PRODUCT CATALOGUE BIOCHEK ANIMAL HEALTH MONITORING SYSTEM

Transcription

1 PRODUCT CATALOGUE BIOCHEK ANIMAL HEALTH MONITORING SYSTEM 1 1

2 INTRODUCTION BIOCHEK Monitoring the health status of animals is essential in order to improve the health, productivity of livestock. BioChek is a veterinary diagnostic company that provides its customers with an accurate, reliable and easy-to-use animal health monitoring system for the Poultry and Swine industries. This system consists of a broad range of ELISA and PCR Test Kits, the BioChek ELISA Assay Robot (BEAR), and the BioChek Monitoring Software. The ELISA and PCR Test Kits provide information about the health status of swine herds and poultry flocks, which allows producers to take corrective action when the assay(s) indicate(s) a health problem. The BEAR generates highly reproducible results in a very cost effective way. When samples have been processed by the BEAR the test results will be transferred to the BioChek Cloud Software package where all data is accessible anytime anywhere 24/7. The software package can generate standardized individual reports and create herd or flock trend reports comparing the latest test results with previous test results. All of this can be done either from behind your desktop or on your mobile phone. BioChek was founded in 1997 and operates in most countries with a manufacturing facility in the UK, a USDA licensed facility in the US and the commercial head office in the Netherlands. BioChek has a strong reputation in service. Our technical support ensures ease of use for veterinary laboratories and accurate test results for end users. This catalogue offers a complete overview of BioChek products and services. For questions concerning these products or requests for more detailed product information such as detail aids, interpretation guides, or marketing materials, please contact your BioChek representative or BioChek headquarters

3 TABLE OF CONTENTS Introduction BioChek Quick Product Reference ELISA Test Kits Swine ELISA reference controls & accessories Swine PCR Test Kits Swine PCR standards & accessories Swine ELISA Test Kits Poultry ELISA reference controls & accessories Poultry PCR Test Kits Poultry PCR standards & accessories Poultry Equipment Software Labtraining

4 QUICK PRODUCT REFERENCE ELISA TEST KITS SWINE Catalogue number Page number CSF ELISA SK106 9 Hps ELISA SK104 9 Mhyo ELISA SK108 9 PCV2 ELISA SK PRRS ELISA SK PRVg1 ELISA SK PRVgB ELISA SK Salm Gp B/C1/D ELISA SK InfA Multi ELISA CK ELISA REFERENCE CONTROLS & ACCESSORIES SWINE Catalogue number Page number Swine Pre-diluted Reference Control SR BioChek Partial Plate kit set BC PP 12 PCR TEST KITS SWINE Catalogue number Page number App PCR SP Hps PCR SP Myco PCR SP PCV2 PCR SP PRRS PCR SP Campy PCR MP InfA PCR MP Salmonella PCR MP Spp-Se-St PCR MP

5 PCR STANDARDS & ACCESSORIES SWINE Catalogue number Page number PCR standards App SP PCR standards Hps SP PCR standards Myco SP PCR standards PCV2 SP PCR standards PRRS SP PCR standards Campy MP PCR standards InfA MP PCR standards Salmonella MP PCR standards Spp-Se-St MP Nucleic Acid Extraction Kit BP Lysis Buffer A (100 reactions) BP

6 QUICK PRODUCT REFERENCE ELISA TEST KITS POULTRY Catalogue number Page number AE ELISA CK AI ELISA CK InfA Multi ELISA CK AI (ag) ELISA CK ALV (ag) ELISA CK422B 22 ART ELISA CK BLS ELISA CK CAstV ELISA CK CAV ELISA CK EDS ELISA CK FAdV ELISA CK IBD ELISA CK IBV ELISA CK ILT ELISA CK Mg ELISA CK Ms ELISA CK Mg/Ms ELISA CK Mm ELISA CK NDV ELISA CK NDV-F ELISA CK ORT ELISA CK REO ELISA CK REV ELISA CK Salm Gp D ELISA CK Salm Gp B ELISA CK Salm Gp B/D ELISA CK ELISA REFERENCE CONTROLS & ACCESSORIES POULTRY Catalogue number Page number Pre-diluted Reference Control CR Freeze Dried Reference Serum CR Freeze Dried Negative Reference Serum CR ALV Pre-diluted Reference Control CR BioChek Partial Plate kit set BC PP

7 PCR TEST KITS POULTRY Catalogue number Page number ILT PCR CP Mg-Ms PCR CP NDV PCR CP ORT PCR CP Salmonella PCR MP Spp-Se-St PCR MP Se-St-Sh PCR CP Campy PCR MP InfA PCR MP PCR STANDARDS & ACCESSORIES POULTRY Catalogue number Page number PCR standards ILT CP PCR standards Mg-Ms CP PCR standards NDV CP PCR standards ORT CP PCR standards Salmonella MP PCR standards Spp-Se-St MP PCR standards Se-St-Sh CP PCR standards Campy MP PCR standards InfA MP Nucleic Acid Extraction Kit BP Lysis Buffer A (100 reactions) BP

8 PRODUCT CATALOGUE SWINE 8 8

9 ELISA TEST KITS - SWINE The BioChek ELISA Test Kits are specific for detecting antibodies or antigens against various diseases common in pigs. Each kit comes with 5 test plates, conjugate, diluent, substrate, wash buffer and positive and negative controls. The results are given in titers allowing a quantitative interpretation of results. CSF ELISA Catalogue number: SK106 Tests/kit: 480 tests (5 strip plates) Classical Swine Fever Antibody test kit, strip plate format The Classical Swine Fever Antibody test kit will measure the number of antibodies against protein E2 of the Classical Swine Fever Virus (Pesti Virus) in the serum of pigs. Serum is diluted at 1:30. Short test incubation is minutes. A longer overnight incubation of diluted samples may be used for increased sensitivity. Test reads at 405 nm wavelength. Hps ELISA Catalogue number: SK104 Tests/kit: 192 tests (2 plates) Haemophilus parasuis Antibody test kit, strip plate format The Haemophilus parasuis Antibody test kit will measure the amount of antibodies to OppA in the serum of pigs. OppA antibodies are present in herds clinically infected with Haemophilus parasuis. Serum is diluted at 1:200. Test incubation is minutes. Test reads at 405 nm wavelength. Mhyo ELISA Catalogue number: SK108 Tests/kit: 480 tests (5 plates) Mycoplasma hyopneumoniae Antibody test kit, strip plate format The Mycoplasma hyopneumoniae Antibody test kit will measure the amount of antibodies to Mycoplasma hyopneumoniae present in the serum of pigs. Serum is diluted at 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. 9

10 ELISA TEST KITS - SWINE PCV2 ELISA Catalogue number: SK105 Tests/kit: 480 tests (5 plates) Porcine Circovirus type 2 Antibody test kit, strip plate format The Porcine Circovirus type 2 Antibody test kit will measure the amount of antibodies to all types of PCV2 present in the serum of pigs. Serum is diluted at 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. PRRS ELISA Catalogue number: SK103 Tests/kit: 480 tests (5 plates) Porcine Respiratory Reproductive Syndrome Virus Antibody test kit, strip plate format The Porcine Respiratory Reproductive Syndrome Virus Antibody test kit detects antibodies to US, high pathogenic Asian and European PRRS Virus strains. Serum samples are diluted at 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. PRVg1 ELISA Catalogue number: SK102 Tests/kit: 480 tests (5 plates) Aujeszky g1 Antibody test kit, strip plate format The Aujeszky g1 Antibody test kit is a qualitative blocking ELISA for the detection of antibodies to glycoprotein 1 (g1) of Aujeszky s disease Virus (Pseudorabies Virus (PRV)) in the serum of pigs. The Aujeszky g1 ELISA makes it possible to differentiate between pigs with field infection and pigs vaccinated with a ge deleted vaccine (DIVA). Serum samples are diluted at 1:2. Test incubation is minutes in a humid environment. Test reads at 405 nm wavelength

11 ELISA TEST KITS - SWINE PRVgB ELISA Catalogue number: SK109 Tests/kit: 480 tests (5 plates) Aujeszky gb Antibody test kit, strip plate format The Aujeszky gb (Pseudorabies Virus) Antibody test kit will measure the amount of antibodies to Pseudorabies Virus gb in the serum of pigs. The Aujeszky gb test kit detects antibodies in both infected and vaccinated pigs. The test is generally used to confirm the negative PRV status of a herd (to confirm freedom from disease). Serum is diluted at 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. Salm Gp B/C1/D ELISA Catalogue number: SK101 Tests/kit: 480 tests (5 plates) Salmonella Group B, C1 and D Antibody test kit, strip plate format Salmonella Group B,C1 and D Antibody test kit will measure the amount of antibodies to invasive group B, C1 and D Salmonella species in the serum or meat juice of pigs. The test has been optimised for use in Salmonella monitoring programmes. Serum is diluted at 1:20, while meat juice is diluted at 1:2. Test incubation is minutes. Test reads at 405 nm wavelength. InfA Multi ELISA Catalogue number: CK401 Tests/kit: 480 tests (5 plates) Multispecies Influenza A Antibody test kit, solid plate format The Multispecies Influenza A Antibody test kit will determine the presence of antibodies to Influenza Type A in the serum of any animal, including pigs. Serum dilution is 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. 11

12 ELISA REFERENCE CONTROLS & ACCESSORIES - SWINE Swine Pre-diluted Reference Control Catalogue number: SR700 Tests/kit: 5 ml / vial The Swine Pre-diluted Reference Control SR700 is a positive control with known titers for PRRS, Hps, PCV2, Mhyo, InfA Multi, CSF, PRVgB, PRVg1 and Salmonella. By using this reference control, all steps of the analysis are checked, except for the dilution step. BioChek Partial Plate kit set Catalogue number: BC PP The Partial Plate kit set contains all the necessary instructions and materials (plate sealers, sealable storage pouches, desiccant pouches) for running and storing partial test plates. The correct use of the BC PP kit set prevents the decay of coated antigen plates, extending the storage life of partially used test plates to 2-6 weeks after first use

