COURSE DESCRIPTION IMMUNOLOGY

Transcription

1 COURSE DESCRIPTION IMMUNOLOGY 3RD YEAR FIRST SEMESTER DEGREE: BIOTECHNOLOGY MODALITY: ATTENDANCE IS REQUIRED ACADEMIC YEAR 2016/2017 FACULTY OF PHARMACY

2 1.- COURSE/SUBJECT: 1. COURSE/SUBJECT IDENTIFICATION Name: Immunology Code: e301 Year (s) course is taught: 3º Semester (s) when the course is taught: 1º Type: Compulsory subject ECTS of the course: Hours ECTS: 25 Language: English Modality: on campus Degree (s) in which the course is taught: Biotechnology School which the course is taught: Pharmacy 2.- ORGANIZATION OF THE COURSE: Department: Area of knowledge: Immunology 2. LECTURERS OF THE COURSE/SUBJECT 1.-LECTURERES: Responsible of the Course CONTACT Name: Carolina Hurtado Marcos Phone (ext): 91372(4721)-91372(4784) Office: Teaching and Research profile Research Lines 219, C PhD in Molecular Biology (IMMUNOLOGY) by UAM. Lecturer 1 Sexenio investigación (six year quality research profesor) Immune evasión mechanism and pathogens inducing cancer and autoimmunity Lecturer(s) CONTACT Name: Estela Paz Artal Phone (ext): estela.pazartal@ceu.es Office: 118, C Lecturer(s) Name: CONTACT Carmen del Águila de la Puente 2

3 Phone (ext): Office: 101, A Lecturer(s) CONTACT Name: Ángela Magnet Dávila Phone (ext): 91372(4721)-91372(4784) Angela.magnetdavila@ceu.es Office: 219,C 2.- TUTORIALS: For any queries students can contact lecturers by , phone or visiting their office during the teacher s tutorial times published on the students Virtual Campus. 3. COURSE DESCRIPTION This course represents the integration of concepts of basic and clinical laboratory immunology allowing to the student of biotechnology know the molecular and cellular mechanism of the immune system and the different methodologies to diagnose different diseases and processes associated with the immune system. The aim of this course consist in conveying an accurate understanding of the basics of the immune system, emphasizing the interconnections between innate and acquired immunity and its impact on health and disease. Knowledge of immunology is required for the understanding and management of the vast majority of known diseases, both for understanding their origin as diagnostic tools. 1.- COMPETENCIES 4. COMPETENCIES Code CB1 CB2 CG1 CG11 Basic and General Competencies Students have been shown to posses and understand knowledge in an area of study that is based on general secondary education at a level that although it is supported by advanced textbooks, includes some issues of the forefont of knowledge of that field of study Students apply their knowledge to their work or vocation in a professional manner, and have competencies that can be demonstrated through devising and defending arguments or solving problems within their field of study. Manage information (capacity for analysis and synthesis) Warrant quality. 3

4 Code CE4 CE6 CE7 Specific Competencies Describe and evaluate cell metabolism and discern the different molecular and chemical mechanisms responsible for a biological process, having acquired an integrated vision of its regulation. Lead and work in a laboratory with biological and / or chemical materials, according to the highest quality standards in force, in each case applying biosafety standards and corresponding waste disposal Manage instrumental resources and apply the methods and the most relevant and innovative techniques in biotechnological applications (analytical, biochemical, molecular, immunochemical and "omics"), including validation testing, data collection and data management and analysis and critical evaluation of them 2.- LEARNING OUTCOMES: Code RA4 RA6 RA7 RB1 RB2 Learning outcomes The student describes and evaluates cell metabolism and discerns the different molecular and chemical mechanisms responsible for a biological process, having acquired an integrated vision of its regulation. The student is able to lead and work in a laboratory with biological and / or chemical materials, according to the highest quality standards in force, in each case applying biosafety standards and corresponding waste disposal The student manages instrumental resources and applies the methods and the most relevant and innovative techniques in biotechnological applications (analytical, biochemical, molecular, immunochemical and "omics"), including validation testing, data collection and data management and analysis and critical evaluation of them Owns and understands knowledge in an area of study from general secondary education, and generally, this knowledge is found at a level that is supported by advanced textbooks, and includes aspects that involving knowledge of the forefront of their study field. The student applies its knowledge to his work or vocation in a professional manner and has the skills typically demonstrated through devising and defending arguments and solving problems within their field of study 4

