A.ABDUL KADER JAILANI

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1 A.ABDUL KADER JAILANI Ph.D. in Eco-Biotechnology, Lab 48, Division of Plant Pathology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, India Mobile: OBJECTIVE To exert my technical and procedural skills through a qualifying position in the field of research in a reputed organization. Professional Snapshot Good hands-on experience in molecular biology, plant genetic engineering, plant virology and microbial biotechnology. Expertise in cloning, infectious clone development based on in vitro transcription and agro, construction of plant viral vector, transient gene expression, protein purification, epitope profiling and overlapping extension PCR for larger RNA virus cdna infectious clone development. Experience in functional genomics, viral vector construction, molecular and serological diagnostics of plant viruses. Academic credentials: Ph.D , Eco-Biotechnology, thesis submitted Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli. Supervisor: Dr. T.Sivasudha, Co-supervisor: Dr. Bikash Mandal 80.0% Coursework Post M.Sc., Advanced , Plant Genetic Engineering, Diploma Sponsored By (Dept of Biotech, India), 67.0% Madurai Kamaraj University, Madurai, Tamilnadu, India Masters Studies , BioTechnology, 66.7% Affiliated to Madurai Kamaraj University, Madurai, Tamilnadu, India Under graduate , BioTechnology, Studies Affiliated to Madurai Kamaraj 53.0% University, Madurai, Tamilnadu, India

2 RESEARCH EXPERIENCE: 1. Ph.D. Thesis Submitted ( ): (Lab: Dr. Bikash Mandal, IARI, New Delhi) Thesis entitled Development of plant viral vectors for expression of heterologous protein in plant system : Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi-12. The full-length CGMMV (6.4 kb) genome was cloned and developed as an infectious clone based on two methodology, in vitro transcription and binary vector construction. The full-length infectious clone was utilized as a gene expression vector to clone GFP and PRSV light chain antibody gene at the end of CGMMV CP terminal and successfully expressed in the plant system. Expression vector of CGMMV was constructed by deleting partial gene sequences of coat protein. Validation of construct was done with GFP and PRSV light chain antibody gene. Both genes were highly expressed compared with the full-length expression vector and proved by real-time PCR and this construct has virus free protein expression in the plant. The purification of PRSV light chain antibody protein was done from the recombinant CGMMV full-length and CP partial deleted vector infected tissue separately and the recombinant proteins can thus be used as antibodies for detection of PRSV in the virus-infected tissue by ELISA and western blot. 2. Currently working as SRF in the project entitled Development of genetically engineered vaccines against economically important poultry viral diseases associated with Dr. Bikash Mandal, Principal Scientist, IARI, New Delhi. This project mainly focuses on application of RNA plant viruses as expression vector in the production of plant-based pharmaceutical proteins without genetic modification of plant. In this work, I have developed full-length infectious CGMMV based expression vector for gene expression. Vaccines were developed against Newcastle disease virus (NDV) Hemagglutinin- Neuraminidase (HN) and Bursal disease virus (IBDV) VP1 and successfully expressed in plant system. The Indian isolate of PVY (9.7 kb) was successfully amplified by overlapping extension PCR and developed as an infectious clone. The clone will be use as an expression vector for future purpose. DNA virus based CYVMV (2.7 kb) genome was utilized as replicon based gene expression vector, confirmed by GFP. 3. Positioned as SRF in ICAR funded a project entitled Genomics & Molecular Breeding of Linseeds / Flax from 10/11/10 to 31/03/2012, associated with Dr. Anand Kumar, NRC on Plant Biotechnology, New Delhi.

