Changing the world of laboratory developed testing

Transcription

1 Changing the world of laboratory developed testing Solution overview Explore the solution

2 Experience a convenient and easy workflow FLOW Primary Sample Handling Instrument MagNA Pure 96 Instrument FLOW PCR Setup Instrument Roche qpcr Instrument DISCLAIMER Back to overview

3 Experience a convenient and easy workflow FLOW Primary Sample Handling Instrument Accepts all major sample types Flexibly accepts primary & secondary sample tubes Pipets sample into MagNA Pure 96 Processing Cartridge Spike Samples with total Process Controls Comprehensive process surveillance (clot, bubble and liquid level detection) GMP manufactured by Hamilton Technical specifications MagNA Pure 96 Instrument FLOW PCR Setup Instrument DISCLAIMER Roche qpcr Instrument Back to overview

4 FLOW Primary Sample Handling Instrument Primary Sample Handling Instrument Purpose Primary Sample Handling for the Roche MagNA Pure 96 System Transfer of samples from common tube types into the Roche MagNA Pure 96 Processing Cartridges Type Benchtop instrument Dimensions cm (W D H) Weight Run time Setup time Sample types Supported primary/ secondary tube types Plate capacity Sample volume Liquid handling 163 kg Transfer of 96 samples from sample tubes to plates in approximately 20 minutes depending on sample material Up to 15 minutes to manually place samples, tips, and plates onto loading tray Stage loading, including barcode reading is performed automatically Without pretreatment: Whole blood, serum, plasma, cell suspension (resuspended in PBS), saliva, urine With pretreatment: BAL, stool, swab eluates, CSF, sputum mm, mm, mm, mm Adapters to fit FLOW Tubes (2 ml) Can support up to 256 samples per run 1 Processing FLOW Cartridge carrier for maximum 3 MagNA Pure 96 Processing Cartridges µl, 1,000 µl for 48 samples only One pipetting arm with 4 independently controlled air displacement channels Liquid level detection Capacitive liquid level detection (clld) Min. pipetting volume 50 µl for sample, 20 µl for internal control Barcode scanning System sterility Barcode scanner mounted on the autoload slide for tips and tubes Barcode scanner fixed on the instrument deck for plates UV light Disposables Tips Tip High Vol, 1 ml with Filter Plates 96 well MagNA Pure 96 Processing Cartridges, Note: Maximum volume depends on used sample volume Tubes FLOW Tubes (2 ml)

5 Experience a convenient and easy workflow FLOW Primary Sample Handling Instrument MagNA Pure 96 Instrument Offers high speed nucleic acid isolation for up to 96 samples in less than 1 hour Flexibly accepts more than 10 different sample types within one run Provides for result safety through run surveillance and tracking Consistent sample handling with excellent intra run, inter-run, and lot-to-lot precision GMP manufactured, CE-IVD registered Connect upto three MagNA Pure 96 Systems per line Technical specifications FLOW PCR Setup Instrument DISCLAIMER Roche qpcr Instrument Back to overview

6 MagNA Pure 96 Instrument MagNA Pure 96 Instrument Purpose The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from a wide range of starting materials using magnetic glass particle technology (MGP) Type Benchtop instrument Dimensions cm (W D H) Weight 235 kg Run time Approximately 50 to 60 minutes for 200 μl sample volumes Approximately 80 to 90 minutes for 500 μl sample volumes Racks Drawer-like loading racks for extraction reagents Sample number 1 to 96 reactions per run Plate capacity 1 for MagNA Pure 96 Processing Cartridges 1 for MagNA Pure 96 Output Plates Sample volume Elution volume 50 to 200 µl Liquid handling Liquid pressure detection Barcode scanning System sterility Disposables Tips Plates Reagents System fluid Prefilled, ready-to-use reagents 50 to 500 μl with 96 reactions per run, 1,000 µl for 48 samples only Two robotic arms: 1. Reagent head with four individually controlled fluid channels 2. Process head with a 96-nozzle pipette head (CO-RE tip technology) Liquid pressure sensor Internal and external barcode scanner UV lights MagNA Pure 96 Filter Tips (1,000 µl) MagNA Pure 96 Processing Cartridges MagNA Pure 96 Output Plates MagNA Pure System Fluid Internal Container, for 2 L, or MagNA Pure System Fluid External Container, for 5 L MagNA Pure 96 DNA and Viral NA Small Volume Kit (CE-IVD) MagNA Pure 96 DNA and Viral NA Large Volume Kit (CE-IVD) MagNA Pure 96 Cellular RNA Large Volume Kit (For life science research only. Not for use in diagnostic procedures.)

7 Experience a convenient and easy workflow FLOW Primary Sample Handling Instrument MagNA Pure 96 Instrument FLOW PCR Setup Instrument Data driven pipetting Highly flexible PCR Setup 96 and 384 well plate capability Automated master mix creation GMP manufactured by Hamilton Technical specifications Roche qpcr Instrument DISCLAIMER Back to overview

8 FLOW PCR Setup Instrument PCR Setup Instrument Purpose qpcr reaction setup in 96 or 384 PCR plate formats Type Benchtop instrument Dimensions cm (W D H) Weight 180 kg Setup time Up to 15 minutes Run time Dependent on number of samples, complexity of reaction mix setup, and number of total reactions; minutes for most common applications Plate capacity 5 positions for LightCycler 480 Multiwell PCR Plates 3 positions for the MagNA Pure 96 Output Plates 2 positions for RT reaction plates (requires external incubation) Automated master mix preparation Liquid handling Liquid level detection Minimal pipetting volume Barcode scanning Disposables Tips Plates Tubes For ready-to-use master mixes, detection mixes, or single components Up to 3 components (enzyme, water, additional component) can be combined for master mix reactions Up to 3 components (forward primer, reverse primer, probe) can be combined for reaction mix preparation Supports up to 21 components to create one PCR master mix 8 independent 1 ml pipetting channels Capacitive liquid level detection (clld) 5 μl to empty well, 2 μl to partially filled well in 384-well, and 5 μl to empty well, 5 μl to partially filled well in 96-well Barcode scanner mounted on the autoload slide for tips and tubes Barcode scanner fixed on the instrument deck for plates 1 ml, 300 µl and 50 µl filter tips MagNA Pure 96 Output Plates LightCycler 480 Multiwell Plates 96 wells and 384 wells (Specific plates with 4 barcodes only) FLOW Tubes (2 ml)

9 Experience a convenient and easy workflow FLOW Primary Sample Handling Instrument MagNA Pure 96 Instrument FLOW PCR Setup Instrument Roche qpcr Instrument Speeds through 40 cycles in minutes Well established industry standard with thousands of placements world wide High Performance: Temperature accuracy Sensitivity Connection of up to five 96-well or 384-well Roche qpcr Instruments GMP manufactured DISCLAIMER Technical specifications Back to overview

10 Roche qpcr Instrument Roche qpcr Instrument Purpose qpcr reaction in 96 or 384 PCR plate formats Type Benchtop instrument Dimensions cm (W D H) Weight 55 kg Run time < 40 min. for 40 cycles in 384-well plate format 40 cycles, 2-step protocols: < 40 min. (384) 40 cycles, 2-step protocols: < 60 min. (96) Reactions per run Up to 96 or 384 reactions per run (384 well block available for LightCycler 480 only) Probe/assay format Intercalating dyes and Hydrolysis probes Multiplexing capability 6x Cq uniformity SD < 0.2 Temperature accuracy ± 0.2 C of target temperature FLOW Reaction volumes 7 20 μl (384)/ μl (96) Analysis type Absolute quantification based on 2 nd derivative maximum method Disposables Plates LightCycler 480 Multiwell Plates 96/384 wells (Specific plates with 4 barcodes only)

11 Experience a convenient and easy workflow The FLOW Software seamlessly connects all FLOW components with the Laboratory Information System, or intermediate Middleware solution. Complete integration of all assays and data into a fully traceable database Ensures result safety with minimal manual interventions Statistical Analysis Module for quality control of PCR Control materials Solution overview Unparalleled scalability Assay menu DISCLAIMER Back to overview

12 FLOW Systems Changing the world of laboratory developed testing Open qpcr workflow solution providing Primary Sample Handling Nucleic Acid Extraction PCR Plate Setup qpcr. FLOW Software seamlessly connects the workflow, ensuring full traceability of data and LIS or middleware connectivity. Delivering qualitative and quantitative PCR analysis in both 96 and 384 well configurations.

13 Unparalleled Scalability allows you to react to changing workload demands (1 MagNA Pure 96) Results First 96 samples in less than 4 hours samples in 10 hours Hours Results (2 MagNA Pure 96) First 192 samples in less than 4 hours samples in 10 hours Hours Results (3 MagNA Pure 96) First 276 samples in less than 4 hours samples in 10 hours Hours Due to the unique modular configuration of each FLOW Line additional nucleic acid purification and qpcr systems can be incorporated to increase capacity and provide contingency. Add up to 3 MagNA Pure 96 Nucleic Acid Purification Systems and up to 5 LightCycler 480 / Cobas z480 qpcr Systems

14 Open System, Endless Possibilities The LightCycler 480 Real-Time PCR System used in the is a 6 channel precision amplification and detection platform that can be used to support Development and routine use of Laboratory Developed Tests Pick from an extensive range of individual LightMix Modular Assays* that utilise a universal amplification protocol to create custom pathogen detection panels specific to your needs: Wide menu of Respiratory, Gastrointestinal, Sexually Transmitted and Transplant Related Infectious Diseases Multiplex up to 6 Assays in a single well Perform any combination of LightMix Modular Assays on the same plate in the same run Utilise third party commercial test kits validated for use on the MagNA Pure 96 and LightCycler 480 Systems * LightMix Modular Assays for Research Use Only, are developed and manufactured by TIB MOLBIOL GmbH, Berlin Germany and exclusively distributed by Roche. LIGHTMIX MODULAR is a trademark of TIB MOLBIOL.

15 Disclaimer The is for General Laboratory Use. MAGNA PURE, LIGHTCYCLER and TAQMAN are registered trademarks of Roche LIGHTMIX MODULAR is a trademark of TIB MOLBIOL. LightMix Modular Assays are developed and manufactured by TIB MOLBIOL GmbH, Berlin Germany and exclusively distributed by Roche. Lightmix Modular Assays are for Research Use Only. Roche Diagnostics Limited. All rights reserved.