ExProfile TM Human Angiogenesis Related Gene qpcr Array

Transcription

1 Product Data Sheet ExProfile TM Human Angiogenesis Related Gene qpcr Array For focused group profiling of human angiogenesis genes expression Cat. No. QG002-A (1 x 96-well plate, Format A) Cat. No. QG002-B (1 x 96-well plate, Format B) Cat. No. QG002-C (1 x 96-well plate, Format C) Cat. No. QG002-D (1 x 96-well plate, Format D) Cat. No. QG002-E (1 x 96-well plate, Format E) Plates available individually or as a set of 6. Each set contains 84 unique gene primer pairs deposited in one 96-well plate. Introduction The ExProfile human angiogenesis related gene qpcr array profiles the expression of 84 human genes related to angiogenesis. These genes are carefully chosen for their close correlation based on a thorough literature search of peer-reviewed publications, mainly including genes that encode various angiogenic factors and other molecules involved in angiogenesis. This array allows researchers to study the related genes to gain understanding of their roles in the functioning and characterization of angiogenesis. QG002 plate 01: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) B (96-well) Applied Biosystems Applied Biosystems 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7 (Fast block) C (96-well) Bio-Rad Laboratories icycler iq,myiq,iq 5 D (96-well) Bio-Rad Laboratories CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 E (96-well) Roche Applied Science LightCycler 480 (96-well block) 1

2 Quality control Product Data Sheet 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG002 plate 01 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. 2

3 Gene primer list Product Data Sheet Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ HGF QG A02 HQP NM_ COL18A1 QG A03 HQP NM_ HGF QG A04 HQP NM_ NRP2 QG A05 HQP NM_ HPRT1 QG A06 HQP NM_ VEGFC QG A07 HQP NM_ VEGFA QG A08 HQP NM_ TNFAIP2 QG A09 HQP NM_ TNF QG A10 HQP NM_ TIMP3 QG A11 HQP NM_ TIMP2 QG A12 HQP NM_ TIMP1 QG B01 HQP NM_ THBS2 QG B02 HQP NM_ THBS1 QG B03 HQP NM_ TGFBR1 QG B04 HQP NM_ TGFB2 QG B05 HQP NM_ TGFB1 QG B06 HQP NM_ TGFA QG B07 HQP NM_ TEK QG B08 HQP NM_ STAB1 QG B09 HQP NM_ SPHK1 QG B10 HQP NM_ SERPINF1 QG B11 HQP NM_ PTGS1 QG B12 HQP NM_ PROK2 QG C01 HQP NM_ PLXDC1 QG C02 HQP NM_ PLG QG C03 HQP NM_ PLAU QG C04 HQP NM_ PECAM1 QG C05 HQP NM_ PDGFA QG C06 HQP NM_ NRP2 QG C07 HQP NM_ NRP1 QG C08 HQP NM_ MMP9 QG C09 HQP NM_ LEP QG C10 HQP NM_ KDR QG C11 HQP NM_ JAG1 QG C12 HQP NM_ ITGB3 QG D01 HQP NM_ ITGAV 3

4 Product Data Sheet QG D02 HQP NM_ IL8 QG D03 HQP NM_ IL6 QG D04 HQP NM_ IL1B QG D05 HQP NM_ IGF1 QG D06 HQP NM_ IFNG QG D07 HQP NM_ IFNB1 QG D08 HQP NM_ IFNA1 QG D09 HQP NM_ ID3 QG D10 HQP NM_ ID1 QG D11 HQP NM_ HPSE QG D12 HQP NM_ HIF1A QG E01 HQP NM_ HGF QG E02 HQP NM_ HAND2 QG E03 HQP NM_ FLT1 QG E04 HQP NM_ FIGF QG E05 HQP NM_ FGFR3 QG E06 HQP NM_ FGF2 QG E07 HQP NM_ FGF1 QG E08 HQP NM_ EREG QG E09 HQP NM_ EPHB4 QG E10 HQP NM_ ENG QG E11 HQP NM_ EGF QG E12 HQP NM_ EFNB2 QG F01 HQP NM_ EFNA3 QG F02 HQP NM_ EFNA1 QG F03 HQP NM_ EDG1 QG F04 HQP NM_ ECGF1 QG F05 HQP NM_ CXCL9 QG F06 HQP NM_ CXCL6 QG F07 HQP NM_ CXCL5 QG F08 HQP NM_ CXCL3 QG F09 HQP NM_ CXCL10 QG F10 HQP NM_ CXCL1 QG F11 HQP NM_ CDH5 QG F12 HQP NM_ CCL2 QG G01 HQP NM_ CCL11 QG G02 HQP NM_ BAI1 QG G03 HQP NM_ ANPEP QG G04 HQP NM_ ANGPTL4 QG G05 HQP NM_ ANGPTL3 QG G06 HQP NM_ ANGPT2 4

5 Product Data Sheet QG G07 HQP NM_ AKT1 QG G08 HQP NM_ ANGPT1 QG G09 HQP NM_ COL18A1 QG G10 HQP NM_ COL4A3 QG G11 HQP NM_ PF4 QG G12 HQP NM_ LECT1 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 5

6 Limited Use License Product Data Sheet Following terms and conditions apply to use of ExProfile TM Human Angiogenesis Related Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX,ViiA TM,StepOnePlus TM (Life Technologies). QG