5 5. LEARNING ACTIVITIES 1.- DISTRIBUTION OF STUDENTS` ASSIGNMENT: Total hours of the course 75 Code Name On-campus hours AF1 Lecture 2 AF2 Seminar 20 AF3 Workshop 2 AF4 Practice 12 AF5 Other 3 TOTAL Presence Hours 39 Code Name Not oncampus hours AF6 Self student work DESCRIPTION OF LEARNING ACTIVITIES: Activity AF1 Lecture AF2 Seminar AF3 Workshop AF4 Practice AF5 Other Definition Learning activity oriented preferably to the competence of acquisition of knowledge (competence 1 MECES) and representative of more theoretical subjects. This activity gives priority to the transmission of knowledge by the professor, with the previous preparation or later study from the student. Learning activity which highlights the participation of the student in the reasoned interpretation of the contents and the sources of the area of study. It is oriented preferably to the competence of the application of knowledge (competence 2 MECES), and also to the ability of gathering, interpreting, and judging information and relevant data (competence 3 MECES). It is representative of mixed profile activities or subjects; theories and practices. Learning activity oriented preferably to the competence of the acquisition of abilities related to transmission of knowledge (competence 4 MECES) and representative of more methodological subjects. It prepares the student for written-oral communication and transmission of knowledge. Learning activity oriented preferably to the competence of application of knowledge (competence 2 MECES) and representative of subjects or practical activities (labs, radio studies, TV studies and/or any other proper space). Evaluation activities 5

6 AF6 Self Student Work Learning activity where the student develops his or her study in an autonomous way working with formative materials. 6. ASSESMENT OF LEARNING 1.- CLASS ATTENDANCE: In order to be eligible for examination by continuous assessment students must attend at least 75% of scheduled class time (attendance sheets will be used). As students may be absent 25% of the classes, no attenuating circumstances will be accepted for absences. 100% attendance at practical classes is required. 2.- ASSESMENT SYSTEM AND CRITERIA: ORDINARY EXAMINATION (continuous assessment) 1 Code Name E1 E1 Written essay-type exam or multiple choice exam (MIDTERM EXAM) Written essay-type exam or multiple choice exam (ORDINARY EXAM) Minimum mark 2 Percentage /70 E22 On site evaluation of theorical work 5 2 E21 Practical Workbooks 5 5 E22 On site evaluation of practical work 5 3 E1 Written essay-type exam (Practical Evaluation) 5 10 E4 Individual activities and oral presentations 5 5 E1 Multiple choice exam (in seminar hours) 5 5 RE-TAKE EXAM/EXTRAORDINARY EXAMINATION Code Name Minimum mark 2 Percentage E1 Written essay-type exam or multiple choice exam 5 70 E21 Practical Workbooks 5 7,5 E22 On site evaluation of practical work 5 7,5 E1 Written essay-type exam (Practical Evaluation) If for justified and extraordinary circumstances, the student cannot attend to the on-campus classes, this must be communicated in written form to the teacher, before the first partial exam. In such case, the percentages previously established for the continuous assessment will be not applicable. The student will be evaluated in the ordinary call about the whole subject, and the final mark in this exam will be 70%. 2 Minimum mark for applying these percentages 6

7 Students who do not make the final exam in ordinary or extraordinary call will be graded always with "No Submission" even if they have not performed any academic activity corresponding to continuous assessment. To approve the practical lessons is mandatory that the grade obtained is greater than or equal to five out of ten 3.- DESCRIPTION OF ASSESSMENT CRITERIA: Assesment criteria Written essay-type exam or multiple choice exam (MIDTERM EXAM) Written essay-type exam or multiple choice exam (ORDINARY EXAMINATION) On site evaluation of theoretical work Definition There will be a partial exam, if possible, out of classes schedule in order to not to reduce the number of classes. In order to pass the exam it will be necessary to have 5 as average grade (multiple choice exam: 70% correct responses). Grade equal or higher than 4.5 will be upgraded if practices grade is 9 or higher and complementary projects have been done. Students with lower grades will need to take the final global, ordinary exam. Percentages for each evaluable item are described above. Ordinary exam: Students having passed the partial exam will take an exam which will exclusively include the second part of the Immunology course. Grading will be as above. Students performing both parts exam need at least 70% correct responses per part to pass Class attendance (signature sheets) and participation. On site evaluation of practical work/ Practical Workbooks/ Written essay-type exam (Practical Evaluation) Individual activities and oral presentations Multiple choice exam (in seminar hours) Written essay-type exam or multiple choice exam (EXTRAORDINARY EXAMINATION) A notebook with the results and interpretation of this on the last day of practice, will be given. The notes both the notebook and the exam must be 5 out of 10. The percentages to be applied to each evaluable parameter are described in Section 2 of this guide. The evaluation of practices should be 5 out of 10 to pass the course in the case of suspending the practice, a review of the same will be done the same day of the regular review of the subject Some issues (on Immunology Diagnostic Techniques) will be proposed. The student will deliver a report and make an oral presentation Scrutinies in the class schedule (will be held from 1 to 2, throughout the course). Short tests to assess the progression of the acquisition of knowledge of students. Students who do not pass the subject in the ordinary call, be submitted to final exam resit, covering all matter contained in the subject. To overcome this, it is also necessary to reach a minimum average grade of 5 (multiple-choice test, 70% correct answers) 7

8 7. COURSE PROGRAMME 1.- COURSE PROGRAMME: THEORETICAL: LESSON 1: INTRODUCTION TO IMMUNOLOGY: definition, natural and acquired immune system, primary and secondary responses, humoral and cellular responses, phylogeny of the immune system. LESSON 2: ORGANS AND CELLS OF THE IMMUNE SYSTEM: primary and secondary organs, innate immune system cells, acquired immune system cells. Hematopoiesis LESSON 3: IMMUNOGLOBULINS: Structure, classes and subclasses, biological functions. Generation of B lymphocytes repertoire. LESSON 4: MONOCLONAL ANTIBODIES: Definition and production. Diagnostic monoclonal antibodies. Therapeutic monoclonal antibodies. Chimeric and humanized antibodies.lecture LESSON 5: COMPLEMENT SYSTEM: definition, main function, classical and alternative pathways, membrane attack complex, anaphylatoxins, other complement functions. LESSON 6: HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX: HLA. HLA-I and HLA-II molecules, structure, function, genetic organization, distribution, polymorphism and heredity. LESSON 7: ANTIGEN PROCESSING AND PRESENTATION: Endocytic and cytosolic pathways for antigen processing and presentation to helper and cytotoxic T lymphocytes. Pharmacological modulation of lymphocyte activation. LESSON 8: T LYMPHOCYTES ACTIVATION AND FUNCTION: T lymphocytes subsets: helper, cytotoxic, regulatory. T cell receptor, TcRαβ and TcRγδ T lymphocytes, generation of diversity. Immune synapse. Function of cytotoxic T lymphocytes. LESSON 9: CYTOKINES: Definition and general characteristics. Functions and mechanisms. Interleukines. Chemokines and lymphocyte circulation. Other cytokines. LESSON 10: ADHESION MOLECULES AND LIGANDS. Definition and function. Types of adhesion molecules. Migration and extravasation. Lymphocyte homing. LESSON 11: THE IMMUNE SYSTEM IN ACTION: Infections. Th1, Th2, Th17 and Treg responses. Immunotherapy: adyuvants, modulators and immunossuppresors. LESSON 12: TOLERANCE: Central mechanisms: thymic and bone marrow clonal deletion. Peripheral mechanisms: clonal anergy and active suppression. Oral tolerance, clinical applications. Maternal-fetal tolerance. Immune privilege sites. LESSON 13: PRIMARY IMMUNODEFICIENCIES: Antibodies immunodeficiencies, cellular and combined immunodeficiencies. Complement deficiencies. Phagocytosis deficiencies. LESSON 14: SECONDARY IMMUNODEFICIENCIES: AIDS. Iatrogenic immunodeficiencies. Other secondary immunodeficiencies. LESSON 15: HYPERSENSITIVITY: Type I: Allergy. Type II: drugs hypersensibility. Type III: serum sickness and farmer s lung. Typo IV: Contact dermatitis. LECTURE LESSON 16: AUTOIMMUNE DISEASES: Concept of autoimmunity. Pathogenenesis theories. Major systemic autoimmune diseases. Major organ-specific autoimmune diseases. 8

9 LESSON 17: TRANSPLANTATION IMMUNOLOGY: Transplantation types: syngeneic, allogeneic, xenogeneic. Allogeneic response. Organ rejection immunobiology. Hyperacute, acute and chronic rejection. Transplantation and immunosuppression. Bone marrow transplantation. Graft versus host disease. LESSON 18: TUMOR IMMUNOLOGY: Anti-tumor immunity. Tumor antigens. Immune surveillance. Anti-tumoral immune therapy strategies. PRACTICAL WORK PROGRAMME: 1. Measurement of serum immunoglobulins. 2. Identification and tritation of autoantibodies by indirect immunofluorescence (IIF) on cells and tissues slides. 3. Detection of autoantibodies by enzyme linked immune sorbent assay (ELISA). 4. Detection of autoantibodies by immunoblot 8. RECOMMENDED READING 1.- ESSENTIAL BIBLIOGRAPHY: Roitt I. (2014). Fundamentos de Inmunología.12ª Ed. Editorial Médica Panamericana. Abbas Abul K. (2015) Inmunología Celular y Molecular. 8ª Ed. Editorial Mc GrawHill/Interamericana. Sompayrac (2016) How the immune System Works 5º Ed. Wiley-Blackwell. 2.- ADDITIONAL BIBLIOGRAPHY: Playfair (2012).Immunology at a Glance, 10th Edition. Wiley-Blackwell Janeway (2015). Inmunobiología. Editorial Masson-Elsevier. Barcelona. J.R.Regueiro. (2010). Inmunología. Biología y patología del sistema inmune. 4ª edición.editorial Médica Panamericana 4.- WEB RESOURCES : Web Sociedad Española de Inmunología SEI : Web European Federation of Inmunological Societies: REGULATIONS 9. ATTITUDE IN THE CLASSROOM Any irregular act of academic integrity (no reference to cited sources, plagiarism of work or inappropriate use of prohibited information during examinations) or signing the attendance sheet for fellow students not present in class will result in the student not being eligible for continuous assessment and possibly being penalized according to the University regulations. 9