ADSC Animal Biotechnology Syllabus Fall 2011

Transcription

1 ADSC Animal Biotechnology Syllabus Fall 2011 Instructor: Dr. Franklin West 425 River Road Room 308 Athens, GA Phone: Website: Course Objective: The objective of this course is to introduce students to cutting edge biotechnologies that can be used for animal and human health and research. In this course we will analyze and discuss the primary literature on stem cells, cloning, large animal models for disease and development of therapies and treatments. This class will cover basic cellular and molecular biology techniques and their applications in a real world research setting. Manuscripts: This class is meant to be a cutting edge course of discussion and analysis of current technologies. To this end, the class will rely heavily on the primary literature. Manuscripts will be provided on the West Laboratory Website ( Manuscripts for class can be retrieved as PDFs or the like underneath the tab UGA courses and then by clicking ADSC3130. The web page is password protected and the instructor will provide the password. Notes: Each student is expected to take their own notes and to attend class. If you choose not to attend, all missed handouts and announcements are your responsibility and will not be redistributed. You must get any handouts or notes from your classmates. You are also expected to be on time, and if you arrive late, you may get the notes after class, not during, so as not to disrupt the class flow. Exams (100 Points Each): Exams will consist mainly of multiple choice, fill in the blank, and true/false questions. There will be no make-up exams without an excused absence, which is severe illness or death in the family. Dr. West must be notified within 24 hours of a missed test. If no contact is made within 24 hours no make-up will be given. Notes from doctors must be dated the day of the missed exam and/or must state that the medical condition prevented the student from taking the test. An alternative test will be given to students that missed the exam. Exam I Sept 14 Exam 2 Nov 9 Mid-Term Paper (100Points): The mid-term paper is on the topic of your choice that covers anything under the purview of animal biotechnology (cloning, stem cells, genetic engineering, extracellular matrices, etc.). The mid-term paper is to be a single spaced 6 to 7 page document with 1 inch margins with writing in 11 to 12 point Times New Roman font. This document should include:

2 I. Introduction: Typically ¾ to1 page 20 Points a. Relevance and Significance of Subject b. Aim of Review II. Review of the Literature: 40 Points a. Previous Findings b. Real Life Applications c. Future Direction of the Field d. Use sub-headings to organize, but be original in your sub-headings and don t just use the ones provided here. III. Conclusion: Typically ½ to ¾ page 10 Points a. Summarization of findings and future potential. IV. Citations: Using EndNote Numbered Style 10 Points V. Figures and Tables: 2 to 3 figure that occupy a max. of 25% of a single page 20 Points a. Can include graphs, illustrations, diagrams, pictures and tables. b. Should include legend and be incorporated into document. Total 100 Points Deductions: Deductions include but are not limited to: not following the rules on margins, page limit, font size or type will result in a 10 point deduction for each infraction. Two points will be deducted for every 5 spelling and grammatical errors. Content will also be critically judged for completeness, relevance and logical organization. Plagiarism WILL result in a zero without exception. The Mid-Term Paper will be due on October 3 rd and will be turned in during the class period. The mid-term will not be accepted late. If you are unable to turn the paper in on the day that it is due (October 3 rd), it should be handed in prior. If the mid-term is not turned in on time, the student will receive a score of zero for the assignment. Presentation (100 Points): Presentations will be on a paper of the students choosing that is under the umbrella of biotechnology. A day to present will be assigned to each student and if the student is unable to present the professor is to be informed at least 72 hours in advance and the student will be assigned another day. Rescheduling of presentation will require an excuse and missing a presentation without an excuse will result in a score of zero for the activity. Papers to be presented must be sent to the professor in PDF format by (westf@uga.edu) a minimum of 7 days before it is presented in class to be approved and distributed to others in the class. The paper must be no more than 2 years old and contain at least 4 figures that are included in the main body of the manuscript (does not include supplementary materials), unless the manuscript is from the journals Nature, Science or PNAS. The presenter will be judged on: I. Aesthetically Pleasing: 20 Points 1. Presentation showed color coordination 2. Headings and font were similar or identical

