PETAL. Training Courses. Promega Europe Training & Application Lab

Transcription

1 PETAL Training Courses 2016 CATALOG Promega Europe Training & Application Lab

2 ABOUT US Promega Europe Training & Application Lab (PETAL) offers training courses in molecular biology, cell biology, epigenetics, proteomics and genetic identity. Our training courses are designed to educate laboratory leaders, researchers, technicians and students about current techniques and provide the knowledge and skills required to implement these techniques in the laboratory. Our standard courses combine theoretical presentations with hands-on laboratory experience. PETAL instructors supplement presentations with case studies, exercises and discussions to encourage attendee participation. Training courses are also available to those who want a basic knowledge of various scientific topics. These courses are ideal for administrative and other staff members who want an overview of what takes place in the laboratory. The selection of training courses is intended to meet current research needs and is ever-evolving. Catalog training courses can be held at PETAL facilities or at your institution (assuming criteria such as access to the necessary laboratory space and equipment are met). PETAL is able to also offer customized training courses. An updated version of this catalog is available at:

3 TABLE OF CONTENTS Training Courses: 2016 Training Agenda 4 Molecular Biology Introduction to Molecular Biology 6 Molecular Biology Fundamentals 7 PCR and Reverse Transcription PCR Technologies 8 Real-Time PCR Technologies 9 Cell Biology Cell Line Identification 11 Cell Culture: Theory and Practice 12 Cell Culture to Cell Analysis 13 Epigenetics Epigenetics: Techniques and Applications 15 Proteomics Introduction to Proteomics 17 HaloTag Technology 18 Genetic Identity Introduction to Genetic Identity 20 From DNA to Genetic Profile Analysis 21 Terms and Conditions: Training Course Information Registration, Cancellation and Payment 23 Registration Form 25 Map and Directions 26 22

4 2016 TRAINING CALENDAR Topic Length Dates Introduction to Molecular Biology Molecular Biology Fundamentals PCR and Reverse Transcription PCR Technologies Real-Time PCR Technologies Cell Line Identification Cell Culture: Theory and Practice Cell Culture to Cell Analysis 1 Day 3 Days 1 Day 1 Day 1 Day 3 Days 5 Days In English: Jan 19, July 29 In French: June 15, Nov 9 In English: Jan 13-15, July In French: June 15-17, Dec In English: Jan 21, July 26 In French: June 14, Nov 10 In English: Jan 20-22, Sept In French: July 18-20, Nov 7-9 In English: Jan 20, Sept 15 In French: July 25, Nov 15 In English: Jan 25-27, Sept In French: June 8-10, Nov In English: Feb 1-5, July 4-8 In French: May 23-27, Dec Epigenetics: Techniques and Applications 3 Days In English: July Introduction to Proteomics HaloTag Technology Introduction to Genetic Identity From DNA to Genetic Profile Analysis Customized Training Courses 1 Day 2 Days 1 Day 2 Days TBD In English: Jan 29, Sept 9 In French: July 12, Nov 14 In English: Mar 28-29, Sept 8-9 In French: June 6-7 In English: Jan 18, Dec 15 In French: June 13, Nov 21 In English: Apr 7-8, Sept In French: July 11-12, Nov To Be Determined (TBD) 4

5 Molecular Biology

6 Introduction to Molecular Biology Audience: 1 Day (7 hours) Anyone who wants to learn or review basic molecular biology principles and associated techniques. Goals: Jan 19, July 29 English June 15, Nov 9 French 1. Acquire or review basic knowledge of molecular biology. 2. Learn molecular biology techniques. 3. Perform basic experiments in molecular biology. 250 /students 350 /others Lunch and teaching materials included Charbonnières les bains, France Program: Class Discussion Molecular Biology Principles Structure and function of nucleic acids (DNA and RNA) Molecular Biology Techniques Nucleic acid extraction and analysis methods Amplification (PCR and real-time PCR) Molecular cloning, sequencing and protein expression Lab Activity Extraction of nucleic acids, electrophoresis and analysis Case study: molecular cloning 6

7 Molecular Biology Fundamentals Audience: 3 Days (21 hours) Anyone including technicians, research assistants and researchers, who wants to learn about molecular biology and associated techniques. Goals: Jan July English June Dec French 1. Acquire or review basic knowledge of molecular biology. 2. Learn and perform different molecular biology techniques. 3. Solve the most common experimental problems. 4. Learn how to optimize protocols. Program: 1200 /students 1400 /others Lunch and teaching materials included Class Discussion Molecular Biology Principles Structure and function of nucleic acids (DNA and RNA) DNA/RNA modification enzymes Protein Synthesis DNA replication, transcription and translation Charbonnières les bains, France Molecular Biology Techniques Nucleic acid extraction and analysis methods Amplification (PCR, RT-PCR, real-time PCR) Molecular cloning, sequencing and protein expression Lab Activity Molecular cloning, PCR and protein expression Manual and automated extraction of nucleic acids, electrophoresis and analysis. 7

8 PCR & Reverse Transcription PCR Technologies Audience: 1 Day (7 hours) Anyone including technicians, research assistants and researchers, who wants to learn about PCR and RT-PCR. Jan 21, July 26 English June 14, Nov 10 French Goals: 1. Acquire or review basic knowledge of PCR and Reverse Transcription PCR (RT-PCR) techniques. 2. Identify key points for optimization. 3.Practice primer design. 300 /students 400 /others Lunch and teaching materials included 4. Perform RT-PCR. Program: Class Discussion PCR Different PCR methods and associated polymerases RT-PCR RT-PCR characteristics, principle and methods PCR Optimization and Primer Design Key points Charbonnières les bains, France Lab Activity RT-PCR and analysis of results 8

9 Real-Time PCR Technologies Audience: 3 Days (21 hours) Anyone including technicians, research assistants and researchers who wants to learn or deepen their knowledge of real-time PCR. Goals: Jan Sept English July Nov 7-9 French 1200 /students 1400 /others Lunch and teaching materials included Charbonnières les bains, France 1. Refresh basic knowledge of molecular biology. Learn basics of real-time PCR. 2.Perform real-time PCR and analyze results. 3. Learn key points of qpcr and RT-qPCR optimization. 4. Be able to solve common qpcr problems. Program: Class Discussion Nucleic acids: definition, structure and extraction methods PCR and qpcr: description and comparison qpcr techniques: description and comparison Data analysis Multiplex qpcr Lab Activity Nucleic acid extraction Two different qpcr techniques Analysis and discussion of results 9

10 Cell Biology

11 Cell Line Identification Audience: 1 Day (7 hours) Anyone including technicians, research assistants and researchers, who wants to learn about cell line identification. Goals: Jan 20, Sept 15 English July 25, Nov 15 French 1. Understand the importance and challenges of cell line identification. 2. Learn the basics of STR analysis. 3. Perform cell line identification. 4. Learn how to analyze results. 400 /students 500 /others Lunch and teaching materials included Program: Class Discussion Importance of cell line identification Method principles, including STR technology Lab Activity Perform cell line identification: from cell to STR profile Charbonnières les bains, France 11

12 Cell Culture: Theory and Practice Audience: 3 Days (21 hours) Anyone who wants to learn about cell culture and other common cell biology techniques. Note: The number of attendees is limited to 8 per class. Jan Sept English June 8-10 Nov French Goals: 1. Acquire or review basic knowledge of cell biology and cell culture. 2. Learn about cell biology techniques. 3. Practice basic techniques. 4. Learn how to optimize cell culture protocols /students 1400 /others Lunch and teaching materials included Program: Class Discussion The cell: structure and cell cycle Transfection methods and optimization Cell viability: definition, benefits and optimization The basics of cell culture and the importance of a sterile environment Lab Activity Cell culture: maintaining and passaging a cell line Transfection and analysis of results Charbonnières les bains, France Multiplex viability and cytotoxicity assays and analysis of results 12

