Erythrocyte Membrane Modified Janus Polymeric

Transcription

1 Supporting Information Erythrocyte Membrane Modified Janus Polymeric Motors for Thrombus Therapy Jingxin Shao, Mona Abdelghani, Guizhi Shen, Shoupeng Cao, David S. Williams, Jan C. M. van Hest* Bio-Organic Chemistry, Eindhoven University of Technology, P.O. Box 513 (STO 3.41), 5600 MB Eindhoven, The Netherlands. *Correspondence to: Contents: Figure S1. Schematic illustration and zeta potential of (Hep/CHI) 5 microparticles. Figure S2. SEM images of naked silica particle templates and (Hep/CHI) 5 microparticles. Figure S3. SEM and CLSM image of isolated erythrocyte membrane, and SDS-PAGE protein analysis. Figure S4. UV spectrum and tracking trajectories. Figure S5. Relative velocity and relative diffusion coefficient. Figure S6. SEM images of JPMs before and after coating with cell membrane in PBS solution. Figure S7. SEM images of JPMs before and after coating with cell membrane as a function of culture solutions (cell culture medium, serum, and blood). Figure S8. Biocompatible assessment of EM-JPMs by MTT assays. Figure S9. Biodegradable measurement. S1

2 Figure S10. CLSM and SEM images of EM-JPMs before and after NIR irradiation. Figure S11. Time-lapsed two photon CLSM images. Video S1. Brownian motion of EM-JPMs without NIR illumination. Video S2. Movement behavior of EM-JPMs upon NIR illumination. Video S3. Off/On controllable motion of EM-JPMs. Video S4. Photothermal ablation of thrombus in the presence of EM-JPMs which were prepared by CHI and Hep. Video S5. Photothermal ablation of floating thrombus. Video S6. Photothermal ablation of thrombus in the presence of EM-JPMs which were prepared by CHI and ALG. Video S7. Thrombus models directly irradiated with NIR laser. Figure S1. (A) Schematic illustration of the fabrication of layer-by-layer (LbL) self-assembly microcapsules by electrostatic interaction. (B) Zeta potential of microparticles during the alternate deposition of Heparin (Hep) and Chitosan (CHI) layers as a function of the number of deposition layers. Each data point is the mean value of three successive measurements; the standard deviations are too small to be visible on the graph. S2

3 Figure S2. SEM images of naked silica particle templates (left) and (Hep/CHI)5 particles (right). Scale bar = 10 µm. Figure S3. (A) SEM images of isolated erythrocyte membrane. Scale bar = 10 µm. (B) CLSM images of erythrocyte membrane which was pre-stained with Alex 488 fluorescence dye. Scale bar = 20 µm. (C) SDS-PAGE protein analysis. S3

4 Figure S4. (A) UV spectrum of EM-JPMs. (B) Tracking trajectories at different NIR laser power. Figure S5. Relative increase in velocity (A) and diffusion coefficient (B) of EM-JPMs compared to bare JPMs as a function of different solutions (PBS, cell culture medium, serum, and blood). S4

5 Figure S6. SEM images of JPMs without removal of templates before and after coating with erythrocyte membrane in PBS solution for 1 h and 6 h, respectively. Figure S7. SEM images of JPMs without removal of templates before and after coating with erythrocyte membrane as a function of culture solutions (cell culture medium, serum, and blood) at 1 h and 6 h, respectively. S5

6 Figure S8. Biocompatibility assessment of EM-JPMs by the MTT assay. NIH/3T3 cells were incubated with EM-JPMs for 24 h. The data were composed of average value and standard deviation. The experiment was performed in quintuplicate. S6

7 Figure S9. Morphology changes of JPMs after incubation in PBS solution at 37 oc for 1 h, 3 h, 6 h, and 24 h, respectively. Scale bar = 3 µm. S7

8 Figure S10. CLSM images of EM-JPMs before (A) and after (B) NIR irradiation. Scale bar = 10 µm. SEM images of EM-JPMs before (C) and after (D) NIR irradiation. Scale bar = 1 µm. S8

9 Figure S11. Time-lapsed two photon CLSM images. (A) Fluorescent thrombus in the presence of EM-JPMs prepared by (CHI/ALG) 5 irradiated by a 760 nm laser. (B) Fluorescent thrombus without EM-JPMs was directly illuminated with laser as control group. (C) Thrombolytic therapy in the presence of (CHI/Hep) 5 capsules without Au coating. Scale bar = 20 µm. S9