3 Differential expressed (DE) genes were found in the subtraction hybridization from the drought stress linseed, DE genes were cloned and sequenced. The DE genes were reconfirmed by realtime PCR in the drought stress filed sample The bri1 gene has an essential role in BR-regulated plant growth and development. The fulllength bri1 gene was isolated from the linseed, mustard and cucumber by genome walking and rapid amplification cdna ends. The full-length bri1 gene was cloned in binary vector and was successfully over expressed into bri1 mutated dwarf arabidopsis and rescues a weak bri1 mutant. The CKX3 and CKX5 genes were isolated from the linseed and mustard plant through genome walking and RACE methodology. Both full-length genes were clone in bacterial expression vector and were over expressed recombinant proteins were purified from the bacteria. The purified protein was exhibits the CKX activity compared to control total plant protein. 4. Positioned as RA in DBT funded project entitled Referral center for molecular diagnosis of transgenic planting materials from 04/11/09 to 22/09/10, associated with Dr. Gurinder Jit Randhawa, National Bureau of Plant Genetic Resources, New Delhi. This project mainly focused on detection of GMO crop based on high throughput method of Real-time, multiplex PCR, qualitative and quantitative analysis of transgenic plants on field levels. This project helped in identification of GMO contamination in the field levels. Apart from that low-cost multiplex PCR-based detection kits were developed and commercialized for GMO crops detection. Dissertations: Post M.Sc., Diploma Title: Host Range Studies on the Soybean Isolate of Mungbean yellow mosaic india virus from Madhya Pradesh. Supervisor: Prof. R. USHA, Madurai Kamaraj University, Tamil Nadu, India This work targeted at understanding the host range of the soybean isolate of Mungbean yellow mosaic india virus (MYMIV) from Madhya Pradesh. The plants tested were cotton, chili, bhindi besides the natural host, soybean. The result of the study is useful for selecting plant species that can be explored by using VIGS vectors based on MYMIV. Post graduation Title: Molecular Identification and Bacterial accumulation of rare earth elements of Manava lakurichi and Chavara.

4 Supervisor: Dr. B. ANANDKUMAR, Lecturer, Sourashtra College, Madurai, Tamil Nadu, India. Publications: 1. A. Abdul Kader Jailani, Vikas Solanki, Anirban Roy, T. Sivasudha, Bikash Mandal (2017). A CGMMV genome-replicon vector with partial sequences of coat protein gene efficiently expresses GFP in Nicotiana benthamiana. Virus Research, 233, IF A. Abdul Kader Jailani, S. Shilpi and Bikash Mandal (2017). Rapid demonstration of infectivity of a hybrid strain of potato virus Y occurring in India through overlapping extension PCR. Physiological and Molecular Plant Pathology, 98, IF Nagamani Sandra, A. Abdul Kader Jailani, Rakesh Kumar Jain, Bikash Mandal (2017). Genome characterization, infectivity assays of in vitro and in vivo infectious transcripts of Soybean yellow mottle mosaic virus from India reveals a novel short mild genotype, Virus Research, 232, IF Sesha Kiran Kollipara, Vikas Solanki, A. Abdul Kader Jailani and Bikash Mandal. Expression of coat protein gene of cucumber green mottle mosaic virus in E. coli and diagnosis of the virus using antiserum to the recombinant coat protein (Accepted in Journal of General Plant Pathology) IF A. Abdul Kader Jailani, Alok Kumar, Bikash Mandal, T.Sivasudha and Anirban Roy (2016). Agroinfection of tobacco by Croton yellow vein mosaic virus and desiging of a replicon vector for expression of foreign gene in plant, Virus Disease 27 (3): IF Prasanta K Dash, Yongguo Cao, Abdul K Jailani, Payal Gupta, prakash venglat, Daoquan Xiang, Rhitu Rai, Rinku Sharma, Nepolean Thirunavukkarasu, Mallik Abdin, D Yadava, Nagendra Singh, Jas Singh, Gopalan Selvaraj, Mike Deyholos, Polumetla Ananda Kumar, Raju Datla ( 2014). Genome-wide analysis of drought induced gene expression changes in flax (Linum usitatissimum), GM Crops & Food: Biotechnology in Agriculture and the Food Chain 5:2, IF Kumar A, A. Abdul Kader Jailani, Anirban Roy and Bikash Mandal. The occurrence, biology and genomic properties of tobamoviruses infecting crop plants in India. (Communicated in Virus disease) 8. A. Abdul Kader Jailani, T. Sivasudha, Bikash Mandal. Papaya ring spot virus light chain antibody expression based on the plant viral vector based system for the use of PRSV diagnosis. (Manuscript under preparation) 9. A. Katiyar, A.Abdul Kader Jailani, A. Kumar and B. Mandal* Complete genome sequence, host range and phylogenetic relationships of an Indian isolate of Potato virus M and diversity analysis of potato virus M populations infecting potato in India. (Manuscript under preparation) Conferences: 1. Daliyamol, A. Abdul Kader Jailani, M krishnareddy, K Vemana and Bikash Mandal (2016). In vitro expression and purification of 2b protein gene of Tobacco streak virus (TSV-GN) from groundnut and development of immunodiagnostics for its detection, Virocon, International conference, Global perspectives in virus disease management, at IIHR, Bengaluru, Dec 8 th -10 th,

5 Page no Daliyamol, A. Abdul Kader Jailani, Anirban Roy and Bikash Mandal (2016). Investigation on role of Tobacco streak virus ORF 2b in virus-host interaction, Virocon, International conference, Global perspectives in virus disease management, at IIHR, Bengaluru, Dec 8 th -10 th, Page no Nagmani Sandra, A. Abdul Kader Jailani, Rakesh Kumar Jain, Bikash Mandal (2016). Genome characterization and development of infectious clone of a strain of a distinct Soybean yellow mosaic mottle virus, 6 th International conference, plant, pathogens and people, at NAS, New Delhi, Feb 23 th -27 th, Page no A. Abdul Kader Jailani, Vikas Solanki and Bikash Mandal (2015). Papaya ring spot virus light chain antibody expression based on the plant viral vector based system for the use of PRSV diagnosis, Virocon, Transboundary viral diseases under one healthy: perspectives & challenges, NEIGRIHMS, Shillong, Oct 8 th -10 th, Page no Daliyamol, A. Abdul Kader Jailani, K Vemana, Anirban Roy and Bikash Mandal (2015). Characterization of complete genome of Tobacco streak virus causing peanut stem necrosis disease in India, Virocon, Transboundary viral diseases under one healthy: perspectives & challenges, at NEIGRIHMS, Shillong, Oct 8 th -10 th, Page no A. Abdul Kader Jailani, Vikas Solanki and Bikash Mandal (2014). A strategy for overcoming clone-instability in E. coli while developing infectious clone of Potato virus Y, Virocon, XXIII National conference on Recent trends in virology research in the omics era, at Coimbatore, TamilNadu, Dec 18 th -20 th, page no A. Abdul Kader Jailani, Bikash Mandal and Anirban Roy (2014). A Croton yellow vein mosaic virus based replicon vector for expressing foreign gene in plant, Virocon, XXIII National conference on Recent trends in virology research in the omics era, at Coimbatore, TamilNadu, Dec 18 th -20 th, page no A. Abdul Kader Jailani, Alok kumar, Anirban Roy, Bikash Mandal (2013). Expression of foreign gene in plant using cucumber green mottle mosaic virus genome with or without coat protein gene, Virocon, Asia pacific congress of virology, at Amity University, Noida, Dec 17 th -20 th, page no Prasanta K Dash, Abdul K Jailani, Payal Gupta, Raju Datla,Michael K. Deyholos, Polumetla Ananda Kumar (2013). Decoding Flax Genome for Structural Genomics and Functional Insights into Yield Genes, Plant & Animal Genome XXI, Jan th, San Digeo, CA Gurinder Jit Randhawa, A. Abdul Kader Jailani, Rashmi Chhabra, Sunil Archak, Ruchi Sharma, Nutan Singh and Priti Sharma (2009). Real-Time Multiplex PCR Method for Qualitative and Quantitative Detection of GM Planting Materials for Safe Movement, In Souvenir and Abstracts, National Symposium on Recent Global Developments in the Management of Plant Genetic Resources, at NBPGR, New Delhi, Dec th, Page no-220.

