BRAZOSPORT COLLEGE LAKE JACKSON, TX SYLLABUS BIOLOGY 2320 MICROBIOLOGY

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1 BRAZOSPORT COLLEGE LAKE JACKSON, TX SYLLABUS BIOLOGY 2320 MICROBIOLOGY Course Description: Microbiology studies the fundamentals of the biology of microorganisms, including control, infection, host resistance and pathogenicity. It encompasses a survey of bacteria, fungi, protozoa, viruses, and parasites. We will emphasize microbe-human interactions such as disease and the immune response. Prerequisites: BIOL 2401 and chemistry. Chemistry prerequisite may be satisfied by CHEM 1405, CHEM 1411, or one year of high school chemistry. Chemistry prerequisite must have been completed within the past five years. College Level Skills Required: Reading, Writing, Critical Thinking, Mathematics Textbook: Foundations in Microbiology. Talaro, 6 th ed., Mc-Graw-Hill publisher. (Required) Lab Manual: Microbiology Lab: Theory and Applications. Leboffe, 2 nd ed. (Required) Lab Atlas: Photographic Atlas for the Microbiology Laboratory, Leboffe and Pierce, 3 rd ed. (Recommended) Jerry James Cliff O Neal Diane Rouse Mickey Dufilho Kerry Walling Daryl Beatty Jeffrey Detrick Ken Tasa August 2009

2 I. GENERAL OBJECTIVES This course is designed to provide an introduction to Microbiology for those pursuing degrees in health related professions. It is a required course for all students entering health related fields. The course will cover the basic concepts of the infectious nature of microbes balanced with their importance in the functioning of the earth. The course will include the pathology of bacteriology, fungi, protozoan parasites, parasitic worms and viruses. Emphasis is placed on of real life circumstances to teach basic concepts. Current events relating to microbiology are incorporated into the lecture materials. This course will cover related topics including techniques to control and treat infectious agents including physical, chemical and antimicrobial agents. Human host s defenses, including immunology and immunization, are included. The laboratory exercises are designed to give hands on experience in techniques used to observe culture and identify microorganisms using safe and aseptic techniques. Students will learn to use a light microscope to study microbial morphology, various culture medias to isolate and grow microbes, and standard staining techniques. CORE CURRICULUM OBJECTIVES AND ASSESSMENTS As part of the Brazosport College Core Curriculum, this course provides students the opportunity to achieve the following core curriculum objectives: 1. Critical Thinking: Including innovation, creative thinking, inquiry and analysis, evaluation, and synthesis of information 2. Communication Skills: Including effective development, interpretation, and expression of ideas through written, oral, and visual communication. 3. Empirical and Quantitative Skills: Including the manipulation and analysis of numerical data or observable facts resulting in informed conclusions. 4. Teamwork: Including the ability to consider different points of view and to work effectively with others to support a shared purpose or goal. Objectives will be assessed according to the Brazosport College Core Assessment Plan through the sampling and evaluation of student work. II. LEARNING OUTCOMES 1. The student will demonstrate the tools and techniques of microbiology including the methods for culturing microorganisms, the various types of media, staining techniques and the use of the light microscope. Bacterial project 2. The student will be able to identify the structures of procaryotic cell and the structural and functional differences between the gram-positive cell wall, the gram-negative cell wall and other types of cell walls. Test 1

3 3. The student will be able to identify the external and internal structures of eucaryotic cells. The student will be able to classify and identify pathogenic representatives of the Kingdom Mycetae (Fungi), the Kingdom Protista (Algae and Protozoa), and the Kingdom Animalia (Helminths). Test 2 4. Students will be able to describe viral particle structure, the classification system of viruses and the modes of viral multiplication in animal cells and bacteria along with approaches to identify and treat viral infections. Students will demonstrate knowledge of nonviral infectious particles. Students will be able to describe the structure of DNA, the replication of DNA, processes of transcription, translation and regulation of protein. Test 2 5. Students will be able to describe the conditions and requirements that are necessary for microbial growth and the various types of microbial associations, including symbiotic relationships, synergism and antagonism. Test 2 6. Students will demonstrate knowledge of the terminology and modes of action for select physical agents, chemical agents, antimicrobial drugs and mechanical removal methods for controlling microorganisms. Test 3 7. Students will be able to recognize the major factors involved in the development of an infection, the differences between disease causing organisms and normal resident flora. Test 3 8. Students will be able to discuss the development and components of adaptive immunity and will be able to recognize, describe and differentiate dysfunctions in the immune system. Test 4 9. Students will research a current topic in Microbiology and make a 10 minute PowerPoint presentation in class. Presentation 10. Students will develop writing skills through writing reports on laboratory experiments. Laboratory reports III. LECTURE AND LAB OUTLINE Date Lecture Topic Lecture Reading Lab Schedule Lab Exercise Day 1 o Syllabus o Intro to Micro o History of Micro o Scientific method o Koch s Postulates o General Taxonomy Chapter 1 o Introduction to lab o Lab Safety o Data books o Ubiquity of Bacteria Exercise 6 Day 2 o Benefits & Drawbacks Chapter 1 o Observe Exercise 1

