DNA, RNA, and protein purification

Transcription

1 DNA, RNA, and protein purification Catalog supplement 2016 What s new?

2 Contents MACHEREY-NAGEL Bioanalysis catalog supplement 2016 This Bioanalysis catalog supplement 2016 is an addition to the main Bioanalysis catalog 2014 / The new MACHEREY-NAGEL products are sorted according to the product chapters in the main catalog. Product Clean-up NGS clean-up and size selection NucleoMag NGS Clean-up and Size Select Clean-up and tunable size-selection of NGS library preparation reactions Page 4 RNA RNA from cells and tissue NucleoSpin RNA Plus Ultra-fast isolation of high-quality RNA including lysate clearing and gdna removal in one step NucleoZOL High yield RNA isolation from all kind of sample material 6 MicroRNA Exosome Precipitation Solution (Serum / Plasma), (Urine) Simple and efficient exosome precipitation for quantitative mirna isolation 7 5 Contents Genomic DNA Genomic DNA from blood and biological fluids NucleoSpin Blood L Vacuum Convenient and time-saving DNA purification from large blood volumes under vacuum Genomic DNA from microorganisms NucleoSpin Microbial DNA Isolation of total DNA from hard-to-lyse microbial cultures with bead-supported homogenization Genomic DNA from FFPE samples NucleoSpin 96 DNA FFPE NucleoMag DNA FFPE Xylene-free high-throughput isolation and decrosslinking of DNA from FFPE samples Genomic DNA from forensic samples NucleoSpin Forensic Filters Incubation and lysate separation of solid sample carriers in one tube Viral RNA and DNA Viral RNA and DNA from serum and plasma NucleoSpin Virus Simultaneous isolation of viral RNA and DNA 13 Accessories Equipment NucleoMag SEP Mini Manual separation of magnetic beads in 1.5 ml or 2 ml reaction tubes NucleoMag SEP Maxi NucleoBond Smart Rack Starter Set Midi Magnetic separation in 50 ml tubes For processing of NucleoBond Columns by gravity flow For processing of NucleoSpin Midi / L Columns under vacuum Consumables NucleoSpin Bead Tubes Efficient cell disruption

3 MACHEREY-NAGEL Company updates MACHEREY-NAGEL Quality, innovation, and support Since 1911, MACHEREY-NAGEL stands for highly reliable analytical products made in Germany. Based on more than 100 years of experience, the company has focused on continuous improvement and innovations to offer up-to-date solutions of outstanding quality. MACHEREY-NAGEL Facilities and resources Throughout MACHEREY-NAGEL s centennial history, the companyʼs facilities have constantly been adapted and renewed. Just recently the production and the warehouse facilities have been modernized and enlarged. State-of-the-art high-quality equipment meets optimal production and delivery requirements. MN headquarters in Düren, Germany To share our long lasting knowledge and expertise with customers and distributors, a new training center has been established at the MACHEREY-NAGEL headquarters in Düren. A dedicated team of scientists and product specialists with extensive practical and theoretical expertise is committed to train our clients and provide maximal support. MACHEREY-NAGEL Packaging according to up-to-date standards To meet the demands of providing a reliable, high quality product, MACHEREY-MAGEL is always looking for enhancements and continuous optimizations of products and production processes. As of June 2015, all MACHEREY-NAGEL products will be labeled according to the Globally Harmonized System of Classification (GHS), the new world-wide-standardized system for classification and labeling of chemicals on product labels and Material Safety Data Sheets (MSDS). The most noticeable change is the new diamond shaped hazardous icon which replaced the previously labeled orange square symbols. MN Training-Center symbol Hazardous icons according to GHS Company updates In addition, all liquids included in the MACHEREY-NAGEL Bioanalysis product line will be provided in new Nalgene round bottles. These bottles provide better handling and a wider opening, allowing easier and more precise pipetting. The new bottles have shown improved stability and have helped streamline production processes. Nalgene round bottles 3