6 Awards and Appreciation: 1. Got Young Scientist award and cash price in 2015 on Papaya ring spot virus light chain antibody expression based on the plant viral vector based system for the use of PRSV diagnosis, Virocon, Transboundary viral diseases under one healthy: perspectives & challenges, NEIGRIHMS, Shillong, Oct 8 th -10 th, Page no Got Third prize in poster presentation in 2014 A Croton yellow vein mosaic virus based replicon vector for expressing foreign gene in plant, Virocon, XXIII National conference on Recent trends in virology research in the omics era, at Coimbatore, TamilNadu, Dec 18 th -20 th, page no Awarded Rs 5,000/- ( For 1 year ) From Department of Biotechnology, Government of India, for Qualifying the all India entrance Examination for Post M.Sc., Advanced Diploma in Plant Genetic Engineering conducted by Madurai Kamaraj University. June Certificate of Appreciation from National Bureau of Plant Genetic Resources, for participation as laboratory resource person in the ICAR winter school on Molecular markers for efficient management and enhancing utilization of plant genetic resources, January 05-16, Workshop Attended: 1. UKIERI sponsored Indo-UK joint workshop on Plant Molecular Farming II, at Bharathiyar University, Coimbatore, Tamil Nadu, India, 13 th -14 th, March Workshop on Molecular Modeling, at Thiagarajar College, Madurai, Tamil Nadu, India, 27 th -28 th February Training Attended: 1. One week ( to ) hands-on training on Electron Microscope at Cancer Institute (WIA), Chennai, Tamil Nadu, India. GenBank Accessions: 1. Linum usitatissimum brassinosteroid insensitive 1 mrna, GenBank: KM Cucumis sativus brassinosteroid insensitive 1 protein mrna, GenBank: KM Brassica juncea brassinosteroid insensitive 1 protein mrna, GenBank: KM Brassica juncea cytokinin oxidase 3 mrna, GenBank: KM Brassica juncea cytokinin oxidase 7 mrna, GenBank: KM098052

7 Technical Strength: Molecular Biology & Biochemistry Isolation of DNA, RNA & Proteins from different compartments of the cell, protein purification, protein extraction from SDS-PAGE gel, Cloning, Polymerase Chain Reaction, Multiplex PCR, Realtime-PCR, DNA & cdna library construction, genome walker, ssh cdna library construction, DNA & RNA viral vector construction, rapid amplification cdna ends, DNA labelling, Southern & Northern Hybridization (Radioactive and Non-radioactive), SDS-PAGE, Western Blotting, 2D Electrophoresis, TLC, Identification of Sugars & Amino Acids, Protein Estimation. Microbiology Pure culture Techniques & Storage, Microbial Growth Enumeration, Biochemical Tests of Bacteria, Isolation, Enumeration & Characterization of Bacteria & Fungi from different sources, Staining (Simple, Differential, Acid Fast Staining). Plant Tissue Culture Techniques Callus induction (shoot, root, node, axial bud), direct shoot and root induction, Agroinoculation, Agroinfiltration, Vacuum infiltration, Arabidopsis thaliana floral dip method, Agro drench Seed infection, GUS Staining, Acclimatization. Bioinformatics Gene Annotation, Epitope profiling, Primer designing, phylogenetic tree construct, sequence blast, sequence alignment, fully handling bio edit software, database searching, submitting and retrieving of sequences to and from the database. INSTRUMENTS HANDLED: Real Time PCR, Confocal Microscope, ELISA reader, blotting units, PCR, Ultracentrifugation, Lyophilizer, Gel Documentation unit, Sonicator, Nano-drop. Personal Details Father s Name : N.Amirudden Mother s Name : A.Fathima Jan Date of Birth : Age : 31 Gender : Male Permanent Address : B798, 3 rd Floor Budh Nagar, Inderpuri, New Delhi, India Languages Known : Tamil, English, & Hindi

8 Academic References Dr. Bikash Mandal Dr. V. G. Malathi Principal scientist, Adjunct Faculty, Advanced centre for plant virology, Department of Plant Pathology Division of Plant Pathology, Tamilnadu Agricultural University IARI, New Delhi-12. India. Coimbatore India. Mobile: Mobile: Dr. T. Sivasudha, Assistant Professor, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli , Tamil Nadu, INDIA Mobile: DECLARATION I hereby declare that the above information is authentic to the best of my knowledge. A.ABDUL KADER JAILANI