W HEN NOT HING ELSE MAT T ERS MORE T HAN ACCU RAT E RESULT S

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1 Alliace HPLC Bioseparatios System T he Alliace HPLC Bioseparatio System features: Biocompatible flow path (Titaium/PEEK) Auto Bled Techology, which bleds up to four eluets to perform isocratic, step, cotiuous gradiet, or flow program applicatios Automated SystemPREP routie for rapid uatteded system equilibratio W HEN NOT HING ELSE MAT T ERS MORE T HAN ACCU RAT E RESULT S I the ever chagig world of Biopharmaceuticals, reliable ad robust separatio techologies are eeded to keep pace with this fast growig area of discovery. System accuracy ad reproducibility mea everythig to scietists who stake their reputatios o the results they geerate or methods they develop. The Alliace HPLC Bioseparatios System, a high-precisio, high-resolutio HPLC system for the fractioatio ad aalysis of complex biomolecule mixtures, is ideally suited to help accelerate the progress of your biotechology aalyses. The core of the Alliace HPLC Bio System is the Waters e2796 Bioseparatios Module. Desiged for robust ad reliable operatio uder the harsh salt ad ph coditios typical of bioseparatios, it features a iert flow path, makig it ideal for protei purificatio, peptide mappig, ad ucleic acid aalysis. Precise temperature cotrol for ehaced sample stability Compatibility with highpressure ad low-pressure colum chemistries Automated colum switchig, flow diversio, ad fractio maagemet to support comprehesive multi-dimesioal separatios methods Ehaced system moitorig fuctios (ph, coductivity, compositio, valve, ad fractio trackig) for methods developmet ad troubleshootig Prove day-to-day reproducibility The Alliace HPLC Bioseparatios System may be cofigured to provide fully automated purificatio platforms for proteis, peptides, ad other biomolecules.

2 Available olie ph ad coductivity moitors eable you to optimize complex separatios High sesitivity UV/Visible usig real time buffer compositio or PDA detectio allows for iformatio. the simultaeous quatitatio of both major ad mior compoets. Switchig valves expad the system s capability Sample temperature cotrol to iclude ehaces biomolecule stability higher peak prior to aalysis. capacity, multidimesioal separatios. Titaium flow path is ideal for biomolecule separatios usig all moder bioseparatio chemistries. BioSuite HPLC Colums are Quaterary eluet bledig Samples ca be processed from eables the automatic stadard 96-, shallow- ad deep- formulatio of buffers from well plates, 384-well microtiter simple stock solutios plates ad custom plates, usig Auto Bled methods. 2-mL vials, or 0.5- ad 1.5-mL microcetrifuge tubes. high performace chemistries developed specifically for the isolatio, aalysis, ad characterizatio of proteis, peptides, ad other biomolecules.

3 INT EGRAT ED FLUIDIC S FOR CONSIST ENT P E RFORMANC E AND ENHANC ED RE P RODUCIBILIT Y The Waters e2796 Bioseparatios Module is the core of the system. Its itegrated solvet maagemet ad sample maagemet provides cosistet performace ad ehaced reproducibility for high quality, high-throughput aalysis for HPLC, LC/MS, or flow ijectio-mass spectrometry applicatios. Solvet maagemet Sample maagemet The simplified desig of the e2796 Solvet Maager eables the The desig of the e2796 Sample Maager esures precise samplig bledig ad deliverig of solvets i a pulse-free, low-dispersio deliverig predictable peak areas, ad cosistet results. flow path. Complimetig the simplified desig of the solvet flow path, a user I additio, Sychroized Compositio Cotrol (SCC ) of the gradiet proportioig valve (GPV) esures that the e2796 Separatios Module delivers excellet gradiet compositio accuracy ad precisio. The overall desig results i reduced complexity ad ehaced overall ruggedess across a flow rage that covers two orders of magitude. programmable eedle wash, ad selectable purge-wash-purge cycle esures that ay part of the system exposed to sample is fully washed, virtually elimiatig the risk of sample carryover. The iheret versatility of the three-axis (X, Y, Z) eedle assembly of the e2796 Sample Maager allows for greater applicatios flexibility. The sample maager has a wide rage of sample loops, holdig loops, ad syrige sizes available ad ca process samples Dual pressure trasducers eable true pulse-free solvet flow Idepedetly drive pistos achieve smooth solvet flow without pulse dampeers reducig gradiet volume from a variety of plates, vials, ad/or tubes. This variety allows High-efficiecy vacuum degassig techology you to customize the cofiguratio of the sample maager accordig to the eeds of your applicatios. Auto Bled Techology The e2796 Separatios Module features Auto Bled Techology, which eables you to geerate gradiets automatically without Tool-free routie maiteace o plugers/seals/seal-wash seals Simplified flow path with oly two ilet check valves Precise solvet bledig delivers highly reproducible gradiets havig to maually alter the buffer cocetratio. Auto Bled programmig techology improves uptime sice o premixig of mobile phases is required. Iitial Coditios Automated system start-up 90% The Alliace HPLC Bio System also features SystemPREP a Water useful whe you are chagig solvets or after exteded periods of system iactivity ad is a simple-to-use alterative to ruig the idividual fuctios of Wet Prime, Purge Ijector, Equilibrate, ad Coditio Colum. B 100 mm Ammoium Formate 90% 5 mm buffer complete system readiess for ruig samples. SystemPREP is Acetoitrile 5 mm buffer automated steps, puttig the separatios module i a coditio of A programmable, automated system preparatio routie for fast, oe-touch, walk-up operatio. This fuctio performs a series of fial Coditios 0% 0% C 100 mm Ammoium Bicarboate D Iitial fial

4 DO MORE W IT H YOU R RESULT S Waters software solutios eable you to capitalize o the results geerated by your system. The Waters Laboratory Iformatics Suite icludes prove iformatio maagemet solutios that itegrate all your lab resources i a cosistet, scalable, ad seamless applicatio eviromet. Empower Software provides cotrol MassLyx Software, for advaced NuGeesis SDMS is a customizable scie- of the system, icludig data acquisitio, mass spectrometry applicatios, eables tific iformatio maagemet platform that maagemet, processig, ad reportig, all researchers to acquire, aalyze, maage, automatically captures ad stores data i i the market-leadig chromatography data ad distribute iformatio. MassLyx a cetralized data warehouse. SDMS makes software (CDS) platform. Empower Software Software offers flexible cotrol over complex iformatio easier to access, facilitates is scalable from a sigle workstatio to a istrumet cofiguratios, ad Applicatio regulatory compliace, ad aids i the eterprise-wide CDS solutio, ad offers Maagers that are tailored for specific MS admiistratio of itellectual property. SDMS advaced features for method developmet, aalyses. coverts scietific results ito accessible ad reportig, ad custom calculatios, as well valuable corporate kowledge. as Eterprise Optios for advaced data maagemet ad automated method validatio maagemet. L EADING BIOSE PA RAT IONS CONSUMABL ES Whe your work ivolves the aalysis of proteis, peptides, ad amio acids, Waters has a variety of chemistry cosumable solutios for biomolecule separatios i areas ragig from proteomics to the validatio of well-characterized biopharmaceuticals. Waters BioSuite HPLC Colums. BioSuite HPLC Colums Peptide separatio techology colums Desiged specifically for bioseparatios, Waters lie of BioSuite Peptide Separatio Techology is a uique colum chemistry that HPLC Colums deliver the resolutio ad recovery of proteis ad meets the demadig requiremets of peptide separatios used i peptides scietists eed. Available i io exchage, size exclusio, a rage of applicatios. Available i 130 Å ad 300 Å pore sizes, hydrophobic iteractio, ad reversed-phase modes, BioSuite HPLC Peptide Separatio Techology colums are available i umerous Colums are available i aalytical as well as lab scale prepara- particle sizes ad colum dimesios for cosistet separatios, tive dimesios. from high-sesitivity proteomics, to peptide mappig, to high mass load purificatio. Peptide Separatio Techology colums are specifically QC tested with a protei digest to esure exceptioal applicatio performace. BE ASSURED. CHOOSE WAT ERS GLOBAL SERVIC ES. With 94 offices i more tha 50 coutries, oly the Waters Global Services team has the most i-depth ad up-to-date kowledge of the advaced sciece ad techologies that provide the foudatio for Alliace HPLC Systems. Maximize system uptime, icrease laboratory productivity, ad meet striget compliace requiremets with the expertise ad resposiveess you eed.

5 Sales Offices: Austria Australia Belgium ad Luxembourg Brazil Caada Chia Czech Republic Demark Filad Frace Germay Hog Kog Hugary Idia Irelad Italy Japa Korea Mexico The Netherlads Norway Polad Puerto Rico Russia/CIS / Sigapore Spai Swede Switzerlad Taiwa UK US Waters Corporatio 34 Maple Street Milford, MA U.S.A. T: F: Waters, Alliace, ad NuGeesis are registered trademarks of Waters Corporatio. The Sciece of What s Possible, Auto Bled, BioSuite, Sychroized Compositio Cotrol, SCC, Empower, ad MassLyx are trademarks of Waters Corporatio. All other trademarks are the property of their respective owers Waters Corporatio. Produced i the U.S.A. November EN LB-PDF