Product Datasheet. OCT4 Antibody NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet OCT4 Antibody NB Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 9 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 10/10/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB OCT4 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight Page 1 of 6 v.20.1 Updated 10/10/ ml 1.0 mg/ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Immunogen affinity purified Tris-Glycine and 0.15M NaCl 40 kda Product Description Host Rabbit Gene ID 5460 Gene Symbol Species Reactivity Notes Marker Immunogen Product Application Details Applications POU5F1 Human, Mouse, Rat, Primate Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues. Embryonic Stem Cell Marker A synthetic peptide made to an internal portion of the human OCT4 protein (between residues ) [UniProt# Q01860] Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence Recommended Dilutions Western Blot 1:500, Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200 Application Notes This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kda. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. WB from verified customer review.

3 Images Western Blot: OCT4 Antibody [NB ] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines. Page 2 of 6 v.20.1 Updated 10/10/2018 Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB ] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody. Flow Cytometry: OCT4 Antibody [NB ] - An intracellular stain was performed on Jurkat with OCT4 Antibody NB and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/ml for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA , Thermo Fisher). Western Blot: OCT4 Antibody [NB ] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.

4 Publications Page 3 of 6 v.20.1 Updated 10/10/2018 Han YM, Bedarida T, Ding Y et al. B-Hydroxybutyrate Prevents Vascular Senescence through hnrnp A1-Mediated Upregulation of Oct4. Mol. Cell Sep :00AM [PMID: ] (ICC/IF, Mouse) Lupicka M, Socha B, Szczepanska A, Korzekwa A. Expression of pluripotency markers in the bovine uterus with adenomyosis. Reprod Biol Endocrinol 2015 [PMID: ] (WB) Lin HY, Lee DC, Wang HD et al. Activation of FGF1B Promoter and FGF1 Are Involved in Cardiogenesis Through the Signaling of PKC, but Not MAPK. Stem Cells Dev 2015 Dec 15 [PMID: ] Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A Jul 30 [PMID: ] (WB, Human) Darekar SD, Mushtaq M, Gurrapu S et al. Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts. Oncotarget May 12 [PMID: ] (WB, Rat) Details: OCT4 antibody was used for WB analysis of lysates from primary rat skin fibroblasts /RSFs and immortalized cells namely clone 17, clone 13 and clone 6. Oct4 was found to be induced in all clones of immortalized RSFs compared with the parental cells (Figure 2d). Liu JW, Hsu YC, Kao CY et al. LIF-induced Stat3 signaling suppresses FGF1-induced Erk1/2 activation to inhibit the downstream differentiation in mouse embryonic stem cells Stem Cells Dev 2012 Dec 4 [PMID: ] Cho YM, Kim YS, Kang MJ et al. Long-term recovery of irradiated prostate cancer increases cancer stem cells Prostate 2012 Dec 1 [PMID: ] (WB, Human) Ruggeri RR, Watanabe Y, Meirelles F et al. The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions J Assist Reprod Genet 2012 Sep 29 [PMID: ] (ICC/IF, Bovine) Yenamandra SP, Darekar SD, Kashuba V et al. Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts. Cell Death Dis 2012 Jan [PMID: ] (WB, Rat)

5 Procedures Protocol specific for OCT4 Antibody (NB ) Western Blot Protocol 1. Perform SDS-PAGE (4-12%) on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus. 3. Rinse membrane with dh2o and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil. 4. Rinse the blot in TBS for approximately 5 minutes. Page 4 of 6 v.20.1 Updated 10/10/ Block the membrane using 5% non-fat dry milk + 1% BSA in TBS for 1 hour at room temperature (RT). 6. Rinse the membrane in dh2o and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each. 7. Dilute the rabbit anti-oct4 primary antibody (NB ) in blocking buffer and incubate 1 hour at RT. 8. Rinse the membrane in dh2o and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each. 9. Apply the diluted rabbit-igg HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. 10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background). 11. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL). Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of %, provided it does not interfere with antibody-antigen binding.

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB800-PC1 NB PEP HAF008 NB7156 NBP HeLa Whole Cell Lysate OCT4 Blocking Peptide Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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