4 o of Microbes Current Events o Samples Review Scope use Day 3 o Lab Techniques o Lab Materials, media and stains o Bacterial examples o Handout of culture of medically important bacteria Day 4 o Prokaryotic microbes o Structures, form and function Day 5 o Prokaryotic microbes o Cell wall and Gram Stain o Spores and inclusions Day 6 o Prokaryotic o Medically important ex. o Classification o Handout Bergey s Manual o Turn in Homework (Ch. 1, 3 & 4) 25 pts Day 7 o Eukaryotic microbes o Structures, form and function o Introduction to Fungi o Medically Important fungi o Handouts on fungal distribution and fungal culture Day 8 o Exam #1 (Ch. 1,3,4) o Read Ch. 5 for next time Chapter 3 o Aseptic Technique o Cleaning work area Chapter 4 o Smear Prep o Simple Stain Chapter 4 o Gram Stain o Aseptic Tech- 25pt Chapter 4 o Spore, Capsules, Acid Fast o Aseptic Tech- 25pt Chapter 5 o Pure Cultures o Motility o Results o Stain Quiz 25 pt Exercise 8 Exercises 10,11 Exercise 14 Exercises 13, 15 Exercises 9,17 Day 9 o Eukaryotic microbes ( Medically important Protists and helminths) o Last day to get presentation topic approved Chapter 5 o Fungal Culture Exercise 22 Day 10 o Viruses o Structure & Function Day 11 o Virus Introduction o Viral Components/Classifica tion Chapter 5,6 o Acid Fast Stain o Results Chapter 6 o Bacteriophage Titer Exercise 16 Exercises 23

5 o o o Viral Replication Culturing viruses Current events Day 12 o Microbial nutrition & growth (temp., ph, nutrients, and environmental factors) Chapter 7 o Bacteriophage and Fungal Culture Results Day 13 o Microbial growth continued (ecological associations & techniques to quantify growth) o Turn in homework (Ch. 5-7) 25 pts Chapter 7 1. Testing Antibiotics & Disinfectants Exercises 33,34 Day Microbial Resistance Factors & physical and chemical methods of Control Chapter Results Day Exam #2 (Ch. 5,6,7) 5. Read Chap. 12 Day 16 o Library Work on class Presentation (no class) 6. Library work on Presentation (no class) 7. Turn in notebooks &Lab Midterm (50 pts) Spring Break Day Drugs & Drug Targets ( principles of drug action on microbes) ( Turn in draft of presentation- 25 pts) Chapter Morph. Study of Exercise 36, 41 Day Drugs & Effects on the Host 11. Microbe human interactions (normal flora, portal of entry) Chapter 12, Results Day Infection & Disease 14. Virulence Factors Chapter Cultural Characteristics of Exercise 37 Day 20 Day Infection & Disease 17. How Microbes spread 18. Epidemiology 19. Turn in homework (Ch ) 25 pts 21. Immunity & Host 22. Non specific host defenses (interferon Chapter 13,14 Chapter Results 24. Oxidation/Ferm. Tests of Exercise 38

6 and complement) 23. How infection is fought Day Exam # 3 (Ch ) 26. Read Ch. 15 for Tues 27. Results Day The Lines of Defense st, 2 nd, and 3 rd Lines 30. Specific immunity Chapter Hydrolytic Reactions of Exercise 39 Day Antigens and the immune response 33. Complement & Macs Chapter 15, Results Day Acquiring Specific Immunity 36. T and B cells 37. Immunization Chapter Biochemical Tests of Exercise 40 Day Disorders in Immunity 40. Current Topics 41. Turn in homework (Ch ) 25 pts Chapter Results turn in Descriptive Chart (p. 223) with ID of unknown (100pts) Day Class Presentations 75 pts 44. Turn in notebooks 45. Lab Final (50pts) Day Exam #4 (Ch15-17,9 & 10 parts) 47. Class Presentations 75 pts Day Optional Final Comprehensive Finals Week 49. No Lab IV. GRADING The total course grade will come from two sources- the lecture and the lab, calculated as follows: Overall Grade: Lab 400 pts Exams 400 pts Homework 100 pts Presentation 100 pts 1000 pts possible lab & lecture Laboratory Grade: Lab Midterm/Final 100 pts Notebook (15 labs x 10 pts) 150 pts Demo Skills & Quiz 50 pts s 100 pts Safety (gas lines, etc...) minus pts 400 pts possible - lab

7 V. COURSE POLICIES A. STUDENTS WITH DISABILIITIES BC is committed to providing equal education opportunities to every student. BC offers services for individuals with special needs and capabilities including counseling, tutoring, equipment, and software to assist students with special needs. Please contact Phil Robertson, Special Populations Counselor, for further information. B. WITHDRAWAL POLICY The Brazosport College Biology Department believes attendance in both lecture and lab is critical for the comprehension of material. Therefore, if student absences exceed requirements set by the instructor, he may be withdrawn from the course. (The instructor will provide written guidelines as to these attendance requirements.) C. ACADEMIC HONESTY BC assumes that students eligible to perform on the college level are familiar with the ordinary rules governing proper conduct including academic honesty. The principle of academic honesty is that all work presented by you is yours alone. Academic dishonesty including, but not limited to, cheating, plagiarism, and collusion shall be treated appropriately. Please refer to the BC Student Guide for more information, this is available online at click on the link found on the left side of the homepage.