Product Datasheet. IL-1 beta/il-1f2 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 4 Publications: 15

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1 Product Datasheet IL-1 beta/il-1f2 Antibody NB Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 4 Publications: 15 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 9/6/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB IL-1 beta/il-1f2 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity 0.1 mg Please see the vial label for concentration. If unlisted please contact technical services. Store at -20C. Avoid freeze-thaw cycles. Polyclonal No Preservative IgG IgG purified Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, ph 7.2 Product Description Host Rabbit Gene ID 3553 Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications IL1B Human, Mouse, Rat, Porcine, Bacteria, Canine, Primate Page 1 of 5 v.20.1 Updated 9/6/2018 Mouse reactivity reported in scientific literature (PMID: ) Rat reactivity reported in scientific literature (PMID: ). Porcine reactivity reported in scientific literature (PMID: ). Bacteria reactivity reported in scientific literature (PMID: ). Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kda precursor molecule but strongly detects all of the 17 kda mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kda precursor molecule with greater affinity than the native form, but will often detect past the 35 kda mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays. This antibody was prepared by repeated immunizations with recombinant human IL-1 beta/il-1f2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kda with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1). Western Blot, ELISA, Electron Microscopy, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation Recommended Dilutions Western Blot 1:1000, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Electron Microscopy 1:10-1:500

3 Application Notes Page 2 of 5 v.20.1 Updated 9/6/2018 Anti-Human IL-1B has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1B. For immunoblots, typically, IL-1B is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/ml E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1B. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1B activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1B. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically. Images Western Blot: IL-1 beta/il-1f2 Antibody [NB ] - Lane 1: Human IL-1Beta. Load: 5 ng per lane. Primary antibody: Human IL-1Beta antibody at 1:2,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 17 kda, 17 kda for Human IL-1Beta. Other band(s): Unspecific band at 35 kda. Immunocytochemistry/Immunofluorescence: IL-1 beta/il-1f2 Antibody [NB ] - Trabecular Meshwork (TM) region of pig eyes were stained with IL-1 beta antibody (red). Image from verified customer review.

4 Immunohistochemistry: IL-1 beta/il-1f2 Antibody [NB ] - Analysis of: Human IL1beta antibody Secondary antibody: Peroxidase goat antirabbit at 1:10,000 for 45 min at RT Localization: cytoplasm Staining: Close up of medullary lymph node: positive staining in the cytoplasm of circulating macrophages. Neg Ctr (far right) normal rabbit IgG with ph X Page 3 of 5 v.20.1 Updated 9/6/2018 Western Blot: IL-1 beta/il-1f2 Antibody [NB ] - Analysis of IL-1 beta in Rhesus fetal membrane using anti-il-1 beta antibody. Image from verified customer review. Western Blot: IL-1 beta/il-1f2 Antibody [NB ] - Antibody was used at a 1:200 dilution incubated 1h at room temperature to detect dog IL-1b by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1b respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye (TM) 800 conjugated Donkey anti-rabbit IgG for 1h at room temperature. Western Blot: IL-1 beta/il-1f2 Antibody [NB ] - pro-il-1beta in glioblastoma cells. Image from verified customer review.

5 Publications Cheng Ran, Liu Wen, Zhang Rui et al. Porphyromonas gingivalis-derived Lipopolysaccharide Combines Hypoxia to Induce Caspase-1 Activation in Periodontitis. Frontiers in Cellular and Infection Microbiology 2017 [PMID: ] (WB, Mouse) Bajpai G, Schneider C, Wong N et al. The human heart contains distinct macrophage subsets with divergent origins and functions Nat. Med. Jun :00AM [PMID: ] (IHC-P, Human) Stodle GS, Silva GB, Tangeras LH et al. Placental inflammation in pre-eclampsia by Nod-like receptor protein (NLRP)3 inflammasome activation in trophoblasts. Clin. Exp. Immunol. Apr :00AM [PMID: ] (IHC) Lin ZH, Wang SY, Chen LL et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia Front Cell Neurosci 2017 Dec 11 [PMID: ] (WB, Rat) Komiya H, Shimizu K, Noma N et al. Role of Neuron-Glial Interaction Mediated by IL-1b in Ectopic Tooth Pain J. Dent. Res Nov 01 [PMID: ] (WB) Ahlstrand T, Tuominen H, Beklen A et al. A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin- 1beta and interleukin-8. Virulence 2017 Feb 17 [PMID: ] Ahmad F, Chung YW, Tang Y et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep Jun :00AM [PMID: ] (WB, Mouse) Rossi AF, Cadamuro AC, Biselli-Perico JM et al. Interaction between inflammatory mediators and mirnas in Helicobacter pylori infection. Cell. Microbiol Mar 04 [PMID: ] (IHC-P, Human) Bimpis A, Papalois A, Voumvourakis K et al. Neuronal tumour necrosis factor-alpha and interleukin-1beta expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G Brain Res Apr 25 [PMID: ] (IHC-P, Porcine) Su B, Luo T, Zhu J et al. Interleukin-1B/IRAK-1 inflammatory signaling contributes to persistent gankyrin activation during hepatocarcinogenesis. Hepatology Oct 07 [PMID: ] (WB, IHC-P, Human, Rat) Arendt LM, McCready J, Keller PJ et al. Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis. Cancer research Oct 1 [PMID: ] (WB, IP, Mouse) Petrovski G, Ayna G, Majai G et al. Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1 beta release in human macrophages. Autophagy Mar [PMID: ] (WB, Human) More publications at Page 4 of 5 v.20.1 Updated 9/6/2018

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF008 NB7156 NBP NBP ug Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Recombinant Human IL-1 beta/il-1f2 Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: