One just right for you.

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1 amplification One just right for you. Whether you have 10 samples or 10,000 discover your perfect match in the full range of Bio-Rad PCR consumables. Precise manufacturing ensures optimal fit in our thermal cyclers Optical plates and seals give dependable real-time results Hard-Shell warp-free plates allow reliable laboratory automation Tight-sealing vessels prevent sample loss Products tested to be negative for DNase, RNase, and DNA contamination Dedicated technical support provided by experienced scientists For more information, visit our gateway to genomics applications at Bio-Rad offers validated consumables for the MJ Mini cycler, the 4-bay DNA Engine Tetrad 2 cycler, and everything in between. Notice regarding Bio-Rad thermal cyclers and real-time systems. Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser s own internal research and development and for use in applied fields other than Human In Vitro Diagnostics under one or more of U.S. Patents Nos. 5,656,493, 5,333,675, 5,475,610 (claims 1, 44, 158, and 167 only), and 6,703,236 (claims 1 7 only), or corresponding claims in their non-u.s. counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Bio-Rad s real-time thermal cyclers are licensed real-time thermal cyclers under Applera s United States Patent No. 6,814,934 B1 for use in research and for all other fields except the fields of human diagnostics and veterinary diagnostics. Visit us on the Web at discover.bio-rad.com Call toll free at BIORAD ( ); outside the US, contact your local sales office.

2 Subject Coverage Antibodies Bioinformatics/Genomics Cell Biology Chromatography Computational Biology DNA Delivery/Gene Transfer Electrophoresis Genetics High Throughput Analysis Imaging/Microscopy Immunology Laboratory Organisms Molecular Biology Neuroscience Plant Biology Polymerase Chain Reaction (PCR) Proteins and Proteomics RNA Interference (RNAi)/siRNA Now you can see results of the finest methods in action with new online movies in CSH Protocols. The November issue of CSH Protocols features methods for tracking RNA and protein molecules in both cells and organisms, and introduces new multimedia content made possible by online presentation: movies showing examples of these techniques in action. Read and see these newly featured protocols online at Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila CSH Protocols offers cutting edge and classic protocols in molecular and cell biology from laboratories worldwide, along with methods from Cold Spring Harbor Laboratory s renowned manuals and on-site courses. Free trials and discounted subscriptions are available for a limited time. CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with HighWire Press of Stanford University. Cold Spring Harbor Protocols Executive Editor: Dr. Michael Ronemus ISSN / online, monthly Available exclusively via institutional site license Stem Cells Transgenic Technology For pricing information or to request a free trial visit the website or: Phone: (Continental US and Canada) or (all other locations) FAX: cshpress@cshl.edu Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY

3 Genes & Development G & Dee v e lno p m ee n t s Volume 20 No.23 December 1, 2006 A JOURNAL OF CELLULAR AND MOLECULAR BIOLOGY 20(23): G&D December 1, 2006 Cold Spring Harbor Laboratory Press

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5 Postdoctoral Fellow Position Department of Biology University of Washington at Seattle I am looking for a highly motivated Postdoctoral Fellow to study RNA localization in a giant unicell, Acetabularia acetabulum. We have identified 3 conserved domains in our EST libraries whose functions we would like to understand. In specific, we would like to test if they act like "zipcodes" to tell mrnas where to go in the organism, or as stability signals, etc. Microinjection studies will determine 1) what happens when these conserved elements are altered in order, number etc.; 2) on which portion of the cytoskeleton do fluorescent beads or fluorescently tagged RNAs travel during development with and without these consensus regions; 3) whether there are some conserved sequences that direct an mrna to the apex or base of the organism; 4) the role of the nucleus (if any) in directing RNA trafficking. Imaging with confocal and/ or epifluorescence video microscopy and fluorescence in situ hybridizations will be the main assays. The successful candidate will perform the microinjections, analyze the fluorescence microscopy data and will work closely with a technician who will assist in making the molecular constructs. This work will be complemented by searches for localized, stable transcripts of A. acetabulum ESTs or genes. The successful candidate will be expected to apply for independent postdoctoral fellowship(s) during this 2 year NSF postdoctoral period. Start date for this position is immediate. Contact : Dr. Dina F Mandoli Tel : (206) mandoli@u.washington.edu

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