For focused group profiling of human nuclear receptors and coregulators genes expression

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1 ExProfile TM Human Nuclear Receptors and Coregulators Related Gene qpcr Array For focused group profiling of human nuclear receptors and coregulators genes expression Cat. No. QG040-A (1 x 96-well plate, Format A) Cat. No. QG040-B (1 x 96-well plate, Format B) Cat. No. QG040-C (1 x 96-well plate, Format C) Cat. No. QG040-D (1 x 96-well plate, Format D) Cat. No. QG040-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human nuclear receptors and coregulators related gene qpcr array profiles the expression of 84 human genes related to nuclear receptors and coregulators. These genes are carefully chosen for their close correlation based on a thorough literature search of peer-reviewed publications, mainly including genes that encode various nuclear receptors, transcription factors and regulators, as well as chromatin modification molecules and other nuclear receptor co-regulators. This array allows researchers to study the related genes to gain understanding of their roles in the functioning and characterization of nuclear receptors and coregulators. QG040 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG040 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ PPARA QG A02 HQP NM_ BRD8 QG A03 HQP NM_ AR QG A04 HQP NM_ HPRT1 QG A05 HQP NM_ NR2C1 QG A06 HQP NM_ VDR QG A07 HQP NM_ TRIP4 QG A08 HQP NM_ THRB QG A09 HQP NM_ THRAP5 QG A10 HQP NM_ THRAP4 QG A11 HQP NM_ THRAP1 QG A12 HQP NM_ THRA QG B01 HQP NM_ RXRB QG B02 HQP NM_ RXRA QG B03 HQP NM_ RBPJ QG B04 HQP NM_ RARG QG B05 HQP NM_ RARB QG B06 HQP NM_ RARA QG B07 HQP NM_ PSMC3 QG B08 HQP NM_ PPARGC1B QG B09 HQP NM_ PPARGC1A QG B10 HQP NM_ PPARG QG B11 HQP NM_ PPARD QG B12 HQP NM_ PPARBP QG C01 HQP NM_ PPARA QG C02 HQP NM_ PCAF QG C03 HQP NM_ NRIP1 QG C04 HQP NM_ NR4A1 QG C05 HQP NM_ NR3C2 QG C06 HQP NM_ NR3C1 QG C07 HQP NM_ NR2F6 QG C08 HQP NM_ NR2F2 QG C09 HQP NM_ NR2C2 QG C10 HQP NM_ NR1I2 QG C11 HQP NM_ NR1H4 QG C12 HQP NM_ NR1H3 QG D01 HQP NM_ NR1H2 QG D02 HQP NM_ NR1D2 QG D03 HQP NM_ NR1D1 QG D04 HQP NM_ NR0B2 3

4 4 Product Data Sheet QG D05 HQP NM_ NOTCH2 QG D06 HQP NM_ NONO QG D07 HQP NM_ NFKB2 QG D08 HQP NM_ NCOR1 QG D09 HQP NM_ TGS1 QG D10 HQP NM_ NCOA4 QG D11 HQP NM_ NCOA2 QG D12 HQP NM_ MTA1 QG E01 HQP NM_ MED4 QG E02 HQP NM_ MED12 QG E03 HQP NM_ HTATIP QG E04 HQP NM_ HDAC7A QG E05 HQP NM_ HDAC6 QG E06 HQP NM_ HDAC5 QG E07 HQP NM_ HDAC4 QG E08 HQP NM_ HDAC3 QG E09 HQP NM_ HDAC2 QG E10 HQP NM_ HDAC1 QG E11 HQP NM_ ESRRG QG E12 HQP NM_ ESRRA QG F01 HQP NM_ ESR2 QG F02 HQP NM_ ESR1 QG F03 HQP NM_ DDX5 QG F04 HQP NM_ CRSP6 QG F05 HQP NM_ CRSP2 QG F06 HQP NM_ CREBBP QG F07 HQP NM_ COPS2 QG F08 HQP NM_ ARNT QG F09 HQP NM_ AR QG F10 HQP NM_ AHR QG F11 HQP NM_ NR0B1 QG F12 HQP NM_ RORA QG G01 HQP NM_ RXRG QG G02 HQP NM_ BRD8 QG G03 HQP NM_ ESRRB QG G04 HQP NM_ HMGA1 QG G05 HQP NM_ HNF4A QG G06 HQP NM_ PSMC5 QG G07 HQP NM_ NCOA1 QG G08 HQP NM_ NCOA3 QG G09 HQP NM_ NR1I3 QG G10 HQP NM_ NR2C1 QG G11 HQP NM_ NR2E3 QG G12 HQP NM_ NR2F1 QG H01 HGDC QG H02 HGDC

5 QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Following terms and conditions apply to use of ExProfile TM Human Nuclear Receptors and Coregulators Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG