Hubert Köster and Charles Cantor Hamburg site serves the European, Middle East and African user base

3 B Sequenom Inc. History In 1996 Sequenom Inc. was formed in San Diego, US Commercial Launch of MassARRAY in Q IPO February 1, 2000

4 MassARRAY Features High-level multiplexing, PCR amplify up to 40 loci (plex) in single well, microtitre plate No special primer, no label Same plate throughout process 5 µl PCR reaction 10 ng DNA per reaction Results are very sensitive, quantitative and cost effective Requires sequence to be approximately known in advance

5 Genomic Marker Discovery and Translation: Common Tools Marker Number DISCOVERY CONFIRMATION to ~ to >10 3 Sample Throughput

6 MassARRAY -Detection Principle MALDI-TOF MS (Matrix-assisted laser desorption/ionization ): Most accurate and sensitive DNA analysis by time-of-flight detection of molecular mass damp = Da dcmp = Da dgmp = Da dtmp = Da MMBT05728 UEP.MMBT01167 CT T C CT UEP.MMBT10830 CT T UEP.MMBT05641 C CT UEP.MMBT Mass

7 Genotyping Assay (iplex ) Chemistry.

8 Genotyping Assay (iplex ) Chemistry.

9 Genotyping Assay (iplex ) Chemistry.

10 Typical spectrum (36 plex)

11 High and Low Frequency Mutations Quantitative analysis 55% mutation frequency 5.5% mutation frequency

12 Typical MassARRAY Assay Workflow Isolate DNA or RNA Obtain primers Design assays Select markers PCR and Base Extension (~8 hours) Prepare SpectroCHIP (~20 minutes) Analyze with MALDI-TOF (30 minutes 1 hour).

13 Typer Software Traffic Light Histogram Cluster Plot Chip & Assay Summary Error Checking Multiple Plates can be Analyzed in Parallel Spectra Overlay

14 MassARRAY Sequenom Value MassARRAY in Agricultural Genomics Value in Agricultural Genomics Discovery Marker Development Validation Sample Screening Commercialization.

15 Physical Tracking vs. Genetic Tracking Using this same panel investigators were able to use the DNA evidence to confirm that the BSE-affected cow in the 2003 Washington state case was of Canadian origin. SAN DIEGO, Jan. 12 /PR Newswire- FirstCall/ -- SEQUENOM, Inc. (Nasdaq: SQNM) today reported that the origin of the recent case of bovine spongiform encephalopathy (BSE), or mad cow disease, was determined using the Company's proprietary MassARRAY (TM) technology. Mike Heaton, et al., J. Am. Vet. Med. Assoc (8):1311-4

16 Barley Variety Identification Genotyping Courtesy Robert Henry, SCU, Australia

17 SNP Validation in a Highly Polyploid Species 454 sequencing of 307 PCR amplicons of the highly polymorphic sugar cane SNPs were considered as candidates if the minor allele was present in at least 2 sequences and at a frequency of at least 4% From 225 candidate SNP sites tested, 209 (93%) were validated as polymorphic using the Sequenom MassARRAY

18 The Saccharum officinarum L (sugarcane) genome Saccharum officinarum Saccharum spontaneum Grivet L, Arruda P.: Sugarcane genomics: depicting the complex genome of an important tropical crop; Curr Opin Plant Biol Apr;5(2):122-7

19 iplex Gold Assay Throughput by SNP Number From 5,000 to 230,400 genotypes per day on the MassARRAY Analyzer 4 Chips Plex ,216 46,080 92, , ,824 69, , , ,360 76, , , , , , ,800 8h workday: 10h workday: 8 SpectroCHIPs per day 10 SpectroCHIPs per day 19.

20 Thank you!.