Fluorescence In Situ Hybridization (FISH) - Application Guide

Transcription

1 Fluorescence In Situ Hybridization (FISH) - Application Guide Bearbeitet von Thomas Liehr 1. Auflage Buch. xviii, 451 S. Hardcover ISBN Format (B x L): 15,5 x 23,5 cm Gewicht: 951 g Weitere Fachgebiete > Medizin > Vorklinische Medizin: Grundlagenfächer > Physiologie, Pathophysiologie Zu Leseprobe schnell und portofrei erhältlich bei Die Online-Fachbuchhandlung beck-shop.de ist spezialisiert auf Fachbücher, insbesondere Recht, Steuern und Wirtschaft. Im Sortiment finden Sie alle Medien (Bücher, Zeitschriften, CDs, ebooks, etc.) aller Verlage. Ergänzt wird das Programm durch Services wie Neuerscheinungsdienst oder Zusammenstellungen von Büchern zu Sonderpreisen. Der Shop führt mehr als 8 Millionen Produkte.

2 Contents Part I Molecular Cytogenetics, Probes and Equipment 1 Molecular Cytogenetic Applications in Diagnostics and Research: An Overview... 3 Nedime Serakinci and Steen Kølvraa 2 Molecular Cytogenetics: The Standard FISH and PRINS Procedure Thomas Liehr and Franck Pellestor 3 Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, and Nigel P. Carter 4 ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka, and Thomas Liehr 5 PNA-FISH Technique for In Situ Assessment of Aneuploidy Franck Pellestor, Petra Paulasova, and Samir Hamamah 6 Oligonucleotide FISH Probes Sean Bradley, Leah Zamechek, and Joan Aurich-Costa 7 Microscopy and Imaging Systems Ivan Iourov vii

3 viii Contents 8 Optical Filters for FISH Michael Sommerauer and Ingrid Feuerbacher 9 FISH With and Without COT1 DNA Vladimir A. Trifonov, Nadezhda N. Vorobieva, and Willem Rens Part II FISH Applications in Different Tissue Preparations 10 Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts Anja Weise and Thomas Liehr 11 Prenatal Diagnostics on Uncultured Amniocytes Bernd Eiben, Ralf Glaubitz, and Hans-Jürgen Arens 12 Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan, and Thomas Liehr 13 Application of FISH to Archival Cytogenetic Slides Thomas Liehr and Erich Gebhart 14 Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, and Thomas Liehr 15 Characterization of Archived Formalin-Fixed/ Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction Thomas Liehr and Marina Manvelyan 16 FISH on Sperms, Spermatocytes and Oocytes Maria Oliver-Bonet Part III Characterization of Marker Chromosomes 17 Multiplex FISH and Spectral Karyotyping Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko, and Lukrecija Brecevic

4 Contents ix 18 Tips and Tricks for mfish Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, and Roderick A.F. MacLeod 19 Simultaneous Fluorescence Immunostaining and FISH Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye, and Henry H.Q. Heng 20 Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative Octavian Henegariu 21 Three-Color FISH for the Detection of Individual Radiosensitivity Luitpold Distel, Ulrike Keller, and Susann Neubauer 22 FISH Banding Techniques Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Heike Nelle, Vladimir Trifonov, Marina Manvelyan, and Anja Weise 23 cenm-fish Approaches Nadezda Kosyakova, Anja Weise, Kristin Mrasek, Hasmik Mkrtchyan, Vivien Klaschka, Elisabeth Ewers, Daniela Reich, Nadine Rüdiger, and Thomas Liehr Part IV Characterization of Cryptic and Epigenetic Aberrations 24 Subtelomeric and/or Subcentromeric Probe Sets Anja Weise, Kristin Mrasek, Madeleine Gross, Vivien Klaschka, and Thomas Liehr 25 Quantitative DNA Fiber Mapping Chun-Mei Lu, Mei Wang, Karin M. Greulich-Bode, Jingly F. Weier, and Heinz-Ulli G. Weier 26 Parental Origin Determination FISH: Pod-FISH Anja Weise, Kristin Mrasek, Hasmik Mkrtchyan, Madeleine Gross, Sophie Hinreiner, and Thomas Liehr

5 x Contents Part V Interphase FISH and Characterization of the Nuclear Architecture 27 Interphase FISH: Detection of Intercellular Genomic Variations and Somatic Chromosomal Mosaicism Ivan Y. Iourov, Svetlana G. Vorsanova, Ilia V. Soloviev, and Yuri B. Yurov 28 Three-Dimensional Interphase Analysis Enabled by Suspension FISH Friederike Hunstig, Marina Manvelyan, Samarth Bhatt, Ulf Steinhaeuser, and Thomas Liehr Part VI Applications of FISH in Zoology, Botany and Microbiology 29 Animal Probes and ZOO-FISH Fengtang Yang and Alexander S. Graphodatsky 30 FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast Harry Scherthan 31 FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach Thomas Liehr 32 FISH on Plant Chromosomes Peng Zhang and Bernd Friebe 33 FISH in Food Microbiology Benedetta Bottari, Danilo Ercolini, Monica Gatti, and Erasmo Neviani Part VII Comparative Genomic Hybridization (CGH) and Array CGH 34 Micro-CGH: Microdissection-Based Comparative Genomic Hybridization Anita Heller and Thomas Liehr 35 Array CGH: Opening New Horizons Joris R. Vermeesch Part VIII Internet Links for FISH 36 FISH and Chips on the Internet Thomas Liehr and Anja Weise Index