ExProfile TM Human Breast Cancer & Estrogen Receptor Signaling Related Gene qpcr Array

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1 Product Data Sheet ExProfile TM Human Breast Cancer & Estrogen Receptor Signaling Related Gene qpcr Array For focused group profiling of human breast cancer and estrogen receptor signaling genes expression Cat. No. QG005-A (1 x 96-well plate, Format A) Cat. No. QG005-B (1 x 96-well plate, Format B) Cat. No. QG005-C (1 x 96-well plate, Format C) Cat. No. QG005-D (1 x 96-well plate, Format D) Cat. No. QG005-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human breast cancer and estrogen receptor signaling related gene qpcr array profiles the expression of 84 human genes related to breast cancer regulation and estrogen receptor-dependent signal transduction. These genes are carefully chosen for their close pathway correlation based on a thorough literature search of peer-reviewed publications, mainly including genes involved in breast cancer, estrogen receptor-dependent signal pathway, breast cancer prognosis and cancer cells' response to chemotherapy. This array allows researchers to study the pathway-related genes to gain understanding of their roles in breast cancer regulation and estrogen receptor-dependent signal pathway. QG005 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) B (96-well) Applied Biosystems C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control Product Data Sheet 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG005 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Product Data Sheet Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ FAS QG A02 HQP NM_ CD44 QG A03 HQP NM_ HPRT1 QG A04 HQP NM_ AR QG A05 HQP NM_ VEGFA QG A06 HQP NM_ TP53 QG A07 HQP NM_ TOP2A QG A08 HQP NM_ TNFAIP2 QG A09 HQP NM_ TIE1 QG A10 HQP NM_ THBS2 QG A11 HQP NM_ THBS1 QG A12 HQP NM_ TGFA QG B01 HQP NM_ TFF1 QG B02 HQP NM_ STC2 QG B03 HQP NM_ SPRR1B QG B04 HQP NM_ SERPINE1 QG B05 HQP NM_ SERPINB5 QG B06 HQP NM_ SERPINA3 QG B07 HQP NM_ SCGB2A1 QG B08 HQP NM_ RPL27 QG B09 HQP NM_ RAC2 QG B10 HQP NM_ PTGS2 QG B11 HQP NM_ PTEN QG B12 HQP NM_ PLAU QG C01 HQP NM_ PGR QG C02 HQP NM_ PAPPA QG C03 HQP NM_ NME1 QG C04 HQP NM_ NGFR QG C05 HQP NM_ NGFB QG C06 HQP NM_ NFYB QG C07 HQP NM_ MUC1 QG C08 HQP NM_ MT3 QG C09 HQP NM_ MKI67 QG C10 HQP NM_ MAP2K7 QG C11 HQP NM_ KRT19 QG C12 HQP NM_ KRT18 QG D01 HQP NM_ KLK5 3

4 Product Data Sheet QG D02 HQP NM_ KLF5 QG D03 HQP NM_ KIT QG D04 HQP NM_ JUN QG D05 HQP NM_ ITGB4 QG D06 HQP NM_ ITGA6 QG D07 HQP NM_ IL6ST QG D08 HQP NM_ IL6R QG D09 HQP NM_ IL6 QG D10 HQP NM_ IL2RA QG D11 HQP NM_ IGFBP2 QG D12 HQP NM_ ID2 QG E01 HQP NM_ HSPB1 QG E02 HQP NM_ HMGB1 QG E03 HQP NM_ GATA3 QG E04 HQP NM_ GABRP QG E05 HQP NM_ FOSL1 QG E06 HQP NM_ FGF1 QG E07 HQP NM_ FASLG QG E08 HQP NM_ FAS QG E09 HQP NM_ ESR2 QG E10 HQP NM_ ESR1 QG E11 HQP NM_ ERBB2 QG E12 HQP NM_ EGFR QG F01 HQP NM_ DLC1 QG F02 HQP NM_ CYP19A1 QG F03 HQP NM_ CTSD QG F04 HQP NM_ CTSB QG F05 HQP NM_ CTNNB1 QG F06 HQP NM_ COL6A1 QG F07 HQP NM_ CLU QG F08 HQP NM_ CDKN2A QG F09 HQP NM_ CDKN1B QG F10 HQP NM_ CDKN1A QG F11 HQP NM_ CD44 QG F12 HQP NM_ CCNE1 QG G01 HQP NM_ CCND1 QG G02 HQP NM_ CCNA2 QG G03 HQP NM_ CCNA1 QG G04 HQP NM_ C3 QG G05 HQP NM_ BCL2L2 QG G06 HQP NM_ BCL2 4

5 Product Data Sheet QG G07 HQP NM_ BAG1 QG G08 HQP NM_ BAD QG G09 HQP NM_ AR QG G10 HQP NM_ CDH1 QG G11 HQP NM_ CLDN7 QG G12 HQP NM_ FLRT1 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Product Data Sheet Following terms and conditions apply to use of ExProfile TM Human Breast Cancer & Estrogen Receptor Signaling Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG