Biology (with a concentration in Biochemistry and Cellular and Molecular Biology) University of Tennessee, Knoxville Awarded in May 2001

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1 Business Address: 101-A Science & Engineering Bldg University of Tennessee Knoxville, TN Business Phone: (865) Steven C. Minkin Jr. Home address: 6506 Westland Dr. Knoxville, TN Phone : (865) sminkin@utk.edu Education Bachelor of Science Doctor of Philosophy Biology (with a concentration in Biochemistry and Cellular and Molecular Biology) Awarded in May 2001 Microbiology Awarded in August 2008 Laboratory Experience Post-doctoral Research (9/08-present) PI: John P. Biggerstaff, CEB Biocompatability testing, Environmental microbiology, Advanced microscopy Graduate Student Research (4/02-8/08) PI: Jeffrey M. Becker, Micro. Dept. Dissertation: Post-translational regulation of a peptide transport protein in S.cerevisiae Laboratory Technician BCN Research Labs, Knoxville, TN (5/01-8/01) (Food microbiology, FDA) Primary duties consisted of media preparation, sample preparation, culture, and analysis. Undergraduate research assistant (5/00-12/00) PI: Robert Moore, Micro. Dept. Project: Antibody-fragment (ScFv) based therapy for pneumonic pasteurellosis

2 Undergraduate research assistant (1/99-6/99) PI: Naima Moustaid-Moussa, Nutrition Dept. Project: Effects of Angiotensin II on the Fatty Acid Synthase (FAS) gene promoter Teaching Experience () -Eleven semesters of teaching experience including Advanced Cell Biology 411 (majors), Cell Biology Lab 140 (majors), Microbiology Lab 210 (non-majors), Microbiology Lab 319 (majors), and Advanced Microbiology Lab 329 (majors). Advanced Cell Biology 411 covers cellular structure and function at the molecular and supramolecular level. Topics include protein structure and function, membrane structure and function, signal transduction and cell regulation, mitosis and the cell cycle, cytoskeleton and cell motility, cell-cell interactions and tissues (co-instructor, 1- semester). Advanced Microbiology 329 Lab provides an intensive coverage of topics and techniques regularly used in molecular biology (emphasis on nucleic acid work: restriction digests, ligation, electrophoresis, PCR, Southern blot, sequencing, transformation, BLAST, etc.) (2 semesters) Microbiology 210 and 319 Labs are designed to teach students general properties of microorganisms including physiology, metabolism, genetics, and pathogenesis as well as immunity and applied microbiology. These courses also teach methods for examination, cultivation, and identification of microorganisms. (3 and 4 semesters respectively) Cell Biology 140 Lab is designed to teach students basic laboratory skills and procedures such as measuring, pipetting, and mixing solutions, as well as introduce modern methods for analysis of cell components such as electrophoresis and centrifugation. (1 semester) Research Skills Bench work has involved: Routine microbial practices: cell culture, media prep, sterilization, light microscopy, microbial quantification, cultivation, and identification. Molecular techniques: DNA isolation, genetic transformation of E. coli and S. cerevisiae, DNA restriction analysis, PCR, site-directed mutagenesis, in vivo and in vitro ligations, subcloning, gel electrophoresis including agarose and PAGE, Western blotting, Southern blotting, Northern blotting, RNA isolation, LacZ reporter assays, metabolic labeling ( 32 P), and protein purification/enrichment. Advanced microscopic techniques: fluorescence microscopy, confocal laser-scanning microscopy, TIRF, deconvolution, 3D and time-lapse imaging. Other techniques: Peptide growth assays, toxic peptide halo-assays, membrane enrichment, subcellular fractionation, equilibrium density gradient centrifugation, flowcytometry, and peptide transport assays involving radiolabeled substrates. Computer work has involved:

3 Extensive use of computers for image analysis and nucleotide/protein sequence analysis. Commonly used software packages include: DNASTAR, PRISM, Quantity One (Bio- Rad), NIS-Elements (Nikon), AutoQuant/AutoDeblur, and Microsoft Office Presentations 2009 International Society for Thrombosis and Haemostasis (ISTH) Boston, MA (Poster) 2007 Southeastern Regional Yeast Meeting (SERYM) UAB, Birmingham, AL (Poster) 2005 Southeastern Regional Yeast Meeting (SERYM) Georgia Tech, Atlanta, GA (Poster) 2005 Genome Science and Technology (GST) Retreat Knoxville, TN 2004 Southeastern Regional Yeast Meeting (SERYM) Rhodes College, Memphis, TN 2004 Genome Science and Technology (GST) Retreat The Foundry, Knoxville, TN (Poster) (Talk) (Poster) Community Outreach Education 2007 KidsU Microbiology, UTK 8 th -12 th grade (5 days) 2005 Gibbs Elementary Earth Science Day 3 rd and 4 th grade (1 day) 2005 SHADES (Sharing Adventures in Engineering and Science) 6 th and 7 th grade (1 day)

4 Other Radiation Safety Officer (for the Becker Lab) Duties include: Monthly radionuclide accounting, waste disposal, ensuring laboratory compliance with state and federal radioisotope regulations. President, Microbiology Graduate Student Association (MSGA) Activities included: Organizing departmental tutoring, MGSA fund-raising, coordinating social events, etc. Graduate Student Senate (Member 2005, 2006) Representing the Dept. of Microbiology in the GSS References John P. Biggerstaff, Ph.D. Research Professor Center for Environmental Biotechnology 232-D Science and Engineering Building Phone: Jeffrey M. Becker, Ph.D. Professor and Head of Microbiology Phone: Melinda R. Hauser, Ph.D. Research Assistant Professor Phone:

5 Todd Reynolds Ph.D. Assistant Professor of Microbiology Phone: Barry Bruce Ph.D. Professor, Department of Biochemistry, Cellular & Molecular Biology Department of Biochemistry, Cellular & Molecular Biology E404 Walters Life Sciences Knoxville, TN Phone: Elizabeth F. McPherson Special Instructor (Director of Undergraduate Microbiology Teaching Labs) Phone: