Spring 2016 Office Phone: (714)

Transcription

1 Course Syllabus Derek Boyer Biology 210 Spring 2016 Office Phone: (714) Office: Bio Sc. 212A Hrs: T/Th 11:15-1:15pm Course Description: Biol 210 is a 5-unit college transferable course that introduces you to the microbial world a world of viruses, bacteria, molds, yeasts, protozoa, algae, and helminths. Emphasis will be placed on microbial structure, metabolic activities, microbial genetics, hostparasite relationships, host resistance, and the control of microbes. Lab activities will include isolation and cultivation of bacteria, studies of bacterial isolates, understanding bacterial population dynamics, control of microbial growth, mycology, parasitology, and virology. This course is recommended for biological science majors, pre-nursing, pre-medical, pre-dental, prepharmacy, and other life science majors. Required Textbook: Microbiology: An Introduction 12th Ed by Tortora, Funke, and Case Required Lab Manual: Laboratory Theory and Application, 4th Ed. by Leboffe and Pierce Derek Boyer s webpage: has links to supplementary materials and a copy of this course syllabus. Student Learning Outcomes: 1. Demonstrate appropriate laboratory skills and techniques related to the isolation, staining, identification, assessment of metabolism, and control of microorganisms. 2. Evaluate the physical and chemical methods used in the control of microorganisms and apply this understanding to the prevention and control of infectious diseases when given a scenario. 3. Discuss and diagram the role of the immune system in maintaining homeostasis, challenging infections, and fighting cancer. Grading: Your course grade is based on a percent of accumulated points. These points are acquired by examinations, quizzes, lab assignments, and lab reports. The grading scale is: A = % (4) Lecture exams 125 pts. ea. = 500 B = 80-89% (5) Lecture quizzes 20 pts. ea. = 80 (1 dropped) C = 70-79% (1) Diseases assignment 20 pts. = 20 D = 60-69% (2) Lab exams 80 pts. ea. = 160 F = Below 60% (1) Lab final 100 pts. = 100 (5) Lab quizzes 10 pts. ea. = 40 (1 dropped) (3) Lab question sets 20/20/10 = 50 (1) Lab unknown project 30 pts. = 30 Lab participation/professionalism 20 pts. = 20 Total Possible Points = 1000 points

2 Lecture Policy Lecture has a total of 600 points that come from 4 exams, online quizzes and assignments, and 1 diseases assignment. Make-up exams: Only one make-up lecture exam will be allowed during the semester and it must be taken within 1 week of the missed exam. Based on an excused absence you must contact me the day of the exam. Make-ups may be essay exams. Lab Policy Points for lab total 400 and come from 2 midterm exams, a final exam, 5 quizzes (lowest quiz/missed quiz will be dropped), question sets, an unknown project, and participation/professionalism. Late lab assignments may not be accepted or will be given a penalty depending on the time frame of the assignment. Note: You must take all lab practical exams during the time/day it is given, there is no way to make-up a lab practical exam on days they are not given. Please note that lab quizzes are given at the beginning of the period and take about 15 minutes. If you are late, you will not be given extra time to complete your quiz. There are no make-up quizzes. If you miss a quiz, this will count as your dropped quiz. Participation and Professionalism in lab are critical to this course; therefore, these two parts of your grade will count for 10 points each. Attendance (which is part of participation) will be taken at the beginning of each lab period. Only 1 absence will be allowed for the semester, after which 5 points will be deducted for two absences and 10 points for three absences. Your 10 points for professionalism will remain intact as long as the rules and safety guidelines of the lab are followed. General Policy Academic dishonesty on exams or quizzes will result in a score of zero for that exam or quiz and could be cause for an instructor drop (See the campus policy regarding academic dishonesty). Do not give or accept help during an exam! Attendance: Orange Coast College policy on attendance states: "...a student who has been absent for 3 consecutive class meetings in a given section is to be dropped by the instructor. Excellent attendance and class participation will be taken into consideration during final grading. Students in need of accommodations in the college learning environment: Tutoring for many different programs including Microbiology and English Language are available in the Student Success Center (located near the Blue Clock Tower). Any student who feels he/she may need an accommodation based on the impact of a learning disability or other disability should contact the Disabled Students Center (DSC) to schedule an appointment.