13 PCR TEST KITS - SWINE The BioChek quantitative Polymerase Chain Reaction (PCR) Test Kits will detect the presence of target specific DNA/RNA. Every PCR test kit uses the same thermo/cycler protocol, thus enabling the technician to perform PCR reactions for different targets at the same time on one thermocycler. All BioChek PCR kits are validated for use with a variety of thermocyclers (For a complete list of compatible PCR thermocyclers please contact BioChek) and a total NA extraction kit, sold separately. Results are given in Cq values, allowing differentiation between low, medium and high loads of antigen. All necessary controls are included (Negative Control, Positive Control and Internal Control). App PCR Catalogue number: SP104 Tests/kit: 100 tests Actinobacillus Pleuropneumoniae DNA Test Kit The Actinobacillus Pleuropneumoniae DNA Test Kit will detect the presence of Actinobacillus pleuropneumoniae via genes that code for the toxins App can produce: Apx IV and/or Apx I and/or Apx III in extracts from oral fluids, nasal swabs or tissue. Primers and probes are specific for the genes coding for toxins Apx IV, ApxI and Apx III. Test duration after DNA extraction is approximately 90 minutes. Test reads at Fam (Apx IV), CY5 (Apx I), Texas Red (Apx III) and HEX (IC) channels. For quantification of results, PCR standards App are available. Hps PCR Catalogue number: SP103 Tests/kit: 100 tests Haemophilus Parasuis DNA Test Kit The Haemophilus Parasuis DNA Test Kit will detect simultaneously the presence of DNA from Haemophilus parasuis and/or virulence factor vtaa10 in extracts from oral fluids, nasal swabs or tissue. Test duration after DNA extraction is approximately 90 minutes. Test reads at Fam (Hps), Texas Red (vtaa10) and CY-5 (IC) channels. For quantification of results, PCR standards Hps are available. Myco PCR Catalogue number: SP102 Tests/kit: 100 tests Mycoplasma Hyopneumoniae-Hyorhinis-Hyosynoviae DNA Test Kit The Mycoplasma Hyopneumoniae-Hyorhinis-Hyosynoviae DNA Test Kit allows for the simultaneous detection of Mycoplasma hyopneumoniae and/or Mycoplasma hyorhinis and/or Mycoplasma hyosynoviae. The test will determine the presence and load of antigen in a variety of samples of pigs. Test duration after DNA extraction is approximately 90 minutes. Test reads at FAM, HEX (VIC), Texas Red and CY5 (Quasar 670) channels. For quantification of results, Myco PCR standards are available. 13

14 PCR TEST KITS - SWINE PCV2 PCR Catalogue number: SP101 Tests/kit: 100 tests Porcine Circovirus Type 2 DNA Test Kit The Porcine Circovirus Type 2 DNA Test Kit allows for the detection of Porcine Circovirus type 2. The test will determine the presence and load of antigen in the serum or oral fluid of pigs. Test duration after DNA extraction is approximately 90 minutes. Test reads at FAM and CY5 (Quasar 670) channels. For quantification of results, PCV2 PCR standards are available. PRRS PCR Catalogue number: SP105 Tests/kit: 100 tests Porcine Respiratory Reproductive Syndrome RNA Test Kit The Porcine Respiratory Reproductive Syndrome RNA Test Kit will detect the presence and load of antigen for the Porcine Respiratory Reproductive Syndrome in serum, oral fluids and tissues. Test duration after DNA extraction is approximately 100 minutes. Test reads at Fam (type2), CY5 (IC) and HEX (type 1) channels. For quantification of results, PCR standards PRRS are available. Campy PCR Catalogue number: MP103 Tests/kit: 100 tests Campylobacter Coli-Jejuni-Lari DNA Test Kit The Campylobacter Coli-Jejuni-Lari DNA Test Kit allows for the simultaneous detection of Campylobacter coli, Campylobacter jejuni and Campylobacter lari. Test duration after DNA extraction is approximately 90 minutes. Test reads at Fam, CY5 (Quasar 670), HEX and Texas Red channels. For quantification of results, PCR standards Campy are available. InfA PCR Catalogue number: MP101 Tests/kit: 100 tests Influenza A RNA Test Kit The Influenza A RNA Test Kit allows for the detection of Influenza A. The test will determine the presence and load of antigen in tissues, swabs or (oral) fluids of any type of animal. Test duration after RNA extraction is approximately 100 minutes. Test reads at Fam and CY5 (Quasar 670) channels. For quantification of results, PCR standards InfA are available

15 PCR TEST KITS - SWINE Salmonella PCR Catalogue number: MP104 Tests/kit: 100 tests Salmonella Species DNA Test Kit The Salmonella Species DNA Test Kit allows for the detection of all Salmonella species. The test will determine the presence and load of antigen in faecal samples, meat samples or dust samples after pre-enrichment in suitable media. Test duration after DNA extraction and enrichment is approximately 90 minutes. Test reads at Fam and HEX channels. For quantification of results, PCR standards Salmonella are available. Spp-Se-St PCR Catalogue number: MP102 Tests/kit: 100 tests Salmonella Species-Enteritidis-Typhimurium DNA Test Kit The Salmonella Species-Enteritidis-Typhimurium DNA Test Kit allows for the simultaneous detection of Salmonella spp, Salmonella enteritidis and Salmonella typhimurium. The test will determine the presence and load of antigen in faecal samples, meat samples or dust samples after pre-enrichment in suitable media. Test duration after DNA extraction and enrichment is approximately 90 minutes. Test reads at Fam, CY5 (Quasar 670), HEX and Texas Red channels. For quantification of results, PCR standards Spp-Se-St are available. 15

16 PCR STANDARDS & ACCESSORIES - SWINE PCR standards App Catalogue number: SP vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Actinobacillus pleuropneumoniae DNA test kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Hps Catalogue number: SP203 8 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Haemophilus parasuis DNA test kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results

17 PCR STANDARDS & ACCESSORIES - SWINE PCR standards Myco Catalogue number: SP vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Mycoplasma Hyopneumoniae-Hyorhinis-Hyosynoviae DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check performance of the PCR and to quantify results. PCR standards PCV2 Catalogue number: SP201 4 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Porcine Circovirus type 2 DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards PRRS Catalogue number: SP205 8 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Porcine Respiratory Reproductive Syndrome RNA test kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Campy Catalogue number: MP vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Campylobacter Coli-Jejuni-Lari DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards InfA Catalogue number: MP201 4 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Influenza A RNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. 17

18 PCR STANDARDS & ACCESSORIES - SWINE PCR standards Salmonella Catalogue number: MP204 4 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Salmonella Species DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Spp-Se-St Catalogue number: MP vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Salmonella Species-Enteritidis-Typhimurium DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. Nucleic Acid Extraction Kit Catalogue number: BP801 Tests/kit: 100 extractions The Nucleic Acid Extraction Kit (spin columns) allows for the extraction and purification of Nucleic Acid from a variety of samples (serum, swabs, oral fluids, tissue etc.) and can be used with all BioChek PCR kits and can be stored at room temperature. Not provided: 96% ethanol. Lysis Buffer A Catalogue number: BP900 2 reagents (100 reactions) Lysis Buffer A is a complete set of reagents to perform DNA extraction (not suitable for RNA extraction). The only equipment that is needed is a heating block. The buffer is validated for Mg-Ms and Salmonella extraction. Check with the technical staff from BioChek for which other targets and matrices it can be used and for further specific details

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20 PRODUCT CATALOGUE POULTRY 20 20

21 ELISA TEST KITS - POULTRY The BioChek ELISA tests are specific for detecting antibodies or antigens to various diseases common in chickens and turkeys. Each kit comes with 5 test plates, conjugate, diluent, substrate, wash buffer and positive and negative controls. The results are given in titers allowing quantitative interpretation of results. AE ELISA USDA LICENSED Catalogue number: CK123 Tests/kit: 480 tests (5 plates) Avian Encephalomyelitis Virus Antibody test kit, solid plate format The Avian Encephalomyelitis Antibody test kit will measure the amount of antibodies to the Avian Encephalomyelitis Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. AI ELISA USDA LICENSED Catalogue number: CK121 Tests/kit: 480 tests (5 plates) Avian Influenza Antibody test kit, solid plate format The Avian Influenza Antibody test kit will measure the amount of of antibodies to Avian Influenza Virus type A. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. InfA Multi ELISA Catalogue number: CK401 Tests/kit: 480 tests (5 plates) Multispecies Influenza A Antibody test kit, solid plate format The Multispecies Influenza A Antibody test kit will determine the presence of antibodies in the serum of any animal to Influenza Virus Type A. Serum dilution is 1:50. Test incubation is minutes. Test reads at 405 nm wavelength. AI (ag) ELISA Catalogue number: CK301 Tests/kit: 192 tests (2 plates) Avian Influenza Antigen test kit, solid plate format The Avian Influenza Antigen detection test kit will determine the presence of nucleoprotein from Type A Avian Influenza via tracheal or cloacal swab samples. Swabs are diluted in 1 ml PBS. Test incubation is minutes. Test reads at 405 nm wavelength. 21

22 ELISA TEST KITS - POULTRY ALV (ag) ELISA Catalogue number: CK422B Tests/kit: 480 tests (5 plates) Avian Leukosis Virus Antigen test kit, solid plate format The Avian Leukosis Virus Antigen test kit will determine the presence of p27 antigen of the Avian Leukosis Virus in chickens. The test will determine the presence of ALV p27 antigen. Meconium or cloacal swab samples are diluted in 1 ml PBS. Albumen samples are added undiluted to the micro titer wells. Test incubation is minutes. Test reads at 405 nm wavelength. ART ELISA Catalogue number: CK120 Tests/kit: 480 tests (5 plates) Avian Rhinotracheitis Antibody test kit, solid plate format The Avian Rhinotracheitis Antibody test kit will measure the amount of antibodies to the Avian Pneumovirus. The test will determine the presence of antibodies in the serum of chickens and turkeys. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. BLS ELISA Catalogue number: CK131 Tests/kit: 480 tests (5 plates) Big Liver and Spleen Disease Antibody test kit, solid plate format The Big Liver and Spleen Disease Antibody test kit will measure the amount of antibodies to the Avian Hepatitis E Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. CAstV ELISA Catalogue number: CK133 Tests/kit: 480 tests (5 plates) Chicken Astrovirus Group B Antibody test kit, solid plate format The Chicken Astrovirus Antibody test kit will measure the amount of antibodies to the Chicken Astrovirus Group B. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. CAV ELISA USDA LICENSED Catalogue number: CK126 Tests/kit: 480 tests (5 plates) Chicken Anemia Virus Antibody test kit, solid plate format The Chicken Anemia Virus Antibody test kit will measure the amount of antibodies to the Chicken Anemia Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength

23 ELISA TEST KITS - POULTRY EDS ELISA Catalogue number: CK112 Tests/kit: 480 tests (5 plates) Egg Drop Syndrome Virus Antibody test kit, solid plate format The Egg Drop Syndrome Virus Antibody test kit will measure the amount of EDS antibodies to the Egg Drop Syndrome Virus (Adenovirus group III). The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. FAdV ELISA Catalogue number: CK132 Tests/kit: 480 tests (5 plates) Fowl Adenovirus Group 1 Antibody test kit, solid plate format The Fowl Adenovirus Group 1 Antibody test kit will measure the amount of antibodies to group 1 of the Fowl Adenovirus. The test will determine the presence of FAdV Group 1 antibodies in the serum of chickens. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. IBD ELISA USDA LICENSED Catalogue number: CK113 Tests/kit: 480 tests (5 plates) Infectious Bursal Disease Virus Antibody test kit, solid plate format The Infectious Bursal Disease Virus Antibody test kit will measure the amount of antibodies to the Infectious Bursal Disease Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. IBV ELISA USDA LICENSED Catalogue number: CK119 Tests/kit: 480 tests (5 plates) Infectious Bronchitis Virus Antibody test kit, solid plate format The Infectious Bronchitis Virus Antibody test kit will measure the amount of antibodies to the Infectious Bronchitis Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. 23

24 ELISA TEST KITS - POULTRY ILT ELISA USDA LICENSED Catalogue number: CK124 Tests/kit: 480 tests (5 plates) Infectious Laryngotracheitis Antibody test kit, solid plate format The Infectious Laryngotracheitis Antibody test kit will measure the amount of antibodies to the Gallid herpesvirus 1. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. Mg ELISA USDA LICENSED Catalogue number: CK114 Tests/kit: 480 tests (5 plates) Mycoplasma gallisepticum Antibody test kit, solid plate format The Mycoplasma gallisepticum Antibody test kit will measure the amount of antibodies to Mycoplasma gallisepticum. The test will determine the presence of antibodies in the serum of chickens and turkeys. Serum dilution is 1:500. Test incubation is minutes. Tests read at 405 nm wavelength. Ms ELISA USDA LICENSED Catalogue number: CK115 Tests/kit: 480 tests (5 plates) Mycoplasma synoviae Antibody test kit, solid plate format The Mycoplasma synoviae Antibody test kit will measure the amount of antibodies to Mycoplasma synoviae. The test will determine the presence of antibodies in the serum of chickens and turkeys. Serum dilution is 1:500. Test incubation is minutes. Test read at 405 nm wavelength. Mg/Ms ELISA USDA LICENSED Catalogue number: CK215 Tests/kit: 480 tests (5 plates) Mycoplasma gallisepticum - synoviae Antibody test kit, solid plate format The Mycoplasma gallisepticum -synoviae Antibody test kit will measure the amount of antibodies to Mycoplasma gallisepticum and Mycoplasma synoviae. The test will determine the presence of antibodies in the serum of chickens and turkeys. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. Mm ELISA Catalogue number: CK109 Tests/kit: 480 tests (5 plates) Mycoplasma meleagridis Antibody test kit, solid plate format The Mycoplasma meleagridis Antibody test kit will measure the amount of antibodies to Mycoplasma meleagridis. The test will determine the presence of antibodies in the serum of turkeys. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength

25 ELISA TEST KITS - POULTRY NDV ELISA USDA LICENSED Catalogue number: CK116 Tests/kit: 480 tests (5 plates) Newcastle Disease Virus Antibody test kit, solid plate format The Newcastle Disease Virus Antibody test kit will measure the amount of antibodies to the Newcastle Disease Virus (Avian Paramyxovirus type 1). The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. NDV-F ELISA Catalogue number: CK122 Tests/kit: 480 tests (5 plates) Newcastle Disease Virus Antibody test kit, solid plate format The Newcastle Disease Virus Antibody test kit, will measure the amount of ND vector vaccine induced antibodies to the Newcastle Disease Virus (Avian Paramyxovirus type 1). The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. ORT ELISA Catalogue number: CK108 Tests/kit: 480 tests (5 plates) Ornithobacterium rhinotracheale Antibody test kit, solid plate format The Ornithobacterium rhinotracheale Antibody test kit will measure the amount of antibodies to Ornithobacterium rhinotracheale in the serum of chickens and turkeys. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. REO ELISA USDA LICENSED Catalogue number: CK110 Tests/kit: 480 tests (5 plates) Avian Reovirus Antibody test kit, solid plate format The Avian Reovirus Antibody test kit will measure the amount of antibodies to the Avian Reovirus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:500. Test incubation is minutes. Test reads at 405 nm wavelength. REV ELISA Catalogue number: CK125 Tests/kit: 480 tests (5 plates) Reticuloendotheliosis Virus Antibody test kit,solid plate format The Reticuloendotheliosis Virus Antibody test kit will measure the amount of antibodies to the Reticuloendotheliosis Virus. The test will determine the presence of antibodies in the serum of chickens. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. 25