4 NGS clean-up and size selection NucleoMag NGS Clean-up and Size Select Clean-up and size selection of NGS (Next Generation Sequencing) library preparation reactions Convenient magnetic-bead technology easy to adjust for different applications or workflows optimal scalability for manual and automated processing Tunable size selection from 150 bp to 800 bp highest flexibility for custom-specific applications NucleoMag NGS Clean-up and Size Select Magnetic-bead technology Format Highly reactive superparamagnetic beads Processing Manual or automated Reaction mixtures from common NGS library kits Amount of sample material 7.5 pg 5 μg Fragment size Tunable: bp Input volume μl Typical recovery > 80 % Elution volume μl Preparation time min/96 preps Clean-up Product Pack of Specification REF NucleoMag NGS Clean-up and Size Select 5 ml 50 ml 500 ml NucleoMag NGS Bead Suspension Material to be supplied by the user Pack of Specification REF Square-well Block (separation plate) well blocks with 2.1 ml square-wells Elution Plate, U-bottom (elution plate) well microplates with 300 μl u-bottom wells, including Self-adhering Foil Product accessories Pack of Specification REF NucleoMag SEP 1 magnetic separator, for use with 96-well plates NucleoMag SEP Mini 1 magnetic separator, for use with 1.5 ml or ml reaction tubes (12 positions) NucleoMag SEP Maxi 1 magnetic separator, for use with 50 ml tubes (4 positions) For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 /

5 MicroRNA NucleoSpin RNA Plus NucleoSpin gdna Removal Column included New generation of RNA isolation from cells and tissue NucleoSpin gdna Removal Column included no time-consuming rdnase digestion Lysate clearing and gdna removal in one step quick and convenient handling New efficient lysis buffer no ß-mercapthoethanol or TCEP necessary NucleoSpin RNA Plus Two column silica-membrane system 1. column for DNA removal 2. column for RNA isolation Format Mini spin columns < 1 x 10 7 cultured cells, < 10 9 bacterial cells, < 10 8 yeast cells, < 30 mg human / animal tissue Fragment size > 200 nt Typical yield HeLa cells 5 x 10 6 : μg Mouse liver 20 mg: μg Mouse kidney 20 mg: μg Mouse spleen 5 mg: μg A 260 /A A 260 /A Typical RIN (RNA integrity number) > 9 Elution volume μl Preparation time 20 min/6 preps Binding capacity 200 μg Typical downstream applications Real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays RNA Product Preps Specification REF NucleoSpin RNA Plus NucleoSpin RNA Plus Columns with Collection Tubes, NucleoSpin gdna Removal Columns with Collection Tubes, Collection Tubes (2 ml and 1.5 ml), buffers For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 /

6 RNA from cells and tissue NucleoZOL High yield total RNA extraction from all kind of samples No phase separation No use of chloroform, no phase separation needed easy and safe procedure Efficient removal of DNA, proteins, and other contaminating compounds high RNA purity from any sample material Isolation of total RNA in one fraction or small and large RNA in two fractions flexible use Combination with NucleoSpin RNA Columns possible RNA /ml reagent (scalable) Fragment size Typical yield A 260 /A Typical RIN > 9 Elution volume Flexible Preparation time < 1 h NucleoZOL One-phase extraction < 1 x 10 7 cultured cells, bacteria, and yeast, < 100 mg human / animal / plant tissue, < 0.4 ml (viral) fluids Small RNA: nt, large RNA: > 200 nt Total RNA: Liver: Kidney, spleen: Muscle, brain, lung: Cultured cells: 6 8 μg/mg tissue 3 4 μg/mg tissue μg/mg tissue 4 10 μg/10 6 cells Large RNA: Liver: Kidney, spleen: Muscle, brain, lung: Cultured cells: 5 7 μg/mg tissue 3 4 μg/mg tissue μg/mg tissue 3 8 μg/10 6 cells Product Pack of Specification REF NucleoZOL 200 ml NucleoZOL Reagent Related products Preps Specification REF NucleoSpin RNA Clean-up NucleoSpin RNA Clean-up Columns with Collection Tubes, Collection Tubes (2 ml and 1.5 ml), buffers

7 MicroRNA Exosome Precipitation Solution (Serum / Plasma) Exosome Precipitation Solution (Urine) Exosome enrichment for most efficient total RNA isolation from serum and plasma samples, urine, other body fluids, and cell culture supernatants (serum-free) Simple and fast exosome precipitation avoidance of tedious ultra-centrifugation Flexibe sample amount procedure can be scaled up or down depending on sample volume Preparation time Typical downstream application Exosome Precipitation Solution Precipitation solution Flexible input: ml serum / plasma (Exosome Precipitation Solution (Serum / Plasma)) 1 10 ml urine (Exosome Precipitation Solution (Urine)) 45 min/6 preps Total RNA isolation (e.g., with NucleoSpin mirna Plasma, see ordering information) Product Pack of Specification REF Exosome Precipitation Solution (Serum / Plasma) Exosome Precipitation Solution (Urine) 2 ml 12 ml 60 ml 12 ml 50 ml 250 ml Exosome Precipitation Solution, RNase-free water Exosome Precipitation Solution, RNase-free water Related products Preps Specification REF NucleoSpin mirna Plasma NucleoSpin mirna Columns, Collection Tubes (2 ml), buffers, RNase-free rdnase RNA 7