3 Week Date General Microbiology Lecture Topics Assigned Chapters 1 T Feb 2 Introduction 1 R Feb 4 History & Nomenclature 1 2 T Feb 9 Chemistry 2 R Feb 11 Chemistry 2 Su Feb 14 ****Last Day to Withdraw to Avoid a W **** 3 T Feb 16 Chemistry 2 R Feb 18 Prokaryotes & Eukaryotes 4 4 T Feb 23 Prokaryotes & Eukaryotes 4 R Feb 25 Prokaryotes & Eukaryotes 4 5 T Mar 1 Prokaryotes & Eukaryotes 4 R Mar 3 Microbial Metabolism 5 6 T Mar 8 LECTURE EXAM 1 1, 2, 4 R Mar 10 Microbial Metabolism 5 7 T Mar 15 Microbial Growth 6 R Mar 17 Microbial Growth 6 8 T Mar 22 Control and Antimicrobial Drugs 7, 20 R Mar 24 Control and Antimicrobial Drugs 7, 20 T Mar 29 Spring Break, Campus Closed R Apr 31 Spring Break, Campus Closed 9 T Apr 5 Control and Antimicrobial Drugs 7, 20 R Apr 7 Microbial Genetics 8 10 T Apr 12 Microbial Genetics 8 R Apr 14 Microbial Genetics 8 11 T Apr 19 LECTURE EXAM 2 5, 6, 7, 20 R Apr 21 Fungi T Apr 26 Fungi 12 R Apr 28 Virology 13 Su May 1 ****Last Day to Drop**** 13 T May 3 Virology 13 R May 5 LECTURE EXAM 3 8, T May 10 Virology 13 R May 12 Microbial Pathogenesis T May 17 Innate Immunology 16 R May 19 Adaptive Immunology T May 24 Adaptive Immunology / Vaccines 17 R May 26 LECTURE EXAM 4 13, 15, 16, 17

4 WK Date Projected Lab Topics 1 M/T Feb 1/2 Orientation; Lab safety; Microscopy handout; 3-1 View slides of colored threads, blood smears, and the letter e Set up environmental plates (Incubate at 37 C) 2-1 W/R Feb 3/4 Wet mounts of cheek cells; Aseptic technique, Simple stain 1-3, 3-5 Check and discuss environmental plates (Observe Bacteria) 2-1; 2-2 *Continue growing environmental plates at room temp. 2 M/T Feb 8/9 Isolation of bacteria (Part I): Streak plate #1 1-4 Negative Stain; Gram stain 3-6; 3-7 W/R Feb 10/11 Check Streak Plate #1 and set up Streak Plate #2 1-4 Yeast and filamentous fungi: Tape mounts; Slide cultures (demo ) 12-1; 12-2 Check and discuss environmental plates (Observe Fungi) 2-1; M/T Feb 15/16 Holiday Washington s Birthday, No Lab W/R Feb 17/18 Quiz 1; Isolation of bacteria (Part II): Standard plate count 6-1 Endospore stain; Check Streak plate # Carbohydrate Catabolism/Fermentation; Respiration 4 M/T Feb 22/23 OF-glucose; PR-broth; Capsule stain; Acid-fast stain 5-2; 5-3; 3-9; 3-8 Readout for Standard plate count W/R Feb 24/25 IMViC (Indole/MRVP/Citrate) 5-20, 5-4, 5-8 Starch hydrolysis; ONPG 5-12; 5-18 Readouts for OF-glucose, PR-broth; Preparation of media (demo) M/T Feb 29/1 Quiz 2; Read results for IMViC, Starch hydrolysis, and ONPG W/R Mar 2/3 Lab Practical Exam #1 Protein Catabolism 6 M/T Mar 7/8 Decarboxylase (LDC, ODC); Phenylalanine; Urease; Casease 5-10; 5-11; 5-13; 5-14 TSI 5-21 (and 5-20) W/R Mar 9/10 Gelatinase; Motility Agar 5-15; 5-28 Readouts for Phenylalanine, Decarboxylase Urease, Casease, and TSI Respiration 7 M/T Mar 14/15 Anaerobic respiration: Anaerobic jar, Nitrate reduction 2-8, 5-7 Aerobic respiration: Catalase, Oxidase 5-5, 5-6 Readout for Decarboxylase, Gelatinase, Motility Agar W/R Mar 16/17 Set up Temperature exp.; ph demo; Osmotic pressure demo 2-9; 2-10; 2-11 Thioglycollate broth demo 2-7 Readout for the Anaerobic jar and Nitrate reduction *Remember antiseptics/disinfectants for next class Control of Microbial Growth 8 M/T Mar 21/22 Quiz 3; UV light; Disinfectants and antiseptics 2-13, 2-14 Antimicrobial susceptibility test (Kirby-Bauer method) 7-3 Readout for the Temperature experiment W/R Mar 23/24 View Protozoan slides 12-3 Read UV, Disinfectant/antiseptic, and Kirby-Bauer plates **Remember water samples for next class Lab Exercises

5 WK Date Projected Lab Topics M/T Mar 28/29 Spring Break, Campus Closed W/R Mar 30/31 Spring Break, Campus Closed Lab Exercises Microbes in Food/Water & Bacteria of the GI -Tract 9 M/T Apr 4/5 Set up Water Analysis: Membrane filter technique, LTB tubes 8-12; 8-13 Parasite video; View Protozoan slides (continued) 12-3 W/R Apr 6/7 Water Analysis (continued): BGLB tubes 8-13 Epidemiology experiment; Finish protozoan slides 7-6; 12-3 Readout for Membrane filters and LTB tubes Subculture Unknowns onto TSA Slants, Gram staining 10 M/T Apr 11/12 Quiz 4; Read results for Epidemiology and BGLB tubes 7-6; 8-13 Review W/R Apr 13 /14 Lab Practical Exam #2 Bacteria of the Skin, Respiratory Tract, and Mouth 11 M/T Apr 18/19 Nasal swab on MSA; Throat swab on blood agar 4-4; 5-25 Set up CAMP tests with Bacitracin test (A-disk), 5-26; 5-24 Optochin (O-disk); Bile Esculin 5-24; 4-3 Unknown project begins Streak onto NAP & MacConkey s agar 7-7, 4-5 W/R Apr 20/21 Nasal swab isolate: Coagulase test, Gram stain 5-27; 3-7 Throat swab isolate: Gram stain and identification handout Readout CAMP tests, Bile Esculin, View hemolysis patterns demo M/T Apr 25/26 Virus video; Set up Plaque Assay 6-5 Readout for coagulase, Work on unknown projects W/R Apr 27/28 Readout for Plaque Assay, Work on unknown projects Bacteria of the Urogenital Tract 13 M/T May 2/3 Urinalysis: Hurbi biplate 6-2 Genetics: Transformation 10-3 W/R May 4/5 Readouts for Hurbi biplate, Transformation View Helminth slides M/T May 9/10 Quiz 5; Blood smear differentials; Blood typing 11-1; 11-5 View Helminth slides (continued) 12-4 W/R May 11/12 Finish Helminth slides M/T May 16/17 Review; Finish/work on unknown projects W/R May 18/19 Final Lab Practical Exam (comprehensive) 16 M/T May 23/24 Clean up Lab W/R May 25/26 Finish all lecture material

6 SAFETY ISSUES IN THE BIOLOGY 210 MICROBIOLOGY LABORATORY 1. Do not come to lab late, new procedures (which often involve safety) are discussed at the beginning of lab. 2. Wear a lab coat at all times in the lab. Store your coat on the coat rack or in an assigned drawer. 3. Wear close-toed shoes at all times in the lab. Flip-flops and open-toed sandals are a hazard and are not permitted in the laboratory. 4. Be aware of Bunsen burner flames. Turn off burners when not in use. Secure long hair to prevent accidental exposure to open flames. Report burns or other injuries to your lab instructor immediately. 5. Use the eye wash station immediately, should any reagent accidentally get into your eyes. 6. Do not eat, drink, or store, food/drinks in the lab. 7. Place contaminated items in autoclavable containers for disposal. Do not mix glass with plastic items. 8. Use the biohazard bag to dispose of biological waste DO NOT use the trash basket to dispose of biological waste. 9. Remove old cultures from the refrigerator or incubator and place them in the biohazard bag for disposal. Remove labels from glassware when disposing. Do not remove cultures from the lab or place them in your lab drawer. 10. Report accidental spills of cultures to your instructor immediately. Do not attempt to pick up broken glass yourself. 11. Try to avoid bringing your hands to your mouth while in lab. 12. Wash your hands with soap and water before leaving lab. 13. Cover cuts on exposed skin with a sterile bandage. 14. Disinfect your desktop at the beginning and end of lab. 15. Inform your lab instructor if you are pregnant or if you are immunosuppressed as some lab exercises may pose unique problems in such cases. 16. Wear goggles or protective glasses when near ultraviolet lights. Use proper aseptic technique when making culture transfers.