13 Cell Culture to Cell Analysis Audience: 5 Days (35 hours) Feb 1-5 July 4-8 English May Dec French Anyone who needs a basic knowledge of cell culture and other common cell biology techniques. Note: The number of attendees is limited to 8 per class. Goals: 1. Acquire or review basic knowledge of cell biology and cell culture. 2. Practice common and advanced cell biology techniques. 3.Analyze results and optimize cell culture protocols /students 2000 /others Lunch and teaching materials included Charbonnières les bains, France Program: Class Discussion Basics of cell culture and the importance of a sterile environment Transfection methods and optimization Cell viability: definition, benefits and optimization Cell lines: creation, freezing and storage Signal transduction Use of reporter genes in cell culture Cell line identification Cell imaging Lab Activity Cell culture: importance of a sterile environment and Good Laboratory Practice (GLP) Transfection and analysis of results Multiplex viability and cytotoxicity assays and analysis of results Use of reporter genes Cell imaging 13

14 Epigenetics

15 Epigenetics: Techniques and Applications Audience: 3 Days (21 hours) Anyone including research assistants and researchers, who wants to learn about epigenetics and associated techniques. Goals: 1.Review the epigenetics field. 2. Describe the different techniques available. July English 3. Acquire practical knowledge in some of the epigenetics techniques. 4. Provide research highlights. Program: 1500 /students 1800 /others Lunch and teaching materials included Class Discussion Introduction to Epigenetics Epigenetics field, epigenome & chromatin structure Factors influencing epigenetics: inheritance, environment Technologies DNA modifications, histone modifications, and RNA analysis methods Set up epigenetics experiments (e.g. key points and optimization) Research Most impactful epigenetic research stories from 2013/2014 Charbonnières les bains, France Lab Activity BiSulfite conversion of DNA Histone deacetylation assay BRET protein:protein interaction assay HaloCHIP TM System 15

16 Proteomics

17 Introduction to Proteomics Audience: 1 Day (7 hours) Anyone who wants to learn about proteomics. Goals: 1. Acquire or review basic knowledge of proteomics. Jan 29, Sept 9 English July 12, Nov 14 French 2. Learn proteomics techniques. 3. Perform basic techniques. 4. Learn how to optimize protocols. 400 /students 500 /others Lunch and teaching materials included Program: Class Discussion Common Challenges in Protein Analysis Overview of Proteomics Techniques Protein purification Mass spectrometry Protein analysis and imaging Lab Activity Cell-free protein expression Charbonnières les bains, France 17

18 HaloTag Technology Audience: 2 Days (14 hours) Anyone including technicians, research assistants and researchers, who wants to learn about the use of HaloTag technology. Jan Sept 8-9 English June 6-7 French Goals: 1. Understand HaloTag technology and its possibilities. 2.Practice with HaloTag technology. 3. Optimize protocols. 800 /students 1000 /others Lunch and teaching materials included Program: Class Discussion HaloTag Technology Principles Applications Protein purification, protein interaction discovery, protein arrays, cell-based assays for protein translocation, receptor internalization, protein stability... Lab Activity Protein purification Cell imaging Charbonnières les bains, France 18

19 Genetic Identity

20 Introduction to Genetic Identity Audience: 1 Day (7 hours) Anyone who wants to learn about genetic identity. Goals: Jan 18, Dec 15 English June 13, Nov 21 French 1. Understand the challenges of genetic identity. 2. Learn the basics of STR analysis. 3. Perform a genetic profile. 4. Learn to analyze STR results. 400 /students 500 /others Lunch and teaching materials included Program: Class Discussion From DNA to genetic profile: forensic lab challenges and profiling workflow STR technology introduction Lab Activity Generate an STR profile; from PCR to data analysis. Charbonnières les bains, France 20

21 From DNA to Genetic Profile Analysis Audience: 2 Days (14 hours) Anyone including researchers, technicians and students, who wants to learn about the use of STRs for genetic identification. April 7-8 Sept English July Nov French Goals: 1. Understand the challenges of genetic identification. 2. Learn the basics of STR analysis. 3. Generate a DNA profile. 800 /students 1000 /others Lunch and teaching materials included 4. Learn to analyze results. Program: Class Discussion From DNA to genetic profile: forensic lab challenges and profiling workflow STR Technology Optimization and troubleshooting Validation of STR systems Lab Activity DNA purification Charbonnières les bains, France Generate a DNA profile; from PCR to data analysis 21

22 TRAINING COURSE INFORMATION Training Courses All courses take place at PETAL unless otherwise arranged by special request. Students are welcome at PETAL as early as 8:30am. The first day of training will begin at 9:30am. Training courses will end at 5:00pm except for one-day courses which end at 5:30pm. During the year, additional dates may be offered if the number of participants justifies it. Please contact us or visit our website at: Training courses are conducted in French or English as indicated in the catalog. Places are limited and reserved upon payment only. Customized Training Courses Customized training courses are defined and planned as requested. Training courses can take place at PETAL or at a site chosen by the customer. PETAL staff will collect information about training needs, then design a program to meet the expectations of attendees. Training courses can be conducted in either French or English.

23 REGISTRATION You can register by mail, , fax or internet. Complete the registration form at the end of this brochure or visit our website at: Once your registration is received, you will get a confirmation and a quote. Registration is confirmed upon receipt of your payment. Please note that a course may be canceled if the number of participants is insufficient. TRAINING COURSE AGREEMENT A training course agreement is established between your institution and PETAL, in accordance with current laws. A certificate of attendance is awarded at the end of the course. PETAL is registered under the number CANCELLATION Cancellation requests must be sent by mail, or fax. If the cancellation notice is received more than 30 days before the training course, fees will be fully refunded. If the cancellation notice is received days before the training, a cancellation fee of 10% will be withheld. If the cancellation notice is received 7 14 days before the training, a cancellation fee of 50% will be withheld. If the cancellation notice is received less than 7 days before the training, no refund will be given. In lieu of cancellation, you may transfer your registration to a colleague. Training courses may be postponed at no charge. 23

24 PAYMENT Prices exclude VAT. They include attendance at the training course, teaching materials, coffee breaks and lunch. Prices do not include travel costs and accommodation. Please address all documents related to billing and payment, including purchase orders and agreements to the address below. Purchase orders may be sent by fax or . Payment must be made by a bank transfer through the Promega France account: Bank : BNP PARIBAS Account Number: IBAN FR BIC: BNPAFRPPLPD SIRET N : APE: 4675Z VAT Code: FR CONTACT US Promega France PETAL 24 Chemin des Verrières F Charbonnières les Bains phone: +33 (0) fax: +33 (0) petal.training@promega.com STAY IN TOUCH Facebook: promegapetal 24

25 REGISTRATION FORM Training Course Name Training Course Date Price + VAT (20% as of 01/01/2014) Registration is confirmed upon receipt of payment. Payment must be made by bank transfer to Promega France account. IBAN: FR ; BIC: BNPAFRPPLPD. Bank: BNP PARIBAS One day of training course is equivalent to 7 hours. To view our cancellation policy, see page 23. Full Name Phone Institution Address of Institution Position Level of Scientific Experience (Theoretical) Level of Scientific Experience (Practical) Signature/Stamp Date

26 86 Arriving from Lyon 86 Going to Lyon

27 DIRECTIONS Public transportation From Part-Dieu station, take the Metro Line B toward Stade de Gerland. Stop at Saxe Gambetta. Take Line D toward Gare de Vaise. Stop at Gorge de Loup. Take bus #86 toward La Tour de Salvagny. Stop at Charbonnières les Verrières. By car From Lyon or A7: Join the Autoroute du Soleil A6/E15. Take exit 36, and continue on the ring road toward Tassin la Demi Lune/ Charbonnières. Take Route de Paris/DS307. At the roundabout, continue straight on Route de Paris for 2.5km. Turn left on chemin des Verrières, then turn at the first right. PETAL is after the roundabout. From A6: Take exit 33 (Limonest/Dardilly-Porte de Lyon), and continue on road D306. At the roundabout, take road RN6 for 1.3km. Turn right on road N489 toward La Tour de Salvagny, then turn left on RN7 toward Charbonnières les Bains. When entering Charbonnières, turn right on chemin des Verrières, then turn at the first right. PETAL is after the roundabout. 27

28 PETAL Training Courses 2016 CATALOG Promega Europe Training & Application Lab Tel: + 33 (0) Fax: + 33 (0) petal.training@promega.com PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711, USA PROMEGA CORPORATION ALL RIGHTS RESERVED PRICES & SPECIFICATIONS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE PROJECT # PART #BR267