26 ELISA TEST KITS - POULTRY Salm Gp D ELISA Catalogue number: CK117 Tests/kit: 480 tests (5 plates) Salmonella Group D Antibody test kit, solid plate format The Salmonella Group D Antibody test kit will measure the amount of antibodies to Salmonella enteritidis and other invasive group D Salmonella species (Salmonella pullorum, Salmonella gallinarum) in the serum of chickens and turkeys. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. Salm Gp B ELISA Catalogue number: CK118 Tests/kit: 480 tests (5 plates) Salmonella Group B Antibody test kit, solid plate format The Salmonella Group B Antibody test kit will measure the amount of antibodies to Salmonella typhimurium and other invasive group B Salmonella species in the serum of chickens and turkeys. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. Salm Gp B/D ELISA USDA LICENSED Catalogue number: CK218 Tests/kit: 480 tests (5 plates) Salmonella Group B and D Antibody test kit, solid plate format The Salmonella Group B and D Antibody test kit will measure the amount of antibodies to Salmonella enteritidis and Salmonella typhimurium, and other invasive group B and D Salmonella species, in the serum of chickens. Serum dilution is 1:100. Test incubation is minutes. Test reads at 405 nm wavelength. Thanks to the BioChek ELISA test kits, software and data management systems, we can consult the test results quickly, have confidence in their accuracy and most importantly gain rapid insight into the effectiveness of our vaccination programs. Eric L. Jensen, DVM, MAM, dacpv Vice President of Veterinary Services, Aviagen North America 26 26

27 ELISA REFERENCE CONTROLS & ACCESSORIES - POULTRY Pre-diluted Reference Control Catalogue number: CR100 Tests/kit: 5 ml / vial The Pre-diluted Reference Control is a positive control with known antibody titers for IBD, IBV, NDV, ART, REO, AE, CAV, Mg, Ms, Mg/Ms, EDS, Salmonella group D (SE), Salmonella group B (ST), Salmonella group B and D (SE/ST), AI, ILT, FAdV, ORT and BLS. By using this reference control, all steps of the analysis are checked, except for the dilution step. Freeze Dried Reference Serum Catalogue number: CR300 Tests/kit: 200 μl / vial The Freeze Dried Reference Serum CR300 contains significant and known antibody levels to NDV, IBV, IBD, AE, ART, CAV, FAdV and REO. By using this reference serum, all steps of the analysis, including the dilution step, are checked. Freeze Dried Negative Reference Serum Catalogue number: CR400 Tests/kit: 200 μl / vial The Freeze Dried Negative Reference Serum CR400 is a negative control serum for all BioChek Poultry ELISA Test Kits. This reference serum will allow customers to conduct their own continuous quality control. ALV Pre-diluted Reference Control Catalogue number: CR200 Tests/kit: 2.5ml / vial The ALV Pre-diluted Reference Control is a positive control for Lymphoid Leukosis. The reference control contains significant and known antigen levels of p27. By using this reference control, all steps of the analysis are checked, except for the dilution step. BioChek Partial Plate kit set Catalogue number: BC PP The Partial Plate kit set contains all the necessary instructions and materials (plate sealers, sealable storage pouches, desiccant pouches) for running and storing partial test plates. The correct use of the BC PP kit set, prevents the deterioration of coated antigen plates, extending the storage life of partially used test plates to 2-6 weeks after first use. 27

28 PCR TEST KITS - POULTRY The BioChek quantitative Polymerase Chain Reaction (PCR) Test Kits will detect the presence of target specific DNA/RNA. Every PCR test kit uses the same thermocycler protocol thus enabling the technician to perform PCR reactions for different targets at the same time on 1 thermocycler. All BioChek PCR kits are validated for use with a variety of thermocyclers (For a complete list of compatible PCR thermocyclers please contact BioChek) and a total NA extraction kit. Results are given in Cq values, allowing differentiation between low, medium and high loads of antigen. All necessary controls are included (Negative Control, Positive Control and Internal Control). ILT PCR Catalogue number: CP104 Tests/kit: 100 tests Fowl Laryngotracheitis DNA Test Kit The Fowl Laryngotracheitis DNA Test Kit allows for the detection of the Gallid herpesvirus 1. The test will determine the presence and load of antigen in the trachea of chickens. Test Duration after DNA extraction is approximately 90 minutes. Test reads at Fam and CY5 (Quasar 670) channels. For quantification of results PCR standards ILT are available. Mg-Ms PCR Catalogue number: CP101 Tests/kit: 100 tests Mycoplasma Gallisepticum-Synoviae DNA Test Kit The Mycoplasma Gallisepticum-Synoviae DNA Test Kit allows for the simultaneous detection of Mycoplasma gallisepticum and/or Mycoplasma synoviae. The test will determine the presence and load of antigen in the trachea of chickens or turkeys. Test duration after DNA extraction is approximately 90 minutes. Test reads at FAM, HEX (VIC) and CY5 (Quasar 670) channels. For quantification of results, PCR standards Mg-Ms are available. NDV PCR Catalogue number: CP102 Tests/kit: 100 tests Newcastle Disease Virus RNA Test Kit The Newcastle Disease Virus RNA Test Kit allows for the simultaneous detection of the Newcastle disease virus and for a virulence factor (Mesogenic or velogenic marker) in extracts from tracheal/chloacal swabs in poultry. Test duration after RNA extraction is approximately 90 minutes. Test reads at FAM (whole NDV), Texas Red (meso/velo) and CY5 (IC) channels. For quantification of results, PCR standards NDV are available

29 ORT PCR Catalogue number: CP105 Tests/kit: 100 tests Ornithobacterium rhinotracheale DNA Test Kit The Ornithobacterium rhinotracheale DNA Test Kit allows for the detection of Ornithobacterium rhinotracheale. The test will determine the presence and load of antigen in the trachea of chickens or turkeys. Test Duration after DNA extraction is approximately 90 minutes. Test reads at FAM and CY5 (Quasar 670) channels. For quantification of results PCR standards ORT are available. Salmonella PCR Catalogue number: MP104 Tests/kit: 100 tests Salmonella Species DNA Test Kit The Salmonella Species DNA Test Kit allows for the detection of all Salmonella species. The test will determine the presence and load of antigen in faecal samples, meat samples or dust samples after pre-enrichment in suitable media. Test duration after DNA extraction and enrichment is approximately 90 minutes. Test reads at Fam and HEX channels. For quantification of results, PCR standards Salmonella are available. Spp-Se-St PCR Catalogue number: MP102 Tests/kit: 100 tests Salmonella Species-Enteritidis-Typhimurium DNA Test Kit The Salmonella Species-Enteritidis-Typhimurium DNA Test Kit allows for the simultaneous detection of Salmonella spp, Salmonella enteritidis and Salmonella typhimurium. The test will determine the presence and load of antigen in faecal samples, meat samples or dust samples after pre-enrichment in suitable media. Test duration after DNA extraction and enrichment is approximately 90 minutes. Test reads at Fam, CY5 (Quasar 670) channels, HEX and Texas Red channels. For quantification of results, PCR standards Salm are available. Se-St-Sh PCR Catalogue number: CP107 Tests/kit: 100 tests Salmonella Enteritidis-Typhimurium-Heidelberg DNA Test Kit The Salmonella Enteritidis-Typhimurium-Heidelberg DNA Test Kit is a complete set of reagents which allows for the simultaneous detection of Salmonella Enteritidis-Typhimurium-Heidelberg DNA Test Kit. The test will determine the presence and load of antigen in faecal samples, meat samples or dust samples. Test duration after DNA extraction and enrichment is approximately 90 minutes. Test reads at Fam, CY5 (Quasar 670), HEX and Texas Red channels. For quantification of results, PCR standards Se-St-Sh are available. 29

30 PCR TEST KITS - POULTRY Campy PCR Catalogue number: MP103 Tests/kit: 100 tests Campylobacter Coli-Jejuni-Lari DNA Test Kit The Campylobacter Coli-Jejuni-Lari DNA Test Kit allows for the simultaneous detection of Campylobacter coli, Campylobacter jejuni and Campylobacter lari. Test duration after DNA extraction is approximately 90 minutes. Test reads at Fam, CY5 (Quasar 670), HEX and Texas Red channels. For quantification of results, PCR standards Campy are available. InfA PCR Catalogue number: MP101 Tests/kit: 100 tests Influenza A RNA Test Kit The Influenza A RNA Test Kit allows for the detection of Influenza A. The test will determine the presence and load of antigen in tissues, swabs or (oral) fluids of any type of animal. Test duration after RNA extraction is approximately 100 minutes. Test reads at Fam and CY5 (Quasar 670) channels. For quantification of results, PCR standards InfA are available. Just like the products, the service and support are first rate Jennifer O Brien & Anna O Connor Laboratory/Quality Manager & PCR Manager, St David s Laboratory Services Ltd 30 30

31 PCR STANDARDS & ACCESSORIES - POULTRY PCR standards ILT Catalogue number: CP204 4 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Fowl Laryngotracheitis DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards InfA Catalogue number: MP201 Tests/kit: 4 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Influenza A RNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Mg/Ms Catalogue number: CP201 Tests/kit: 8 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Mycoplasma Gallisepticum-Synoviae DNA Test Kit. The standards are ready for use and do not need to be extracted.these standards can be used to check performance of the PCR or to quantify results. PCR standards NDV Catalogue number: CP202 Tests/kit: 8 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Newcastle Disease Virus RNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards ORT Catalogue number: CP205 4 vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Ornithobacterium rhinotracheale DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. 31

32 PCR STANDARDS & ACCESSORIES - POULTRY PCR standards Salmonella Catalogue number: MP204 Tests/kit: 12 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Salmonella Species DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Spp-Se-St Catalogue number: MP202 Tests/kit: 12 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Salmonella Species-Enteritidis-Typhimurium DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Se-St-Sh Catalogue number: CP vials (12 reactions per vial) A set of PCR standards can be ordered separately for the Salmonella Enteritidis-Typhimurium-Heidelberg DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards Campy Catalogue number: MP203 Tests/kit: 12 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Campylobacter Coli-Jejuni-Lari DNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results. PCR standards InfA Product Code: MP201 Tests/kit: 4 vials (12 reactions per vial) A set of PCR standards can be ordered seperately for the Influenza A RNA Test Kit. The standards are ready for use and do not need to be extracted. These standards can be used to check the performance of the PCR and to quantify results

33 PCR STANDARDS & ACCESSORIES - POULTRY Nucleic Acid Extraction Kit Catalogue number: BP801 Tests/kit: 100 extractions The Nucleic Acid Extraction Kit is a complete set of chemical reagents which allows for the extraction and purification of Nucleic Acid from a variety of samples (serum, swabs, oral fluids, tissue etc.).the amount of eluted Nucleic Acid after the process is 50 μl. The Total Nucleic Acid Extraction kit can be used with all BioChek PCR kits and can be stored at room temperature. In order to use the kit, 96% ethanol must be ordered separately. Lysis Buffer A Catalogue number: BP900 2 reagents (100 reactions) Lysis Buffer A is a complete set of reagents to perform DNA extraction (not suitable for RNA extraction). The only equipment that is needed is a heating block. The buffer is validated for Mg-Ms and Salmonella extraction. Check with the technical staff from BioChek for which other targets and matrices it can be used and for further specific details. 33

34 PRODUCT CATALOGUE EQUIPMENT 34 34

35 EQUIPMENT BEAR Catalogue number: GSD00300 The BEAR (BioChek ELISA Assay Robot) The BEAR is a complete solution for running all your BioChek ELISAs. Load the BEAR with kit components and serum samples, press start and walk away. Results will be available in 2-4 hours. The results will be stored and can be accessed by the BioChek Software. Automation Platform: Fully Automated Load and walk away High capacity Runs up to 6 plates a day High precision pipette 1 µl with less than 3% CV Robust Software BioChek interface, full log of all steps Footprint 60 cm x 60 cm with 28 kg weight hold Green and economical no pipette tips Want to know more? Go to biochek.com/bear Contact your local BioChek representative for further information on equipment not included in the catalogue. 35

36 PRODUCT CATALOGUE SOFTWARE 36 36

37 SOFTWARE BioChek II Software Swine and Poultry BioChek II Software (for Windows) including App (for Android and ios) The BioChek Diagnostic Software is a comprehensive package for managing your database and gives you 24/7 access to all results, data and trend reports. BioChek provides customers with access to the BioChek Diagnostics Software for the analysis of PCR and ELISA diagnostic tests. The BioChek Software is divided into two different parts: one designed for the laboratory and one for the field user (veterinarian, flock supervisor or farm owner). The software package consists of a database management program to be used by the laboratory, an online database and an app for field users. The BioChek app is available for Android and ios. For more information about BioChek Software or to request the BioChek Software, please visit our website: The BioChek II Software makes it easier for me Phil Stayer, DVM Corporate Veterinarian for Sanderson Farms 37

38 PRODUCT CATALOGUE LABTRAINING 38 38

39 LABTRAINING Labtraining Swine and Poultry Join our Lab Trainings and learn more about ELISA and PCR! BioChek frequently organizes open Lab Trainings for ELISA and/or PCR. The BioChek Lab training is meant for laboratory technicians using BioChek ELISA and/or PCR kits. Contact us for more information on training opportunities, or for the planned dates of a training in your region! What you ll focus on during a BioChek Lab Training: Pipetting technique Accuracy of results Equipment maintenance The use of the BioChek II software (both ELISA and PCR) Do you want more information? Check or mail us info@biochek.com 39

40 Welcome to the World of BioChek The complete BioChek package for veterinary diagnostics of poultry and swine diseases is used virtually all over the world. With its head office in the Netherlands, an R&D department and production facility in the United Kingdom, a USDA licensed facility in America and numerous distributors, BioChek is able to assist the poultry and swine industries in perfecting health and improving welfare and productivity of animals. Would you like more information on BioChek or to work together on animal health? Then please contact us. After all, reliable veterinary diagnostics start with BioChek! For more information, please visit our website: Fokkerstraat ER Reeuwijk the Netherlands Tel.: +31 (0) Fax: +31 (0) info@biochek.com BioChek USA Scarborough 3 Southgate Road Scarborough, ME United States Tel.: +01 (207) timgoode@biochek.com 40 40