Western Blot One Stop Solution. brand new experience

Transcription

1 Western Blot One Stop Solution brand new experience

2 5 One day of WB Step 1 Step 2 Sample prep min fast electrophoresis Step 3 eblot fast wet tranfer completed Steps easily complete experiment begin a New Style Step 4 ONE-HOUR detection blocking, incubation, washing and detection Exposure and development Sample prep SDS and LDS sample buffer are commonly used. Merit of LDS sample buffer Strong PH preservability Phenol red shows clearer front end Excluding DDT compatible with both nature and denature page Strong denaturing ability Rarely precipitate under low temp storage More suitable for silver staining 4X LDS Sample Buffer ml M X LDS Sample Buffer 2 ml M X Sample Buffer(SDS buffer) 5 ml MB015 For better result, we recommend to use 4X LDS sample buffer (M00676) SDS-PAGE SurePAGE gels are a major update from ExpressPlus gels. Fast running : minimum 18 mins High resolution: evener sharper bands RT storage: 28 up to half a year Strong flexibility: rarely broken Large volume maximum 80 ul

3 Product list Gel Concentration(%) Seperation Range -180 kda -160 kda kda -160 kda -2 kda -2 kda ExpressPlus SurePAGE well 12 well 15 well well 12 well 15 well M008 M00812 M00815 M00661 M00662 M00663 M0 M012 M01115 M00664 M00665 M00666 M012 M01212 M01215 M00667 M00668 M00669 M816 M81612 M81615 M00658 M00659 M00660 M412 M41212 M41215 M00652 M00653 M00654 M4 M412 M415 M00655 M00656 M00657 Running buffer and protein marker large protein (high MW) use MOPS and low concentration gels small protein (low MW) use MES and high concentration gels M00624 Pre-stained Marker MM1397 Pre-stained Marker M00521 WB Marker kda kda kda Electrophoresis related products Tris-MOPS-SDS Running Buffer Powder MES SDS Running Buffer Powder PAGE-MASTER Protein Standard (for SDS-PAGE) PAGE-MASTER Protein Standard Plus WB-MASTER Protein Standard Broad Multi Color Pre-Stained Protein Standard 1 Box (5/PK) 1 Box (5PK) 0 μl 0 μl 2 µl 2 µl 12 µl M00138 M00677 M00516 MM M00521 M M Coomassie blue staining analysis we recommend -min Protein Gel Staining device (L00657), compared to traditional menthod, it has better sensitivityand uniformity, present you better result. SurePAGE Gel: SurePAGE, 4-12%, 12 wells (Genscript, M00653) Sample: Top cell Stain: estain L1 Protein Staining Device (Genscript, L00657C),9 min s

4 Coomassie blue staining analysis estain TM L1 Protein Staining Device 1 Nint estain TM L1 Protein Staining Kit (international version coming soon) 1 Kit Kit components are stain, destain reagent and filiter paper, for more info please viset our website L00657 L00687R Protein Transfer eblot L1 Fast Wet Transfer System, on the basis of wet tranfer, with ultra-efficiency and simplicity. Simple -one button operation Efficient-Simultaneously tranfer high MW and low MW Safe- non-dangerous format, protect your health Fast-Complete transfer in 9-17 minutes Clean- automatically pumped in and out, extra cooling system Stable-strict QC tests, highly repeatable results Cases IgG (~1 kda, NC membrane, SurePAGE) Tubulin (~ kda, NC membrane, SurePAGE) EGF (~7 kda, NC membrane, SurePAGE) ng 25 ng 12.5 ng 6.25 ng ng μg 25 μg 12.5 μg 6.25 μg μg ng 5 ng 2.5 ng 1.25 ng ng Traditional wet transfer Competitor A Competitor B GenScript s eblot Product list eblot L1-1 unit eblot L1 Transfer sandwich without membrane (with buffers for NC) Complete eblot L1 Transfer sandwich (NC) eblot L1 Transfer sandwich without membrane (with buffers for PVDF) Complete eblot L1 Transfer sandwich (PVDF) 1 Unit 1 Kit ( reations) 1 Kit ( reations) 1 Kit ( reations) 1 Kit ( reations) L00686 L00724 L00725 L00726 L00727

5 Blocking/Antibody Incubation/Development High quality golden standard primary antibody advantages Ultra Sensitivity Ultra Reactivity Ultra Stability control Counts M1 Counts M1 Flow cytometric analysis of HEK293 cells (A) and HEK293 cells transfected with His tagged fusion gene His-FITC A His-FITC B 3 4 (B) using THE His Antibody, mab, Mouse (A00186). Monoclonal antibody product list Tag Ig Type Conjugation Application His GFP cmyc GST HA FLAG NWSHPQFEK V5 Protein C BrdU Rabbit IgG Mouse IgG2a, k Mouse IgG2a Mouse IgG1 Mouse IgG1 Mouse IgG2b, k Mouse IgG2a, k HRP HRP ELISA, FACS, IP, WB, IF FACS, IP, WB, ICC/IF ELISA, IP, WB ELISA, FACS, IP, WB, ICC/IF ELISA, FACS, IP, WB, ICC/IF IP, WB, FCM, ICC/IF ELISA, FACS, IP, WB, IHC A00186 A01704 A00704 A00863 A00865 A00866 A01244 A00187 A01732 A01724 A01774 A016 Loading Control Antibody GenScript offer stable loading control antibodies, convenient for detecing interested protein, revising loading error, making more reliable result for your WB experiment. they can also be uesd as lank control, to detect if tranfer is complete and coloring system works well. Below picture shows sensitivity comparison of Anti-β-actin mab between X kd M Primary Antibody: A. 0.1 μg/ml Mouse Anti-β-actin mab (GenScript, A000702) B. 0.1 μg/ml Mouse Anti-β-actin mab (X Company, A00702) Secondary Antibody: Goat Anti-Mouse IgG (H&L) [HRP] pab (GenScript,1:000,A00160) 293 cell lysate (μg) 4 A B Substrate Kit: Lumisensor TM HRP Substrate Kit (GenScript,L00221V0)

6 Product List Product Name THE TM beta Actin Antibody, mab, Mouse THE TM alpha Tubulin Antibody, mab, Mouse THE TM alpha Tubulin Antibody [HRP], mab, Mouse THE TM alpha Tubulin Antibody [Biotin], mab, Mouse THE TM beta Actin Antibody [Biotin], mab, Mouse Application A A014-0 A01490 A01545 A01546 ONE-HOUR Western Detection System The ONE-HOUR Western TM Detection System is designed to produce high signal with low background for quick and clear western analysis of proteins. GenScript's breakthrough ONE-HOUR Western TM technology simplifies the classical western blot analysis by skipping secondary antibody binding and washing steps. The kits reduce the total western blot analysis time from 4.5 hours down to only one hour. Easy to perform: Quick and simple procedure. High sensitivity: The sensitivity of the ONE-HOUR Western is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and quantity of antibodies used. Highly reproducible results. Less optimization needed than the classical method. Secondary antibody is included. No special labeling required for primary antibody Comparison of the ONE-HOUR Western TM Kit to the classical three-step Western procedure is shown below GenScript ONE-HOUR Western TM Blot Detection Primary Antibody Blocking Secondary Antibody Development HOUR Classical Western Blot Detection 4.5 HOURS Blocking Primary Antibody Secondary Antibody Development Product List Product Name ONE-HOUR Western TM Basic Kit (Mouse) ONE-HOUR Western TM Standard Kit (Mouse) ONE-HOUR Western TM Standard Kit (Rabbit) ONE-HOUR Western TM Basic Kit (Rabbit) ONE-HOUR Western TM Advanced Kit (Rabbit) ONE-HOUR Western TM Advanced Kit (Mouse) L005 L005C-1 L004C-1 L004C L00241 L Centennial Ave., Piscataway, NJ 08854, USA Toll-Free: Fax: All Rights Reserved. All content described by GenScript is copyright of GenScript Corporation unless specifically identified otherwise. This includes all imagery, text and programmatic computer code. PFA180614