3 3. Lacked spelling and grammar errors II. Organization: 55 Points 1. Did the presenter give background on topic 2. Did the presenter identify the main question and hypothesis of paper and sub- questions and hypotheses 3. Did the presenter describe experimental design 4. Did the presenter describe results 5. Did the presenter describe conclusions III. Understanding: 30 Points 1. Did the presenter pose interesting questions to the class 2. Did the presenter adequately answer questions Total 100 Points Labs (35 Points Each): There will be 3 labs for this class where students will be exposed to PCR, immunocytochemistry and flow cytometry techniques. The class will be split into two or three sections with students being assigned to a specific section. Student attendance is compulsory for participation grade. If a student cannot attend, they must inform the professor 24hrs before the section and attend another section. There are no makeup labs and a student that does not come to lab will receive a zero for the work. Questions from these labs will likely be on the test. Attendance (50 Points): Attendance will be taken daily. After the first two absences the student will be deducted 5 points per absence without it being an excused absence, which is severe illness or death in the family. Grading: Exams (2): 100 Points Each Mid-Term Paper: 100 Points Presentation: 100 Points Labs: 100 Points (35 Points Each) Attendance: 50 Points Total Possible Points: 550 (There is a 5 point cushion built in so the real possible total is 555). Grades are calculated by dividing the total number of points earned by the total of points possible and multiplying by 100. Scores will be rounded up if >0.5 and down if< 0.5. Example: (500/550)*100= 91= A. A+= A= A- = B+ = B= B-= C+= C= C-= D= F= 59-0

4 Academic Honesty: As a University of Georgia student, you have agreed to abide by the University s academic honesty policy, A Culture of Honesty, and the Student Honor Code. All academic work must meet the standards described in A Culture of Honesty found at: Lack of knowledge of the academic honesty policy is not a reasonable explanation for a violation. Questions related to course assignments and the Academic Honesty policy should be directed to the instructor. Technology: The use and the presence of cell phones during class is strictly prohibited. The presence of a cell phone and other electronic devices (e.g. ipad, MP3 player) during a test will result in the picking up of your test and your dismissal from class. Final Note: The course syllabus is a general plan for the course; deviations announced to the class may be necessary.

5 August 15 Introduction to Animal Biotechnology August 17 Adult Stem Cells In Clinical Trials August 19 The Discovery of Embryonic Stem Cells August 22 Embryonic Stem Cells in Action August 24 Reprogramming Somatic Cells into Induced Pluripotent Stem Cells August 26 Wallowing in Induced Pluripotent Stem Cells August 29 Stem Cells in Domestic Live Stock Species (Dr. Helms-Boone) August 31 Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes September 2 Student Selected Paper and Discussion September 5 Labor Day No Class Today September 7 Cell Culture Experience: Fibroblast, Adult and Pluripotent Stem Cells (Group A) September 9 Student Selected Paper and Discussion September 12 Cell Culture Experience: Fibroblast, Adult and Pluripotent Stem Cells (Group B) September 14 Direct Reprogramming of Cells to Beta Islet Cells September 16 Student Selected Paper and Discussion September 19 Ethics of Human Stem Cells September 21 Test 1 September 23 Student Selected Paper and Discussion September 26 (Dr. Steve Stice) September 28 Cloning: A Dolly Story September 30 Cloning in Domestic Livestock: Is it a viable option October 3 Mid-Term Paper Prep Day (No Class Today) October 5 Midterm Paper Due October 7 Cloning: Pets and Endangered Species October 10 DNA and RNA Analysis using PCR and RT-PCR (Group A) October 12 DNA and RNA Analysis using PCR and RT-PCR (Group B) October 14 Student Selected Paper and Discussion October 17 Human Therapeutic Cloning: The Nexus of Stem Cells and Cloning October 19 Ethics of Cloning October 21 Student Selected Paper and Discussion October 24 Disease Resistant Transgenic Animals October 26 Large Animal Models for Disease and Injury October 28 Fall Break No Class October 31 Large Animal Models for Disease and Injury November 2 Xenotransplantation November 4 Student Selected Paper and Discussion November 7 Pharm Animals November 9 Test 2 November 11 Student Selected Paper and Discussion November 14 Flow Cytometry A (Ms. Julie Nelson) November 16 Flow Cytometry B (Ms. Julie Nelson) November 18 Student Selected Paper and Discussion November 21 Thanksgiving Break No Class November 23 Thanksgiving Break No Class November 25 Thanksgiving Break No Class November 28 Student Selected Paper and Discussion November 30 Student Selected Paper and Discussion

6 December 2 December 5 December 6 Student Selected Paper and Discussion Student Selected Paper and Discussion Student Selected Paper and Discussion