Based on real needs our breakthroughs improve our customers production processes and products. I N N O V A T I O N R E I M A G I N E D

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1 Based on real needs our breakthroughs improve our customers production processes and products. I N N O V A T I O N R E I M A G I N E D

2 F O U R T A R G E T M A R K E T S Bioprocesses, biological water treatment, agriculture, pharma Microelectronics, automotive, computer, aerospace, battery Chemical water treatment, antibacterial applications, purification, filtration Cosmetics, cosmeto-textiles, fragrances, pharma, Fire retardant

3 OUR PRODUCTS Silica particles Particle size : 500 nm, 10 µm or 30 µm Hollow amorphous silica (non toxic) Magnetic option Porous material Mesoporous (Pores : nm) High surface area (>100m2/g or 2x10 7 m 2 /m 3 ) Ideal for enzyme immobilization & fermentation Functionalized material Can be functionalized with polar and/or nonpolar groups Dispersible in water or other organic solvents Temperature process up to 300 C

4 The impact of MATSPHERES technology on: 1. Enzyme immobilisation 2. Recycling of enzymes 3. Fermentation

5 ENZYME STABILITY Increase stability Protect from harsh environment Extended lifetime (2h vs 30 min) Increase productivity Promote higher activity 300% peroxide production Increase Activity 120% enzymatic activity

6 ENZYME RECYCLING Recycling Filtration recycling More efficient than polymer bound Magnetic recycling A A: Magnetic MATSPHERES without magnet B: Magnetic MATSPHERES with magnet B

7 Relative activity (%) Relative Activity (%) ACTIVITY LEVELS OF RECYCLED ENZYMES MATSPHERES Magnetic MATSPHERES Recycling cycles Recycling cycles Lipase recyling using filtration Laccase recyling using magnetic MATSPHERES

8 Enzymatic Activity ENZYME LEACHING Low leaching Enzyme covalently bound on MATSPHERES Low leaching over time Time Laccase Leaching 6 h 1.5 % 24 h 3.5 % Compatible with different chemistries Stable at room temperature - After 50 days of storage : Before washing with strong detergent : After washing Stable with strong chemical - After 60 days of storage A B C D E F G H I Lipase immobilized by different chemistries

9 FERMENTATION Benefits Increase productivity Reduce incubation time Increase ease of operation

10 FERMENTATION PRODUCTIVITY Increase productivity 40% more hyaluronic acid 30 % more biogas 60% more ammonium consumed Reduce incubation time 1 day vs 7 days (plastic media)

11 Increase ease of operation FERMENTATION PROCESS Reduce mold pellet formation Suspend biofilms Mold : Rhizopus oryzae Antifoaming effect Without MATSPHERES Low concentration MATSPHERES High concentration MATSPHERES Grafting option for plastic media Without MATSPHERES With MATSPHERES

12 The value of MATSPHERES technology: 1. Increase productivity of enzymes 2. Recycling of enzymes 3. Increase productivity of microorganisms 4. Ease of operation

13 PURIFICATION AND CONCENTRATION Concentration of biomolecules Biomolecules can be adsorbed on MATSPHERES High surface area (2x10 7 m 2 /m 3 ) High loading rate Different function available Amine Vinyl Hydroxyl Graphene

14 PHYSICOCHEMICAL TREATMENT TEST RESULTS Chemical and biochemical industry related compounds Concentration used Adsorption Class Adsorbent Example MATSPHERES (g/l) Pollutant (ppm) capacity (mg/g) Odors from aldehydes MATSPHERES 200-A Furfural Odors from aromatics MATSPHERES 200-AV Vaniline Polyphenols MATSPHERES 200-A Catechol Terpenes MATSPHERES 200-SG Farnesol Fatty acids MATSPHERES 200-A Butyric acid Sugars MATSPHERES 200-A Glucose Proteins MATSPHERES 200-AV Bovine serum albumin Hormone MATSPHERES 200-GO Estrone DNA MATSPHERES 200-G DNA Ammonium MATSPHERES 200-AV Ammonium Phosphate MATSPHERES 200-A Phosphate Sulphate MATSPHERES 200-AA Sulfate Heavy metals MATSPHERES 200-AV (Solution of Al, Ca, Cu, Fe, K, Li, Mg, Mn, Na, Ni, Pb, S, Zn) Polycyclic Aromatic (Mix of Fluorene, Naphthalene and MATSPHERES 200-AV Hydrocarbons (PAHs)* Phenanthrene) Oil MATSPHERES 200-AV Vegetable oil *Performed in hexanes (solvent for PAHs) Note : MATSPHERES concentrations are on a dry basis

15 P A R T N E R I N G F O R I N N O V A T I O N Mathilde Gosselin, CEO Office: +1 (450) Cell: +1 (450) mgosselin@materium.ca , boul. industriel, Granby, Québec, Canada