8 Genomic DNA from blood and biological fluids NucleoSpin Blood L Vacuum DNA purification from up to 2 ml whole blood using vacuum filtration Efficient DNA purification consistent high yields and complete removal of PCR inhibitors Adapter set for the NucleoVac 96 Vacuum Manifold available parallel purification of 24 samples Genomic DNA NucleoSpin Blood L Vacuum Silica-membrane technology Format Midi spin columns 1 2 ml whole blood (human or animal, fresh or frozen, treated with citrate, heparin, or EDTA) Fragment size 200 bp approx. 50 kbp Typical yield μg (2 ml blood) A 260 /A Typical concentration ng/μl Elution volume 2 x 300 μl Preparation time 75 min/24 preps Binding capacity 250 μg Typical downstream PCR, Southern blotting, enzymatic reactions applications Product Preps Specification REF NucleoSpin Blood L Vacuum 24 NucleoSpin Blood L Columns with Collection Tubes, Collection Tubes (1.5 ml), buffers, Proteinase K Product accessories Pack of Specification REF NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum Starter Set Midi 1 for processing NucleoSpin Midi / L Columns under vacuum on NucleoVac 96 Vacuum Manifold or similar manifolds, contains 1 Column Holder Midi, 1 Wash Plate Midi, 1 Elution Tube Holder Midi, 24 Dummy Columns Midi For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 /

9 Genomic DNA from microorganisms NucleoSpin Microbial DNA Isolation of total DNA from hard-to-lyse microorganisms Suitability for a large variety of starting cultures high-quality DNA from gram-positive / gram-negative bacteria, yeast, or fungi with one procedure NucleoSpin Bead Tubes for efficient cell disruption compatibility with common disruption devices Liquid Proteinase K included no additional enzyme required Format NucleoSpin Microbial DNA Silica-membrane technology Mini spin columns < 40 mg wet weight cell pellet (gram-positive bacteria, gram-negative bacteria, yeast, fungi) Fragment size 200 bp approx. 50 kbp Typical yield Depends on sample type and disruption method Approx μg (30 mg wet weight cell pellet) A 260 /A Elution volume μl Preparation time 35 min/prep Binding capacity 60 μg Typical downstream PCR, real-time PCR, Southern blotting, enzymatic reactions applications Genomic DNA Product Preps Specification REF NucleoSpin Microbial DNA NucleoSpin Microbial DNA Columns, NucleoSpin Bead Tubes Type B, Collection Tubes (2 ml), buffers, Liquid Proteinase K Product accessories Pack of Specification REF NucleoSpin Bead Tubes Type A 50 2 ml tubes with ceramic beads (as included in the NucleoSpin Soil kit) NucleoSpin Bead Tubes Type B 50 2 ml tubes with glass beads (as included in the NucleoSpin Microbial DNA kit) NucleoSpin Bead Tubes Type C 50 2 ml tubes with corundum (recommended for yeast disruption) For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 /

10 Genomic DNA from FFPE samples NucleoSpin 96 DNA FFPE Isolation of genomic DNA from formalin-fixed, paraffin-embedded samples (fresh or archived) in 96-well plate format Blue colored Paraffin Dissolver (patent pending) for easy paraffin removal no use of harmful organic solvents Decrosslinking buffer to eliminate formalin crosslinking high-quality DNA for improved performance in downstream applications (e.g., PCR) Processing under vacuum or by centrifugation suitability for manual and automated processing Genomic DNA Format Processing Fragment size Typical recovery DNA quality Elution volume Preparation time Binding capacity NucleoSpin 96 DNA FFPE Silica-membrane technology 96-well plates Manual or automated, vacuum or centrifugation < 10 mg tissue, < 15 mg paraffin 50 bp approx. 5 kbp Depending on sample amount and quality Strongly depending on sample quality 100 μl 60 min/plate (excl. lysis) 20 μg Product Preps Specification REF NucleoSpin 96 DNA FFPE 1 x 96 4 x 96 NucleoSpin DNA FFPE Binding Plate, MN Wash Plate, MN Square-well Block, Round-well Block, Rack of Tube Strips, Self-adhering Foil, Paraffin Dissolver, buffers, Proteinase K Product accessories Pack of Specification REF NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator 1 for controlling of vacuum For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 /

11 Genomic DNA from FFPE samples NucleoMag DNA FFPE Isolation of genomic DNA from formalin-fixed, paraffin-embedded samples (fresh or archived) with magnetic-bead technology Blue colored Paraffin Dissolver (patent pending) for convenient paraffin removal no need for harmful organic solvents Removal of formalin crosslinking by efficient decrosslinking buffer high-quality DNA for improved performance in downstream applications (e.g., PCR) Magnetic-bead technology highest flexibility and easy adaption for automated use Format Processing Fragment size Typical recovery DNA quality Elution volume Preparation time Binding capacity NucleoMag DNA FFPE Magnetic-bead technology Highly reactive superparamagnetic beads Manual or automated < 5 mg tissue, < 15 mg paraffin 50 bp approx. 5 kbp Depending on amount and quality of sample Strongly depending on sample quality > 25 μl 120 min/96 preps 0.4 μg/μl beads Product Preps Specification REF NucleoMag DNA FFPE 1 x 96 4 x 96 NucleoMag B-Beads, buffers, Proteinase K Material to be supplied by the user Pack of Specification REF E.g., Rack of Tube Strips (lysis tubes) 4 sets 24 sets 1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 cap strips Square-well Block (separation plate) 4 24 E.g., Elution Plate U-bottom (elution plate) 96-well blocks with 2.1 ml square wells well microplates with 300 μl u-bottom wells Product accessories Pack of Specification REF NucleoMag SEP 1 magnetic separator, for use with 96-well plates NucleoMag SEP Mini 1 magnetic separator, for use with 1.5 ml or ml reaction tubes (12 positions) NucleoMag SEP Maxi 1 magnetic separator, for use with 50 ml tubes (4 positions) For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 / Genomic DNA 11

12 Genomic DNA from forensic samples NucleoSpin Forensic Filters Incubation and lysate separation of swabs, denim, cigarette butts, or other solid sample carriers Lysis and lysate separation in one tube no sample transfer, no extra pipetting steps Special filter design absolutely leak-proof and compatible with all typical lysis buffers Complete DNA recovery and maximum process reliability no DNA loss Collection Tube with lid no cross-contamination Maximum volume capacity Forensic quality NucleoSpin Forensic Filters Semi-permeable basket Swabs, denim, cigarette butts, others 800 μl Ethylene oxide treated Typical downstream applications DNA purification (e.g., with NucleoSpin Tissue, NucleoSpin Tissue XS, NucleoSpin 8 / 96 Trace, NucleoMag Forensic, NucleoMag 96 Trace) EO treated plastic materials Genomic DNA Procedure Sample preparation DNA purification Prepare Place sample into fi lter and add lysis buffer Incubate Nucleic acids are released without fi lter leakage Separate Spin fi lter to separate carrier and to collect lysate Follow up (e.g., NucleoSpin / NucleoMag DNA isolation kits) Product Preps Specification REF NucleoSpin Forensic Filters NucleoSpin Forensic Filters (Bulk) NucleoSpin Forensic Filters with Collection Tubes (2 ml), blistered, ethylene oxide treated NucleoSpin Forensic Filters, Collection Tubes (2 ml), bulk packed, ethylene oxide treated For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 / ** For more detailed information regarding the kits, please see the main Bioanalysis Catalog 2014 / 2015 or visit B B B 12

13 Viral RNA and DNA from serum and plasma NucleoSpin Virus One kit for viral RNA and DNA Purification of viral RNA and DNA from cell-free biological fluids (human or animal) Processing of up to 400 μl starting volume maximal recovery of viral nucleic acids Liquid Proteinase K included convenient and highly efficient sample lysis Format Fragment size Elution volume Preparation time Binding capacity Typical downstream applications NucleoSpin Virus Silica-membrane technology Mini spin columns < 200 μl serum, plasma, cell-free biological fluids (< 400 μl with two loading steps) 100 bp approx. 50 kbp 30 μl 50 min/6 preps 25 μg qrt-pcr, qpcr, enzymatic reactions Product Preps Specification REF NucleoSpin Virus NucleoSpin Virus Columns with Collection Tubes, Collection Tubes (2 ml and 1.5 ml), buffers, Carrier RNA, Liquid Proteinase K Product accessories Pack of REF Liquid Proteinase K 5 ml ready-to-use liquid Proteinase K, recombinant For separate kit components see Accessories page 149 of the main catalog Bioanalysis 2014 / Viral RNA and DNA 13

14 Accessories Equipment NucleoBond equipment Product Pack of Specification REF NucleoBond Smart Rack 1 for use with NucleoBond Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, AX 100, AX 500, AX 2000, and AX Columns NucleoSpin equipment Starter Set Midi 1 for processing NucleoSpin Midi / L Columns under vacuum on the NucleoVac 96 Vacuum Manifold or similar manifolds, contains 1 Column Holder Midi, 1 Wash Plate Midi, 1 Elution Tube Holder, 24 Dummy Columns Midi NucleoMag equipment NucleoMag SEP Mini 1 magnetic separator, for use with 1.5 ml or 2 ml reaction tubes (12 positions) NucleoMag SEP Maxi 1 magnetic separator, for use with 50 ml tubes (4 positions) Accessories Consumables Product Pack of Specification REF NucleoSpin consumables NucleoSpin Bead Tubes Type A 50 2 ml tubes with ceramic beads (as included in the NucleoSpin Soil kit) NucleoSpin Bead Tubes Type B 50 2 ml tubes with glass beads (as included in the NucleoSpin Microbial DNA kit) NucleoSpin Bead Tubes Type C 50 2 ml tubes with corundum (recommended for yeast disruption)

15 Product use restriction Trademarks used in this supplement MACHEREY-NAGEL trademarks NucleoBond kits and cartridges for nucleic acid purification (anion-exchange chromatography) NucleoMag kits for purification of DNA and RNA (magnetic-bead technology) NucleoSpin kits for purification of DNA and RNA (silica-membrane technology) Trademarks of other companies Registered trademarks Nalgene Thermo Fisher Scientific Inc. (USA) KingFisher Thermo Fisher Scientific Inc. (USA) Trademarks PCR is patented by Hoffmann La Roche Disclaimer All used names and denotations can be brands, trademarks, or registered labels of their respective owner also if they do not have a special denotation. To mention products and brands is only a kind of information, i.e., it does not offend against trademarks and brands and can not be seen as a kind of recommendation or assessment. Regarding these products or services, we can not grant any guarantees regarding selection, efficiency, or operation. Product use restriction / Warranty MACHEREY-NAGEL Bioanalysis products and their single components are intended, developed, designed, and sold FOR RESEARCH PURPOSES ONLY, except, however, any other function of the product being expressly described in original MACHEREY-NAGEL product leaflets. MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY! MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY! MACHEREY-NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING. For detailed information please refer to the respective Material Safety Data Sheet of the product! MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT. MACHEREY-NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application. Application on the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all damages resulting from such application. DNA / RNA / PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO-USES ONLY! ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable for IN-VITRO-diagnostic use. Please pay attention to the package of the product. IN-VITRO-DIAGNOSTIC products are expressly marked as IVD on the packaging. IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN-VITRO-DIAGNOSTIC USE! ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE (INCLUDING, BUT NOT LIMITED TO DIAGNOSTIC, THERAPEUTIC, AND / OR PROGNOSTIC USE). No claim or representations are intended for its use to identify any specific organism or for clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or blood banking). It is rather in the responsibility of the user or in any case of resale of the products in the responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification products of MACHEREY-NAGEL for a well-defined and specific application. MACHEREY-NAGEL shall only be responsible for the product specifications and the performance range of MN products according to the specifications of MN in-house quality control, product documentation, and marketing material. For detailed information on product use restriction and warranty, please refer to the respective user manuals. Product use restriction 15

16 DNA, RNA, and protein purification Catalog supplement 2016 Service Bioanalysis Technical Support and Customer Service phone Product Management phone Marketing Communication phone Sales phone OEM Sales phone Visit our Bioanalysis pages at +49 (0) (0) (0) (0) (0) (0) (0) (0) Your local distributor KATEN Catalog Supplement en3/8/0/ Printed in Germany MACHEREY-NAGEL EN ISO 9001: 2008 CERTIFIED MACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str Düren Germany Germany and international: Switzerland: MACHEREY-NAGEL AG France: MACHEREY-NAGEL EURL Tel.: Tel.: Tel.: Fax: Fax: Fax: info@mn-net.com sales-ch@mn-net.com sales-fr@mn-net.com USA: MACHEREY-NAGEL Inc. Tel.: Fax: sales-us@mn-net.com