B.Sc. DEGREE (CBSC) EXAMINATION. (Examination at the end of Sixth semester) Biochemistry. Microbiology and Molecular Biology- VII A

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1 B.Sc. DEGREE (CBSC) EXAMINATION (Examination at the end of Sixth semester) Biochemistry Microbiology and Molecular Biology- VII A Time : Three hours Maximum : 75 marks 1. Bacterial growth curve. 2. Lytic cycle of λ phage. 3. Immobilized enzymes. 4. Enzymes of DNA replication. 5. Characterstics of genetic code. 6. Inhibitiors of protein synthesis. 7. PCR 8. Genomic libraries SECTION A (5 x 5 = 25 MARK) SECTION B (5 x 10 = 50 MARKS) Answer ALL questions. UNIT 1 9. a) Discuss about the isolation and cultivation of bacteria? b) Write about the life cycle and structure of HIV? UNIT II 10. a) Describe about the mechanism of transcription in prokaryotes? b) Describe the mechanism of DNA replication? UNIT III 11. a) Write about the mechanism of protein synthesis? b) Explain about the regulation of prokaryotic gene expression by lac operon?

2 UNIT IV 12. a) Explain the DNA sequencing methods? b) Define blotting techniques and give note on different types of blotting techniques? UNIT V 13. a) Give basic outlines of fermentation techniques? b) Discuss about the production of transgenic plant in brief & give their applications?

3 B.Sc. DEGREE (CBCS) EXAMINATION (Examination at the end of sixth semester) Part III - Biochemistry Cluster Electives VIII-Medical diagnostics HAEMATOLOGY VIII-1 Time: Three hours Maximum: 75 marks 1. Anticoagulants. 2. ESR 3. Haemoglobin synthesis. 4. Fibrinolytic mechanism. 5. Sickle cell Anemia. 6. Life cycle of Wuchereria bancrofti. 7. Blood cell counters. 8. Human blood group system. SECTION A- (5x5 =25 marks ) SECTION B (5x10=50 marks) UNIT I 9. a) Discuss about the basic requirements for the collection and storage of blood? b) Describe about the effect of anti coagulants on blood cells morphology? UNIT II 10. a) Discuss about the Erythropoieses? 11. b) Clinical significance of blood cell counts? UNIT III 12. a) Explain the structure and life cycle of Plasmodium? b) Discuss about the haematological diseases? UNIT IV 13. a) Discuss about automated coagulated systems? b) Write about the mechanism of blood coagulation?

4 UNIT V 14. a) Give brief account on inheritance of blood group systems? b) Give the outlines of blood transfusion?

5 B.Sc. DEGREE (CBCS) EXAMINATION (Examination at the end of the sixth Semester) Part II Biochemistry Cluster Electives VIII-Medical diagnostics CLINICAL MICROBIOLOGY VIII--2 Time : Three hours Maximum : 75 marks. SECTION A (5 5 = 25 marks) 1. Factors effecting the bacterial growth. 2. Sterilization by heat. 3. Acid fast Staining. 4. Types of culture media. 5. Mycobacterium tuberculosis. 6. Superficial mycoses. 7. Measles. 8. Agglutination tests. SECTION B (5 10 = 50 marks) Answer ALL questions. UNIT I 9. a) Discuss about the normal bacterial flora of the body. b) Discuss about the classification of the bacteria. UNIT II 10. a) Write a note on sterilization and disinfection techniques. b) Explain about safe disposal strategies to be followed in microbiology lab.

6 UNIT III 11. a) Discuss about the quality control methods of various culture media. b) Give notes on staining reaction, cultural characteristics and biochemical properties of gram -ve bacteria. UNIT IV 12. a) Discuss about the morphological classification of clinically important fungi. b) Discuss about A) Polio virus. B) Hepatitis virus. UNIT V 13. a) Give notes on Autoimmune Diseases. b) Discuss about the principles of Serodiagnostic test

7 B.Sc. DEGREE (CBCS) EXAMINIATION (Examination at the end of the sixth Semester) PART II-BIOCHEMISTRY Cluster Electives VIII-Medical diagnostics BIOCHEMICAL CORRELATIONS IN DISEASES VIII-3 Time : Three hours Maximum : 75Marks SECTION A (5 Χ 5=25marks) 1. Phenylketonuria. 2. Lipid storage diseases. 3. Protein-calorie malnutrition. 4. Wilson s disease. 5. Systemic lupus erythematosus 6. Self & non-self discrimination by immune system. 7. Antigenic shift & Antigenic drift. 8. Vaccines. SECTION B (5 5 = 50 marks) Answer ALL questions. UNIT I 9. a) Discuss about the glycogen storage diseases in detail. b) Write notes on diseases caused due to misfolded proteins. 10. a) Discuss about any three vitamin deficiency diseases. b) Discuss about the life style disease and the consequences related to it. 11. a) Define Auto immune diseases, write about organ specific Auto immune diseases. b) Give an account on hormone imbalance diseases taking example as thyroid hormones. 12. a) Discuss about any present emerging viral infections. b) Give overall view on bacterial pathogenesis. 13. a) Discuss about Polio virus infection. b) Discuss about any parasitic pathogen infections